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Q05608 (YPKA_YERPS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kinase YpkA

Short name=Protein kinase A
EC=2.7.11.1
Alternative name(s):
Targeted effector protein kinase
Gene names
Name:ypkA
Ordered Locus Names:pYV0001
Encoded onPlasmid pIB1 Ref.1 Ref.2
Plasmid pYV Ref.3
OrganismYersinia pseudotuberculosis serotype I (strain IP32953) [Complete proteome] [HAMAP]
Taxonomic identifier273123 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a virulence determinant. Ref.5 Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Secreted Ref.4.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processVirulence
   Cellular componentSecreted
   DomainSignal
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   Technical term3D-structure
Complete proteome
Plasmid
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – ? Potential
Chain? – 732Protein kinase YpkAPRO_0000024392

Regions

Domain136 – 408273Protein kinase
Nucleotide binding142 – 1509ATP By similarity

Sites

Active site2701Proton acceptor By similarity
Binding site1631ATP By similarity

Experimental info

Sequence conflict5661H → N Ref.1
Sequence conflict5661H → N Ref.2

Secondary structure

............................. 732
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q05608 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: CEF903AAFE2E57BC

FASTA73281,731
        10         20         30         40         50         60 
MKSVKIMGTM PPSISLAKAH ERISQHWQNP VGELNIGGKR YRIIDNQVLR LNPHSGFSLF 

        70         80         90        100        110        120 
REGVGKIFSG KMFNFSIARN LTDTLHAAQK TTSQELRSDI PNALSNLFGA KPQTELPLGW 

       130        140        150        160        170        180 
KGEPLSGAPD LEGMRVAETD KFAEGESHIS IIETKDKQRL VAKIERSIAE GHLFAELEAY 

       190        200        210        220        230        240 
KHIYKTAGKH PNLANVHGMA VVPYGNRKEE ALLMDEVDGW RCSDTLRTLA DSWKQGKINS 

       250        260        270        280        290        300 
EAYWGTIKFI AHRLLDVTNH LAKAGVVHND IKPGNVVFDR ASGEPVVIDL GLHSRSGEQP 

       310        320        330        340        350        360 
KGFTESFKAP ELGVGNLGAS EKSDVFLVVS TLLHCIEGFE KNPEIKPNQG LRFITSEPAH 

       370        380        390        400        410        420 
VMDENGYPIH RPGIAGVETA YTRFITDILG VSADSRPDSN EARLHEFLSD GTIDEESAKQ 

       430        440        450        460        470        480 
ILKDTLTGEM SPLSTDVRRI TPKKLRELSD LLRTHLSSAA TKQLDMGGVL SDLDTMLVAL 

       490        500        510        520        530        540 
DKAEREGGVD KDQLKSFNSL ILKTYRVIED YVKGREGDTK NSSTEVSPYH RSNFMLSIVE 

       550        560        570        580        590        600 
PSLQRIQKHL DQTHSFSDIG SLVRAHKHLE TLLEVLVTLS QQGQPVSSET YGFLNRLAEA 

       610        620        630        640        650        660 
KITLSQQLNT LQQQQESAKA QLSILINRSG SWADVARQSL QRFDSTRPVV KFGTEQYTAI 

       670        680        690        700        710        720 
HRQMMAAHAA ITLQEVSEFT DDMRNFTVDS IPLLIQLGRS SLMDEHLVEQ REKLRELTTI 

       730 
AERLNRLERE WM 

« Hide

References

« Hide 'large scale' references
[1]"A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant."
Galyov E.E., Haakansson S., Forsberg A., Wolf-Watz H.
Nature 361:730-732(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YPIII / Serotype O:3.
[2]"Characterization of the operon encoding the YpkA Ser/Thr protein kinase and the YopJ protein of Yersinia pseudotuberculosis."
Galyov E.E., Haakansson S., Wolf-Watz H.
J. Bacteriol. 176:4543-4548(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YPIII / Serotype O:3.
[3]"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis."
Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. expand/collapse author list , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E.
Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IP32953.
[4]"The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane."
Hakansson S., Galyov E.E., Rosqvist R., Wolf-Watz H.
Mol. Microbiol. 20:593-603(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"A distinctive role for the Yersinia protein kinase: actin binding, kinase activation, and cytoskeleton disruption."
Juris S.J., Rudolph A.E., Huddler D., Orth K., Dixon J.E.
Proc. Natl. Acad. Sci. U.S.A. 97:9431-9436(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Identification of a molecular target for the Yersinia protein kinase A."
Navarro L., Koller A., Nordfelth R., Wolf-Watz H., Taylor S., Dixon J.E.
Mol. Cell 26:465-477(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors."
Prehna G., Ivanov M.I., Bliska J.B., Stebbins C.E.
Cell 126:869-880(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 434-732.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69439 Genomic DNA. Translation: CAA49215.1.
L33833 Genomic DNA. Translation: AAA68487.1.
BX936399 Genomic DNA. Translation: CAF25344.1.
PIRS30060.
RefSeqYP_068414.1. NC_006153.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2H7OX-ray2.00A434-732[»]
2H7VX-ray2.60C/D434-732[»]
ProteinModelPortalQ05608.
SMRQ05608. Positions 439-732.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ05608. 1 interaction.
MINTMINT-7717103.
STRING273123.pYV0001.

PTM databases

PhosSiteP010483.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAF25344; CAF25344; pYV0001.
GeneID2952894.
KEGGyps:pYV0001.
PATRIC18637748. VBIYerPse22266_0001.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000219636.
OMAMRNFTAD.
OrthoDBEOG6NKQVS.
ProtClustDBCLSK862113.

Enzyme and pathway databases

BioCycYPSE273123:GI1M-4097-MONOMER.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR019093. Rac1-binding_domain.
IPR008271. Ser/Thr_kinase_AS.
IPR003547. Ser/thr_kinase_yersinia-type.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF09632. Rac1. 1 hit.
[Graphical view]
PRINTSPR01373. YERSSTKINASE.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ05608.

Entry information

Entry nameYPKA_YERPS
AccessionPrimary (citable) accession number: Q05608
Secondary accession number(s): Q663G6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 23, 2004
Last modified: February 19, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references