Reviewed, UniProtKB/Swiss-Prot Q05589 (UROK_BOVIN)

Names and origin

Protein names

Recommended name:
    Urokinase-type plasminogen activator
      Short name=U-plasminogen activator
      Short name=uPA
    EC=3.4.21.73
Cleaved into the following 3 chains:
    1- Recommended name:
            Urokinase-type plasminogen activator long chain A
    2- Recommended name:
            Urokinase-type plasminogen activator short chain A
    3- Recommended name:
            Urokinase-type plasminogen activator chain B

Gene names

Name:

PLAU

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
Catalytic activity	Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Subunit structure	Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.
Subcellular location	Secreted.
Induction	By retinoic acid.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Plasminogen activation
Cellular component	Secreted
Domain	EGF-like domain Kringle Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 433	413	Urokinase-type plasminogen activator		PRO_0000028315
	Chain	21 – 179	159	Urokinase-type plasminogen activator long chain A By similarity		PRO_0000028316
	Chain	158 – 179	22	Urokinase-type plasminogen activator short chain A By similarity		PRO_0000285895
	Chain	181 – 433	253	Urokinase-type plasminogen activator chain B By similarity		PRO_0000028317
Regions
	Domain	29 – 65	37	EGF-like
	Domain	72 – 153	82	Kringle
	Domain	181 – 426	246	Peptidase S1
	Region	36 – 59	24	Binds urokinase plasminogen activator surface receptor By similarity
	Region	154 – 180	27	Connecting peptide
Sites
	Active site	226	1	Charge relay system By similarity
	Active site	277	1	Charge relay system By similarity
	Active site	378	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	33 ↔ 41		By similarity
	Disulfide bond	35 ↔ 53		By similarity
	Disulfide bond	55 ↔ 64		By similarity
	Disulfide bond	72 ↔ 153		By similarity
	Disulfide bond	93 ↔ 135		By similarity
	Disulfide bond	124 ↔ 148		By similarity
	Disulfide bond	170 ↔ 301		Interchain (between A and B chains) By similarity
	Disulfide bond	211 ↔ 227		By similarity
	Disulfide bond	219 ↔ 290		By similarity
	Disulfide bond	315 ↔ 384		By similarity
	Disulfide bond	347 ↔ 363		By similarity
	Disulfide bond	374 ↔ 402		By similarity
Experimental info
	Sequence conflict	189	1	A → T in CAA59796. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q05589-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 4DE1B8D4DA47027A	FASTA	433	48,730
	10 20 30 40 50 60 MRVLLACLLV CALVVSDSDG SNEVHKESGE SNCGCLNGGK CVTYKYFSNI QRCSCPKKFQ 70 80 90 100 110 120 GEHCEIDTSK TCYQGNGHSY RGKANRDLSG RPCLAWDSPT VLLKMYHAHR SDAIQLGLGK 130 140 150 160 170 180 HNYCRNPDNQ RRPWCYVQIG LKQFVQFCMV QDCSVGKSPS SPREKEEFQC GQKALRPRFK 190 200 210 220 230 240 IVGGQVTNAE NQPWFAAIYR RHRGGSITYL CGGSLISPCW VVSATHCFID HPKKENYIVY 250 260 270 280 290 300 LGQSRLNSDT RGEMQFEVEK LILHEDYSAE SLAHHNDIAL LKIRTSRGQC AQPSRSIQTI 310 320 330 340 350 360 CLPPEHEDAH SRTRCEITGF GKENPSDYRY SDELKMTFVS LVSHEVCQQP HYYGAEVTDK 370 380 390 400 410 420 MLCAADPQWE TDSCQGDSGG PLVCTIQGRL TLTGIVSWGR DCAMKYKPGV YTRVSKFLPW 430 INTHTRGEIN LVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bovine urokinase-type plasminogen activator and its receptor: cloning and induction by retinoic acid." Kraetzschmar J., Haendler B., Kojima S., Rifkin D.B., Schleuning W.-D. Gene 125:177-183(1993) [PubMed: 8385052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Aortic endothelium.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal skin.
[4]	"Cloning and characterization of the bovine plasminogen activators uPA and tPA." Ravn P., Berglund L., Petersen T.E. Int. Dairy J. 5:605-617(1995) [Agricola: IND20546782] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-433. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L03546 mRNA. Translation: AAA51419.1. BT030737 mRNA. Translation: ABS45053.1. BC122657 mRNA. Translation: AAI22658.1. X85801 mRNA. Translation: CAA59796.1.
IPI	IPI00695226.
PIR	JN0560.
RefSeq	NP_776572.1.
UniGene	Bt.2519
3D structure databases
HSSP	HSSP built from PDB template 1LMW based on UniProtKB P00749.
SMR	Q05589. Positions 31-154, 181-426.
ModBase	Search...
Protein-protein interaction databases
STRING	Q05589.
Protein family/group databases
MEROPS	S01.231.
Genome annotation databases
Ensembl	ENSBTAT00000007806; ENSBTAP00000007806; ENSBTAG00000005947; Bos taurus. [Genome view]
GeneID	281408.
KEGG	bta:281408.
Organism-specific databases
CTD	281408.
Phylogenomic databases
HOVERGEN	Q05589.
OMA	QPWFAAI.
Enzyme and pathway databases
BRENDA	3.4.21.73. 251.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR000001. Kringle. IPR018056. Kringle_CS. IPR018059. Kringle_sub. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Gene3D	G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00018. KRINGLE.
ProDom	PD000395. Kringle. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. False negative. PS50026. EGF_3. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UROK_BOVIN

Accession

Primary (citable) accession number: Q05589
Secondary accession number(s): A7E3W6, Q0IIG3, Q28209

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)