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Protein

Exosome complex component RRP45

Gene

RRP45

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP45 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation.2 Publications

Miscellaneous

Present with 4800 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processrRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29845-MONOMER.
ReactomeiR-SCE-429958. mRNA decay by 3' to 5' exoribonuclease.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Exosome complex component RRP45
Alternative name(s):
Ribosomal RNA-processing protein 45
Gene namesi
Name:RRP45
Ordered Locus Names:YDR280W
ORF Names:D9954.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR280W.
SGDiS000002688. RRP45.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Exosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001399741 – 305Exosome complex component RRP45Add BLAST305

Proteomic databases

MaxQBiQ05636.
PRIDEiQ05636.

Interactioni

Subunit structurei

Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which associates with catalytic subunits DIS3 and RRP6 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits and peripheral S1 domain-containing components CSL4, RRP4 and RRP40 located on the top of the ring structure. Interacts with LRP1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32333. 100 interactors.
DIPiDIP-2084N.
IntActiQ05636. 28 interactors.
MINTiMINT-555570.
STRINGi4932.YDR280W.

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 20ND:12
Beta strandi36 – 40ND:5
Beta strandi46 – 51ND:6
Beta strandi54 – 65ND:12
Beta strandi69 – 71ND:3
Beta strandi76 – 80ND:5
Helixi85 – 87ND:3
Helixi99 – 112ND:14
Turni113 – 116ND:4
Beta strandi123 – 126ND:4
Turni127 – 129ND:3
Beta strandi130 – 142ND:13
Helixi147 – 161ND:15
Beta strandi167 – 170ND:4
Beta strandi173 – 176ND:4
Turni179 – 181ND:3
Beta strandi194 – 201ND:8
Helixi206 – 211ND:6
Beta strandi218 – 222ND:5
Helixi225 – 228ND:4
Beta strandi232 – 239ND:8
Beta strandi243 – 257ND:15
Helixi258 – 298ND:41
Beta strandi299 – 302ND:4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WP8X-ray3.00A1-305[»]
4IFDX-ray2.80A2-305[»]
4OO1X-ray3.30A1-305[»]
5C0WX-ray4.60A1-305[»]
5C0XX-ray3.81A1-305[»]
5G06electron microscopy4.20A1-305[»]
5JEAX-ray2.65A1-305[»]
5K36X-ray3.10A1-305[»]
5OKZX-ray3.20A/K/U/e1-305[»]
5VZJX-ray3.30A1-305[»]
ProteinModelPortaliQ05636.
SMRiQ05636.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05636.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.IKR:

Phylogenomic databases

GeneTreeiENSGT00530000063093.
HOGENOMiHOG000229504.
InParanoidiQ05636.
KOiK03678.
OMAiMFMNSNL.
OrthoDBiEOG092C46HA.

Family and domain databases

CDDicd11368. RNase_PH_RRP45. 1 hit.
Gene3Di3.30.230.70. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR036345. ExoRNase_PH_dom2_sf.
IPR027408. PNPase/RNase_PH_dom_sf.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR033100. Rrp45.
PfamiView protein in Pfam
PF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
SUPFAMiSSF54211. SSF54211. 2 hits.
SSF55666. SSF55666. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKDIEISAS ESKFILEALR QNYRLDGRSF DQFRDVEITF GKEFGDVSVK
60 70 80 90 100
MGNTKVHCRI SCQIAQPYED RPFEGLFVIS TEISPMAGSQ FENGNITGED
110 120 130 140 150
EVLCSRIIEK SVRRSGALDV EGLCIVAGSK CWAVRADVHF LDCDGGFIDA
160 170 180 190 200
SCIAVMAGLM HFKKPDITVH GEQIIVHPVN EREPVPLGIL HIPICVTFSF
210 220 230 240 250
FNPQDTEENI KGETNSEISI IDATLKEELL RDGVLTVTLN KNREVVQVSK
260 270 280 290 300
AGGLPMDALT LMKCCHEAYS IIEKITDQIL QLLKEDSEKR NKYAAMLTSE

NAREI
Length:305
Mass (Da):33,962
Last modified:November 1, 1996 - v1
Checksum:i65C9DB5A63678F2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51030 Genomic DNA. Translation: AAB64446.1.
AY557748 Genomic DNA. Translation: AAS56074.1.
BK006938 Genomic DNA. Translation: DAA12120.1.
PIRiS70136.
RefSeqiNP_010566.1. NM_001180588.1.

Genome annotation databases

EnsemblFungiiYDR280W; YDR280W; YDR280W.
GeneIDi851874.
KEGGisce:YDR280W.

Similar proteinsi

Entry informationi

Entry nameiRRP45_YEAST
AccessioniPrimary (citable) accession number: Q05636
Secondary accession number(s): D6VSR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

According to PubMed:17173052 and PubMed:17174896, only DIS3/RRP44 subunit of the exosome core has exonuclease activity.IKR:

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names