Reviewed, UniProtKB/Swiss-Prot Q05638 (CHIX_STROI)

Names and origin

Protein names

Recommended name:
    Exochitinase 1
    EC=3.2.1.14

Gene names

Name:

chi01

Organism

Streptomyces olivaceoviridis (Streptomyces corchorusii)

Taxonomic identifier

1921 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exochitinase that generates exclusively chitobiose from chitotetraose, chitohexaose, and colloidal high-molecular mass chitin.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Enzyme regulation	Inhibited by the pseudosugar allosamidin A.
Induction	By chitin.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. Contains 1 fibronectin type-III domain.
biophysicochemical properties	pH dependence: Optimum pH is 7.3. Temperature dependence: Optimum temperature is 55 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Or 32 Potential
	Chain	30 – 597	568	Exochitinase 1		PRO_0000011910
Regions
	Domain	169 – 250	82	Fibronectin type-III
	Region	260 – 597	338	Catalytic
Sites
	Active site	384	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05638-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 7F5D615DD5EBC1F4	FASTA	597	62,314
	10 20 30 40 50 60 MDRFRPLAVL IAAALTLSGT TALSSAARAA DADLARNGGF EAGLDGWTCT AGTTVNSPVR 70 80 90 100 110 120 SGSSALKATP AGSDNARCAQ TVTVQPNSQY TLSGHVQGSY VYLGASGTGT TDVSTWTQSA 130 140 150 160 170 180 PDWRQLTTTF RTGPSTTRVT LYTHGWYGTG AYHADDISLV GPGGGTEQPP APPTGLRTGS 190 200 210 220 230 240 VTATSVALSW SPVTGATGYA VYRDGVKVAT ASGTSATVTG LTPDTAYAFQ VAAVNGAGES 250 260 270 280 290 300 AKSATVTATT APGTGGGSAD LPPHALVGYL HASFANGSGY TRLADVPDSW DVIDLAFGEP 310 320 330 340 350 360 TSVTSGDIRF DRCPATECPN VESDAEFKAA IKAKQAAGKK VLISIGGQNG QVQLTTTAAR 370 380 390 400 410 420 DTFVSSVSKI IDEYGLDGLD IDFEGHSLSL NADDTDFKNP KTPVIVNLIQ ALKTLKAKYG 430 440 450 460 470 480 DDFVLTMAPE TFFVQLGYQY YGTGKWGGQD PRAGAYLPVI HALRDDLTLL HVQDYNSGPI 490 500 510 520 530 540 MGLDNQYHSM GGADFHIAMT DMLLTGFPVA GDAANVFPPL RADQVAIGMP ATTNAGNGHV 550 560 570 580 590 APAEAVKTLD CLTRKTNCGS YATHGTWPAL RALMTWSINW DRFGGWEFQR TFDGYFG

References

[1]

"Characteristics of an exochitinase from Streptomyces olivaceoviridis, its corresponding gene, putative protein domains and relationship to other chitinases." Blaak H., Schnellmann J., Walter S., Henrissat B., Schrempf H. Eur. J. Biochem. 214:659-669(1993) [PubMed: 8319677] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11238 / DSM 41433 / NCIB 8592 / QM B814.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X71080 Genomic DNA. Translation: CAA50398.1.
PIR	S32039. S33848.
3D structure databases
HSSP	HSSP built from PDB template 1K85 based on UniProtKB P20533.
ModBase	Search...
Protein family/group databases
CAZy	CBM16. Carbohydrate-Binding Module Family 16. GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 119250.
Family and domain databases
InterPro	IPR003305. CenC_carb_bd. IPR011583. Chitinase_II. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02018. CBM_4_9. 1 hit. PF00041. fn3. 1 hit. PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PRINTS	PR00014. FNTYPEIII.
SMART	SM00060. FN3. 1 hit. SM00636. Glyco_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. PS50853. FN3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIX_STROI

Accession

Primary (citable) accession number: Q05638

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)