Reviewed,
UniProtKB/Swiss-Prot Q05670 (FUS2_YEAST)
Last modified
December 15, 2009.
Version 70.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nuclear fusion protein FUS2 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 677 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Promotes cell fusion during zygote formation. |
| Subcellular location | Note: Localizes near the site of cell fusion. |
| Sequence similarities | Contains 1 DH (DBL-homology) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Fusion protein |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cytogamy Ref.1 Inferred from mutant phenotype. Source: SGD karyogamy during conjugation with cellular fusion Ref.1Inferred from mutant phenotype. Source: SGD regulation of Rho protein signal transductionInferred from electronic annotation. Source: InterPro regulation of termination of mating projection growthInferred from mutant phenotype. Source: SGD |
| Cellular component | mating projection tip Ref.1 Inferred from direct assay. Source: SGD mitochondrionInferred from direct assay. Source: SGD nucleusInferred from direct assay. Source: SGD |
| Molecular function | Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 677 | 677 | Nuclear fusion protein FUS2 | PRO_0000080954 | |||||
Regions | |||||||||
| Domain | 112 – 326 | 215 | DH | ||||||
Amino acid modifications | |||||||||
| Modified residue | 20 | 1 | Phosphothreonine Ref.3 | ||||||
| Modified residue | 67 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 72 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 84 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 88 | 1 | Phosphothreonine Ref.3 | ||||||
| Modified residue | 100 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 106 | 1 | Phosphoserine Ref.3 Ref.4 | ||||||
Experimental info | |||||||||
| Sequence conflict | 185 | 1 | L → R in CAA62275. Ref.1 | ||||||
| Sequence conflict | 203 | 1 | F → L in CAA62275. Ref.1 | ||||||
| Sequence conflict | 543 – 546 | 4 | Missing in CAA62275. Ref.1 | ||||||
| Sequence conflict | 589 – 677 | 89 | HDTIE…KLFEL → PRYYRMYLVELYQSLLKIFG NHCWWKKIPAKRS in CAA62275. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation." Elion E.A., Trueheart J., Fink G.R. J. Cell Biol. 130:1283-1296(1995) [PubMed: 7559752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G.Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-67; SER-72; SER-84; THR-88; SER-100 AND SER-106, MASS SPECTROMETRY. |
| [4] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X90752 Genomic DNA. Translation: CAA62275.1. Z49939 Genomic DNA. Translation: CAA90203.1. | |
| PIR | S57599. |
| RefSeq | NP_013959.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-2711N. |
| IntAct | Q05670. 3 interactions. |
| STRING | Q05670. |
Proteomic databases | |
| PRIDE | Q05670. |
Genome annotation databases | |
| Ensembl | YMR232W; YMR232W; YMR232W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 855272. |
| KEGG | sce:YMR232W. |
| NMPDR | fig|4932.3.peg.5013. |
Organism-specific databases | |
| CYGD | YMR232w. |
| SGD | S000004845. FUS2. |
Phylogenomic databases | |
| OMA | LTPIRDY. |
| OrthoDB | EOG95B2XN. |
Gene expression databases | |
| ArrayExpress | Q05670. |
| Genevestigator | Q05670. |
| GermOnline | YMR232W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR000219. DH-domain. [Graphical view] |
| Gene3D | G3DSA:1.20.900.10. RhoGEF. 1 hit. |
| SMART | SM00325. RhoGEF. 1 hit. [Graphical view] |
| PROSITE | PS00741. DH_1. False negative. PS50010. DH_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 978888. |
Entry information
| Entry name | FUS2_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q05670 Secondary accession number(s): Q05023 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIII Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names |

Clusters with


