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Q05608

- YPKA_YERPS

UniProt

Q05608 - YPKA_YERPS

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Protein

Protein kinase YpkA

Gene

ypkA

Organism
Yersinia pseudotuberculosis serotype I (strain IP32953)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a virulence determinant.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631ATPPROSITE-ProRule annotation
Active sitei270 – 2701Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi142 – 1509ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Virulence

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPSE273123:GI1M-4097-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase YpkA (EC:2.7.11.1)
Short name:
Protein kinase A
Alternative name(s):
Targeted effector protein kinase
Gene namesi
Name:ypkA
Ordered Locus Names:pYV0001
Encoded oniPlasmid pIB12 Publications
Plasmid pYV1 Publication
OrganismiYersinia pseudotuberculosis serotype I (strain IP32953)
Taxonomic identifieri273123 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000001011: Plasmid pYV

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 732Protein kinase YpkAPRO_0000024392
Signal peptidei1 – ?Sequence Analysis

PTM databases

PhosSiteiP010483.

Interactioni

Protein-protein interaction databases

IntActiQ05608. 1 interaction.
MINTiMINT-7717103.
STRINGi273123.pYV0001.

Structurei

Secondary structure

1
732
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi442 – 46019
Helixi466 – 48520
Helixi491 – 51323
Helixi534 – 5374
Helixi539 – 5479
Helixi548 – 5514
Helixi556 – 5583
Helixi559 – 58022
Beta strandi583 – 5853
Helixi588 – 62841
Helixi629 – 6313
Helixi633 – 64614
Helixi656 – 6583
Helixi659 – 67618
Helixi677 – 6793
Helixi680 – 69718
Helixi705 – 73026

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H7OX-ray2.00A434-732[»]
2H7VX-ray2.60C/D434-732[»]
ProteinModelPortaliQ05608.
SMRiQ05608. Positions 439-732.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05608.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini136 – 408273Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000219636.
OMAiMRNFTAD.
OrthoDBiEOG6NKQVS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR019093. Rac1-binding_domain.
IPR008271. Ser/Thr_kinase_AS.
IPR003547. Ser/thr_kinase_yersinia-type.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF09632. Rac1. 1 hit.
[Graphical view]
PRINTSiPR01373. YERSSTKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05608-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSVKIMGTM PPSISLAKAH ERISQHWQNP VGELNIGGKR YRIIDNQVLR
60 70 80 90 100
LNPHSGFSLF REGVGKIFSG KMFNFSIARN LTDTLHAAQK TTSQELRSDI
110 120 130 140 150
PNALSNLFGA KPQTELPLGW KGEPLSGAPD LEGMRVAETD KFAEGESHIS
160 170 180 190 200
IIETKDKQRL VAKIERSIAE GHLFAELEAY KHIYKTAGKH PNLANVHGMA
210 220 230 240 250
VVPYGNRKEE ALLMDEVDGW RCSDTLRTLA DSWKQGKINS EAYWGTIKFI
260 270 280 290 300
AHRLLDVTNH LAKAGVVHND IKPGNVVFDR ASGEPVVIDL GLHSRSGEQP
310 320 330 340 350
KGFTESFKAP ELGVGNLGAS EKSDVFLVVS TLLHCIEGFE KNPEIKPNQG
360 370 380 390 400
LRFITSEPAH VMDENGYPIH RPGIAGVETA YTRFITDILG VSADSRPDSN
410 420 430 440 450
EARLHEFLSD GTIDEESAKQ ILKDTLTGEM SPLSTDVRRI TPKKLRELSD
460 470 480 490 500
LLRTHLSSAA TKQLDMGGVL SDLDTMLVAL DKAEREGGVD KDQLKSFNSL
510 520 530 540 550
ILKTYRVIED YVKGREGDTK NSSTEVSPYH RSNFMLSIVE PSLQRIQKHL
560 570 580 590 600
DQTHSFSDIG SLVRAHKHLE TLLEVLVTLS QQGQPVSSET YGFLNRLAEA
610 620 630 640 650
KITLSQQLNT LQQQQESAKA QLSILINRSG SWADVARQSL QRFDSTRPVV
660 670 680 690 700
KFGTEQYTAI HRQMMAAHAA ITLQEVSEFT DDMRNFTVDS IPLLIQLGRS
710 720 730
SLMDEHLVEQ REKLRELTTI AERLNRLERE WM
Length:732
Mass (Da):81,731
Last modified:November 23, 2004 - v2
Checksum:iCEF903AAFE2E57BC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti566 – 5661H → N(PubMed:8441468)Curated
Sequence conflicti566 – 5661H → N(PubMed:8045884)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69439 Genomic DNA. Translation: CAA49215.1.
L33833 Genomic DNA. Translation: AAA68487.1.
BX936399 Genomic DNA. Translation: CAF25344.1.
PIRiS30060.
RefSeqiYP_068414.1. NC_006153.2.

