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Q05681

- PP2B_NEUCR

UniProt

Q05681 - PP2B_NEUCR

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Protein
Serine/threonine-protein phosphatase 2B catalytic subunit
Gene
cna-1, 99H12.070, NCU03804
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 1 Fe3+ ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi128 – 1281Iron By similarity
Metal bindingi130 – 1301Iron By similarity
Metal bindingi156 – 1561Iron By similarity
Metal bindingi156 – 1561Zinc By similarity
Metal bindingi188 – 1881Zinc By similarity
Active sitei189 – 1891Proton donor By similarity
Metal bindingi237 – 2371Zinc By similarity
Metal bindingi319 – 3191Zinc By similarity

GO - Molecular functioni

  1. calcium-dependent protein serine/threonine phosphatase activity Source: EnsemblFungi
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: EnsemblFungi
  2. cellular ion homeostasis Source: EnsemblFungi
  3. fungal-type cell wall organization Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit (EC:3.1.3.16)
Alternative name(s):
Calmodulin-dependent calcineurin A subunit
Gene namesi
Name:cna-1
ORF Names:99H12.070, NCU03804
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 2, Linkage Group V

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: EnsemblFungi
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Serine/threonine-protein phosphatase 2B catalytic subunit
PRO_0000058833Add
BLAST

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Protein-protein interaction databases

STRINGi5141.NCU03804.1.

Structurei

3D structure databases

ProteinModelPortaliQ05681.
SMRiQ05681. Positions 46-410.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172699.
OrthoDBiEOG77M8X9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05681-1 [UniParc]FASTAAdd to Basket

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MESNNGTGAP GAFHTQQVDN AIRAIQHKRP LPEIDFTIHT MEDGSQVSTM    50
ERVCKDVQAP AMFKPSDEQF FEDETHTKPD IQFLKQHFYR EGRLTEEQAL 100
WIIREGTKLL RAEPNLLEMD APITVCGDVH GQYYDLMKLF EVGGDPAETR 150
YLFLGDYVDR GYFSIECVLY LWALKIHYPK TLWLLRGNHE CRHLTDYFTF 200
KLECKHKYSE AIYEACMESF CCLPLAAVMN KQFLCIHGGL SPELHTLDDI 250
RNIDRFREPP TQGLMCDILW ADPLEDFGQE KTTDFFVHNH VRGCSYFFSY 300
SAACHFLEKN NLLSIIRAHE AQDAGYRMYR KTRTTGFPSV MTIFSAPNYL 350
DVYNNKAAVL KYENNVMNIR QFNCTPHPYW LPNFMDVFTW SLPFVGEKIT 400
DMLIAILSTC SEEELREDSA TTSPGSASPA LPSAANQDPD SIEFKRRAIK 450
NKILAIGRLS RVFQVLREES ERVTELKTVS GGRLPAGTLM LGAEGIKNAI 500
SSFEDARKVD LQNERLPPSH DEVVKMQDEE RAQALERATR EADNDKKLQT 550
LSRRLSTS 558
Length:558
Mass (Da):63,914
Last modified:December 4, 2007 - v2
Checksum:iFDE74D72A000A55E
GO

Sequence cautioni

The sequence AAA33565.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAC18243.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73032 mRNA. Translation: AAA33565.1. Different initiation.
AL451018 Genomic DNA. Translation: CAC18243.1. Sequence problems.
CM002240 Genomic DNA. Translation: ESA42471.1.
PIRiA40942.

Genome annotation databases

EnsemblFungiiEFNCRT00000003478; EFNCRP00000003478; EFNCRG00000003474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73032 mRNA. Translation: AAA33565.1 . Different initiation.
AL451018 Genomic DNA. Translation: CAC18243.1 . Sequence problems.
CM002240 Genomic DNA. Translation: ESA42471.1 .
PIRi A40942.

3D structure databases

ProteinModelPortali Q05681.
SMRi Q05681. Positions 46-410.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU03804.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000003478 ; EFNCRP00000003478 ; EFNCRG00000003474 .

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172699.
OrthoDBi EOG77M8X9.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Calmodulin-dependent protein phosphatase from Neurospora crassa. Molecular cloning and expression of recombinant catalytic subunit."
    Higuchi S., Tamura J., Rathna Giri P., Polli J.W., Kincaid R.L.
    J. Biol. Chem. 266:18104-18112(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiPP2B_NEUCR
AccessioniPrimary (citable) accession number: Q05681
Secondary accession number(s): Q1K7G7, Q9HEE2, V5INZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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