Q05608 (YPKA_YERPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase YpkA
Short name=Protein kinase A
EC=2.7.11.1

Alternative name(s):
Targeted effector protein kinase

Gene names

Name:	ypkA
Ordered Locus Names:	pYV0001

Encoded on

Plasmid pIB1 Ref.1 Ref.2
Plasmid pYV Ref.3

Organism

Yersinia pseudotuberculosis serotype I (strain IP32953) [Complete proteome] [HAMAP]

Taxonomic identifier

273123 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a virulence determinant. Ref.5 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Secreted Ref.4.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	3D-structure Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – ?

Potential

Chain

? – 732

Protein kinase YpkA

PRO_0000024392

Regions

Domain

136 – 408

273

Protein kinase

Nucleotide binding

142 – 150

9

ATP By similarity

Sites

Active site

270

1

Proton acceptor By similarity

Binding site

163

1

ATP By similarity

Experimental info

Sequence conflict

566

1

H → N Ref.1

Sequence conflict

566

1

H → N Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

732

Helix Strand Turn

Details...

Helix

442 – 460

19

Helix

466 – 485

20

Helix

491 – 513

23

Helix

534 – 537

4

Helix

539 – 547

9

Helix

548 – 551

4

Helix

556 – 558

3

Helix

559 – 580

22

Beta strand

583 – 585

3

Helix

588 – 628

41

Helix

629 – 631

3

Helix

633 – 646

14

Helix

656 – 658

3

Helix

659 – 676

18

Helix

677 – 679

3

Helix

680 – 697

18

Helix

705 – 730

26

Sequences

	Sequence		Length	Mass (Da)
	Q05608 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: CEF903AAFE2E57BC	FASTA	732	81,731
	10 20 30 40 50 60 MKSVKIMGTM PPSISLAKAH ERISQHWQNP VGELNIGGKR YRIIDNQVLR LNPHSGFSLF 70 80 90 100 110 120 REGVGKIFSG KMFNFSIARN LTDTLHAAQK TTSQELRSDI PNALSNLFGA KPQTELPLGW 130 140 150 160 170 180 KGEPLSGAPD LEGMRVAETD KFAEGESHIS IIETKDKQRL VAKIERSIAE GHLFAELEAY 190 200 210 220 230 240 KHIYKTAGKH PNLANVHGMA VVPYGNRKEE ALLMDEVDGW RCSDTLRTLA DSWKQGKINS 250 260 270 280 290 300 EAYWGTIKFI AHRLLDVTNH LAKAGVVHND IKPGNVVFDR ASGEPVVIDL GLHSRSGEQP 310 320 330 340 350 360 KGFTESFKAP ELGVGNLGAS EKSDVFLVVS TLLHCIEGFE KNPEIKPNQG LRFITSEPAH 370 380 390 400 410 420 VMDENGYPIH RPGIAGVETA YTRFITDILG VSADSRPDSN EARLHEFLSD GTIDEESAKQ 430 440 450 460 470 480 ILKDTLTGEM SPLSTDVRRI TPKKLRELSD LLRTHLSSAA TKQLDMGGVL SDLDTMLVAL 490 500 510 520 530 540 DKAEREGGVD KDQLKSFNSL ILKTYRVIED YVKGREGDTK NSSTEVSPYH RSNFMLSIVE 550 560 570 580 590 600 PSLQRIQKHL DQTHSFSDIG SLVRAHKHLE TLLEVLVTLS QQGQPVSSET YGFLNRLAEA 610 620 630 640 650 660 KITLSQQLNT LQQQQESAKA QLSILINRSG SWADVARQSL QRFDSTRPVV KFGTEQYTAI 670 680 690 700 710 720 HRQMMAAHAA ITLQEVSEFT DDMRNFTVDS IPLLIQLGRS SLMDEHLVEQ REKLRELTTI 730 AERLNRLERE WM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant." Galyov E.E., Haakansson S., Forsberg A., Wolf-Watz H. Nature 361:730-732(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YPIII / Serotype O:3.
[2]	"Characterization of the operon encoding the YpkA Ser/Thr protein kinase and the YopJ protein of Yersinia pseudotuberculosis." Galyov E.E., Haakansson S., Wolf-Watz H. J. Bacteriol. 176:4543-4548(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YPIII / Serotype O:3.
[3]	"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953.
[4]	"The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane." Hakansson S., Galyov E.E., Rosqvist R., Wolf-Watz H. Mol. Microbiol. 20:593-603(1996) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"A distinctive role for the Yersinia protein kinase: actin binding, kinase activation, and cytoskeleton disruption." Juris S.J., Rudolph A.E., Huddler D., Orth K., Dixon J.E. Proc. Natl. Acad. Sci. U.S.A. 97:9431-9436(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Identification of a molecular target for the Yersinia protein kinase A." Navarro L., Koller A., Nordfelth R., Wolf-Watz H., Taylor S., Dixon J.E. Mol. Cell 26:465-477(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors." Prehna G., Ivanov M.I., Bliska J.B., Stebbins C.E. Cell 126:869-880(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 434-732.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X69439 Genomic DNA. Translation: CAA49215.1.
L33833 Genomic DNA. Translation: AAA68487.1.
BX936399 Genomic DNA. Translation: CAF25344.1.

PIR

S30060.

RefSeq

YP_068414.1. NC_006153.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H7O	X-ray	2.00	A	434-732	[»]
2H7V	X-ray	2.60	C/D	434-732	[»]

ProteinModelPortal

Q05608.

SMR

Q05608. Positions 439-732.

ModBase

Search...

Protein-protein interaction databases

STRING

273123.pYV0001.

PTM databases

PhosSite

P010483.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAF25344; CAF25344; pYV0001.

GeneID

2952894.

KEGG

yps:pYV0001.

PATRIC

18637748. VBIYerPse22266_0001.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0515.

HOGENOM

HOG000219636.

OMA

VGELNIE.

ProtClustDB

CLSK862113.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR019093. Rac1-binding_domain.
IPR008271. Ser/Thr_kinase_AS.
IPR003547. Ser/thr_kinase_yersinia-type.
[Graphical view]

Pfam

PF00069. Pkinase. 1 hit.
PF09632. Rac1. 1 hit.
[Graphical view]

PRINTS

PR01373. YERSSTKINASE.

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q05608.

Entry information

Entry name

YPKA_YERPS

Accession

Primary (citable) accession number: Q05608
Secondary accession number(s): Q663G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program