Protein kinase ypkA precursor - Yersinia pseudotuberculosis

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Q05608 (YPKA_YERPS) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein kinase ypkA
Short name=Protein kinase A
EC=2.7.11.1

Alternative name(s):
Targeted effector protein kinase

Gene names

Name:	ypkA
Ordered Locus Names:	pYV0001

Encoded on

Plasmid pIB1 Ref.1 Ref.2
Plasmid pYV Ref.3

Organism

Yersinia pseudotuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

633 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a virulence determinant. Ref.5 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Secreted Ref.4.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	3D-structure Complete proteome Plasmid
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – ?

Potential

Chain

? – 732

Protein kinase ypkA

PRO_0000024392

Regions

Domain

136 – 408

273

Protein kinase

Nucleotide binding

142 – 150

ATP By similarity

Sites

Active site

270

Proton acceptor By similarity

Binding site

163

ATP By similarity

Experimental info

Sequence conflict

566

H → N Ref.1

Sequence conflict

566

H → N Ref.2

Secondary structure

732

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q05608 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: CEF903AAFE2E57BC
Show »

FASTA

732

81,731

        10         20         30         40         50         60 
MKSVKIMGTM PPSISLAKAH ERISQHWQNP VGELNIGGKR YRIIDNQVLR LNPHSGFSLF 

        70         80         90        100        110        120 
REGVGKIFSG KMFNFSIARN LTDTLHAAQK TTSQELRSDI PNALSNLFGA KPQTELPLGW 

       130        140        150        160        170        180 
KGEPLSGAPD LEGMRVAETD KFAEGESHIS IIETKDKQRL VAKIERSIAE GHLFAELEAY 

       190        200        210        220        230        240 
KHIYKTAGKH PNLANVHGMA VVPYGNRKEE ALLMDEVDGW RCSDTLRTLA DSWKQGKINS 

       250        260        270        280        290        300 
EAYWGTIKFI AHRLLDVTNH LAKAGVVHND IKPGNVVFDR ASGEPVVIDL GLHSRSGEQP 

       310        320        330        340        350        360 
KGFTESFKAP ELGVGNLGAS EKSDVFLVVS TLLHCIEGFE KNPEIKPNQG LRFITSEPAH 

       370        380        390        400        410        420 
VMDENGYPIH RPGIAGVETA YTRFITDILG VSADSRPDSN EARLHEFLSD GTIDEESAKQ 

       430        440        450        460        470        480 
ILKDTLTGEM SPLSTDVRRI TPKKLRELSD LLRTHLSSAA TKQLDMGGVL SDLDTMLVAL 

       490        500        510        520        530        540 
DKAEREGGVD KDQLKSFNSL ILKTYRVIED YVKGREGDTK NSSTEVSPYH RSNFMLSIVE 

       550        560        570        580        590        600 
PSLQRIQKHL DQTHSFSDIG SLVRAHKHLE TLLEVLVTLS QQGQPVSSET YGFLNRLAEA 

       610        620        630        640        650        660 
KITLSQQLNT LQQQQESAKA QLSILINRSG SWADVARQSL QRFDSTRPVV KFGTEQYTAI 

       670        680        690        700        710        720 
HRQMMAAHAA ITLQEVSEFT DDMRNFTVDS IPLLIQLGRS SLMDEHLVEQ REKLRELTTI 

       730 
AERLNRLERE WM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant." Galyov E.E., Haakansson S., Forsberg A., Wolf-Watz H. Nature 361:730-732(1993) [PubMed: 8441468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YPIII / Serotype O:3.
[2]	"Characterization of the operon encoding the YpkA Ser/Thr protein kinase and the YopJ protein of Yersinia pseudotuberculosis." Galyov E.E., Haakansson S., Wolf-Watz H. J. Bacteriol. 176:4543-4548(1994) [PubMed: 8045884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YPIII / Serotype O:3.
[3]	"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed: 15358858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953 / Serotype I.
[4]	"The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane." Hakansson S., Galyov E.E., Rosqvist R., Wolf-Watz H. Mol. Microbiol. 20:593-603(1996) [PubMed: 8736538] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"A distinctive role for the Yersinia protein kinase: actin binding, kinase activation, and cytoskeleton disruption." Juris S.J., Rudolph A.E., Huddler D., Orth K., Dixon J.E. Proc. Natl. Acad. Sci. U.S.A. 97:9431-9436(2000) [PubMed: 10920208] [Abstract] Cited for: FUNCTION.
[6]	"Identification of a molecular target for the Yersinia protein kinase A." Navarro L., Koller A., Nordfelth R., Wolf-Watz H., Taylor S., Dixon J.E. Mol. Cell 26:465-477(2007) [PubMed: 17531806] [Abstract] Cited for: FUNCTION.
[7]	"Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors." Prehna G., Ivanov M.I., Bliska J.B., Stebbins C.E. Cell 126:869-880(2006) [PubMed: 16959567] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 434-732.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X69439 Genomic DNA. Translation: CAA49215.1.
L33833 Genomic DNA. Translation: AAA68487.1.
BX936399 Genomic DNA. Translation: CAF25344.1.

PIR

S30060.

RefSeq

YP_068414.1. NC_006153.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H7O	X-ray	2.00	A	434-732	[»]
2H7V	X-ray	2.60	C/D	434-732	[»]

ProteinModelPortal

Q05608.

SMR

Q05608. Positions 439-732.

ModBase

Search...

PTM databases

PhosSite

Q05608.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2952894.

GenomeReviews

Gene locus pYV0001 in contig BX936399_GR.

KEGG

yps:pYV0001.

NMPDR

fig|273123.1.peg.1.

PATRIC

18637748. VBIYerPse22266_0001.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG299066.

OMA

VGELNIE.

ProtClustDB

CLSK862113.

Enzyme and pathway databases

BioCyc

YPSE273123:PYV0001-MONOMER.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR019093. Rac1-binding_domain.
IPR017442. Se/Thr_kinase-like_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR003547. Ser/thr_kinase_yersinia.
[Graphical view]

Pfam

PF00069. Pkinase. 1 hit.
PF09632. Rac1. 1 hit.
[Graphical view]

PRINTS

PR01373. YERSSTKINASE.

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

YPKA_YERPS

Accession

Primary (citable) accession number: Q05608
Secondary accession number(s): Q663G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents