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Protein

Nuclear fusion protein FUS2

Gene

FUS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes cell fusion during zygote formation.

GO - Molecular functioni

  1. Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  1. conjugation with cellular fusion Source: SGD
  2. cytogamy Source: SGD
  3. karyogamy involved in conjugation with cellular fusion Source: SGD
  4. regulation of termination of mating projection growth Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32913-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear fusion protein FUS2
Gene namesi
Name:FUS2
Ordered Locus Names:YMR232W
ORF Names:YM9959.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR232w.
EuPathDBiFungiDB:YMR232W.
SGDiS000004845. FUS2.

Subcellular locationi

  1. Cell tip

  2. Note: Localizes at the mating projection tip (shmoo).

GO - Cellular componenti

  1. cell tip Source: UniProtKB-SubCell
  2. cytoplasm Source: SGD
  3. mating projection tip Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 677677Nuclear fusion protein FUS2PRO_0000080954Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201Phosphothreonine1 Publication
Modified residuei67 – 671Phosphoserine1 Publication
Modified residuei72 – 721Phosphoserine1 Publication
Modified residuei84 – 841Phosphoserine1 Publication
Modified residuei88 – 881Phosphothreonine1 Publication
Modified residuei100 – 1001Phosphoserine1 Publication
Modified residuei106 – 1061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ05670.

Expressioni

Gene expression databases

GenevestigatoriQ05670.

Interactioni

Protein-protein interaction databases

BioGridi35410. 45 interactions.
DIPiDIP-2711N.
IntActiQ05670. 12 interactions.
MINTiMINT-472882.

Structurei

3D structure databases

ProteinModelPortaliQ05670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 326215DHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG43014.
HOGENOMiHOG000112669.
InParanoidiQ05670.
OMAiLTPIRDY.
OrthoDBiEOG7BP8CX.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
InterProiIPR000219. DH-domain.
[Graphical view]
SMARTiSM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKTSYNLYD LNYPKNDSLT PIRDYKNDYF HKNDDKLPEI VRKPTRKLSK
60 70 80 90 100
HENKLNDKKF TNKRPASLDL HSIVESLSNK KIYSPINTEI FQNVVRLNLS
110 120 130 140 150
PQIPNSPHEG CKFYKIVQEF YLSEVEYYNN LLTANNVYRK ALNSDPRFKN
160 170 180 190 200
KLVKLDSSDE LLLFGNIDTI ASISKILVTA IKDLLLAKQR GKMLDANEWQ
210 220 230 240 250
KIFTKNEVQQ QLYSTFDISE AFEQHLLRIK STYTSYFVSH QKQMELFTTL
260 270 280 290 300
RMNKNHFFNK WYEYCLKESG CIKLEDILKS PMKRLTQWID TLETLESCYE
310 320 330 340 350
DILSPELGLK LSPTRRKYSL FSNKLETEVS EYKSNSMYNF SLTPSEIIQS
360 370 380 390 400
YDEDQFTHLL KPPDKQNKNI CNASRQESNL DNSRVPSLLS GSSSYYSDVS
410 420 430 440 450
GLEIVTNTST ASAEMINLKM DEETEFFTLA DHISKFKKVM KGLLELKKNL
460 470 480 490 500
LKNDLSGIID ISLRRINAWK KVIECERPSG AFFAHDNLIS TMCSSYIDKL
510 520 530 540 550
HEQKNQVTIL KLTELETDVM NPLERIIAHC TTVKSKLKDL QALKKDYMLF
560 570 580 590 600
LQEKKANVRD IKRDLLGMHF QNLQNQMKRE LPVFITLIHD TIECILLNYI
610 620 630 640 650
KVFLKYLEII AGGKKYLQKD LENMSLNDSI ATGQIKNLDI LQCYSKSRYM
660 670
TKRMVRKDWP FPGDPSGSRV VRKLFEL
Length:677
Mass (Da):79,037
Last modified:November 1, 1997 - v1
Checksum:iD7C89ED0738EA31F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851L → R in CAA62275 (PubMed:7559752).Curated
Sequence conflicti203 – 2031F → L in CAA62275 (PubMed:7559752).Curated
Sequence conflicti543 – 5464Missing in CAA62275 (PubMed:7559752).Curated
Sequence conflicti589 – 67789HDTIE…KLFEL → PRYYRMYLVELYQSLLKIFG NHCWWKKIPAKRS in CAA62275 (PubMed:7559752).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90752 Genomic DNA. Translation: CAA62275.1.
Z49939 Genomic DNA. Translation: CAA90203.1.
BK006946 Genomic DNA. Translation: DAA10132.1.
PIRiS57599.
RefSeqiNP_013959.1. NM_001182739.1.

Genome annotation databases

EnsemblFungiiYMR232W; YMR232W; YMR232W.
GeneIDi855272.
KEGGisce:YMR232W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90752 Genomic DNA. Translation: CAA62275.1.
Z49939 Genomic DNA. Translation: CAA90203.1.
BK006946 Genomic DNA. Translation: DAA10132.1.
PIRiS57599.
RefSeqiNP_013959.1. NM_001182739.1.

3D structure databases

ProteinModelPortaliQ05670.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35410. 45 interactions.
DIPiDIP-2711N.
IntActiQ05670. 12 interactions.
MINTiMINT-472882.

Proteomic databases

PaxDbiQ05670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR232W; YMR232W; YMR232W.
GeneIDi855272.
KEGGisce:YMR232W.

Organism-specific databases

CYGDiYMR232w.
EuPathDBiFungiDB:YMR232W.
SGDiS000004845. FUS2.

Phylogenomic databases

eggNOGiNOG43014.
HOGENOMiHOG000112669.
InParanoidiQ05670.
OMAiLTPIRDY.
OrthoDBiEOG7BP8CX.

Enzyme and pathway databases

BioCyciYEAST:G3O-32913-MONOMER.

Miscellaneous databases

NextBioi978888.
PROiQ05670.

Gene expression databases

GenevestigatoriQ05670.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
InterProiIPR000219. DH-domain.
[Graphical view]
SMARTiSM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation."
    Elion E.A., Trueheart J., Fink G.R.
    J. Cell Biol. 130:1283-1296(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-67; SER-72; SER-84; THR-88; SER-100 AND SER-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.

Entry informationi

Entry nameiFUS2_YEAST
AccessioniPrimary (citable) accession number: Q05670
Secondary accession number(s): D6W058, Q05023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 29, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.