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Q05621 (DHPS_CLOB8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydropteroate synthase

Short name=DHPS
EC=2.5.1.15
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene names
Ordered Locus Names:Cbei_0206
OrganismClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP]
Taxonomic identifier290402 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.

Catalytic activity

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactor

Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.

Sequence similarities

Belongs to the DHPS family.

Contains 1 pterin-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Dihydropteroate synthase
PRO_0000168206

Regions

Domain14 – 261248Pterin-binding
Region61 – 622Substrate binding By similarity

Sites

Metal binding211Magnesium By similarity
Binding site291Substrate By similarity
Binding site951Substrate By similarity
Binding site1141Substrate By similarity
Binding site1781Substrate By similarity
Binding site2141Substrate By similarity
Binding site2491Substrate By similarity
Binding site2511Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05621 [UniParc].

Last modified February 26, 2008. Version 2.
Checksum: B66922317A603123

FASTA26929,948
        10         20         30         40         50         60 
MKIGSKEFNI GERTYIMGIL NFTPDSFSDG GKFNDIDVAV KHVKEMIDNG ADIIDVGGES 

        70         80         90        100        110        120 
TRPGYEIVSE EEEISRVVPI IKAIKEDFDI PVSIDTYKAK VAEQAIEAGA NLINDIWGFK 

       130        140        150        160        170        180 
KDKDMAKVAA KYNVPCCLMH NRDNTEYKNL MEDILNDLKE CINIAKDAGV KDENIILDPG 

       190        200        210        220        230        240 
IGFGKTYEQN LEAMNNLERI KELGYPILLG TSRKSMIGLA LNLPVEERIE GTVATTVIGI 

       250        260 
MKDACDFVRV HDVLENSRAA KMTDIIVRR 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of Clostridium beijerinckii NCIMB 8052."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Tapia R., Brainard J., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Mikhailova N., Bennet G., Cann I., Chen J.-S., Contreras A.L., Jones D., Kashket E., Mitchell W., Stoddard S., Schwarz W., Qureshi N., Young M., Shi Z., Ezeji T., White B., Blaschek H., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51743 / NCIMB 8052.
[2]"Cloning and sequence analysis of the genes encoding phosphotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052."
Oultram J.D., Burr I.D., Elmore M.J., Minton N.P.
Gene 131:107-112(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-205.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000721 Genomic DNA. Translation: ABR32396.1.
L04468 Genomic DNA. Translation: AAA52083.1.
RefSeqYP_001307352.1. NC_009617.1.

3D structure databases

ProteinModelPortalQ05621.
SMRQ05621. Positions 1-267.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290402.Cbei_0206.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR32396; ABR32396; Cbei_0206.
GeneID5291440.
KEGGcbe:Cbei_0206.
PATRIC19344252. VBICloBei69853_0211.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0294.
HOGENOMHOG000217509.
KOK00796.
OMADMFYVAA.
OrthoDBEOG67T5P5.

Enzyme and pathway databases

BioCycCBEI290402:GHL5-237-MONOMER.
UniPathwayUPA00077; UER00156.

Family and domain databases

Gene3D3.20.20.20. 1 hit.
InterProIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMSSF51717. SSF51717. 1 hit.
TIGRFAMsTIGR01496. DHPS. 1 hit.
PROSITEPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHPS_CLOB8
AccessionPrimary (citable) accession number: Q05621
Secondary accession number(s): A6LPW6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 26, 2008
Last modified: May 14, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways