ID   PUR5_ARATH              Reviewed;         389 AA.
AC   Q05728; Q9M2W5; Q9SV49;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   27-MAY-2002, sequence version 2.
DT   16-JUN-2009, entry version 79.
DE   RecName: Full=Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic;
DE            EC=6.3.3.1;
DE   AltName: Full=Phosphoribosyl-aminoimidazole synthetase;
DE   AltName: Full=AIR synthase;
DE            Short=AIRS;
DE   Flags: Precursor;
GN   Name=PUR5; OrderedLocusNames=At3g55010; ORFNames=T15C9.10, F28P10.10;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Columbia; TISSUE=Leaf, and Stem;
RX   MEDLINE=94151435; PubMed=8108507; DOI=10.1104/pp.102.2.387;
RA   Senecoff J.F., Meagher R.B.;
RT   "Isolating the Arabidopsis thaliana genes for de novo purine synthesis
RT   by suppression of Escherichia coli mutants. I. 5'-Phosphoribosyl-5-
RT   aminoimidazole synthetase.";
RL   Plant Physiol. 102:387-399(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016720; PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M.,
RA   Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B.,
RA   Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P.,
RA   De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P.,
RA   Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V.,
RA   Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S.,
RA   Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G.,
RA   Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B.,
RA   Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G.,
RA   Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J.,
RA   Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D.,
RA   Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D.,
RA   Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W.,
RA   Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J.,
RA   Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P.,
RA   Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S.,
RA   Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V.,
RA   Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C.,
RA   Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E.,
RA   Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y.,
RA   Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
RA   Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- CATALYTIC ACTIVITY: ATP + 2-(formamido)-N(1)-(5-phospho-D-
CC       ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-
CC       ribosyl)imidazole.
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-
CC       formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/5.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the AIR synthase family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC37341.1; Type=Frameshift; Positions=344;
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DR   EMBL; L12457; AAC37341.1; ALT_FRAME; Unassigned_DNA.
DR   EMBL; AL132970; CAB82696.1; -; Genomic_DNA.
DR   EMBL; AL049655; CAB41083.1; -; Genomic_DNA.
DR   EMBL; AY060585; AAL31210.1; -; mRNA.
DR   EMBL; AY142054; AAM98318.1; -; mRNA.
DR   IPI; IPI00523330; -.
DR   PIR; JQ2256; JQ2256.
DR   PIR; T47640; T47640.
DR   RefSeq; NP_191061.1; -.
DR   RefSeq; NP_974437.1; -.
DR   UniGene; At.1281; -.
DR   HSSP; P08178; 1CLI.
DR   PRIDE; Q05728; -.
DR   GeneID; 824667; -.
DR   GenomeReviews; BA000014_GR; AT3G55010.
DR   KEGG; ath:AT3G55010; -.
DR   NMPDR; fig|3702.1.peg.16813; -.
DR   TAIR; At3g55010; -.
DR   OMA; Q05728; VHGLAHI.
DR   BRENDA; 6.3.3.1; 302.
DR   ArrayExpress; Q05728; -.
DR   GermOnline; AT3G55010; Arabidopsis thaliana.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR   GO; GO:0005524; F:ATP binding; IDA:TAIR.
DR   GO; GO:0004641; F:phosphoribosylformylglycinamidine cyclo-lig...; IGI:TAIR.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:InterPro.
DR   InterPro; IPR000728; AIR_synth.
DR   InterPro; IPR010918; AIR_synth_C.
DR   InterPro; IPR004733; PurM_cligase.
DR   Pfam; PF00586; AIRS; 1.
DR   Pfam; PF02769; AIRS_C; 1.
DR   TIGRFAMs; TIGR00878; purM; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chloroplast; Complete proteome; Ligase;
KW   Nucleotide-binding; Plastid; Purine biosynthesis; Transit peptide.
FT   TRANSIT       1     58       Chloroplast (Potential).
FT   CHAIN        59    389       Phosphoribosylformylglycinamidine cyclo-
FT                                ligase, chloroplastic.
FT                                /FTId=PRO_0000029881.
SQ   SEQUENCE   389 AA;  41504 MW;  F341E9D21E9286F0 CRC64;
     MEARILQSSS SCYSSLYAVN RSRFSSVSSP KPFSVSFAQT TRTRTRVLSM SKKDGRTDKD
     DDTDSLNYKD SGVDIDAGAE LVKRIAKMAP GIGGFGGLFP LGDSYLVAGT DGVGTKLKLA
     FETGIHDTIG IDLVAMSVND IITSGAKPLF FLDYFATSRL DVDLAEKVIK GIVEGCRQSE
     CALLGGETAE MPDFYAEGEY DLSGFAVGIV KKTSVINGKN IVAGDVLIGL PSSGVHSNGF
     SLVRRVLARS NLSLKDALPG GSSTLGDALM APTVIYVKQV LDMIEKGGVK GLAHITGGGF
     TDNIPRVFPD GLGAVIHTDA WELPPLFKWI QQTGRIEDSE MRRTFNLGIG MVMVVSPEAA
     SRILEEVKNG DYVAYRVGEV VNGEGVSYQ
//