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Protein

Glutamate decarboxylase 2

Gene

Gad2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Kineticsi

  1. KM=1.02 mM for glutamate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei558SubstrateBy similarity1

    GO - Molecular functioni

    • glutamate binding Source: RGD
    • glutamate decarboxylase activity Source: RGD
    • protein heterodimerization activity Source: RGD
    • pyridoxal phosphate binding Source: RGD

    GO - Biological processi

    • chemical synaptic transmission Source: RGD
    • glutamate decarboxylation to succinate Source: RGD
    • neurotransmitter biosynthetic process Source: UniProtKB-KW
    • response to drug Source: RGD

    Keywordsi

    Molecular functionDecarboxylase, Lyase
    Biological processNeurotransmitter biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiR-RNO-888568 GABA synthesis
    R-RNO-888590 GABA synthesis, release, reuptake and degradation
    SABIO-RKiQ05683

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase 2 (EC:4.1.1.15)
    Alternative name(s):
    65 kDa glutamic acid decarboxylase
    Short name:
    GAD-65
    Glutamate decarboxylase 65 kDa isoform
    Gene namesi
    Name:Gad2
    Synonyms:Gad65
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 17

    Organism-specific databases

    RGDi2653 Gad2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi30C → A: Abolishes palmitoylation but not membrane-association; when associates with A-45. 1 Publication1
    Mutagenesisi45C → A: Abolishes palmitoylation but not membrane-association; when associates with A-30. 1 Publication1
    Mutagenesisi73C → S: No effect on palmitoylation. 1 Publication1
    Mutagenesisi75C → S: No effect on palmitoylation. 1 Publication1
    Mutagenesisi80C → S: No effect on palmitoylation. 1 Publication1
    Mutagenesisi82C → S: No effect on palmitoylation. 1 Publication1
    Mutagenesisi101C → S: No effect on palmitoylation. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001469711 – 585Glutamate decarboxylase 2Add BLAST585

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei3PhosphoserineCombined sources1 Publication1
    Modified residuei6Phosphoserine1 Publication1
    Modified residuei10Phosphoserine1 Publication1
    Modified residuei13PhosphoserineCombined sources1 Publication1
    Lipidationi30S-palmitoyl cysteine1 Publication1
    Lipidationi45S-palmitoyl cysteine1 Publication1
    Modified residuei396N6-(pyridoxal phosphate)lysineBy similarity1

    Post-translational modificationi

    The N-terminus is blocked.
    Phosphorylated; which does not affect kinetic parameters or subcellular location.1 Publication
    Palmitoylated; which is required for presynaptic clustering.By similarity

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ05683
    PRIDEiQ05683

    PTM databases

    iPTMnetiQ05683
    PhosphoSitePlusiQ05683
    SwissPalmiQ05683

    Expressioni

    Gene expression databases

    BgeeiENSRNOG00000018200
    GenevisibleiQ05683 RN

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    GO - Molecular functioni

    • protein heterodimerization activity Source: RGD

    Protein-protein interaction databases

    BioGridi246551, 1 interactor
    IntActiQ05683, 1 interactor
    STRINGi10116.ENSRNOP00000024901

    Chemistry databases

    BindingDBiQ05683

    Structurei

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3CUPX-ray3.09B221-235[»]
    ProteinModelPortaliQ05683
    SMRiQ05683
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni181 – 183Substrate bindingBy similarity3

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiKOG0629 Eukaryota
    COG0076 LUCA
    GeneTreeiENSGT00760000119205
    HOGENOMiHOG000005382
    HOVERGENiHBG004980
    InParanoidiQ05683
    KOiK01580
    OMAiWRAKGTT
    OrthoDBiEOG091G07ZU
    PhylomeDBiQ05683
    TreeFamiTF314688

    Family and domain databases

    Gene3Di3.40.640.10, 1 hit
    InterProiView protein in InterPro
    IPR002129 PyrdxlP-dep_de-COase
    IPR015424 PyrdxlP-dep_Trfase
    IPR015421 PyrdxlP-dep_Trfase_major
    IPR021115 Pyridoxal-P_BS
    PfamiView protein in Pfam
    PF00282 Pyridoxal_deC, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    PROSITEiView protein in PROSITE
    PS00392 DDC_GAD_HDC_YDC, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q05683-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASPGSGFWS FGSEDGSGDP ENPGTARAWC QVAQKFTGGI GNKLCALLYG
    60 70 80 90 100
    DSEKPAESGG SVTSRAATRK VACTCDQKPC SCPKGDVNYA LLHATDLLPA
    110 120 130 140 150
    CEGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
    160 170 180 190 200
    DQPQNLEEIL THCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA
    210 220 230 240 250
    NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA
    260 270 280 290 300
    MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI
    310 320 330 340 350
    LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV
    360 370 380 390 400
    ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LNGVERANSV TWNPHKMMGV
    410 420 430 440 450
    PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV
    460 470 480 490 500
    DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYNIIKNREG YEMVFDGKPQ
    510 520 530 540 550
    HTNVCFWFVP PSLRVLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL
    560 570 580
    GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
    Length:585
    Mass (Da):65,402
    Last modified:February 1, 1996 - v1
    Checksum:iC04040B7BA7B37D1
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti190G → V AA sequence (PubMed:3385490).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M72422 mRNA Translation: AAA63488.1
    PIRiJH0423
    RefSeqiNP_036695.1, NM_012563.1
    UniGeneiRn.29951

    Genome annotation databases

    EnsembliENSRNOT00000024901; ENSRNOP00000024901; ENSRNOG00000018200
    GeneIDi24380
    KEGGirno:24380
    UCSCiRGD:2653 rat

    Similar proteinsi

    Entry informationi

    Entry nameiDCE2_RAT
    AccessioniPrimary (citable) accession number: Q05683
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: May 23, 2018
    This is version 129 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

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