Q05670 (FUS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear fusion protein FUS2

Gene names

Name:	FUS2
Ordered Locus Names:	YMR232W
ORF Names:	YM9959.14

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes cell fusion during zygote formation.
Subcellular location	Cell tip. Note: Localizes at the mating projection tip (shmoo).
Sequence similarities	Contains 1 DH (DBL-homology) domain.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytogamy Inferred from mutant phenotype PubMed 3302672Ref.1. Source: SGD karyogamy involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.1. Source: SGD regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of termination of mating projection growth Inferred from mutant phenotype PubMed 14734532. Source: SGD
Cellular_component	cell tip Inferred from electronic annotation. Source: UniProtKB-SubCell mating projection tip Inferred from direct assay Ref.1. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD nucleus Inferred from direct assay PubMed 18474625. Source: SGD
Molecular_function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	Nuclear fusion protein FUS2		PRO_0000080954
Regions
	Domain	112 – 326	215	DH
Amino acid modifications
	Modified residue	20	1	Phosphothreonine Ref.4
	Modified residue	67	1	Phosphoserine Ref.4
	Modified residue	72	1	Phosphoserine Ref.4
	Modified residue	84	1	Phosphoserine Ref.4
	Modified residue	88	1	Phosphothreonine Ref.4
	Modified residue	100	1	Phosphoserine Ref.4
	Modified residue	106	1	Phosphoserine Ref.4 Ref.5
Experimental info
	Sequence conflict	185	1	L → R in CAA62275. Ref.1
	Sequence conflict	203	1	F → L in CAA62275. Ref.1
	Sequence conflict	543 – 546	4	Missing in CAA62275. Ref.1
	Sequence conflict	589 – 677	89	HDTIE…KLFEL → PRYYRMYLVELYQSLLKIFG NHCWWKKIPAKRS in CAA62275. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q05670 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: D7C89ED0738EA31F	FASTA	677	79,037
	10 20 30 40 50 60 MFKTSYNLYD LNYPKNDSLT PIRDYKNDYF HKNDDKLPEI VRKPTRKLSK HENKLNDKKF 70 80 90 100 110 120 TNKRPASLDL HSIVESLSNK KIYSPINTEI FQNVVRLNLS PQIPNSPHEG CKFYKIVQEF 130 140 150 160 170 180 YLSEVEYYNN LLTANNVYRK ALNSDPRFKN KLVKLDSSDE LLLFGNIDTI ASISKILVTA 190 200 210 220 230 240 IKDLLLAKQR GKMLDANEWQ KIFTKNEVQQ QLYSTFDISE AFEQHLLRIK STYTSYFVSH 250 260 270 280 290 300 QKQMELFTTL RMNKNHFFNK WYEYCLKESG CIKLEDILKS PMKRLTQWID TLETLESCYE 310 320 330 340 350 360 DILSPELGLK LSPTRRKYSL FSNKLETEVS EYKSNSMYNF SLTPSEIIQS YDEDQFTHLL 370 380 390 400 410 420 KPPDKQNKNI CNASRQESNL DNSRVPSLLS GSSSYYSDVS GLEIVTNTST ASAEMINLKM 430 440 450 460 470 480 DEETEFFTLA DHISKFKKVM KGLLELKKNL LKNDLSGIID ISLRRINAWK KVIECERPSG 490 500 510 520 530 540 AFFAHDNLIS TMCSSYIDKL HEQKNQVTIL KLTELETDVM NPLERIIAHC TTVKSKLKDL 550 560 570 580 590 600 QALKKDYMLF LQEKKANVRD IKRDLLGMHF QNLQNQMKRE LPVFITLIHD TIECILLNYI 610 620 630 640 650 660 KVFLKYLEII AGGKKYLQKD LENMSLNDSI ATGQIKNLDI LQCYSKSRYM TKRMVRKDWP 670 FPGDPSGSRV VRKLFEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation." Elion E.A., Trueheart J., Fink G.R. J. Cell Biol. 130:1283-1296(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-67; SER-72; SER-84; THR-88; SER-100 AND SER-106, MASS SPECTROMETRY. Strain: ADR376.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X90752 Genomic DNA. Translation: CAA62275.1. Z49939 Genomic DNA. Translation: CAA90203.1. BK006946 Genomic DNA. Translation: DAA10132.1.
PIR	S57599.
RefSeq	NP_013959.1. NM_001182739.1.
3D structure databases
ProteinModelPortal	Q05670.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2711N.
IntAct	Q05670. 2 interactions.
MINT	MINT-472882.
Proteomic databases
PaxDb	Q05670.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR232W; YMR232W; YMR232W.
GeneID	855272.
KEGG	sce:YMR232W.
Organism-specific databases
CYGD	YMR232w.
SGD	S000004845. FUS2.
Phylogenomic databases
eggNOG	NOG43014.
HOGENOM	HOG000112669.
OMA	LTPIRDY.
OrthoDB	EOG43V34H.
Gene expression databases
Genevestigator	Q05670.
GermOnline	YMR232W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.20.900.10. 1 hit.
InterPro	IPR000219. DH-domain. [Graphical view]
SMART	SM00325. RhoGEF. 1 hit. [Graphical view]
SUPFAM	SSF48065. DH-domain. 1 hit.
PROSITE	PS00741. DH_1. False negative. PS50010. DH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978888.

Entry information

Entry name

FUS2_YEAST

Accession

Primary (citable) accession number: Q05670
Secondary accession number(s): D6W058, Q05023

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program