Reviewed,
UniProtKB/Swiss-Prot Q05639 (EF1A2_HUMAN)
Last modified
June 16, 2009.
Version 100.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Elongation factor 1-alpha 2 Short name=EF-1-alpha-2 Short name=Elongation factor 1 A-2 Alternative name(s): eEF1A-2 Statin S1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 463 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. |
| Subunit structure | Monomer By similarity. |
| Subcellular location | Nucleus By similarity. |
| Tissue specificity | Brain, heart, and skeletal muscle. |
| Sequence similarities | Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Nucleus |
| Ligand | GTP-binding Nucleotide-binding |
| Molecular function | Elongation factor |
| PTM | Methylation Phosphoprotein |
| Technical term | 3D-structure |
| Gene Ontology (GO) | |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activityInferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct translation elongation factor activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| EEF1B2 | P24534 | 1 | EBI-354943,EBI-354334 | |
| EEF1D | P29692 | 1 | EBI-354943,EBI-358607 | |
| VARS | P26640 | 1 | EBI-354943,EBI-355765 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 463 | 463 | Elongation factor 1-alpha 2 | PRO_0000090891 | |||||
Regions | |||||||||
| Nucleotide binding | 14 – 21 | 8 | GTP By similarity | ||||||
| Nucleotide binding | 91 – 95 | 5 | GTP By similarity | ||||||
| Nucleotide binding | 153 – 156 | 4 | GTP By similarity | ||||||
Sites | |||||||||
| Binding site | 301 | 1 | Glycerylphosphorylethanolamine (covalent) By similarity | ||||||
| Binding site | 374 | 1 | Glycerylphosphorylethanolamine (covalent) By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 29 | 1 | Phosphotyrosine Ref.7 Ref.8 | ||||||
| Modified residue | 55 | 1 | N6,N6,N6-trimethyllysine By similarity | ||||||
| Modified residue | 141 | 1 | Phosphotyrosine Ref.7 Ref.8 | ||||||
| Modified residue | 165 | 1 | N6,N6,N6-trimethyllysine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 427 | 1 | R → P in AAA91835. Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Tissue-dependent variation in the expression of elongation factor-1 alpha isoforms: isolation and characterisation of a cDNA encoding a novel variant of human elongation-factor 1 alpha." Knudsen S.M., Frydenberg J., Clark B.F.C., Leffers H. Eur. J. Biochem. 215:549-554(1993) [PubMed: 8354261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The human elongation factor 1 A-2 gene (EEF1A2): complete sequence and characterization of gene structure and promoter activity." Bischoff C., Kahns S., Lund A., Joergensen H.F., Praestegaard M., Clark B.F.C., Leffers H. Genomics 68:63-70(2000) [PubMed: 10950927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. Nomura N.Nat. Methods 5:1011-1017(2008) [PubMed: 19054851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. Rogers J.Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Duodenum and Lung. |
| [6] | "Cloning of human and mouse brain cDNAs coding for S1, the second member of the mammalian elongation factor-1 alpha gene family: analysis of a possible evolutionary pathway." Lee S., Ann D.K., Wang E. Biochem. Biophys. Res. Commun. 203:1371-1377(1994) [PubMed: 7945283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-463. Tissue: Hippocampus. |
| [7] | "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-29 AND TYR-141, MASS SPECTROMETRY. |
| [8] | "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. Comb M.J.Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-29 AND TYR-141, MASS SPECTROMETRY. Tissue: Lung. |
| [9] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X70940 mRNA. Translation: CAA50280.1. AF163763 Genomic DNA. Translation: AAF80488.1. AB451389 mRNA. Translation: BAG70203.1. AL121829 Genomic DNA. Translation: CAC15522.1. BC000432 mRNA. Translation: AAH00432.1. BC110409 mRNA. Translation: AAI10410.1. L10340 mRNA. Translation: AAA91835.1. | |||||||||||||
| IPI | IPI00014424. | ||||||||||||
| PIR | EFHUA2. S35033. | ||||||||||||
| RefSeq | NP_001949.1. | ||||||||||||
| UniGene | Hs.370895 Hs.433839 | ||||||||||||
3D structure databases | |||||||||||||
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| SMR | Q05639. Positions 2-443. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | Q05639. 20 interactions. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q05639. | ||||||||||||
Proteomic databases | |||||||||||||
| PeptideAtlas | Q05639. | ||||||||||||
| PRIDE | Q05639. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000101210. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 1917. | ||||||||||||
| KEGG | hsa:1917. | ||||||||||||
| NMPDR | fig|9606.3.peg.20674. | ||||||||||||
Organism-specific databases | |||||||||||||
| GeneCards | GC20M061589. | ||||||||||||
| H-InvDB | HIX0022989. | ||||||||||||
| HGNC | HGNC:3192. EEF1A2. | ||||||||||||
| MIM | 602959. gene. | ||||||||||||
| PharmGKB | PA27628. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | Q05639. | ||||||||||||
| HOVERGEN | Q05639. | ||||||||||||
| OMA | Q05639. EIPQLGR. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q05639. | ||||||||||||
| Bgee | Q05639. | ||||||||||||
| CleanEx | HS_EEF1A2. | ||||||||||||
| GermOnline | ENSG00000101210. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR000795. ProtSyn_GTP_bd. IPR004539. Transl_elong_EF1A_euk/arc. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004160. Transl_elong_EFTu/EF1A_C. [Graphical view] | ||||||||||||
| Pfam | PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR00315. ELONGATNFCT. | ||||||||||||
| TIGRFAMs | TIGR00483. EF-1_alpha. 1 hit. | ||||||||||||
| PROSITE | PS00301. EFACTOR_GTP. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 7803. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | EF1A2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q05639 Secondary accession number(s): B5BUF3, P54266, Q0VGC7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 20 Human chromosome 20: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


