Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q05722

- CO9A1_MOUSE

UniProt

Q05722 - CO9A1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Collagen alpha-1(IX) chain

Gene

Col9a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Structural component of hyaline cartilage and vitreous of the eye.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi213 – 2131ZincBy similarity
Metal bindingi215 – 2151ZincBy similarity
Metal bindingi253 – 2531ZincBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cartilage development Source: MGI
  2. growth plate cartilage development Source: MGI
  3. tissue homeostasis Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen alpha-1(IX) chain
Gene namesi
Name:Col9a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:88465. Col9a1.

Subcellular locationi

GO - Cellular componenti

  1. collagen trimer Source: UniProtKB-KW
  2. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 921898Collagen alpha-1(IX) chainPRO_0000005766Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 242By similarity
Disulfide bondi198 ↔ 252By similarity
Disulfide bondi411 – 411InterchainBy similarity
Disulfide bondi415 – 415InterchainBy similarity

Post-translational modificationi

Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Keywords - PTMi

Disulfide bond, Hydroxylation

Proteomic databases

PaxDbiQ05722.
PRIDEiQ05722.

PTM databases

PhosphoSiteiQ05722.

Expressioni

Gene expression databases

CleanExiMM_COL9A1.
GenevestigatoriQ05722.

Interactioni

Subunit structurei

Heterotrimer of an alpha 1(IX), an alpha 2(IX) and an alpha 3(IX) chain.

Structurei

3D structure databases

ProteinModelPortaliQ05722.
SMRiQ05722. Positions 42-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 244195Laminin G-likeAdd
BLAST
Domaini269 – 32557Collagen-like 1Add
BLAST
Domaini326 – 35631Collagen-like 2Add
BLAST
Domaini358 – 40346Collagen-like 3Add
BLAST
Domaini416 – 47257Collagen-like 4Add
BLAST
Domaini473 – 51240Collagen-like 5Add
BLAST
Domaini604 – 65653Collagen-like 6Add
BLAST
Domaini657 – 71155Collagen-like 7Add
BLAST
Domaini712 – 75544Collagen-like 8Add
BLAST
Domaini790 – 84758Collagen-like 9Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 268245Nonhelical region (NC4)Add
BLAST
Regioni269 – 405137Triple-helical region (COL3)Add
BLAST
Regioni406 – 41712Nonhelical region (NC3)Add
BLAST
Regioni418 – 756339Triple-helical region (COL2)Add
BLAST
Regioni757 – 78630Nonhelical region (NC2)Add
BLAST
Regioni787 – 901115Triple-helical region (COL1)Add
BLAST
Regioni902 – 92120Nonhelical region (NC1)Add
BLAST

Domaini

Each subunit is composed of three triple-helical domains interspersed with non-collagenous domains. The globular domain at the N-terminus of type IX collagen molecules represents the NC4 domain which may participate in electrostatic interactions with polyanionic glycosaminoglycans in cartilage.

Sequence similaritiesi

Contains 9 collagen-like domains.Curated
Contains 1 laminin G-like domain.Curated

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000085653.
HOVERGENiHBG004933.
InParanoidiQ05722.
KOiK08131.
PhylomeDBiQ05722.

Family and domain databases

InterProiIPR008160. Collagen.
IPR013320. ConA-like_dom.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF01391. Collagen. 9 hits.
[Graphical view]
SMARTiSM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform Long (identifier: Q05722-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKNFWKISVF FCVCSCLGPW VSATLKRRAR FPANSISNGG SELCPKIRIG
60 70 80 90 100
QDDLPGFDLI SQFQIEKAAS RRTIQRVVGS TALQVAYKLG SNVDFRIPTR
110 120 130 140 150
HLYPSGLPEE YSFLTTFRMT GSTLEKHWNI WQIQDSAGRE QVGVKINGQT
160 170 180 190 200
KSVAFSYKGL DGSLQTAAFL NLPSLFDSRW HKLMIGVERT SATLFIDCIR
210 220 230 240 250
IESLPIKPRG QIDADGFAVL GKLVDNPQVS VPFELQWMLI HCDPLRPRRE
260 270 280 290 300
TCHELPIRIT TSQTTDERGP PGEQGPPGPP GPPGVPGIDG IDGDRGPKGP
310 320 330 340 350
PGPPGPPGDP GKPGAPGKPG TPGADGLTGP DGSPGSVGPR GQKGEPGVPG
360 370 380 390 400
SRGFPGRGIP GPPGPPGTTG LPGELGRVGP IGDPGKRGPP GPPGPPGPSG
410 420 430 440 450
TIGFHDGDPL CPNSCPPGRS GYPGLPGMRG HKGAKGEIGE PGRQGHKGEE
460 470 480 490 500
GDQGELGEVG AQGPPGPQGL RGITGIVGDK GEKGARGFDG EPGPQGIPGA
510 520 530 540 550
AGDQGQRGPP GETGPKGDRG IQGSRGIPGS PGPKGDTGLP GVDGRDGIPG
560 570 580 590 600
MPGTKGEAGK PGPPGDVGLQ GLPGVPGIPG AKGVAGEKGN TGAPGKPGQL
610 620 630 640 650
GSSGKPGQQG PPGEVGPRGP RGLPGSRGPV GPEGSPGIPG KLGSVGSPGL
660 670 680 690 700
PGLPGPPGLP GMKGDRGVFG EPGPKGEQGA SGEEGEAGAR GDLGDMGQPG
710 720 730 740 750
PKGSVGNPGE PGLRGPEGIR GLPGVEGPRG PPGPRGMQGE QGATGLPGIQ
760 770 780 790 800
GPPGRAPTDQ HIKQVCMRVV QEHFVEMAAS LKRPDTGASG LPGRPGPPGP
810 820 830 840 850
PGPPGENGFP GQMGIRGLPG IKGPPGALGL RGPKGDLGEK GERGPPGRGP
860 870 880 890 900
KGLPGAIGLP GDPGPASYGK NGRDGEQGPP GVAGIPGVPG PPGPPGPPGF
910 920
CEPASCTLQS GQRAFSKGPD K
Length:921
Mass (Da):92,092
Last modified:July 15, 1999 - v2
Checksum:iBC79177D36DCFFFC
GO
Isoform Short (identifier: Q05722-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-260: Missing.
     261-261: T → MAWAAWGRGVLGLSLMLSGLRLCAAQT

Show »
Length:687
Mass (Da):65,493
Checksum:i9F20E93756155B19
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101E → R in AAA21834. (PubMed:8061915)Curated
Sequence conflicti238 – 2381M → V in AAA21834. (PubMed:8061915)Curated
Sequence conflicti289 – 2891D → G in AAA21834. (PubMed:8061915)Curated
Sequence conflicti461 – 4611A → D in AAA21834. (PubMed:8061915)Curated
Sequence conflicti516 – 5161K → E in AAA21834. (PubMed:8061915)Curated
Sequence conflicti569 – 5702LQ → IA in AAA21834. (PubMed:8061915)Curated
Sequence conflicti740 – 7401E → D in AAA21834. (PubMed:8061915)Curated
Sequence conflicti775 – 7751V → A in AAA21834. (PubMed:8061915)Curated
Sequence conflicti775 – 7751V → A in CAA41049. (PubMed:2054384)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 260260Missing in isoform Short. CuratedVSP_001143Add
BLAST
Alternative sequencei261 – 2611T → MAWAAWGRGVLGLSLMLSGL RLCAAQT in isoform Short. CuratedVSP_001144

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17511 mRNA. Translation: BAA04463.1.
L12215 mRNA. Translation: AAA21834.1.
X57984 Genomic DNA. Translation: CAA41049.1.
M32136, M32132 Genomic DNA. Translation: AAA53523.1.
M32136, M32134 Genomic DNA. Translation: AAA53522.1.
CCDSiCCDS35527.1. [Q05722-1]
PIRiA35980.
S40495.
S42617.
RefSeqiNP_031766.3. NM_007740.3.
UniGeneiMm.154662.

Genome annotation databases

GeneIDi12839.
KEGGimmu:12839.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17511 mRNA. Translation: BAA04463.1 .
L12215 mRNA. Translation: AAA21834.1 .
X57984 Genomic DNA. Translation: CAA41049.1 .
M32136 , M32132 Genomic DNA. Translation: AAA53523.1 .
M32136 , M32134 Genomic DNA. Translation: AAA53522.1 .
CCDSi CCDS35527.1. [Q05722-1 ]
PIRi A35980.
S40495.
S42617.
RefSeqi NP_031766.3. NM_007740.3.
UniGenei Mm.154662.

3D structure databases

ProteinModelPortali Q05722.
SMRi Q05722. Positions 42-256.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q05722.

Proteomic databases

PaxDbi Q05722.
PRIDEi Q05722.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 12839.
KEGGi mmu:12839.

Organism-specific databases

CTDi 1297.
MGIi MGI:88465. Col9a1.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000085653.
HOVERGENi HBG004933.
InParanoidi Q05722.
KOi K08131.
PhylomeDBi Q05722.

Miscellaneous databases

NextBioi 282364.
PROi Q05722.
SOURCEi Search...

Gene expression databases

CleanExi MM_COL9A1.
Genevestigatori Q05722.

Family and domain databases

InterProi IPR008160. Collagen.
IPR013320. ConA-like_dom.
IPR001791. Laminin_G.
[Graphical view ]
Pfami PF01391. Collagen. 9 hits.
[Graphical view ]
SMARTi SM00210. TSPN. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete primary structure of the long form of mouse alpha 1(IX) collagen chain and its expression during limb development."
    Abe N., Yoshioka H., Inoue H., Ninomiya Y.
    Biochim. Biophys. Acta 1204:61-67(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
  2. "Assembly and sequencing of a cDNA covering the entire mouse alpha 1(IX) collagen chain."
    Rokos I., Muragaki Y., Warman M., Olsen B.R.
    Matrix Biol. 14:1-8(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Tissue: Embryo.
  3. "Specific hybridization probes for mouse type I, II, III and IX collagen mRNAs."
    Metsaeranta M., Toman D., de Crombrugghe B., Vuorio E.
    Biochim. Biophys. Acta 1089:241-243(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 759-906.
  4. "The alpha 1 (IX) collagen gene gives rise to two different transcripts in both mouse embryonic and human fetal RNA."
    Muragaki Y., Nishimura I., Henney A., Ninomiya Y., Olsen B.R.
    Proc. Natl. Acad. Sci. U.S.A. 87:2400-2404(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
    Strain: BALB/c.
    Tissue: Liver and Spleen.

Entry informationi

Entry nameiCO9A1_MOUSE
AccessioniPrimary (citable) accession number: Q05722
Secondary accession number(s): Q61269
, Q61270, Q61433, Q61940
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3