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Protein

RNA-binding suppressor of PAS kinase protein 1

Gene

RBS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

  • galactose metabolic process Source: SGD
  • RNA Polymerase III assembly Source: SGD
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29574-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding suppressor of PAS kinase protein 1
Gene namesi
Name:RBS1
Ordered Locus Names:YDL189W
ORF Names:D1266
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL189W.
SGDiS000002348. RBS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457RNA-binding suppressor of PAS kinase protein 1PRO_0000076298Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei198 – 1981PhosphoserineCombined sources
Modified residuei435 – 4351PhosphoserineCombined sources
Modified residuei439 – 4391PhosphoserineCombined sources
Modified residuei447 – 4471PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05672.
PeptideAtlasiQ05672.

PTM databases

iPTMnetiQ05672.

Interactioni

Protein-protein interaction databases

BioGridi31856. 24 interactions.
DIPiDIP-2980N.
IntActiQ05672. 11 interactions.
MINTiMINT-4479834.

Structurei

3D structure databases

ProteinModelPortaliQ05672.
SMRiQ05672. Positions 19-82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 8863R3HPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi234 – 2396Poly-Ser

Sequence similaritiesi

Contains 1 R3H domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ05672.
OrthoDBiEOG7FV407.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
InterProiIPR001374. R3H_dom.
[Graphical view]
PfamiPF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05672-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALDSRNWG LTPAMETGLF QKPQDRIFII ELENSIVSFI NSNTESFQLR
60 70 80 90 100
PMNSYYRLLS HQIAEYHNLN HVLARTQDSC VILFKGENFQ KIEGKPLLQE
110 120 130 140 150
LQLNKKPEEC ASSSESIEKS NNNRIFRILK RKEVGNECDY KIDGNTRTPN
160 170 180 190 200
SNLTANSNKD QKIEIDDKSS TDLEQERIEK ERLYEQRKQE IFDKLNKSED
210 220 230 240 250
DVKSTNSSGS SDSDNEWSDW LNGDDSNTQT SNGSVSSSSP FNSSVTTIQV
260 270 280 290 300
NKPQQQFYDS RRGRGGRRRG TNNYKDAYRG QSRRNKENGG YQSGYSSPYL
310 320 330 340 350
VYPPPQMGGN SLPTYPLMYN PAGPAPGPAP SPMVMGNNTV FMNPYMYNMN
360 370 380 390 400
PQGSCSFGTP IPMYPPYQYQ YQYQYNTQYH SGPYSNTPSY NSNNYTRSSA
410 420 430 440 450
NKYHHFQGKN SYSGAIPKRS DDSNSNKNEG IRRASVEGSP SSRDTDSVEM

KFDKLNI
Length:457
Mass (Da):51,957
Last modified:November 1, 1996 - v1
Checksum:iEDBF59E8F10B2001
GO

Sequence cautioni

The sequence CAA41660.1 differs from that shown. Reason: Frameshift at positions 294 and 322. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 33MTA → MRVSLSVSSFFHIQLDKFWR RQLSHIVPASRGDT in AAP04344 (PubMed:12844361).Curated
Sequence conflicti43 – 431N → S in AAP04344 (PubMed:12844361).Curated
Sequence conflicti81 – 811V → A in AAP04344 (PubMed:12844361).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA. Translation: CAA58258.1.
Z74237 Genomic DNA. Translation: CAA98766.1.
AY245794 mRNA. Translation: AAP04344.1.
X58857 Genomic DNA. Translation: CAA41660.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA11674.1.
PIRiS67744.
RefSeqiNP_010092.2. NM_001180249.1.

Genome annotation databases

EnsemblFungiiYDL189W; YDL189W; YDL189W.
GeneIDi851338.
KEGGisce:YDL189W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA. Translation: CAA58258.1.
Z74237 Genomic DNA. Translation: CAA98766.1.
AY245794 mRNA. Translation: AAP04344.1.
X58857 Genomic DNA. Translation: CAA41660.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA11674.1.
PIRiS67744.
RefSeqiNP_010092.2. NM_001180249.1.

3D structure databases

ProteinModelPortaliQ05672.
SMRiQ05672. Positions 19-82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31856. 24 interactions.
DIPiDIP-2980N.
IntActiQ05672. 11 interactions.
MINTiMINT-4479834.

PTM databases

iPTMnetiQ05672.

Proteomic databases

MaxQBiQ05672.
PeptideAtlasiQ05672.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL189W; YDL189W; YDL189W.
GeneIDi851338.
KEGGisce:YDL189W.

Organism-specific databases

EuPathDBiFungiDB:YDL189W.
SGDiS000002348. RBS1.

Phylogenomic databases

InParanoidiQ05672.
OrthoDBiEOG7FV407.

Enzyme and pathway databases

BioCyciYEAST:G3O-29574-MONOMER.

Miscellaneous databases

PROiQ05672.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
InterProiIPR001374. R3H_dom.
[Graphical view]
PfamiPF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae."
    Verhasselt P., Voet M., Volckaert G.
    Yeast 11:961-966(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
    Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
    Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-119.
    Strain: ATCC 204511 / S288c / AB972.
  5. "Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis."
    Ronne H., Carlberg M., Hu G.-Z., Nehlin J.O.
    Mol. Cell. Biol. 11:4876-4884(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 165-457.
    Strain: ATCC 208353 / W303-1A.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-435 AND SER-439, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198; SER-435 AND SER-439, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-447, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRBS1_YEAST
AccessioniPrimary (citable) accession number: Q05672
Secondary accession number(s): D6VRG4
, Q06896, Q07619, Q870H2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 892 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.