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Q05589 (UROK_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Urokinase-type plasminogen activator

Short name=U-plasminogen activator
Short name=uPA
EC=3.4.21.73
Gene names
Name:PLAU
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length433 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activity

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulation

Inhibited by SERPINA5 By similarity.

Subunit structure

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.

Subcellular location

Secreted.

Induction

By retinoic acid.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 EGF-like domain.

Contains 1 kringle domain.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 433413Urokinase-type plasminogen activator
PRO_0000028315
Chain21 – 179159Urokinase-type plasminogen activator long chain A By similarity
PRO_0000028316
Chain158 – 17922Urokinase-type plasminogen activator short chain A By similarity
PRO_0000285895
Chain181 – 433253Urokinase-type plasminogen activator chain B By similarity
PRO_0000028317

Regions

Domain29 – 6537EGF-like
Domain72 – 15382Kringle
Domain181 – 426246Peptidase S1
Region36 – 5924Binds urokinase plasminogen activator surface receptor By similarity
Region154 – 18027Connecting peptide

Sites

Active site2261Charge relay system By similarity
Active site2771Charge relay system By similarity
Active site3781Charge relay system By similarity

Amino acid modifications

Modified residue1601Phosphoserine By similarity
Disulfide bond33 ↔ 41 By similarity
Disulfide bond35 ↔ 53 By similarity
Disulfide bond55 ↔ 64 By similarity
Disulfide bond72 ↔ 153 By similarity
Disulfide bond93 ↔ 135 By similarity
Disulfide bond124 ↔ 148 By similarity
Disulfide bond170 ↔ 301Interchain (between A and B chains) By similarity
Disulfide bond211 ↔ 227 By similarity
Disulfide bond219 ↔ 290 By similarity
Disulfide bond315 ↔ 384 By similarity
Disulfide bond347 ↔ 363 By similarity
Disulfide bond374 ↔ 402 By similarity

Experimental info

Sequence conflict1891A → T in CAA59796. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q05589 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 4DE1B8D4DA47027A

FASTA43348,730
        10         20         30         40         50         60 
MRVLLACLLV CALVVSDSDG SNEVHKESGE SNCGCLNGGK CVTYKYFSNI QRCSCPKKFQ 

        70         80         90        100        110        120 
GEHCEIDTSK TCYQGNGHSY RGKANRDLSG RPCLAWDSPT VLLKMYHAHR SDAIQLGLGK 

       130        140        150        160        170        180 
HNYCRNPDNQ RRPWCYVQIG LKQFVQFCMV QDCSVGKSPS SPREKEEFQC GQKALRPRFK 

       190        200        210        220        230        240 
IVGGQVTNAE NQPWFAAIYR RHRGGSITYL CGGSLISPCW VVSATHCFID HPKKENYIVY 

       250        260        270        280        290        300 
LGQSRLNSDT RGEMQFEVEK LILHEDYSAE SLAHHNDIAL LKIRTSRGQC AQPSRSIQTI 

       310        320        330        340        350        360 
CLPPEHEDAH SRTRCEITGF GKENPSDYRY SDELKMTFVS LVSHEVCQQP HYYGAEVTDK 

       370        380        390        400        410        420 
MLCAADPQWE TDSCQGDSGG PLVCTIQGRL TLTGIVSWGR DCAMKYKPGV YTRVSKFLPW 

       430 
INTHTRGEIN LVL 

« Hide

References

« Hide 'large scale' references
[1]"Bovine urokinase-type plasminogen activator and its receptor: cloning and induction by retinoic acid."
Kraetzschmar J., Haendler B., Kojima S., Rifkin D.B., Schleuning W.-D.
Gene 125:177-183(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Aortic endothelium.
[2]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal skin.
[4]"Cloning and characterization of the bovine plasminogen activators uPA and tPA."
Ravn P., Berglund L., Petersen T.E.
Int. Dairy J. 5:605-617(1995) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-433.
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L03546 mRNA. Translation: AAA51419.1.
BT030737 mRNA. Translation: ABS45053.1.
BC122657 mRNA. Translation: AAI22658.1.
X85801 mRNA. Translation: CAA59796.1.
PIRJN0560.
RefSeqNP_776572.1. NM_174147.2.
XP_005226381.1. XM_005226324.1.
UniGeneBt.2519.

3D structure databases

ProteinModelPortalQ05589.
SMRQ05589. Positions 31-157, 168-428.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.231.

Proteomic databases

PRIDEQ05589.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000007806; ENSBTAP00000007806; ENSBTAG00000005947.
GeneID281408.
KEGGbta:281408.

Organism-specific databases

CTD5328.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00740000115235.
HOGENOMHOG000237314.
HOVERGENHBG008633.
InParanoidQ05589.
KOK01348.
OMAKMLCAAD.
OrthoDBEOG75B84T.
TreeFamTF329901.

Family and domain databases

Gene3D2.40.20.10. 1 hit.
InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805398.

Entry information

Entry nameUROK_BOVIN
AccessionPrimary (citable) accession number: Q05589
Secondary accession number(s): A7E3W6, Q0IIG3, Q28209
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries