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Q05681 (PP2B_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit
EC=3.1.3.16
Alternative name(s):
Calmodulin-dependent calcineurin A subunit
Gene names
Name:cna-1
ORF Names:99H12.070, NCU03804
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora ›

Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 Fe3+ ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

Sequence caution

The sequence AAA33565.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAC18243.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EAA31957.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 558558Serine/threonine-protein phosphatase 2B catalytic subunit
PRO_0000058833

Sites

Active site1891Proton donor By similarity
Metal binding1281Iron By similarity
Metal binding1301Iron By similarity
Metal binding1561Iron By similarity
Metal binding1561Zinc By similarity
Metal binding1881Zinc By similarity
Metal binding2371Zinc By similarity
Metal binding3191Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05681 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: FDE74D72A000A55E

FASTA55863,914
        10         20         30         40         50         60 
MESNNGTGAP GAFHTQQVDN AIRAIQHKRP LPEIDFTIHT MEDGSQVSTM ERVCKDVQAP 

        70         80         90        100        110        120 
AMFKPSDEQF FEDETHTKPD IQFLKQHFYR EGRLTEEQAL WIIREGTKLL RAEPNLLEMD 

       130        140        150        160        170        180 
APITVCGDVH GQYYDLMKLF EVGGDPAETR YLFLGDYVDR GYFSIECVLY LWALKIHYPK 

       190        200        210        220        230        240 
TLWLLRGNHE CRHLTDYFTF KLECKHKYSE AIYEACMESF CCLPLAAVMN KQFLCIHGGL 

       250        260        270        280        290        300 
SPELHTLDDI RNIDRFREPP TQGLMCDILW ADPLEDFGQE KTTDFFVHNH VRGCSYFFSY 

       310        320        330        340        350        360 
SAACHFLEKN NLLSIIRAHE AQDAGYRMYR KTRTTGFPSV MTIFSAPNYL DVYNNKAAVL 

       370        380        390        400        410        420 
KYENNVMNIR QFNCTPHPYW LPNFMDVFTW SLPFVGEKIT DMLIAILSTC SEEELREDSA 

       430        440        450        460        470        480 
TTSPGSASPA LPSAANQDPD SIEFKRRAIK NKILAIGRLS RVFQVLREES ERVTELKTVS 

       490        500        510        520        530        540 
GGRLPAGTLM LGAEGIKNAI SSFEDARKVD LQNERLPPSH DEVVKMQDEE RAQALERATR 

       550 
EADNDKKLQT LSRRLSTS

« Hide

References

« Hide 'large scale' references
[1]"Calmodulin-dependent protein phosphatase from Neurospora crassa. Molecular cloning and expression of recombinant catalytic subunit."
Higuchi S., Tamura J., Rathna Giri P., Polli J.W., Kincaid R.L.
J. Biol. Chem. 266:18104-18112(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M73032 mRNA. Translation: AAA33565.1. Different initiation.
AL451018 Genomic DNA. Translation: CAC18243.1. Sequence problems.
AABX02000011 Genomic DNA. Translation: EAA31957.2. Sequence problems.
PIRA40942.
RefSeqXP_961193.2. XM_956100.2.
UniGeneNcr.7826.

3D structure databases

ProteinModelPortalQ05681.
SMRQ05681. Positions 46-410.
ModBaseSearch...

Protein-protein interaction databases

STRING5141.NCU03804.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000003478; EFNCRP00000003478; EFNCRG00000003474.
GeneID3877353.
KEGGncr:NCU03804.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172699.
KOK04348.
OrthoDBEOG45XC4G.

Family and domain databases

InterProIPR004843. Metallo_PEstase_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP2B_NEUCR
AccessionPrimary (citable) accession number: Q05681
Secondary accession number(s): Q1K7G7, Q9HEE2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 4, 2007
Last modified: April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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