Q05609 (CTR1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase CTR1
EC=2.7.11.1

Gene names

Name:	CTR1
Ordered Locus Names:	At5g03730
ORF Names:	F17C15_150

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	821 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a negative regulator in the ethylene response pathway. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Expressed in both seedlings and adult plants. Ref.1
Disruption phenotype	Mutants display ethylene-treated phenotypes, resulting in plants with small, unexpanded leaves and whose seed cotyledon growth is impaired. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Ethylene signaling pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to iron ion Inferred from expression pattern PubMed 20627899. Source: TAIR ethylene mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW gibberellin biosynthetic process Inferred from mutant phenotype PubMed 17389366. Source: TAIR negative regulation of ethylene mediated signaling pathway Traceable author statement PubMed 12535337PubMed 12821658Ref.1PubMed 9560288. Source: TAIR protein autophosphorylation Inferred from direct assay PubMed 21477822. Source: TAIR regulation of post-embryonic root development Inferred from mutant phenotype PubMed 17656722. Source: TAIR regulation of stem cell division Inferred from mutant phenotype PubMed 17656722. Source: TAIR regulation of timing of transition from vegetative to reproductive phase Inferred from mutant phenotype PubMed 17389366. Source: TAIR response to ethylene stimulus Inferred from mutant phenotype Ref.1. Source: TAIR response to fructose stimulus Inferred from mutant phenotype PubMed 21253566. Source: TAIR response to sucrose stimulus Inferred from mutant phenotype PubMed 11162499. Source: TAIR sugar mediated signaling pathway Traceable author statement PubMed 12663220. Source: TAIR
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay PubMed 12821658. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay PubMed 12535337PubMed 21477822. Source: TAIR protein serine/threonine/tyrosine kinase activity Inferred from sequence or structural similarity PubMed 16429265. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERS1	Q38846	2	EBI-1606697,EBI-1606754
ETR1	P49333	6	EBI-1606697,EBI-1606682
ETR2	Q0WPQ2	2	EBI-1606697,EBI-1787533

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 821

821

Serine/threonine-protein kinase CTR1

PRO_0000085907

Regions

Domain

551 – 809

259

Protein kinase

Nucleotide binding

557 – 565

9

ATP By similarity

Compositional bias

65 – 69

5

Poly-Gly

Compositional bias

135 – 141

7

Poly-Gly

Sites

Active site

676

1

Proton acceptor By similarity

Binding site

578

1

ATP By similarity

Experimental info

Mutagenesis

596

1

E → K in ctr1-4; exhibits ethylene-treated phenotype.

Mutagenesis

694

1

D → E in ctr1-1; exhibits ethylene-treated phenotype.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

821

Helix Strand Turn

Details...

