Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosolic iron-sulfur protein assembly protein 1

Gene

CIA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.UniRule annotation1 Publication

GO - Biological processi

  • iron-sulfur cluster assembly Source: SGD
  • small molecule metabolic process Source: Reactome
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29837-MONOMER.
ReactomeiR-SCE-2564818. Cytosolic iron-sulfur cluster assembly (yeast).
R-SCE-2564830. Cytosolic iron-sulfur cluster assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic iron-sulfur protein assembly protein 1
Gene namesi
Name:CIA1UniRule annotation
Ordered Locus Names:YDR267C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR267C.
SGDiS000002675. CIA1.

Subcellular locationi

GO - Cellular componenti

  • CIA complex Source: GO_Central
  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi127R → E: Impaired in cytosolic Fe/S protein assembly. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002538061 – 330Cytosolic iron-sulfur protein assembly protein 1Add BLAST330

Proteomic databases

MaxQBiQ05583.
PRIDEiQ05583.

Interactioni

Subunit structurei

Interacts with NAR1.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NAR1P235032EBI-32145,EBI-11864
YHR122WP388294EBI-32145,EBI-24704

Protein-protein interaction databases

BioGridi32323. 16 interactors.
DIPiDIP-1836N.
IntActiQ05583. 56 interactors.
MINTiMINT-405749.

Structurei

Secondary structure

1330
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 11Combined sources7
Beta strandi17 – 23Combined sources7
Beta strandi26 – 34Combined sources9
Beta strandi36 – 40Combined sources5
Beta strandi42 – 44Combined sources3
Beta strandi47 – 52Combined sources6
Beta strandi61 – 66Combined sources6
Beta strandi70 – 77Combined sources8
Beta strandi82 – 86Combined sources5
Beta strandi98 – 103Combined sources6
Beta strandi110 – 115Combined sources6
Beta strandi121 – 126Combined sources6
Beta strandi131 – 135Combined sources5
Beta strandi144 – 149Combined sources6
Beta strandi156 – 161Combined sources6
Beta strandi163 – 172Combined sources10
Beta strandi177 – 183Combined sources7
Beta strandi186 – 193Combined sources8
Beta strandi200 – 205Combined sources6
Beta strandi208 – 211Combined sources4
Beta strandi213 – 218Combined sources6
Beta strandi223 – 231Combined sources9
Beta strandi237 – 244Combined sources8
Beta strandi253 – 258Combined sources6
Beta strandi264 – 268Combined sources5
Beta strandi273 – 279Combined sources7
Beta strandi282 – 290Combined sources9
Turni292 – 295Combined sources4
Beta strandi298 – 303Combined sources6
Beta strandi312 – 316Combined sources5
Beta strandi319 – 325Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HESX-ray1.70X1-330[»]
ProteinModelPortaliQ05583.
SMRiQ05583.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05583.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati12 – 53WD 1Add BLAST42
Repeati56 – 95WD 2Add BLAST40
Repeati105 – 144WD 3Add BLAST40
Repeati151 – 190WD 4Add BLAST40
Repeati195 – 236WD 5Add BLAST42
Repeati248 – 286WD 6Add BLAST39
Repeati292 – 330WD 7Add BLAST39

Sequence similaritiesi

Belongs to the WD repeat CIA1 family.UniRule annotation
Contains 7 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00860000133757.
HOGENOMiHOG000208901.
InParanoidiQ05583.
OMAiWEVAGDD.
OrthoDBiEOG092C4BLN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03037. ciao1. 1 hit.
InterProiIPR028608. CIAO1/Cia1.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASINLIKSL KLYKEKIWSF DFSQGILATG STDRKIKLVS VKYDDFTLID
60 70 80 90 100
VLDETAHKKA IRSVAWRPHT SLLAAGSFDS TVSIWAKEES ADRTFEMDLL
110 120 130 140 150
AIIEGHENEV KGVAWSNDGY YLATCSRDKS VWIWETDESG EEYECISVLQ
160 170 180 190 200
EHSQDVKHVI WHPSEALLAS SSYDDTVRIW KDYDDDWECV AVLNGHEGTV
210 220 230 240 250
WSSDFDKTEG VFRLCSGSDD STVRVWKYMG DDEDDQQEWV CEAILPDVHK
260 270 280 290 300
RQVYNVAWGF NGLIASVGAD GVLAVYEEVD GEWKVFAKRA LCHGVYEINV
310 320 330
VKWLELNGKT ILATGGDDGI VNFWSLEKAA
Length:330
Mass (Da):37,275
Last modified:November 1, 1996 - v1
Checksum:iB44CCAA3125FD666
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51030 Genomic DNA. Translation: AAB64456.1.
BK006938 Genomic DNA. Translation: DAA12111.1.
PIRiS70127.
RefSeqiNP_010553.3. NM_001180575.3.

Genome annotation databases

EnsemblFungiiYDR267C; YDR267C; YDR267C.
GeneIDi851860.
KEGGisce:YDR267C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51030 Genomic DNA. Translation: AAB64456.1.
BK006938 Genomic DNA. Translation: DAA12111.1.
PIRiS70127.
RefSeqiNP_010553.3. NM_001180575.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HESX-ray1.70X1-330[»]
ProteinModelPortaliQ05583.
SMRiQ05583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32323. 16 interactors.
DIPiDIP-1836N.
IntActiQ05583. 56 interactors.
MINTiMINT-405749.

Proteomic databases

MaxQBiQ05583.
PRIDEiQ05583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR267C; YDR267C; YDR267C.
GeneIDi851860.
KEGGisce:YDR267C.

Organism-specific databases

EuPathDBiFungiDB:YDR267C.
SGDiS000002675. CIA1.

Phylogenomic databases

GeneTreeiENSGT00860000133757.
HOGENOMiHOG000208901.
InParanoidiQ05583.
OMAiWEVAGDD.
OrthoDBiEOG092C4BLN.

Enzyme and pathway databases

BioCyciYEAST:G3O-29837-MONOMER.
ReactomeiR-SCE-2564818. Cytosolic iron-sulfur cluster assembly (yeast).
R-SCE-2564830. Cytosolic iron-sulfur cluster assembly.

Miscellaneous databases

EvolutionaryTraceiQ05583.
PROiQ05583.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03037. ciao1. 1 hit.
InterProiIPR028608. CIAO1/Cia1.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIAO1_YEAST
AccessioniPrimary (citable) accession number: Q05583
Secondary accession number(s): D6VSQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5640 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.