Q05599 (COBU_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional adenosylcobalamin biosynthesis protein CobU

Alternative name(s):
Adenosylcobinamide kinase
EC=2.7.1.156
Adenosylcobinamide-phosphate guanylyltransferase
EC=2.7.7.62

Gene names

Name:	cobU
Ordered Locus Names:	STM2018

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.
Catalytic activity	ATP or GTP + adenosylcobinamide = adenosylcobinamide phosphate + ADP or GDP. GTP + adenosylcobinamide phosphate = diphosphate + adenosylcobinamide-GDP.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 5/7. Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7.
Subunit structure	Homotrimer. Ref.3
Sequence caution	The sequence AAA27269.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosylcobinamide kinase activity Inferred from electronic annotation. Source: EC cobinamide phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 181

181

Bifunctional adenosylcobalamin biosynthesis protein CobU

PRO_0000089998

Regions

Nucleotide binding

7 – 14

8

GTP

Nucleotide binding

31 – 33

3

GTP

Nucleotide binding

48 – 51

4

GTP

Sites

Active site

47

1

GMP-histidine intermediate Ref.4

Binding site

59

1

GTP

Binding site

81

1

GTP; via carbonyl oxygen

Secondary structure

1

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181

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Helix

13 – 21

9

Beta strand

25 – 31

7

Helix

43 – 49

7

Beta strand

55 – 58

4

Helix

64 – 66

3

Beta strand

77 – 81

5

Helix

83 – 93

11

Helix

105 – 125

21

Beta strand

128 – 133

6

Helix

146 – 165

20

Beta strand

167 – 173

7

Beta strand

176 – 179

4

Sequences

	Sequence		Length	Mass (Da)
	Q05599 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: F6F89892C6BEE091	FASTA	181	19,902
	10 20 30 40 50 60 MMILVTGGAR SGKSRHAEAL IGDAPQVLYI ATSQILDDEM AARIQHHKDG RPAHWRTAEC 70 80 90 100 110 120 WRHLDTLITA DLAPDDAILL ECITTMVTNL LFALGGENDP EQWDYAAMER AIDDEIQILI 130 140 150 160 170 180 AACQRCPAKV VLVTNEVGMG IVPENRLARH FRDIAGRVNQ RLAAAADEVW LVVSGIGVKI K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium." Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M. J. Bacteriol. 175:3303-3316(1993) [PubMed: 8501034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3-A resolution." Thompson T.B., Thomas M.G., Escalante-Semerena J.C., Rayment I. Biochemistry 37:7686-7695(1998) [PubMed: 9601028] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SUBUNIT. Strain: LT2.
[4]	"Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site." Thompson T.B., Thomas M.G., Escalante-Semerena J.C., Rayment I. Biochemistry 38:12995-13005(1999) [PubMed: 10529169] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH GMP AND PHOSPHATE, ACTIVE SITE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L12006 Genomic DNA. Translation: AAA27269.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL20922.1.

RefSeq

NP_460963.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C9K	X-ray	2.20	A/B/C	2-181	[»]
1CBU	X-ray	2.30	A/B/C	2-181	[»]

ProteinModelPortal

Q05599.

SMR

Q05599. Positions 2-181.

ModBase

Search...

Proteomic databases

PRIDE

Q05599.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1253539.

GenomeReviews

Gene locus STM2018 in contig AE006468_GR.

KEGG

stm:STM2018.

PATRIC

32382619. VBISalEnt20916_2140.

Phylogenomic databases

HOGENOM

HBG635321.

OMA

TLWLTNH.

ProtClustDB

PRK05800.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-13215.
STYP99287:STM2018-MONOMER.

Family and domain databases

InterPro

IPR003203. Cobinamide_kinase/P_G-Trfase.
[Graphical view]

KO

K02231.

Pfam

PF02283. CobU. 1 hit.
[Graphical view]

PIRSF

PIRSF006135. CobU. 1 hit.

ProtoNet

Search...

Entry information

Entry name

COBU_SALTY

Accession

Primary (citable) accession number: Q05599

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program