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Q05589

- UROK_BOVIN

UniProt

Q05589 - UROK_BOVIN

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Protein
Urokinase-type plasminogen activator
Gene
PLAU
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei226 – 2261Charge relay system By similarity
Active sitei277 – 2771Charge relay system By similarity
Active sitei378 – 3781Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. fibrinolysis Source: Ensembl
  2. regulation of cell adhesion mediated by integrin Source: Ensembl
  3. regulation of cell proliferation Source: Ensembl
  4. regulation of receptor activity Source: Ensembl
  5. regulation of smooth muscle cell migration Source: Ensembl
  6. regulation of smooth muscle cell-matrix adhesion Source: Ensembl
  7. response to hypoxia Source: Ensembl
  8. smooth muscle cell migration Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Plasminogen activation

Enzyme and pathway databases

ReactomeiREACT_207584. Dissolution of Fibrin Clot.

Protein family/group databases

MEROPSiS01.231.

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:PLAU
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 28

Subcellular locationi

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. extracellular space Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020
Add
BLAST
Chaini21 – 433413Urokinase-type plasminogen activator
PRO_0000028315Add
BLAST
Chaini21 – 179159Urokinase-type plasminogen activator long chain A By similarity
PRO_0000028316Add
BLAST
Chaini158 – 17922Urokinase-type plasminogen activator short chain A By similarity
PRO_0000285895Add
BLAST
Chaini181 – 433253Urokinase-type plasminogen activator chain B By similarity
PRO_0000028317Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 41 By similarity
Disulfide bondi35 ↔ 53 By similarity
Disulfide bondi55 ↔ 64 By similarity
Disulfide bondi72 ↔ 153 By similarity
Disulfide bondi93 ↔ 135 By similarity
Disulfide bondi124 ↔ 148 By similarity
Modified residuei160 – 1601Phosphoserine By similarity
Disulfide bondi170 ↔ 301Interchain (between A and B chains) By similarity
Disulfide bondi211 ↔ 227 By similarity
Disulfide bondi219 ↔ 290 By similarity
Disulfide bondi315 ↔ 384 By similarity
Disulfide bondi347 ↔ 363 By similarity
Disulfide bondi374 ↔ 402 By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PRIDEiQ05589.

Expressioni

Inductioni

By retinoic acid.

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ05589.
SMRiQ05589. Positions 31-157, 168-428.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 6537EGF-like
Add
BLAST
Domaini72 – 15382Kringle
Add
BLAST
Domaini181 – 426246Peptidase S1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 5924Binds urokinase plasminogen activator surface receptor By similarity
Add
BLAST
Regioni154 – 18027Connecting peptide
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00740000115235.
HOGENOMiHOG000237314.
HOVERGENiHBG008633.
InParanoidiQ05589.
KOiK01348.
OMAiKMLCAAD.
OrthoDBiEOG75B84T.
TreeFamiTF329901.

Family and domain databases

Gene3Di2.40.20.10. 1 hit.
InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05589-1 [UniParc]FASTAAdd to Basket

« Hide

MRVLLACLLV CALVVSDSDG SNEVHKESGE SNCGCLNGGK CVTYKYFSNI    50
QRCSCPKKFQ GEHCEIDTSK TCYQGNGHSY RGKANRDLSG RPCLAWDSPT 100
VLLKMYHAHR SDAIQLGLGK HNYCRNPDNQ RRPWCYVQIG LKQFVQFCMV 150
QDCSVGKSPS SPREKEEFQC GQKALRPRFK IVGGQVTNAE NQPWFAAIYR 200
RHRGGSITYL CGGSLISPCW VVSATHCFID HPKKENYIVY LGQSRLNSDT 250
RGEMQFEVEK LILHEDYSAE SLAHHNDIAL LKIRTSRGQC AQPSRSIQTI 300
CLPPEHEDAH SRTRCEITGF GKENPSDYRY SDELKMTFVS LVSHEVCQQP 350
HYYGAEVTDK MLCAADPQWE TDSCQGDSGG PLVCTIQGRL TLTGIVSWGR 400
DCAMKYKPGV YTRVSKFLPW INTHTRGEIN LVL 433
Length:433
Mass (Da):48,730
Last modified:February 1, 1994 - v1
Checksum:i4DE1B8D4DA47027A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti189 – 1891A → T in CAA59796. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L03546 mRNA. Translation: AAA51419.1.
BT030737 mRNA. Translation: ABS45053.1.
BC122657 mRNA. Translation: AAI22658.1.
X85801 mRNA. Translation: CAA59796.1.
PIRiJN0560.
RefSeqiNP_776572.1. NM_174147.2.
XP_005226381.1. XM_005226324.1.
UniGeneiBt.2519.

Genome annotation databases

EnsembliENSBTAT00000007806; ENSBTAP00000007806; ENSBTAG00000005947.
GeneIDi281408.
KEGGibta:281408.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L03546 mRNA. Translation: AAA51419.1 .
BT030737 mRNA. Translation: ABS45053.1 .
BC122657 mRNA. Translation: AAI22658.1 .
X85801 mRNA. Translation: CAA59796.1 .
PIRi JN0560.
RefSeqi NP_776572.1. NM_174147.2.
XP_005226381.1. XM_005226324.1.
UniGenei Bt.2519.

3D structure databases

ProteinModelPortali Q05589.
SMRi Q05589. Positions 31-157, 168-428.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.231.

Proteomic databases

PRIDEi Q05589.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000007806 ; ENSBTAP00000007806 ; ENSBTAG00000005947 .
GeneIDi 281408.
KEGGi bta:281408.

Organism-specific databases

CTDi 5328.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00740000115235.
HOGENOMi HOG000237314.
HOVERGENi HBG008633.
InParanoidi Q05589.
KOi K01348.
OMAi KMLCAAD.
OrthoDBi EOG75B84T.
TreeFami TF329901.

Enzyme and pathway databases

Reactomei REACT_207584. Dissolution of Fibrin Clot.

Miscellaneous databases

NextBioi 20805398.

Family and domain databases

Gene3Di 2.40.20.10. 1 hit.
InterProi IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEi PS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bovine urokinase-type plasminogen activator and its receptor: cloning and induction by retinoic acid."
    Kraetzschmar J., Haendler B., Kojima S., Rifkin D.B., Schleuning W.-D.
    Gene 125:177-183(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Aortic endothelium.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.
  4. "Cloning and characterization of the bovine plasminogen activators uPA and tPA."
    Ravn P., Berglund L., Petersen T.E.
    Int. Dairy J. 5:605-617(1995)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-433.
    Tissue: Kidney.

Entry informationi

Entry nameiUROK_BOVIN
AccessioniPrimary (citable) accession number: Q05589
Secondary accession number(s): A7E3W6, Q0IIG3, Q28209
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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