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Protein

Exochitinase 1

Gene

chi01

Organism
Streptomyces olivaceoviridis (Streptomyces corchorusii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exochitinase that generates exclusively chitobiose from chitotetraose, chitohexaose, and colloidal high-molecular mass chitin.

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Enzyme regulationi

Inhibited by the pseudosugar allosamidin A.

pH dependencei

Optimum pH is 7.3.

Temperature dependencei

Optimum temperature is 55 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei384Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM16. Carbohydrate-Binding Module Family 16.
GH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Exochitinase 1 (EC:3.2.1.14)
Gene namesi
Name:chi01
OrganismiStreptomyces olivaceoviridis (Streptomyces corchorusii)
Taxonomic identifieri1921 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Or 32Sequence analysisAdd BLAST29
ChainiPRO_000001191030 – 597Exochitinase 1Add BLAST568

Post-translational modificationi

The N-terminus is blocked.

Expressioni

Inductioni

By chitin.

Structurei

3D structure databases

ProteinModelPortaliQ05638.
SMRiQ05638.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 253Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni260 – 597CatalyticAdd BLAST338

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR003305. CenC_carb-bd.
IPR011583. Chitinase_II.
IPR003961. FN3_dom.
IPR036116. FN3_sf.
IPR008979. Galactose-bd-like_sf.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
PfamiView protein in Pfam
PF02018. CBM_4_9. 1 hit.
PF00041. fn3. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
SMARTiView protein in SMART
SM00060. FN3. 1 hit.
SM00636. Glyco_18. 1 hit.
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS01095. CHITINASE_18. 1 hit.
PS50853. FN3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRFRPLAVL IAAALTLSGT TALSSAARAA DADLARNGGF EAGLDGWTCT
60 70 80 90 100
AGTTVNSPVR SGSSALKATP AGSDNARCAQ TVTVQPNSQY TLSGHVQGSY
110 120 130 140 150
VYLGASGTGT TDVSTWTQSA PDWRQLTTTF RTGPSTTRVT LYTHGWYGTG
160 170 180 190 200
AYHADDISLV GPGGGTEQPP APPTGLRTGS VTATSVALSW SPVTGATGYA
210 220 230 240 250
VYRDGVKVAT ASGTSATVTG LTPDTAYAFQ VAAVNGAGES AKSATVTATT
260 270 280 290 300
APGTGGGSAD LPPHALVGYL HASFANGSGY TRLADVPDSW DVIDLAFGEP
310 320 330 340 350
TSVTSGDIRF DRCPATECPN VESDAEFKAA IKAKQAAGKK VLISIGGQNG
360 370 380 390 400
QVQLTTTAAR DTFVSSVSKI IDEYGLDGLD IDFEGHSLSL NADDTDFKNP
410 420 430 440 450
KTPVIVNLIQ ALKTLKAKYG DDFVLTMAPE TFFVQLGYQY YGTGKWGGQD
460 470 480 490 500
PRAGAYLPVI HALRDDLTLL HVQDYNSGPI MGLDNQYHSM GGADFHIAMT
510 520 530 540 550
DMLLTGFPVA GDAANVFPPL RADQVAIGMP ATTNAGNGHV APAEAVKTLD
560 570 580 590
CLTRKTNCGS YATHGTWPAL RALMTWSINW DRFGGWEFQR TFDGYFG
Length:597
Mass (Da):62,314
Last modified:June 1, 1994 - v1
Checksum:i7F5D615DD5EBC1F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71080 Genomic DNA. Translation: CAA50398.1.
PIRiS33848. S32039.

Similar proteinsi

Entry informationi

Entry nameiCHIX_STROI
AccessioniPrimary (citable) accession number: Q05638
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 22, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families