Q05622 (GUNE_RUMFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase E
Short name=EGE
EC=3.2.1.4

Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase E

Gene names

Name:

celE

Organism

Ruminococcus flavefaciens

Taxonomic identifier

1265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › Ruminococcus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Function	Hydrolyzes both carboxymethylcellulose and xylan. Probably has a role in hydrolyzing oligosaccharides derived from cellulose, which are transported across the cell wall.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 320	291	Endoglucanase E		PRO_0000012253
Sites
	Active site	150	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05622 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: DD5C549423405AFD	FASTA	320	35,938
	10 20 30 40 50 60 MRKRITSMIT VISLTLCGTI GTYPHNANAS AKEAFEDISK RSECTKVFDN DNGTYTAYSN 70 80 90 100 110 120 TAPIHYLNND EWKEIDNTLI EDSDDYYRNK DNSFNIYIPK EYSLGKNIKN PVIMNYDTFS 130 140 150 160 170 180 LSTSITDIKM PDRPEEETFS EGVYADINNE VYTTIDNSAI AEGMKAALKK SASMATYHSI 190 200 210 220 230 240 VEDIDLDIAV HNASVSESII INKLESLPET ITYSISVEDA IIKKTEDNRL QLIKDNEAVL 250 260 270 280 290 300 ILSPFTISDS SENVNVMQVE YDLTESEEGY EVTLYPAETV NRMSSPVMPL SLGSEYSYDR 310 320 PFSTVYNSQA SLHPSIIIIT

References

[1]	"Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1." Wang W., Reid S.J., Thomson J.A. J. Gen. Microbiol. 139:1219-1226(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FD-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03800 Genomic DNA. Translation: AAA26468.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

GUNE_RUMFL

Accession

Primary (citable) accession number: Q05622

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program