Bifunctional adenosylcobalamin biosynthesis protein cobU

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Reviewed, UniProtKB/Swiss-Prot Q05599 (COBU_SALTY)

Last modified June 16, 2009. Version 72. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Bifunctional adenosylcobalamin biosynthesis protein cobU
Including the following 2 domains:
    1- Recommended name:
            Adenosylcobinamide kinase
              EC=2.7.1.156
    2- Recommended name:
            Adenosylcobinamide-phosphate guanylyltransferase
              EC=2.7.7.62

Gene names

Name:	cobU
Ordered Locus Names:	STM2018

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

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Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.
Catalytic activity	ATP or GTP + adenosylcobinamide = adenosylcobinamide phosphate + ADP or GDP. GTP + adenosylcobinamide phosphate = diphosphate + adenosylcobinamide-GDP.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinic acid a,c-diamide: step 5/7. Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinic acid a,c-diamide: step 6/7.
Subunit structure	Homotrimer.

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Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosylcobinamide kinase activity Inferred from electronic annotation. Source: EC adenosylcobinamide-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 181

181

Bifunctional adenosylcobalamin biosynthesis protein cobU

PRO_0000089998

Regions

Nucleotide binding

7 – 14

ATP Potential

Sites

Active site

GMP-histidine intermediate

Secondary structure

181

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q05599-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: F6F89892C6BEE091
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FASTA

181

19,902

        10         20         30         40         50         60 
MMILVTGGAR SGKSRHAEAL IGDAPQVLYI ATSQILDDEM AARIQHHKDG RPAHWRTAEC 

        70         80         90        100        110        120 
WRHLDTLITA DLAPDDAILL ECITTMVTNL LFALGGENDP EQWDYAAMER AIDDEIQILI 

       130        140        150        160        170        180 
AACQRCPAKV VLVTNEVGMG IVPENRLARH FRDIAGRVNQ RLAAAADEVW LVVSGIGVKI 


K

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium." Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M. J. Bacteriol. 175:3303-3316(1993) [PubMed: 8501034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3-A resolution." Thompson T.B., Thomas M.G., Escalante-Semerena J.C., Rayment I. Biochemistry 37:7686-7695(1998) [PubMed: 9601028] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS). Strain: LT2.
[4]	"Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site." Thompson T.B., Thomas M.G., Escalante-Semerena J.C., Rayment I. Biochemistry 38:12995-13005(1999) [PubMed: 10529169] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+	Additional computationally mapped references.

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Cross-references

Sequence databases

L12006 Genomic DNA. Translation: AAA27269.1. Different initiation.
AE008789 Genomic DNA. Translation: AAL20922.1.

RefSeq

NP_460963.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C9K	X-ray	2.20	A/B/C	2-181	[»]
1CBU	X-ray	2.30	A/B/C	2-181	[»]

ModBase

Search...

Genome annotation databases

GeneID

1253539.

GenomeReviews

Gene locus STM2018 in contig AE006468_GR.

KEGG

stm:STM2018.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q05599.

OMA

Q05599. IDCLTLW.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-13215.
STYP99287:STM2018-MON.

BRENDA

2.7.1.156. 2.
2.7.7.62. 2.

Family and domain databases

InterPro

IPR003203. Cobinamide_kinase/P_G-Trfase.
[Graphical view]

Pfam

PF02283. CobU. 1 hit.
[Graphical view]

PIRSF

PIRSF006135. CobU. 1 hit.

ProDom

PD011091. CobU. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

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Entry information

Entry name

COBU_SALTY

Accession

Primary (citable) accession number: Q05599

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references