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Q05683

- DCE2_RAT

UniProt

Q05683 - DCE2_RAT

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Protein

Glutamate decarboxylase 2

Gene
Gad2, Gad65
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Pyridoxal phosphate.

Kineticsi

  1. KM=1.02 mM for glutamate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581Substrate By similarity

GO - Molecular functioni

  1. glutamate binding Source: RGD
  2. glutamate decarboxylase activity Source: RGD
  3. protein heterodimerization activity Source: RGD
  4. pyridoxal phosphate binding Source: RGD

GO - Biological processi

  1. glutamate decarboxylation to succinate Source: RGD
  2. neurotransmitter biosynthetic process Source: UniProtKB-KW
  3. response to drug Source: RGD
  4. synaptic transmission Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Neurotransmitter biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_196234. GABA synthesis.
SABIO-RKQ05683.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 2 (EC:4.1.1.15)
Alternative name(s):
65 kDa glutamic acid decarboxylase
Short name:
GAD-65
Glutamate decarboxylase 65 kDa isoform
Gene namesi
Name:Gad2
Synonyms:Gad65
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 17

Organism-specific databases

RGDi2653. Gad2.

Subcellular locationi

Cytoplasmcytosol By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapsepresynaptic cell membrane; Lipid-anchor By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity
Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters By similarity.1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: RGD
  2. axon Source: Ensembl
  3. cell junction Source: UniProtKB-KW
  4. cytoplasmic vesicle Source: RGD
  5. cytosol Source: RGD
  6. Golgi membrane Source: UniProtKB-SubCell
  7. perinuclear region of cytoplasm Source: RGD
  8. presynaptic membrane Source: UniProtKB-SubCell
  9. synaptic vesicle membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301C → A: Abolishes palmitoylation but not membrane-association; when associates with A-45. 1 Publication
Mutagenesisi45 – 451C → A: Abolishes palmitoylation but not membrane-association; when associates with A-30. 1 Publication
Mutagenesisi73 – 731C → S: No effect on palmitoylation. 1 Publication
Mutagenesisi75 – 751C → S: No effect on palmitoylation. 1 Publication
Mutagenesisi80 – 801C → S: No effect on palmitoylation. 1 Publication
Mutagenesisi82 – 821C → S: No effect on palmitoylation. 1 Publication
Mutagenesisi101 – 1011C → S: No effect on palmitoylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Glutamate decarboxylase 2PRO_0000146971Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31Phosphoserine1 Publication
Modified residuei6 – 61Phosphoserine1 Publication
Modified residuei10 – 101Phosphoserine1 Publication
Modified residuei13 – 131Phosphoserine1 Publication
Lipidationi30 – 301S-palmitoyl cysteine2 Publications
Lipidationi45 – 451S-palmitoyl cysteine2 Publications
Modified residuei396 – 3961N6-(pyridoxal phosphate)lysine By similarity

Post-translational modificationi

The N-terminus is blocked.
Phosphorylated; which does not affect kinetic parameters or subcellular location.1 Publication
Palmitoylated; which is required for presynaptic clustering By similarity.2 Publications

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiQ05683.

PTM databases

PhosphoSiteiQ05683.

Expressioni

Gene expression databases

GenevestigatoriQ05683.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

BioGridi246551. 1 interaction.
IntActiQ05683. 1 interaction.
STRINGi10116.ENSRNOP00000024901.

Structurei

3D structure databases

ProteinModelPortaliQ05683.
SMRiQ05683. Positions 88-584.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1833Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0076.
GeneTreeiENSGT00730000110441.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiQ05683.
KOiK01580.
OMAiWRAKGTT.
OrthoDBiEOG7H1JM3.
PhylomeDBiQ05683.
TreeFamiTF314688.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05683-1 [UniParc]FASTAAdd to Basket

