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Protein

Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic

Gene

PUR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrial (At1g74260)
  2. Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic (PUR5)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

  • ATP binding Source: TAIR
  • copper ion binding Source: TAIR
  • phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G55010-MONOMER
UniPathwayiUPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:PUR5
Ordered Locus Names:At3g55010
ORF Names:F28P10.10, T15C9.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G55010
TAIRilocus:2097203 AT3G55010

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 58ChloroplastSequence analysisAdd BLAST58
ChainiPRO_000002988159 – 389Phosphoribosylformylglycinamidine cyclo-ligase, chloroplasticAdd BLAST331

Proteomic databases

PaxDbiQ05728
PRIDEiQ05728

Expressioni

Gene expression databases

ExpressionAtlasiQ05728 baseline and differential
GenevisibleiQ05728 AT

Interactioni

Protein-protein interaction databases

BioGridi9983, 2 interactors
IntActiQ05728, 1 interactor
STRINGi3702.AT3G55010.1

Structurei

3D structure databases

ProteinModelPortaliQ05728
SMRiQ05728
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410INX0 Eukaryota
COG0150 LUCA
HOGENOMiHOG000229091
InParanoidiQ05728
KOiK01933
OMAiNHCVNDI
OrthoDBiEOG09360C2K
PhylomeDBiQ05728

Family and domain databases

CDDicd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
InterProiView protein in InterPro
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
PANTHERiPTHR10520 PTHR10520, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
SUPFAMiSSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00878 purM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEARILQSSS SCYSSLYAVN RSRFSSVSSP KPFSVSFAQT TRTRTRVLSM
60 70 80 90 100
SKKDGRTDKD DDTDSLNYKD SGVDIDAGAE LVKRIAKMAP GIGGFGGLFP
110 120 130 140 150
LGDSYLVAGT DGVGTKLKLA FETGIHDTIG IDLVAMSVND IITSGAKPLF
160 170 180 190 200
FLDYFATSRL DVDLAEKVIK GIVEGCRQSE CALLGGETAE MPDFYAEGEY
210 220 230 240 250
DLSGFAVGIV KKTSVINGKN IVAGDVLIGL PSSGVHSNGF SLVRRVLARS
260 270 280 290 300
NLSLKDALPG GSSTLGDALM APTVIYVKQV LDMIEKGGVK GLAHITGGGF
310 320 330 340 350
TDNIPRVFPD GLGAVIHTDA WELPPLFKWI QQTGRIEDSE MRRTFNLGIG
360 370 380
MVMVVSPEAA SRILEEVKNG DYVAYRVGEV VNGEGVSYQ
Length:389
Mass (Da):41,504
Last modified:May 27, 2002 - v2
Checksum:iF341E9D21E9286F0
GO

Sequence cautioni

The sequence AAC37341 differs from that shown. Reason: Frameshift at position 344.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12457 Unassigned DNA Translation: AAC37341.1 Frameshift.
AL049655 Genomic DNA Translation: CAB41083.1
AL132970 Genomic DNA Translation: CAB82696.1
CP002686 Genomic DNA Translation: AEE79327.1
CP002686 Genomic DNA Translation: AEE79328.1
AY060585 mRNA Translation: AAL31210.1
AY142054 mRNA Translation: AAM98318.1
PIRiJQ2256
T47640
RefSeqiNP_191061.1, NM_115359.3
NP_974437.1, NM_202708.2
UniGeneiAt.1281

Genome annotation databases

EnsemblPlantsiAT3G55010.1; AT3G55010.1; AT3G55010
AT3G55010.2; AT3G55010.2; AT3G55010
GeneIDi824667
GrameneiAT3G55010.1; AT3G55010.1; AT3G55010
AT3G55010.2; AT3G55010.2; AT3G55010
KEGGiath:AT3G55010

Similar proteinsi

Entry informationi

Entry nameiPUR5_ARATH
AccessioniPrimary (citable) accession number: Q05728
Secondary accession number(s): Q9M2W5, Q9SV49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 27, 2002
Last modified: April 25, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health