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Protein

Dihydropteroate synthase

Gene

Cbei_0206

Organism
Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.By similarity

Catalytic activityi

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Dihydropteroate synthase (Cbei_0206)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi21MagnesiumBy similarity1
Binding sitei616-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei956-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1146-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1786-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei2146-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Folate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase (EC:2.5.1.15)
Short name:
DHPS
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene namesi
Ordered Locus Names:Cbei_0206
OrganismiClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Taxonomic identifieri290402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000565 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001682061 – 269Dihydropteroate synthaseAdd BLAST269

Interactioni

Protein-protein interaction databases

STRINGi290402.Cbei_0206.

Structurei

3D structure databases

ProteinModelPortaliQ05621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 261Pterin-bindingPROSITE-ProRule annotationAdd BLAST248

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni249 – 2516-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the DHPS family.Curated
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105EEI. Bacteria.
COG0294. LUCA.
HOGENOMiHOG000217509.
KOiK00796.
OMAiSIDTYHA.
OrthoDBiPOG091H00BJ.

Family and domain databases

CDDicd00739. DHPS. 1 hit.
Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding_dom.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGSKEFNI GERTYIMGIL NFTPDSFSDG GKFNDIDVAV KHVKEMIDNG
60 70 80 90 100
ADIIDVGGES TRPGYEIVSE EEEISRVVPI IKAIKEDFDI PVSIDTYKAK
110 120 130 140 150
VAEQAIEAGA NLINDIWGFK KDKDMAKVAA KYNVPCCLMH NRDNTEYKNL
160 170 180 190 200
MEDILNDLKE CINIAKDAGV KDENIILDPG IGFGKTYEQN LEAMNNLERI
210 220 230 240 250
KELGYPILLG TSRKSMIGLA LNLPVEERIE GTVATTVIGI MKDACDFVRV
260
HDVLENSRAA KMTDIIVRR
Length:269
Mass (Da):29,948
Last modified:February 26, 2008 - v2
Checksum:iB66922317A603123
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000721 Genomic DNA. Translation: ABR32396.1.
L04468 Genomic DNA. Translation: AAA52083.1.
RefSeqiWP_011967558.1. NC_009617.1.

Genome annotation databases

EnsemblBacteriaiABR32396; ABR32396; Cbei_0206.
KEGGicbe:Cbei_0206.
PATRICi19344252. VBICloBei69853_0211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000721 Genomic DNA. Translation: ABR32396.1.
L04468 Genomic DNA. Translation: AAA52083.1.
RefSeqiWP_011967558.1. NC_009617.1.

3D structure databases

ProteinModelPortaliQ05621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290402.Cbei_0206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR32396; ABR32396; Cbei_0206.
KEGGicbe:Cbei_0206.
PATRICi19344252. VBICloBei69853_0211.

Phylogenomic databases

eggNOGiENOG4105EEI. Bacteria.
COG0294. LUCA.
HOGENOMiHOG000217509.
KOiK00796.
OMAiSIDTYHA.
OrthoDBiPOG091H00BJ.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.

Family and domain databases

CDDicd00739. DHPS. 1 hit.
Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding_dom.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHPS_CLOB8
AccessioniPrimary (citable) accession number: Q05621
Secondary accession number(s): A6LPW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 26, 2008
Last modified: November 30, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.