ID   COBU_SALTY              Reviewed;         181 AA.
AC   Q05599;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   16-JUN-2009, entry version 72.
DE   RecName: Full=Bifunctional adenosylcobalamin biosynthesis protein cobU;
DE   Includes:
DE     RecName: Full=Adenosylcobinamide kinase;
DE              EC=2.7.1.156;
DE   Includes:
DE     RecName: Full=Adenosylcobinamide-phosphate guanylyltransferase;
DE              EC=2.7.7.62;
GN   Name=cobU; OrderedLocusNames=STM2018;
OS   Salmonella typhimurium.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90371;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   MEDLINE=93273696; PubMed=8501034;
RA   Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S.,
RA   Church G.M.;
RT   "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT   Salmonella typhimurium.";
RL   J. Bacteriol. 175:3303-3316(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   MEDLINE=21534948; PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
RA   Waterston R., Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium
RT   LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RC   STRAIN=LT2;
RX   MEDLINE=98263165; PubMed=9601028; DOI=10.1021/bi973178f;
RA   Thompson T.B., Thomas M.G., Escalante-Semerena J.C., Rayment I.;
RT   "Three-dimensional structure of adenosylcobinamide
RT   kinase/adenosylcobinamide phosphate guanylyltransferase from
RT   Salmonella typhimurium determined to 2.3-A resolution.";
RL   Biochemistry 37:7686-7695(1998).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX   MEDLINE=99459244; PubMed=10529169; DOI=10.1021/bi990910x;
RA   Thompson T.B., Thomas M.G., Escalante-Semerena J.C., Rayment I.;
RT   "Three-dimensional structure of adenosylcobinamide
RT   kinase/adenosylcobinamide phosphate guanylyltransferase (CobU)
RT   complexed with GMP: evidence for a substrate-induced transferase
RT   active site.";
RL   Biochemistry 38:12995-13005(1999).
CC   -!- FUNCTION: Catalyzes ATP-dependent phosphorylation of
CC       adenosylcobinamide and addition of GMP to adenosylcobinamide
CC       phosphate.
CC   -!- CATALYTIC ACTIVITY: ATP or GTP + adenosylcobinamide =
CC       adenosylcobinamide phosphate + ADP or GDP.
CC   -!- CATALYTIC ACTIVITY: GTP + adenosylcobinamide phosphate =
CC       diphosphate + adenosylcobinamide-GDP.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       adenosylcobalamin from cob(II)yrinic acid a,c-diamide: step 5/7.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       adenosylcobalamin from cob(II)yrinic acid a,c-diamide: step 6/7.
CC   -!- SUBUNIT: Homotrimer.
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DR   EMBL; L12006; AAA27269.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE008789; AAL20922.1; -; Genomic_DNA.
DR   RefSeq; NP_460963.1; -.
DR   PDB; 1C9K; X-ray; 2.20 A; A/B/C=2-181.
DR   PDB; 1CBU; X-ray; 2.30 A; A/B/C=2-181.
DR   PDBsum; 1C9K; -.
DR   PDBsum; 1CBU; -.
DR   GeneID; 1253539; -.
DR   GenomeReviews; AE006468_GR; STM2018.
DR   KEGG; stm:STM2018; -.
DR   HOGENOM; Q05599; -.
DR   OMA; Q05599; IDCLTLW.
DR   BioCyc; MetaCyc:MON-13215; -.
DR   BioCyc; STYP99287:STM2018-MON; -.
DR   BRENDA; 2.7.1.156; 2.
DR   BRENDA; 2.7.7.62; 2.
DR   GO; GO:0043752; F:adenosylcobinamide kinase activity; IEA:EC.
DR   GO; GO:0043753; F:adenosylcobinamide-phosphate guanylyltransf...; IEA:EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003203; Cobinamide_kinase/P_G-Trfase.
DR   Pfam; PF02283; CobU; 1.
DR   PIRSF; PIRSF006135; CobU; 1.
DR   ProDom; PD011091; CobU; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Cobalamin biosynthesis; Complete proteome;
KW   GTP-binding; Kinase; Multifunctional enzyme; Nucleotide-binding;
KW   Transferase.
FT   CHAIN         1    181       Bifunctional adenosylcobalamin
FT                                biosynthesis protein cobU.
FT                                /FTId=PRO_0000089998.
FT   NP_BIND       7     14       ATP (Potential).
FT   ACT_SITE     47     47       GMP-histidine intermediate.
FT   STRAND        3      7
FT   HELIX        13     21
FT   STRAND       25     31
FT   HELIX        43     49
FT   STRAND       55     58
FT   HELIX        64     66
FT   STRAND       77     81
FT   HELIX        83     93
FT   HELIX       105    125
FT   STRAND      128    133
FT   HELIX       146    165
FT   STRAND      167    173
FT   STRAND      176    179
SQ   SEQUENCE   181 AA;  19902 MW;  F6F89892C6BEE091 CRC64;
     MMILVTGGAR SGKSRHAEAL IGDAPQVLYI ATSQILDDEM AARIQHHKDG RPAHWRTAEC
     WRHLDTLITA DLAPDDAILL ECITTMVTNL LFALGGENDP EQWDYAAMER AIDDEIQILI
     AACQRCPAKV VLVTNEVGMG IVPENRLARH FRDIAGRVNQ RLAAAADEVW LVVSGIGVKI
     K
//