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Protein

Hydroxyacylglutathione hydrolase, cytoplasmic isozyme

Gene

GLO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (GLO1)
  2. Hydroxyacylglutathione hydrolase, mitochondrial (GLO4), Hydroxyacylglutathione hydrolase, cytoplasmic isozyme (GLO2)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Zinc 1; via tele nitrogenBy similarity1
Metal bindingi61Zinc 1; via pros nitrogenBy similarity1
Metal bindingi63Zinc 2By similarity1
Metal bindingi64Zinc 2; via tele nitrogenBy similarity1
Metal bindingi121Zinc 1; via tele nitrogenBy similarity1
Metal bindingi144Zinc 1By similarity1
Metal bindingi144Zinc 2By similarity1
Binding sitei153SubstrateBy similarity1
Metal bindingi188Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YDR272W-MONOMER.
YEAST:YDR272W-MONOMER.
ReactomeiR-SCE-70268. Pyruvate metabolism.
SABIO-RKQ05584.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase, cytoplasmic isozyme (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:GLO2
Ordered Locus Names:YDR272W
ORF Names:D9954.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR272W.
SGDiS000002680. GLO2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001923461 – 274Hydroxyacylglutathione hydrolase, cytoplasmic isozymeAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei257PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05584.
PRIDEiQ05584.

PTM databases

iPTMnetiQ05584.

Interactioni

Protein-protein interaction databases

BioGridi32326. 18 interactors.
IntActiQ05584. 1 interactor.
MINTiMINT-660365.

Structurei

3D structure databases

ProteinModelPortaliQ05584.
SMRiQ05584.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 190Substrate bindingBy similarity3
Regioni268 – 271Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063033.
HOGENOMiHOG000058041.
InParanoidiQ05584.
KOiK01069.
OMAiIWTANAY.
OrthoDBiEOG092C4YMK.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR032282. HAGH_C.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVKSIKMRW ESGGVNYCYL LSDSKNKKSW LIDPAEPPEV LPELTEDEKI
60 70 80 90 100
SVEAIVNTHH HYDHADGNAD ILKYLKEKNP TSKVEVIGGS KDCPKVTIIP
110 120 130 140 150
ENLKKLHLGD LEITCIRTPC HTRDSICYYV KDPTTDERCI FTGDTLFTAG
160 170 180 190 200
CGRFFEGTGE EMDIALNNSI LETVGRQNWS KTRVYPGHEY TSDNVKFVRK
210 220 230 240 250
IYPQVGENKA LDELEQFCSK HEVTAGRFTL KDEVEFNPFM RLEDPKVQKA
260 270
AGDTNNSWDR AQIMDKLRAM KNRM
Length:274
Mass (Da):31,327
Last modified:November 1, 1996 - v1
Checksum:i6CD7AB9F0A9399EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti222E → K in AAS56065 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10292 Genomic DNA. Translation: CAA71335.1.
U51030 Genomic DNA. Translation: AAB64450.1.
AY557739 Genomic DNA. Translation: AAS56065.1.
BK006938 Genomic DNA. Translation: DAA12114.1.
PIRiS70130.
RefSeqiNP_010558.3. NM_001180580.3.

Genome annotation databases

EnsemblFungiiYDR272W; YDR272W; YDR272W.
GeneIDi851865.
KEGGisce:YDR272W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10292 Genomic DNA. Translation: CAA71335.1.
U51030 Genomic DNA. Translation: AAB64450.1.
AY557739 Genomic DNA. Translation: AAS56065.1.
BK006938 Genomic DNA. Translation: DAA12114.1.
PIRiS70130.
RefSeqiNP_010558.3. NM_001180580.3.

3D structure databases

ProteinModelPortaliQ05584.
SMRiQ05584.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32326. 18 interactors.
IntActiQ05584. 1 interactor.
MINTiMINT-660365.

PTM databases

iPTMnetiQ05584.

Proteomic databases

MaxQBiQ05584.
PRIDEiQ05584.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR272W; YDR272W; YDR272W.
GeneIDi851865.
KEGGisce:YDR272W.

Organism-specific databases

EuPathDBiFungiDB:YDR272W.
SGDiS000002680. GLO2.

Phylogenomic databases

GeneTreeiENSGT00530000063033.
HOGENOMiHOG000058041.
InParanoidiQ05584.
KOiK01069.
OMAiIWTANAY.
OrthoDBiEOG092C4YMK.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
BioCyciMetaCyc:YDR272W-MONOMER.
YEAST:YDR272W-MONOMER.
ReactomeiR-SCE-70268. Pyruvate metabolism.
SABIO-RKQ05584.

Miscellaneous databases

PROiQ05584.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR032282. HAGH_C.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2_YEAST
AccessioniPrimary (citable) accession number: Q05584
Secondary accession number(s): D6VSQ4, E9P8T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.