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Q05589

- UROK_BOVIN

UniProt

Q05589 - UROK_BOVIN

Protein

Urokinase-type plasminogen activator

Gene

PLAU

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

    Catalytic activityi

    Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

    Enzyme regulationi

    Inhibited by SERPINA5.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei226 – 2261Charge relay systemBy similarity
    Active sitei277 – 2771Charge relay systemBy similarity
    Active sitei378 – 3781Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. fibrinolysis Source: Ensembl
    2. regulation of cell adhesion mediated by integrin Source: Ensembl
    3. regulation of cell proliferation Source: Ensembl
    4. regulation of receptor activity Source: Ensembl
    5. regulation of smooth muscle cell-matrix adhesion Source: Ensembl
    6. regulation of smooth muscle cell migration Source: Ensembl
    7. response to hypoxia Source: Ensembl
    8. smooth muscle cell migration Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Plasminogen activation

    Enzyme and pathway databases

    ReactomeiREACT_207584. Dissolution of Fibrin Clot.

    Protein family/group databases

    MEROPSiS01.231.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Urokinase-type plasminogen activator (EC:3.4.21.73)
    Short name:
    U-plasminogen activator
    Short name:
    uPA
    Cleaved into the following 3 chains:
    Gene namesi
    Name:PLAU
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 28

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: Ensembl
    2. extracellular space Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Add
    BLAST
    Chaini21 – 433413Urokinase-type plasminogen activatorPRO_0000028315Add
    BLAST
    Chaini21 – 179159Urokinase-type plasminogen activator long chain ABy similarityPRO_0000028316Add
    BLAST
    Chaini158 – 17922Urokinase-type plasminogen activator short chain ABy similarityPRO_0000285895Add
    BLAST
    Chaini181 – 433253Urokinase-type plasminogen activator chain BBy similarityPRO_0000028317Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 41By similarity
    Disulfide bondi35 ↔ 53By similarity
    Disulfide bondi55 ↔ 64By similarity
    Disulfide bondi72 ↔ 153By similarity
    Disulfide bondi93 ↔ 135By similarity
    Disulfide bondi124 ↔ 148By similarity
    Modified residuei160 – 1601PhosphoserineBy similarity
    Disulfide bondi170 ↔ 301Interchain (between A and B chains)PROSITE-ProRule annotation
    Disulfide bondi211 ↔ 227By similarity
    Disulfide bondi219 ↔ 290By similarity
    Disulfide bondi315 ↔ 384By similarity
    Disulfide bondi347 ↔ 363By similarity
    Disulfide bondi374 ↔ 402By similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein, Zymogen

    Proteomic databases

    PRIDEiQ05589.

    Expressioni

    Inductioni

    By retinoic acid.

    Interactioni

    Subunit structurei

    Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ05589.
    SMRiQ05589. Positions 31-157, 168-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 6537EGF-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini72 – 15382KringlePROSITE-ProRule annotationAdd
    BLAST
    Domaini181 – 426246Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni36 – 5924Binds urokinase plasminogen activator surface receptorBy similarityAdd
    BLAST
    Regioni154 – 18027Connecting peptideAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 kringle domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Kringle, Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00740000115235.
    HOGENOMiHOG000237314.
    HOVERGENiHBG008633.
    InParanoidiQ05589.
    KOiK01348.
    OMAiKMLCAAD.
    OrthoDBiEOG75B84T.
    TreeFamiTF329901.

    Family and domain databases

    Gene3Di2.40.20.10. 1 hit.
    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 1 hit.
    PROSITEiPS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05589-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVLLACLLV CALVVSDSDG SNEVHKESGE SNCGCLNGGK CVTYKYFSNI    50
    QRCSCPKKFQ GEHCEIDTSK TCYQGNGHSY RGKANRDLSG RPCLAWDSPT 100
    VLLKMYHAHR SDAIQLGLGK HNYCRNPDNQ RRPWCYVQIG LKQFVQFCMV 150
    QDCSVGKSPS SPREKEEFQC GQKALRPRFK IVGGQVTNAE NQPWFAAIYR 200
    RHRGGSITYL CGGSLISPCW VVSATHCFID HPKKENYIVY LGQSRLNSDT 250
    RGEMQFEVEK LILHEDYSAE SLAHHNDIAL LKIRTSRGQC AQPSRSIQTI 300
    CLPPEHEDAH SRTRCEITGF GKENPSDYRY SDELKMTFVS LVSHEVCQQP 350
    HYYGAEVTDK MLCAADPQWE TDSCQGDSGG PLVCTIQGRL TLTGIVSWGR 400
    DCAMKYKPGV YTRVSKFLPW INTHTRGEIN LVL 433
    Length:433
    Mass (Da):48,730
    Last modified:February 1, 1994 - v1
    Checksum:i4DE1B8D4DA47027A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti189 – 1891A → T in CAA59796. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03546 mRNA. Translation: AAA51419.1.
    BT030737 mRNA. Translation: ABS45053.1.
    BC122657 mRNA. Translation: AAI22658.1.
    X85801 mRNA. Translation: CAA59796.1.
    PIRiJN0560.
    RefSeqiNP_776572.1. NM_174147.2.
    XP_005226381.1. XM_005226324.1.
    UniGeneiBt.2519.

    Genome annotation databases

    EnsembliENSBTAT00000007806; ENSBTAP00000007806; ENSBTAG00000005947.
    GeneIDi281408.
    KEGGibta:281408.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03546 mRNA. Translation: AAA51419.1 .
    BT030737 mRNA. Translation: ABS45053.1 .
    BC122657 mRNA. Translation: AAI22658.1 .
    X85801 mRNA. Translation: CAA59796.1 .
    PIRi JN0560.
    RefSeqi NP_776572.1. NM_174147.2.
    XP_005226381.1. XM_005226324.1.
    UniGenei Bt.2519.

    3D structure databases

    ProteinModelPortali Q05589.
    SMRi Q05589. Positions 31-157, 168-428.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.231.

    Proteomic databases

    PRIDEi Q05589.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000007806 ; ENSBTAP00000007806 ; ENSBTAG00000005947 .
    GeneIDi 281408.
    KEGGi bta:281408.

    Organism-specific databases

    CTDi 5328.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00740000115235.
    HOGENOMi HOG000237314.
    HOVERGENi HBG008633.
    InParanoidi Q05589.
    KOi K01348.
    OMAi KMLCAAD.
    OrthoDBi EOG75B84T.
    TreeFami TF329901.

    Enzyme and pathway databases

    Reactomei REACT_207584. Dissolution of Fibrin Clot.

    Miscellaneous databases

    NextBioi 20805398.

    Family and domain databases

    Gene3Di 2.40.20.10. 1 hit.
    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 1 hit.
    PROSITEi PS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bovine urokinase-type plasminogen activator and its receptor: cloning and induction by retinoic acid."
      Kraetzschmar J., Haendler B., Kojima S., Rifkin D.B., Schleuning W.-D.
      Gene 125:177-183(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Aortic endothelium.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal skin.
    4. "Cloning and characterization of the bovine plasminogen activators uPA and tPA."
      Ravn P., Berglund L., Petersen T.E.
      Int. Dairy J. 5:605-617(1995)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-433.
      Tissue: Kidney.

    Entry informationi

    Entry nameiUROK_BOVIN
    AccessioniPrimary (citable) accession number: Q05589
    Secondary accession number(s): A7E3W6, Q0IIG3, Q28209
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3