Q05597 (CBIP_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cobyric acid synthase

Gene names

Name:	cbiP
Ordered Locus Names:	STM2019

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation. HAMAP-Rule MF_00028
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00028
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the CobB/CobQ family. CobQ subfamily. Contains 1 GATase cobBQ-type domain.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Domain	Glutamine amidotransferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Cobyric acid synthase HAMAP-Rule MF_00028		PRO_0000141329
Regions
	Domain	251 – 448	198	GATase cobBQ-type
Sites
	Active site	332	1	Nucleophile By similarity
	Active site	440	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05597 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 4F7658E1ADB36CE7	FASTA	506	54,967
	10 20 30 40 50 60 MTQAVMLQGT ASDVGKSVLA AGLCRIFYQD GLRTAPFKSQ NMALNSGITP DGKEMGRAQI 70 80 90 100 110 120 FQAEAAGITP DVRMNPVLLK PTSDRQAQVV LMGKVATNMD AVSYHDYKPR LREQILAVYN 130 140 150 160 170 180 SLAQEYDVIV LEGAGSPAEI NLRDRDIVNM GMAEMAQCPV ILVADIDRGG VFAAIYGTLA 190 200 210 220 230 240 LLHKQERDRV KGVIINKFRG DVALLYSGIE QIESLTGVPV LGVMPWLDVD LEDEDGVALQ 250 260 270 280 290 300 NDKYRGNAPR DITIAIVQLP HISNFTDFNA LAAQPDVRIR YIRRPEALTD VDLVILPGSK 310 320 330 340 350 360 NTLSDLAWLR ESGMADAVLQ THRQGVPVMG ICGGYQMLGD TIVDEVESGL GTQPGLGLLN 370 380 390 400 410 420 TITRFAQDKT TTQVNATMSG ELPGWLAAAA GLPVRGYEIH MGETVLQEGC CTAMTLQKNG 430 440 450 460 470 480 CSVADGAVTA DGLAFGTYLH GLFDSDAFTR AVVNGLRARK GLAPWETTFC YADHKARQFD 490 500 LLAEAMRQHI DIDKIYTIMQ QHQEPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium." Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M. J. Bacteriol. 175:3303-3316(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12006 Genomic DNA. Translation: AAA27268.1. AE006468 Genomic DNA. Translation: AAL20923.1.
RefSeq	NP_460964.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q05597.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM2019.
Proteomic databases
PRIDE	Q05597.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL20923; AAL20923; STM2019.
GeneID	1253540.
KEGG	stm:STM2019.
PATRIC	32382621. VBISalEnt20916_2141.
Phylogenomic databases
HOGENOM	HOG000224803.
KO	K02232.
OMA	HMGETIL.
ProtClustDB	PRK00784.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13223.
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00028. CobQ.
InterPro	IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR004459. CobQ_synth. IPR011698. GATase_3. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00313. cobQ. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBIP_SALTY

Accession

Primary (citable) accession number: Q05597

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program