Reviewed, UniProtKB/Swiss-Prot Q05728 (PUR5_ARATH)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic
    EC=6.3.3.1
Alternative name(s):
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase
      Short name=AIRS

Gene names

Name:	PUR5
Ordered Locus Names:	At3g55010
ORF Names:	T15C9.10, F28P10.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/5.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the AIR synthase family.
Sequence caution	The sequence AAC37341.1 differs from that shown. Reason: Frameshift at position 344.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from direct assay. Source: TAIR mitochondrion Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from direct assay. Source: TAIR phosphoribosylformylglycinamidine cyclo-ligase activity Ref.1 Inferred from genetic interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 58	58	Chloroplast Potential
	Chain	59 – 389	331	Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic		PRO_0000029881

Sequences

	Sequence		Length	Mass (Da)
	Q05728-1 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: F341E9D21E9286F0	FASTA	389	41,504
	10 20 30 40 50 60 MEARILQSSS SCYSSLYAVN RSRFSSVSSP KPFSVSFAQT TRTRTRVLSM SKKDGRTDKD 70 80 90 100 110 120 DDTDSLNYKD SGVDIDAGAE LVKRIAKMAP GIGGFGGLFP LGDSYLVAGT DGVGTKLKLA 130 140 150 160 170 180 FETGIHDTIG IDLVAMSVND IITSGAKPLF FLDYFATSRL DVDLAEKVIK GIVEGCRQSE 190 200 210 220 230 240 CALLGGETAE MPDFYAEGEY DLSGFAVGIV KKTSVINGKN IVAGDVLIGL PSSGVHSNGF 250 260 270 280 290 300 SLVRRVLARS NLSLKDALPG GSSTLGDALM APTVIYVKQV LDMIEKGGVK GLAHITGGGF 310 320 330 340 350 360 TDNIPRVFPD GLGAVIHTDA WELPPLFKWI QQTGRIEDSE MRRTFNLGIG MVMVVSPEAA 370 380 SRILEEVKNG DYVAYRVGEV VNGEGVSYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolating the Arabidopsis thaliana genes for de novo purine synthesis by suppression of Escherichia coli mutants. I. 5'-Phosphoribosyl-5-aminoimidazole synthetase." Senecoff J.F., Meagher R.B. Plant Physiol. 102:387-399(1993) [PubMed: 8108507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia. Tissue: Leaf and Stem.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12457 Unassigned DNA. Translation: AAC37341.1. Frameshift. AL132970 Genomic DNA. Translation: CAB82696.1. AL049655 Genomic DNA. Translation: CAB41083.1. AY060585 mRNA. Translation: AAL31210.1. AY142054 mRNA. Translation: AAM98318.1.
IPI	IPI00523330.
PIR	JQ2256. T47640.
RefSeq	NP_191061.1. NP_974437.1.
UniGene	At.1281
3D structure databases
HSSP	HSSP built from PDB template 1CLI based on UniProtKB P08178.
ModBase	Search...
Proteomic databases
PRIDE	Q05728.
Genome annotation databases
GeneID	824667.
GenomeReviews	Gene locus AT3G55010 in contig BA000014_GR.
KEGG	ath:AT3G55010.
NMPDR	fig|3702.1.peg.16813.
Organism-specific databases
TAIR	At3g55010.
Phylogenomic databases
OMA	Q05728. VHGLAHI.
Enzyme and pathway databases
BRENDA	6.3.3.1. 302.
Gene expression databases
ArrayExpress	Q05728.
GermOnline	AT3G55010. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_ARATH

Accession

Primary (citable) accession number: Q05728
Secondary accession number(s): Q9M2W5, Q9SV49

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	May 27, 2002
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)