Helix

548 – 550

3

Beta strand

551 – 559

9

Beta strand

561 – 570

10

Beta strand

573 – 580

8

Helix

587 – 602

16

Beta strand

611 – 615

5

Beta strand

617 – 619

3

Beta strand

622 – 626

5

Helix

633 – 638

6

Helix

642 – 645

4

Helix

648 – 666

19

Beta strand

668 – 670

3

Helix

679 – 681

3

Beta strand

682 – 684

3

Beta strand

690 – 692

3

Beta strand

703 – 705

3

Turn

715 – 717

3

Helix

720 – 723

4

Helix

732 – 746

15

Turn

750 – 753

4

Helix

756 – 765

10

Helix

778 – 787

10

Helix

792 – 794

3

Helix

798 – 809

12

Sequences

	Sequence		Length	Mass (Da)
	Q05609 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2922D3DCD0CC15BC	FASTA	821	90,306
	10 20 30 40 50 60 MEMPGRRSNY TLLSQFSDDQ VSVSVTGAPP PHYDSLSSEN RSNHNSGNTG KAKAERGGFD 70 80 90 100 110 120 WDPSGGGGGD HRLNNQPNRV GNNMYASSLG LQRQSSGSSF GESSLSGDYY MPTLSAAANE 130 140 150 160 170 180 IESVGFPQDD GFRLGFGGGG GDLRIQMAAD SAGGSSSGKS WAQQTEESYQ LQLALALRLS 190 200 210 220 230 240 SEATCADDPN FLDPVPDESA LRTSPSSAET VSHRFWVNGC LSYYDKVPDG FYMMNGLDPY 250 260 270 280 290 300 IWTLCIDLHE SGRIPSIESL RAVDSGVDSS LEAIIVDRRS DPAFKELHNR VHDISCSCIT 310 320 330 340 350 360 TKEVVDQLAK LICNRMGGPV IMGEDELVPM WKECIDGLKE IFKVVVPIGS LSVGLCRHRA 370 380 390 400 410 420 LLFKVLADII DLPCRIAKGC KYCNRDDAAS CLVRFGLDRE YLVDLVGKPG HLWEPDSLLN 430 440 450 460 470 480 GPSSISISSP LRFPRPKPVE PAVDFRLLAK QYFSDSQSLN LVFDPASDDM GFSMFHRQYD 490 500 510 520 530 540 NPGGENDALA ENGGGSLPPS ANMPPQNMMR ASNQIEAAPM NAPPISQPVP NRANRELGLD 550 560 570 580 590 600 GDDMDIPWCD LNIKEKIGAG SFGTVHRAEW HGSDVAVKIL MEQDFHAERV NEFLREVAIM 610 620 630 640 650 660 KRLRHPNIVL FMGAVTQPPN LSIVTEYLSR GSLYRLLHKS GAREQLDERR RLSMAYDVAK 670 680 690 700 710 720 GMNYLHNRNP PIVHRDLKSP NLLVDKKYTV KVCDFGLSRL KASTFLSSKS AAGTPEWMAP 730 740 750 760 770 780 EVLRDEPSNE KSDVYSFGVI LWELATLQQP WGNLNPAQVV AAVGFKCKRL EIPRNLNPQV 790 800 810 820 AAIIEGCWTN EPWKRPSFAT IMDLLRPLIK SAVPPPNRSD L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases." Kieber J.J., Rothenberg M., Roman G., Feldmann K.A., Ecker J.R. Cell 72:427-441(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY. Strain: cv. Columbia. Tissue: Seedling.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L08789 mRNA. Translation: AAA32779.1.
L08790 Genomic DNA. Translation: AAA32780.1.
AL162506 Genomic DNA. Translation: CAB82938.1.
CP002688 Genomic DNA. Translation: AED90647.1.
CP002688 Genomic DNA. Translation: AED90648.1.

IPI

IPI00518290.

PIR

T48400.

RefSeq

NP_195993.1. NM_120454.3.
NP_850760.1. NM_180429.3.

UniGene

At.300.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3P86	X-ray	2.50	A/B	540-821	[»]
3PPZ	X-ray	2.99	A/B	540-821	[»]

ProteinModelPortal

Q05609.

SMR

Q05609. Positions 540-810.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q05609. 4 interactions.

STRING

3702.AT5G03730.1-P.

Proteomic databases

PaxDb

Q05609.

PRIDE

Q05609.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT5G03730.1; AT5G03730.1; AT5G03730.
AT5G03730.2; AT5G03730.2; AT5G03730.

GeneID

831748.

KEGG

ath:AT5G03730.

Organism-specific databases

GeneFarm

884.

TAIR

At5g03730.

Phylogenomic databases

eggNOG

COG0515.

HOGENOM

HOG000239752.

InParanoid

Q05609.

KO

K14510.

OMA

DKVPDGF.

PhylomeDB

Q05609.

ProtClustDB

CLSN2687572.

Enzyme and pathway databases

BRENDA

2.7.11.1. 399.

Gene expression databases

ArrayExpress

Q05609.

Genevestigator

Q05609.

GermOnline

AT5G03730. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR026840. ARMC3/CTR1.
IPR026839. CTR1.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

PANTHER

PTHR23257:SF80. PTHR23257:SF80. 1 hit.

Pfam

PF14381. EDR1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]

PRINTS

PR00109. TYRKINASE.

SMART

SM00220. S_TKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CTR1_ARATH

Accession

Primary (citable) accession number: Q05609

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program