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MASPGSGFWS FGSEDGSGDP ENPGTARAWC QVAQKFTGGI GNKLCALLYG    50
DSEKPAESGG SVTSRAATRK VACTCDQKPC SCPKGDVNYA LLHATDLLPA 100
CEGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA 150
DQPQNLEEIL THCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA 200
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA 250
MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI 300
LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV 350
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LNGVERANSV TWNPHKMMGV 400
PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV 450
DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYNIIKNREG YEMVFDGKPQ 500
HTNVCFWFVP PSLRVLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL 550
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL 585
Length:585
Mass (Da):65,402
Last modified:February 1, 1996 - v1
Checksum:iC04040B7BA7B37D1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901G → V AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M72422 mRNA. Translation: AAA63488.1.
PIRiJH0423.
RefSeqiNP_036695.1. NM_012563.1.
UniGeneiRn.29951.

Genome annotation databases

EnsembliENSRNOT00000024901; ENSRNOP00000024901; ENSRNOG00000018200.
GeneIDi24380.
KEGGirno:24380.
UCSCiRGD:2653. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M72422 mRNA. Translation: AAA63488.1 .
PIRi JH0423.
RefSeqi NP_036695.1. NM_012563.1.
UniGenei Rn.29951.

3D structure databases

ProteinModelPortali Q05683.
SMRi Q05683. Positions 88-584.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246551. 1 interaction.
IntActi Q05683. 1 interaction.
STRINGi 10116.ENSRNOP00000024901.

Chemistry

BindingDBi Q05683.

PTM databases

PhosphoSitei Q05683.

Proteomic databases

PRIDEi Q05683.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000024901 ; ENSRNOP00000024901 ; ENSRNOG00000018200 .
GeneIDi 24380.
KEGGi rno:24380.
UCSCi RGD:2653. rat.

Organism-specific databases

CTDi 2572.
RGDi 2653. Gad2.

Phylogenomic databases

eggNOGi COG0076.
GeneTreei ENSGT00730000110441.
HOGENOMi HOG000005382.
HOVERGENi HBG004980.
InParanoidi Q05683.
KOi K01580.
OMAi WRAKGTT.
OrthoDBi EOG7H1JM3.
PhylomeDBi Q05683.
TreeFami TF314688.

Enzyme and pathway databases

Reactomei REACT_196234. GABA synthesis.
SABIO-RK Q05683.

Miscellaneous databases

NextBioi 603141.
PROi Q05683.

Gene expression databases

Genevestigatori Q05683.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view ]
Pfami PF00282. Pyridoxal_deC. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Two genes encode distinct glutamate decarboxylases."
    Erlander M.G., Tillakaratne N.J., Feldblum S., Patel N., Tobin A.J.
    Neuron 7:91-100(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hippocampus.
  2. "Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase."
    Chang Y.C., Gottlieb D.I.
    J. Neurosci. 8:2123-2130(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 190-219; 225-266 AND 524-558.
    Tissue: Brain.
  3. "Membrane anchoring of the autoantigen GAD65 to microvesicles in pancreatic beta-cells by palmitoylation in the NH2-terminal domain."
    Christgau S., Aanstoot H.-J., Schierbeck H., Begley K., Tullin S., Hejnaes K., Baekkeskov S.
    J. Cell Biol. 118:309-320(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PALMITOYLATION.
  4. "Amino acid residues 24-31 but not palmitoylation of cysteines 30 and 45 are required for membrane anchoring of glutamic acid decarboxylase, GAD65."
    Shi Y., Veit B., Baekkeskov S.
    J. Cell Biol. 124:927-934(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-30 AND CYS-45, MUTAGENESIS OF CYS-30; CYS-45; CYS-73; CYS-75; CYS-80; CYS-82 AND CYS-101.
  5. "Phosphorylation of serine residues 3, 6, 10, and 13 distinguishes membrane anchored from soluble glutamic acid decarboxylase 65 and is restricted to glutamic acid decarboxylase 65alpha."
    Namchuk M., Lindsay L., Turck C.W., Kanaani J., Baekkeskov S.
    J. Biol. Chem. 272:1548-1557(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-3; SER-6; SER-10 AND SER-13, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiDCE2_RAT
AccessioniPrimary (citable) accession number: Q05683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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