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Protein

Cobyric acid synthase

Gene

cbiP

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.

Pathway: adenosylcobalamin biosynthesis

This protein is involved in the pathway adenosylcobalamin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei332 – 3321NucleophileBy similarity
Active sitei440 – 4401By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cobalamin biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13223.
SENT99287:GCTI-2031-MONOMER.
UniPathwayiUPA00148.

Names & Taxonomyi

Protein namesi
Recommended name:
Cobyric acid synthase
Gene namesi
Name:cbiP
Ordered Locus Names:STM2019
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Cobyric acid synthasePRO_0000141329Add
BLAST

Proteomic databases

PRIDEiQ05597.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi99287.STM2019.

Structurei

3D structure databases

ProteinModelPortaliQ05597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini251 – 448198GATase cobBQ-typeAdd
BLAST

Sequence similaritiesi

Belongs to the CobB/CobQ family. CobQ subfamily.Curated
Contains 1 GATase cobBQ-type domain.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000224803.
KOiK02232.
OMAiWLDIDLE.
OrthoDBiEOG6M9F07.
PhylomeDBiQ05597.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00028. CobQ.
InterProiIPR029062. Class_I_gatase-like.
IPR017929. CobB/CobQ_GATase.
IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom.
IPR004459. CobQ_synth.
IPR011698. GATase_3.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01656. CbiA. 1 hit.
PF07685. GATase_3. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00313. cobQ. 1 hit.
PROSITEiPS51274. GATASE_COBBQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQAVMLQGT ASDVGKSVLA AGLCRIFYQD GLRTAPFKSQ NMALNSGITP
60 70 80 90 100
DGKEMGRAQI FQAEAAGITP DVRMNPVLLK PTSDRQAQVV LMGKVATNMD
110 120 130 140 150
AVSYHDYKPR LREQILAVYN SLAQEYDVIV LEGAGSPAEI NLRDRDIVNM
160 170 180 190 200
GMAEMAQCPV ILVADIDRGG VFAAIYGTLA LLHKQERDRV KGVIINKFRG
210 220 230 240 250
DVALLYSGIE QIESLTGVPV LGVMPWLDVD LEDEDGVALQ NDKYRGNAPR
260 270 280 290 300
DITIAIVQLP HISNFTDFNA LAAQPDVRIR YIRRPEALTD VDLVILPGSK
310 320 330 340 350
NTLSDLAWLR ESGMADAVLQ THRQGVPVMG ICGGYQMLGD TIVDEVESGL
360 370 380 390 400
GTQPGLGLLN TITRFAQDKT TTQVNATMSG ELPGWLAAAA GLPVRGYEIH
410 420 430 440 450
MGETVLQEGC CTAMTLQKNG CSVADGAVTA DGLAFGTYLH GLFDSDAFTR
460 470 480 490 500
AVVNGLRARK GLAPWETTFC YADHKARQFD LLAEAMRQHI DIDKIYTIMQ

QHQEPV
Length:506
Mass (Da):54,967
Last modified:June 1, 1994 - v1
Checksum:i4F7658E1ADB36CE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12006 Genomic DNA. Translation: AAA27268.1.
AE006468 Genomic DNA. Translation: AAL20923.1.
RefSeqiNP_460964.1. NC_003197.1.
WP_000189662.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20923; AAL20923; STM2019.
GeneIDi1253540.
KEGGistm:STM2019.
PATRICi32382621. VBISalEnt20916_2141.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12006 Genomic DNA. Translation: AAA27268.1.
AE006468 Genomic DNA. Translation: AAL20923.1.
RefSeqiNP_460964.1. NC_003197.1.
WP_000189662.1. NC_003197.1.

3D structure databases

ProteinModelPortaliQ05597.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2019.

Proteomic databases

PRIDEiQ05597.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20923; AAL20923; STM2019.
GeneIDi1253540.
KEGGistm:STM2019.
PATRICi32382621. VBISalEnt20916_2141.

Phylogenomic databases

HOGENOMiHOG000224803.
KOiK02232.
OMAiWLDIDLE.
OrthoDBiEOG6M9F07.
PhylomeDBiQ05597.

Enzyme and pathway databases

UniPathwayiUPA00148.
BioCyciMetaCyc:MONOMER-13223.
SENT99287:GCTI-2031-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00028. CobQ.
InterProiIPR029062. Class_I_gatase-like.
IPR017929. CobB/CobQ_GATase.
IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom.
IPR004459. CobQ_synth.
IPR011698. GATase_3.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01656. CbiA. 1 hit.
PF07685. GATase_3. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00313. cobQ. 1 hit.
PROSITEiPS51274. GATASE_COBBQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium."
    Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.
    J. Bacteriol. 175:3303-3316(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiCBIP_SALTY
AccessioniPrimary (citable) accession number: Q05597
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 27, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.