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Q05728

- PUR5_ARATH

UniProt

Q05728 - PUR5_ARATH

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Protein
Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic
Gene
PUR5, At3g55010, F28P10.10, T15C9.10
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: TAIR
  2. copper ion binding Source: TAIR
  3. phosphoribosylformylglycinamidine cyclo-ligase activity Source: TAIR

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleotide biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G55010-MONOMER.
ARA:GQT-2720-MONOMER.
UniPathwayiUPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:PUR5
Ordered Locus Names:At3g55010
ORF Names:F28P10.10, T15C9.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G55010.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5858Chloroplast Reviewed prediction
Add
BLAST
Chaini59 – 389331Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic
PRO_0000029881Add
BLAST

Proteomic databases

PaxDbiQ05728.
PRIDEiQ05728.

Expressioni

Gene expression databases

GenevestigatoriQ05728.

Interactioni

Protein-protein interaction databases

BioGridi9983. 1 interaction.
IntActiQ05728. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ05728.
SMRiQ05728. Positions 65-378.

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0150.
HOGENOMiHOG000229091.
InParanoidiQ05728.
KOiK01933.
OMAiVIGKIEH.
PhylomeDBiQ05728.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05728-1 [UniParc]FASTAAdd to Basket

« Hide

MEARILQSSS SCYSSLYAVN RSRFSSVSSP KPFSVSFAQT TRTRTRVLSM    50
SKKDGRTDKD DDTDSLNYKD SGVDIDAGAE LVKRIAKMAP GIGGFGGLFP 100
LGDSYLVAGT DGVGTKLKLA FETGIHDTIG IDLVAMSVND IITSGAKPLF 150
FLDYFATSRL DVDLAEKVIK GIVEGCRQSE CALLGGETAE MPDFYAEGEY 200
DLSGFAVGIV KKTSVINGKN IVAGDVLIGL PSSGVHSNGF SLVRRVLARS 250
NLSLKDALPG GSSTLGDALM APTVIYVKQV LDMIEKGGVK GLAHITGGGF 300
TDNIPRVFPD GLGAVIHTDA WELPPLFKWI QQTGRIEDSE MRRTFNLGIG 350
MVMVVSPEAA SRILEEVKNG DYVAYRVGEV VNGEGVSYQ 389
Length:389
Mass (Da):41,504
Last modified:May 27, 2002 - v2
Checksum:iF341E9D21E9286F0
GO

Sequence cautioni

The sequence AAC37341.1 differs from that shown. Reason: Frameshift at position 344.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12457 Unassigned DNA. Translation: AAC37341.1. Frameshift.
AL049655 Genomic DNA. Translation: CAB41083.1.
AL132970 Genomic DNA. Translation: CAB82696.1.
CP002686 Genomic DNA. Translation: AEE79327.1.
CP002686 Genomic DNA. Translation: AEE79328.1.
AY060585 mRNA. Translation: AAL31210.1.
AY142054 mRNA. Translation: AAM98318.1.
PIRiJQ2256.
T47640.
RefSeqiNP_191061.1. NM_115359.2.
NP_974437.1. NM_202708.1.
UniGeneiAt.1281.

Genome annotation databases

EnsemblPlantsiAT3G55010.1; AT3G55010.1; AT3G55010.
AT3G55010.2; AT3G55010.2; AT3G55010.
GeneIDi824667.
KEGGiath:AT3G55010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12457 Unassigned DNA. Translation: AAC37341.1 . Frameshift.
AL049655 Genomic DNA. Translation: CAB41083.1 .
AL132970 Genomic DNA. Translation: CAB82696.1 .
CP002686 Genomic DNA. Translation: AEE79327.1 .
CP002686 Genomic DNA. Translation: AEE79328.1 .
AY060585 mRNA. Translation: AAL31210.1 .
AY142054 mRNA. Translation: AAM98318.1 .
PIRi JQ2256.
T47640.
RefSeqi NP_191061.1. NM_115359.2.
NP_974437.1. NM_202708.1.
UniGenei At.1281.

3D structure databases

ProteinModelPortali Q05728.
SMRi Q05728. Positions 65-378.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 9983. 1 interaction.
IntActi Q05728. 1 interaction.

Proteomic databases

PaxDbi Q05728.
PRIDEi Q05728.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G55010.1 ; AT3G55010.1 ; AT3G55010 .
AT3G55010.2 ; AT3G55010.2 ; AT3G55010 .
GeneIDi 824667.
KEGGi ath:AT3G55010.

Organism-specific databases

TAIRi AT3G55010.

Phylogenomic databases

eggNOGi COG0150.
HOGENOMi HOG000229091.
InParanoidi Q05728.
KOi K01933.
OMAi VIGKIEH.
PhylomeDBi Q05728.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00129 .
BioCyci ARA:AT3G55010-MONOMER.
ARA:GQT-2720-MONOMER.

Miscellaneous databases

PROi Q05728.

Gene expression databases

Genevestigatori Q05728.

Family and domain databases

Gene3Di 3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsi TIGR00878. purM. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolating the Arabidopsis thaliana genes for de novo purine synthesis by suppression of Escherichia coli mutants. I. 5'-Phosphoribosyl-5-aminoimidazole synthetase."
    Senecoff J.F., Meagher R.B.
    Plant Physiol. 102:387-399(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Columbia.
    Tissue: Leaf and Stem.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPUR5_ARATH
AccessioniPrimary (citable) accession number: Q05728
Secondary accession number(s): Q9M2W5, Q9SV49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 27, 2002
Last modified: May 14, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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