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Q05608

- YPKA_YERPS

UniProt

Q05608 - YPKA_YERPS

Protein

Protein kinase YpkA

Gene

ypkA

Organism
Yersinia pseudotuberculosis serotype I (strain IP32953)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Acts as a virulence determinant.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei163 – 1631ATPPROSITE-ProRule annotation
    Active sitei270 – 2701Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi142 – 1509ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Virulence

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYPSE273123:GI1M-4097-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase YpkA (EC:2.7.11.1)
    Short name:
    Protein kinase A
    Alternative name(s):
    Targeted effector protein kinase
    Gene namesi
    Name:ypkA
    Ordered Locus Names:pYV0001
    Encoded oniPlasmid pIB12 Publications
    Plasmid pYV1 Publication
    OrganismiYersinia pseudotuberculosis serotype I (strain IP32953)
    Taxonomic identifieri273123 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000001011: Plasmid pYV

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 732Protein kinase YpkAPRO_0000024392
    Signal peptidei1 – ?Sequence Analysis

    PTM databases

    PhosSiteiP010483.

    Interactioni

    Protein-protein interaction databases

    IntActiQ05608. 1 interaction.
    MINTiMINT-7717103.
    STRINGi273123.pYV0001.

    Structurei

    Secondary structure

    1
    732
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi442 – 46019
    Helixi466 – 48520
    Helixi491 – 51323
    Helixi534 – 5374
    Helixi539 – 5479
    Helixi548 – 5514
    Helixi556 – 5583
    Helixi559 – 58022
    Beta strandi583 – 5853
    Helixi588 – 62841
    Helixi629 – 6313
    Helixi633 – 64614
    Helixi656 – 6583
    Helixi659 – 67618
    Helixi677 – 6793
    Helixi680 – 69718
    Helixi705 – 73026

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2H7OX-ray2.00A434-732[»]
    2H7VX-ray2.60C/D434-732[»]
    ProteinModelPortaliQ05608.
    SMRiQ05608. Positions 439-732.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ05608.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini136 – 408273Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000219636.
    OMAiMRNFTAD.
    OrthoDBiEOG6NKQVS.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR019093. Rac1-binding_domain.
    IPR008271. Ser/Thr_kinase_AS.
    IPR003547. Ser/thr_kinase_yersinia-type.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF09632. Rac1. 1 hit.
    [Graphical view]
    PRINTSiPR01373. YERSSTKINASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05608-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSVKIMGTM PPSISLAKAH ERISQHWQNP VGELNIGGKR YRIIDNQVLR    50
    LNPHSGFSLF REGVGKIFSG KMFNFSIARN LTDTLHAAQK TTSQELRSDI 100
    PNALSNLFGA KPQTELPLGW KGEPLSGAPD LEGMRVAETD KFAEGESHIS 150
    IIETKDKQRL VAKIERSIAE GHLFAELEAY KHIYKTAGKH PNLANVHGMA 200
    VVPYGNRKEE ALLMDEVDGW RCSDTLRTLA DSWKQGKINS EAYWGTIKFI 250
    AHRLLDVTNH LAKAGVVHND IKPGNVVFDR ASGEPVVIDL GLHSRSGEQP 300
    KGFTESFKAP ELGVGNLGAS EKSDVFLVVS TLLHCIEGFE KNPEIKPNQG 350
    LRFITSEPAH VMDENGYPIH RPGIAGVETA YTRFITDILG VSADSRPDSN 400
    EARLHEFLSD GTIDEESAKQ ILKDTLTGEM SPLSTDVRRI TPKKLRELSD 450
    LLRTHLSSAA TKQLDMGGVL SDLDTMLVAL DKAEREGGVD KDQLKSFNSL 500
    ILKTYRVIED YVKGREGDTK NSSTEVSPYH RSNFMLSIVE PSLQRIQKHL 550
    DQTHSFSDIG SLVRAHKHLE TLLEVLVTLS QQGQPVSSET YGFLNRLAEA 600
    KITLSQQLNT LQQQQESAKA QLSILINRSG SWADVARQSL QRFDSTRPVV 650
    KFGTEQYTAI HRQMMAAHAA ITLQEVSEFT DDMRNFTVDS IPLLIQLGRS 700
    SLMDEHLVEQ REKLRELTTI AERLNRLERE WM 732
    Length:732
    Mass (Da):81,731
    Last modified:November 23, 2004 - v2
    Checksum:iCEF903AAFE2E57BC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti566 – 5661H → N(PubMed:8441468)Curated
    Sequence conflicti566 – 5661H → N(PubMed:8045884)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69439 Genomic DNA. Translation: CAA49215.1.
    L33833 Genomic DNA. Translation: AAA68487.1.
    BX936399 Genomic DNA. Translation: CAF25344.1.
    PIRiS30060.
    RefSeqiWP_011191359.1. NC_006153.2.
    YP_068414.1. NC_006153.2.

    Genome annotation databases

    EnsemblBacteriaiCAF25344; CAF25344; pYV0001.
    GeneIDi2952894.
    KEGGiyps:pYV0001.
    PATRICi18637748. VBIYerPse22266_0001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69439 Genomic DNA. Translation: CAA49215.1 .
    L33833 Genomic DNA. Translation: AAA68487.1 .
    BX936399 Genomic DNA. Translation: CAF25344.1 .
    PIRi S30060.
    RefSeqi WP_011191359.1. NC_006153.2.
    YP_068414.1. NC_006153.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2H7O X-ray 2.00 A 434-732 [» ]
    2H7V X-ray 2.60 C/D 434-732 [» ]
    ProteinModelPortali Q05608.
    SMRi Q05608. Positions 439-732.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q05608. 1 interaction.
    MINTi MINT-7717103.
    STRINGi 273123.pYV0001.

    PTM databases

    PhosSitei P010483.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAF25344 ; CAF25344 ; pYV0001 .
    GeneIDi 2952894.
    KEGGi yps:pYV0001.
    PATRICi 18637748. VBIYerPse22266_0001.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000219636.
    OMAi MRNFTAD.
    OrthoDBi EOG6NKQVS.

    Enzyme and pathway databases

    BioCyci YPSE273123:GI1M-4097-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q05608.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR019093. Rac1-binding_domain.
    IPR008271. Ser/Thr_kinase_AS.
    IPR003547. Ser/thr_kinase_yersinia-type.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF09632. Rac1. 1 hit.
    [Graphical view ]
    PRINTSi PR01373. YERSSTKINASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant."
      Galyov E.E., Haakansson S., Forsberg A., Wolf-Watz H.
      Nature 361:730-732(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YPIII / Serotype O:3.
      Plasmid: pIB1
    2. "Characterization of the operon encoding the YpkA Ser/Thr protein kinase and the YopJ protein of Yersinia pseudotuberculosis."
      Galyov E.E., Haakansson S., Wolf-Watz H.
      J. Bacteriol. 176:4543-4548(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YPIII / Serotype O:3.
      Plasmid: pIB1
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IP32953.
      Plasmid: pYV
    4. "The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane."
      Hakansson S., Galyov E.E., Rosqvist R., Wolf-Watz H.
      Mol. Microbiol. 20:593-603(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "A distinctive role for the Yersinia protein kinase: actin binding, kinase activation, and cytoskeleton disruption."
      Juris S.J., Rudolph A.E., Huddler D., Orth K., Dixon J.E.
      Proc. Natl. Acad. Sci. U.S.A. 97:9431-9436(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Identification of a molecular target for the Yersinia protein kinase A."
      Navarro L., Koller A., Nordfelth R., Wolf-Watz H., Taylor S., Dixon J.E.
      Mol. Cell 26:465-477(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors."
      Prehna G., Ivanov M.I., Bliska J.B., Stebbins C.E.
      Cell 126:869-880(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 434-732.

    Entry informationi

    Entry nameiYPKA_YERPS
    AccessioniPrimary (citable) accession number: Q05608
    Secondary accession number(s): Q663G6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3