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Protein

Ribonuclease H2 subunit B

Gene

RNH202

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.1 Publication

Miscellaneous

Present with 2340 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • DNA replication, removal of RNA primer Source: SGD
  • RNA catabolic process Source: SGD
  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC

Enzyme and pathway databases

BioCyciYEAST:G3O-29844-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H2 subunit B
Short name:
RNase H2 subunit B
Short name:
Rnh2B
Alternative name(s):
RNase H(202)
Ribonuclease HI subunit B
Gene namesi
Name:RNH202
Ordered Locus Names:YDR279W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR279W.
SGDiS000002687. RNH202.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483831 – 350Ribonuclease H2 subunit BAdd BLAST350

Proteomic databases

MaxQBiQ05635.
PRIDEiQ05635.

PTM databases

iPTMnetiQ05635.

Interactioni

Subunit structurei

The RNase 2 complex is a heterotrimer composed of the catalytic subunit RNH201 and of the non-catalytic subunits RNH202 and RNH203.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32332. 103 interactors.
DIPiDIP-1838N.
IntActiQ05635. 8 interactors.
MINTiMINT-399185.
STRINGi4932.YDR279W.

Structurei

3D structure databases

ProteinModelPortaliQ05635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000001049.
InParanoidiQ05635.
OMAiEAGDKYY.
OrthoDBiEOG092C576V.

Family and domain databases

CDDicd09270. RNase_H2-B. 1 hit.
InterProiView protein in InterPro
IPR019024. RNase_H2_suB.
PfamiView protein in Pfam
PF09468. RNase_H2-Ydr279. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVSNIGGEE RLIILPDDYE TSKTINTFTL PPPSNITSKP RIELFENING
60 70 80 90 100
KLYEIRSFQF GKGPSYSHEE DLANDKYHYT KENHPIKSTF IVNTSDPTDG
110 120 130 140 150
YVFNSSKIHF CSLYDIAFSL IGFYYRNSVS ADEQDYSNSS DTGENQKSNS
160 170 180 190 200
KTNEKFLTVR DYHDFLTDNH DKNWENISLS RLKSGLAKVS ETIEEAGDVY
210 220 230 240 250
YKITSAMITQ FLLGKVSKIV ENFPPSIPTL KNAPTEIKQC YKVVMATNLL
260 270 280 290 300
VSLIPRAAYH NLLTFSPTMD SGCLNPDIKA SFIELENYET TNELQNAERE
310 320 330 340 350
LLMKSAMNVG LNSNGRVSLP VKKVTKKIVQ NKKPKVAIGK GAIDGFFKRK
Length:350
Mass (Da):39,484
Last modified:November 1, 1996 - v1
Checksum:i68578B3018579583
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83N → S in AAS56115 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51030 Genomic DNA. Translation: AAB64447.1.
AY557789 Genomic DNA. Translation: AAS56115.1.
BK006938 Genomic DNA. Translation: DAA12119.1.
PIRiS70135.
RefSeqiNP_010565.3. NM_001180587.3.

Genome annotation databases

EnsemblFungiiYDR279W; YDR279W; YDR279W.
GeneIDi851873.
KEGGisce:YDR279W.

Similar proteinsi

Entry informationi

Entry nameiRNH2B_YEAST
AccessioniPrimary (citable) accession number: Q05635
Secondary accession number(s): D6VSQ9, Q6Q5R1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names