Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q05728

- PUR5_ARATH

UniProt

Q05728 - PUR5_ARATH

Protein

Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic

Gene

PUR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: TAIR
    2. copper ion binding Source: TAIR
    3. phosphoribosylformylglycinamidine cyclo-ligase activity Source: TAIR

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleotide biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G55010-MONOMER.
    ARA:GQT-2720-MONOMER.
    UniPathwayiUPA00074; UER00129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic (EC:6.3.3.1)
    Alternative name(s):
    AIR synthase
    Short name:
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    Gene namesi
    Name:PUR5
    Ordered Locus Names:At3g55010
    ORF Names:F28P10.10, T15C9.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G55010.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5858ChloroplastSequence AnalysisAdd
    BLAST
    Chaini59 – 389331Phosphoribosylformylglycinamidine cyclo-ligase, chloroplasticPRO_0000029881Add
    BLAST

    Proteomic databases

    PaxDbiQ05728.
    PRIDEiQ05728.

    Expressioni

    Gene expression databases

    GenevestigatoriQ05728.

    Interactioni

    Protein-protein interaction databases

    BioGridi9983. 1 interaction.
    IntActiQ05728. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05728.
    SMRiQ05728. Positions 65-378.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AIR synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0150.
    HOGENOMiHOG000229091.
    InParanoidiQ05728.
    KOiK01933.
    OMAiVIGKIEH.
    PhylomeDBiQ05728.

    Family and domain databases

    Gene3Di3.30.1330.10. 1 hit.
    3.90.650.10. 1 hit.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsiTIGR00878. purM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05728-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEARILQSSS SCYSSLYAVN RSRFSSVSSP KPFSVSFAQT TRTRTRVLSM    50
    SKKDGRTDKD DDTDSLNYKD SGVDIDAGAE LVKRIAKMAP GIGGFGGLFP 100
    LGDSYLVAGT DGVGTKLKLA FETGIHDTIG IDLVAMSVND IITSGAKPLF 150
    FLDYFATSRL DVDLAEKVIK GIVEGCRQSE CALLGGETAE MPDFYAEGEY 200
    DLSGFAVGIV KKTSVINGKN IVAGDVLIGL PSSGVHSNGF SLVRRVLARS 250
    NLSLKDALPG GSSTLGDALM APTVIYVKQV LDMIEKGGVK GLAHITGGGF 300
    TDNIPRVFPD GLGAVIHTDA WELPPLFKWI QQTGRIEDSE MRRTFNLGIG 350
    MVMVVSPEAA SRILEEVKNG DYVAYRVGEV VNGEGVSYQ 389
    Length:389
    Mass (Da):41,504
    Last modified:May 27, 2002 - v2
    Checksum:iF341E9D21E9286F0
    GO

    Sequence cautioni

    The sequence AAC37341.1 differs from that shown. Reason: Frameshift at position 344.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12457 Unassigned DNA. Translation: AAC37341.1. Frameshift.
    AL049655 Genomic DNA. Translation: CAB41083.1.
    AL132970 Genomic DNA. Translation: CAB82696.1.
    CP002686 Genomic DNA. Translation: AEE79327.1.
    CP002686 Genomic DNA. Translation: AEE79328.1.
    AY060585 mRNA. Translation: AAL31210.1.
    AY142054 mRNA. Translation: AAM98318.1.
    PIRiJQ2256.
    T47640.
    RefSeqiNP_191061.1. NM_115359.2.
    NP_974437.1. NM_202708.1.
    UniGeneiAt.1281.

    Genome annotation databases

    EnsemblPlantsiAT3G55010.1; AT3G55010.1; AT3G55010.
    AT3G55010.2; AT3G55010.2; AT3G55010.
    GeneIDi824667.
    KEGGiath:AT3G55010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12457 Unassigned DNA. Translation: AAC37341.1 . Frameshift.
    AL049655 Genomic DNA. Translation: CAB41083.1 .
    AL132970 Genomic DNA. Translation: CAB82696.1 .
    CP002686 Genomic DNA. Translation: AEE79327.1 .
    CP002686 Genomic DNA. Translation: AEE79328.1 .
    AY060585 mRNA. Translation: AAL31210.1 .
    AY142054 mRNA. Translation: AAM98318.1 .
    PIRi JQ2256.
    T47640.
    RefSeqi NP_191061.1. NM_115359.2.
    NP_974437.1. NM_202708.1.
    UniGenei At.1281.

    3D structure databases

    ProteinModelPortali Q05728.
    SMRi Q05728. Positions 65-378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 9983. 1 interaction.
    IntActi Q05728. 1 interaction.

    Proteomic databases

    PaxDbi Q05728.
    PRIDEi Q05728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G55010.1 ; AT3G55010.1 ; AT3G55010 .
    AT3G55010.2 ; AT3G55010.2 ; AT3G55010 .
    GeneIDi 824667.
    KEGGi ath:AT3G55010.

    Organism-specific databases

    TAIRi AT3G55010.

    Phylogenomic databases

    eggNOGi COG0150.
    HOGENOMi HOG000229091.
    InParanoidi Q05728.
    KOi K01933.
    OMAi VIGKIEH.
    PhylomeDBi Q05728.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00129 .
    BioCyci ARA:AT3G55010-MONOMER.
    ARA:GQT-2720-MONOMER.

    Miscellaneous databases

    PROi Q05728.

    Gene expression databases

    Genevestigatori Q05728.

    Family and domain databases

    Gene3Di 3.30.1330.10. 1 hit.
    3.90.650.10. 1 hit.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsi TIGR00878. purM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolating the Arabidopsis thaliana genes for de novo purine synthesis by suppression of Escherichia coli mutants. I. 5'-Phosphoribosyl-5-aminoimidazole synthetase."
      Senecoff J.F., Meagher R.B.
      Plant Physiol. 102:387-399(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Columbia.
      Tissue: Leaf and Stem.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPUR5_ARATH
    AccessioniPrimary (citable) accession number: Q05728
    Secondary accession number(s): Q9M2W5, Q9SV49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3