Genome annotation databases

EnsemblBacteriaiCAF25344; CAF25344; pYV0001.
GeneIDi2952894.
KEGGiyps:pYV0001.
PATRICi18637748. VBIYerPse22266_0001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69439 Genomic DNA. Translation: CAA49215.1 .
L33833 Genomic DNA. Translation: AAA68487.1 .
BX936399 Genomic DNA. Translation: CAF25344.1 .
PIRi S30060.
RefSeqi YP_068414.1. NC_006153.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2H7O X-ray 2.00 A 434-732 [» ]
2H7V X-ray 2.60 C/D 434-732 [» ]
ProteinModelPortali Q05608.
SMRi Q05608. Positions 439-732.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q05608. 1 interaction.
MINTi MINT-7717103.
STRINGi 273123.pYV0001.

PTM databases

PhosSitei P010483.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAF25344 ; CAF25344 ; pYV0001 .
GeneIDi 2952894.
KEGGi yps:pYV0001.
PATRICi 18637748. VBIYerPse22266_0001.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000219636.
OMAi MRNFTAD.
OrthoDBi EOG6NKQVS.

Enzyme and pathway databases

BioCyci YPSE273123:GI1M-4097-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q05608.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR019093. Rac1-binding_domain.
IPR008271. Ser/Thr_kinase_AS.
IPR003547. Ser/thr_kinase_yersinia-type.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF09632. Rac1. 1 hit.
[Graphical view ]
PRINTSi PR01373. YERSSTKINASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant."
    Galyov E.E., Haakansson S., Forsberg A., Wolf-Watz H.
    Nature 361:730-732(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YPIII / Serotype O:3.
    Plasmid: pIB1
  2. "Characterization of the operon encoding the YpkA Ser/Thr protein kinase and the YopJ protein of Yersinia pseudotuberculosis."
    Galyov E.E., Haakansson S., Wolf-Watz H.
    J. Bacteriol. 176:4543-4548(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YPIII / Serotype O:3.
    Plasmid: pIB1
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IP32953.
    Plasmid: pYV
  4. "The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane."
    Hakansson S., Galyov E.E., Rosqvist R., Wolf-Watz H.
    Mol. Microbiol. 20:593-603(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "A distinctive role for the Yersinia protein kinase: actin binding, kinase activation, and cytoskeleton disruption."
    Juris S.J., Rudolph A.E., Huddler D., Orth K., Dixon J.E.
    Proc. Natl. Acad. Sci. U.S.A. 97:9431-9436(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Identification of a molecular target for the Yersinia protein kinase A."
    Navarro L., Koller A., Nordfelth R., Wolf-Watz H., Taylor S., Dixon J.E.
    Mol. Cell 26:465-477(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors."
    Prehna G., Ivanov M.I., Bliska J.B., Stebbins C.E.
    Cell 126:869-880(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 434-732.

Entry informationi

Entry nameiYPKA_YERPS
AccessioniPrimary (citable) accession number: Q05608
Secondary accession number(s): Q663G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 23, 2004
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3