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Q05597 (CBIP_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cobyric acid synthase
Gene names
Name:cbiP
Ordered Locus Names:STM2019
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation. HAMAP-Rule MF_00028

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00028

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00028

Sequence similarities

Belongs to the CobB/CobQ family. CobQ subfamily.

Contains 1 GATase cobBQ-type domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Cobyric acid synthase HAMAP-Rule MF_00028
PRO_0000141329

Regions

Domain251 – 448198GATase cobBQ-type

Sites

Active site3321Nucleophile By similarity
Active site4401 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05597 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 4F7658E1ADB36CE7

FASTA50654,967
        10         20         30         40         50         60 
MTQAVMLQGT ASDVGKSVLA AGLCRIFYQD GLRTAPFKSQ NMALNSGITP DGKEMGRAQI 

        70         80         90        100        110        120 
FQAEAAGITP DVRMNPVLLK PTSDRQAQVV LMGKVATNMD AVSYHDYKPR LREQILAVYN 

       130        140        150        160        170        180 
SLAQEYDVIV LEGAGSPAEI NLRDRDIVNM GMAEMAQCPV ILVADIDRGG VFAAIYGTLA 

       190        200        210        220        230        240 
LLHKQERDRV KGVIINKFRG DVALLYSGIE QIESLTGVPV LGVMPWLDVD LEDEDGVALQ 

       250        260        270        280        290        300 
NDKYRGNAPR DITIAIVQLP HISNFTDFNA LAAQPDVRIR YIRRPEALTD VDLVILPGSK 

       310        320        330        340        350        360 
NTLSDLAWLR ESGMADAVLQ THRQGVPVMG ICGGYQMLGD TIVDEVESGL GTQPGLGLLN 

       370        380        390        400        410        420 
TITRFAQDKT TTQVNATMSG ELPGWLAAAA GLPVRGYEIH MGETVLQEGC CTAMTLQKNG 

       430        440        450        460        470        480 
CSVADGAVTA DGLAFGTYLH GLFDSDAFTR AVVNGLRARK GLAPWETTFC YADHKARQFD 

       490        500 
LLAEAMRQHI DIDKIYTIMQ QHQEPV 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium."
Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.
J. Bacteriol. 175:3303-3316(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT2.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12006 Genomic DNA. Translation: AAA27268.1.
AE006468 Genomic DNA. Translation: AAL20923.1.
RefSeqNP_460964.1. NC_003197.1.

3D structure databases

ProteinModelPortalQ05597.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM2019.

Proteomic databases

PRIDEQ05597.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL20923; AAL20923; STM2019.
GeneID1253540.
KEGGstm:STM2019.
PATRIC32382621. VBISalEnt20916_2141.

Phylogenomic databases

HOGENOMHOG000224803.
KOK02232.
OMAAHTDLNP.
OrthoDBEOG6M9F07.
ProtClustDBPRK00784.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13223.
SENT99287:GCTI-2031-MONOMER.
UniPathwayUPA00148.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_00028. CobQ.
InterProIPR017929. CobB/CobQ_GATase.
IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom.
IPR004459. CobQ_synth.
IPR011698. GATase_3.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01656. CbiA. 1 hit.
PF07685. GATase_3. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00313. cobQ. 1 hit.
PROSITEPS51274. GATASE_COBBQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCBIP_SALTY
AccessionPrimary (citable) accession number: Q05597
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways