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Q05728 (PUR5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic

EC=6.3.3.1
Alternative name(s):
AIR synthase
Short name=AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene names
Name:PUR5
Ordered Locus Names:At3g55010
ORF Names:F28P10.10, T15C9.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the AIR synthase family.

Sequence caution

The sequence AAC37341.1 differs from that shown. Reason: Frameshift at position 344.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5858Chloroplast Potential
Chain59 – 389331Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic
PRO_0000029881

Sequences

Sequence LengthMass (Da)Tools
Q05728 [UniParc].

Last modified May 27, 2002. Version 2.
Checksum: F341E9D21E9286F0

FASTA38941,504
        10         20         30         40         50         60 
MEARILQSSS SCYSSLYAVN RSRFSSVSSP KPFSVSFAQT TRTRTRVLSM SKKDGRTDKD 

        70         80         90        100        110        120 
DDTDSLNYKD SGVDIDAGAE LVKRIAKMAP GIGGFGGLFP LGDSYLVAGT DGVGTKLKLA 

       130        140        150        160        170        180 
FETGIHDTIG IDLVAMSVND IITSGAKPLF FLDYFATSRL DVDLAEKVIK GIVEGCRQSE 

       190        200        210        220        230        240 
CALLGGETAE MPDFYAEGEY DLSGFAVGIV KKTSVINGKN IVAGDVLIGL PSSGVHSNGF 

       250        260        270        280        290        300 
SLVRRVLARS NLSLKDALPG GSSTLGDALM APTVIYVKQV LDMIEKGGVK GLAHITGGGF 

       310        320        330        340        350        360 
TDNIPRVFPD GLGAVIHTDA WELPPLFKWI QQTGRIEDSE MRRTFNLGIG MVMVVSPEAA 

       370        380 
SRILEEVKNG DYVAYRVGEV VNGEGVSYQ 

« Hide

References

« Hide 'large scale' references
[1]"Isolating the Arabidopsis thaliana genes for de novo purine synthesis by suppression of Escherichia coli mutants. I. 5'-Phosphoribosyl-5-aminoimidazole synthetase."
Senecoff J.F., Meagher R.B.
Plant Physiol. 102:387-399(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Columbia.
Tissue: Leaf and Stem.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12457 Unassigned DNA. Translation: AAC37341.1. Frameshift.
AL049655 Genomic DNA. Translation: CAB41083.1.
AL132970 Genomic DNA. Translation: CAB82696.1.
CP002686 Genomic DNA. Translation: AEE79327.1.
CP002686 Genomic DNA. Translation: AEE79328.1.
AY060585 mRNA. Translation: AAL31210.1.
AY142054 mRNA. Translation: AAM98318.1.
PIRJQ2256.
T47640.
RefSeqNP_191061.1. NM_115359.2.
NP_974437.1. NM_202708.1.
UniGeneAt.1281.

3D structure databases

ProteinModelPortalQ05728.
SMRQ05728. Positions 65-378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid9983. 1 interaction.
IntActQ05728. 1 interaction.

Proteomic databases

PaxDbQ05728.
PRIDEQ05728.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G55010.1; AT3G55010.1; AT3G55010.
AT3G55010.2; AT3G55010.2; AT3G55010.
GeneID824667.
KEGGath:AT3G55010.

Organism-specific databases

TAIRAT3G55010.

Phylogenomic databases

eggNOGCOG0150.
HOGENOMHOG000229091.
InParanoidQ05728.
KOK01933.
OMAVIGKIEH.
PhylomeDBQ05728.

Enzyme and pathway databases

BioCycARA:AT3G55010-MONOMER.
ARA:GQT-2720-MONOMER.
UniPathwayUPA00074; UER00129.

Gene expression databases

GenevestigatorQ05728.

Family and domain databases

Gene3D3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsTIGR00878. purM. 1 hit.
ProtoNetSearch...

Other

PROQ05728.

Entry information

Entry namePUR5_ARATH
AccessionPrimary (citable) accession number: Q05728
Secondary accession number(s): Q9M2W5, Q9SV49
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 27, 2002
Last modified: May 14, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names