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Protein

Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic

Gene

PUR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi

GO - Molecular functioni

  • ATP binding Source: TAIR
  • copper ion binding Source: TAIR
  • phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G55010-MONOMER.
ARA:GQT-2720-MONOMER.
UniPathwayiUPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:PUR5
Ordered Locus Names:At3g55010
ORF Names:F28P10.10, T15C9.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G55010.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5858ChloroplastSequence AnalysisAdd
BLAST
Chaini59 – 389331Phosphoribosylformylglycinamidine cyclo-ligase, chloroplasticPRO_0000029881Add
BLAST

Proteomic databases

PaxDbiQ05728.
PRIDEiQ05728.

Expressioni

Gene expression databases

GenevestigatoriQ05728.

Interactioni

Protein-protein interaction databases

BioGridi9983. 1 interaction.
IntActiQ05728. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ05728.
SMRiQ05728. Positions 65-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0150.
HOGENOMiHOG000229091.
InParanoidiQ05728.
KOiK01933.
OMAiNHCVNDI.
PhylomeDBiQ05728.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEARILQSSS SCYSSLYAVN RSRFSSVSSP KPFSVSFAQT TRTRTRVLSM
60 70 80 90 100
SKKDGRTDKD DDTDSLNYKD SGVDIDAGAE LVKRIAKMAP GIGGFGGLFP
110 120 130 140 150
LGDSYLVAGT DGVGTKLKLA FETGIHDTIG IDLVAMSVND IITSGAKPLF
160 170 180 190 200
FLDYFATSRL DVDLAEKVIK GIVEGCRQSE CALLGGETAE MPDFYAEGEY
210 220 230 240 250
DLSGFAVGIV KKTSVINGKN IVAGDVLIGL PSSGVHSNGF SLVRRVLARS
260 270 280 290 300
NLSLKDALPG GSSTLGDALM APTVIYVKQV LDMIEKGGVK GLAHITGGGF
310 320 330 340 350
TDNIPRVFPD GLGAVIHTDA WELPPLFKWI QQTGRIEDSE MRRTFNLGIG
360 370 380
MVMVVSPEAA SRILEEVKNG DYVAYRVGEV VNGEGVSYQ
Length:389
Mass (Da):41,504
Last modified:May 27, 2002 - v2
Checksum:iF341E9D21E9286F0
GO

Sequence cautioni

The sequence AAC37341.1 differs from that shown. Reason: Frameshift at position 344. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12457 Unassigned DNA. Translation: AAC37341.1. Frameshift.
AL049655 Genomic DNA. Translation: CAB41083.1.
AL132970 Genomic DNA. Translation: CAB82696.1.
CP002686 Genomic DNA. Translation: AEE79327.1.
CP002686 Genomic DNA. Translation: AEE79328.1.
AY060585 mRNA. Translation: AAL31210.1.
AY142054 mRNA. Translation: AAM98318.1.
PIRiJQ2256.
T47640.
RefSeqiNP_191061.1. NM_115359.2.
NP_974437.1. NM_202708.1.
UniGeneiAt.1281.

Genome annotation databases

EnsemblPlantsiAT3G55010.1; AT3G55010.1; AT3G55010.
AT3G55010.2; AT3G55010.2; AT3G55010.
GeneIDi824667.
KEGGiath:AT3G55010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12457 Unassigned DNA. Translation: AAC37341.1. Frameshift.
AL049655 Genomic DNA. Translation: CAB41083.1.
AL132970 Genomic DNA. Translation: CAB82696.1.
CP002686 Genomic DNA. Translation: AEE79327.1.
CP002686 Genomic DNA. Translation: AEE79328.1.
AY060585 mRNA. Translation: AAL31210.1.
AY142054 mRNA. Translation: AAM98318.1.
PIRiJQ2256.
T47640.
RefSeqiNP_191061.1. NM_115359.2.
NP_974437.1. NM_202708.1.
UniGeneiAt.1281.

3D structure databases

ProteinModelPortaliQ05728.
SMRiQ05728. Positions 65-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9983. 1 interaction.
IntActiQ05728. 1 interaction.

Proteomic databases

PaxDbiQ05728.
PRIDEiQ05728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G55010.1; AT3G55010.1; AT3G55010.
AT3G55010.2; AT3G55010.2; AT3G55010.
GeneIDi824667.
KEGGiath:AT3G55010.

Organism-specific databases

TAIRiAT3G55010.

Phylogenomic databases

eggNOGiCOG0150.
HOGENOMiHOG000229091.
InParanoidiQ05728.
KOiK01933.
OMAiNHCVNDI.
PhylomeDBiQ05728.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00129.
BioCyciARA:AT3G55010-MONOMER.
ARA:GQT-2720-MONOMER.

Miscellaneous databases

PROiQ05728.

Gene expression databases

GenevestigatoriQ05728.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolating the Arabidopsis thaliana genes for de novo purine synthesis by suppression of Escherichia coli mutants. I. 5'-Phosphoribosyl-5-aminoimidazole synthetase."
    Senecoff J.F., Meagher R.B.
    Plant Physiol. 102:387-399(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Columbia.
    Tissue: Leaf and Stem.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPUR5_ARATH
AccessioniPrimary (citable) accession number: Q05728
Secondary accession number(s): Q9M2W5, Q9SV49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 27, 2002
Last modified: April 1, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.