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Reviewed, UniProtKB/Swiss-Prot Q05582 (CAS2_STRCL)

Last modified November 25, 2008. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Clavaminate synthase 2
    EC=1.14.11.21
Alternative name(s):
    Clavaminic acid synthetase 2
      Short name=CAS2
      Short name=CS2
Gene names
Name: cs2
OrganismStreptomyces clavuligerus
Taxonomic identifier1901 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

Deoxyamidinoproclavaminate + 2-oxoglutarate + O(2) = amidinoproclavaminate + succinate + CO(2).

Proclavaminate + 2-oxoglutarate + O(2) = dihydroclavaminate + succinate + CO(2) + H(2)O.

Dihydroclavaminate + 2-oxoglutarate + O(2) = clavaminate + succinate + CO(2) + H(2)O.

Cofactor

Binds 1 Fe(2+) ion per subunit By similarity.

Pathway

Antibiotic biosynthesis; clavulanic acid biosynthesis; clavulanic acid from D-glyceraldehyde 3-phosphate and L-arginine: step 3/8.

Antibiotic biosynthesis; clavulanic acid biosynthesis; clavulanic acid from D-glyceraldehyde 3-phosphate and L-arginine: step 5/8.

Antibiotic biosynthesis; clavulanic acid biosynthesis; clavulanic acid from D-glyceraldehyde 3-phosphate and L-arginine: step 6/8.

Sequence similarities

Belongs to the clavaminate synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 325324Clavaminate synthase 2
PRO_0000089323

Sites

Metal binding1451Iron By similarity
Metal binding1471Iron By similarity
Metal binding2801Iron By similarity
Binding site29412-oxoglutarate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05582-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 98C03A901B371B0E

FASTA32535,840
        10         20         30         40         50         60 
MASPIVDCTP YRDELLALAS ELPEVPRADL HGFLDEAKTL AARLPEGLAA ALDTFNAVGS 

        70         80         90        100        110        120 
EDGYLLLRGL PVDDSELPET PTSTPAPLDR KRLVMEAMRA LAGRRLGLHT GYQELRSGTV 

       130        140        150        160        170        180 
YHDVYPSPGA HYLSSETSET LLEFHTEMAY HILQPNYVML ACSRADHENR AETLVGSVRK 

       190        200        210        220        230        240 
ALPLLDEKTR ARLFDRKVPC CVDVAFRGGV DDPGAIANVK PLYGDANDPF LGYDRELLAP 

       250        260        270        280        290        300 
EDPADKEAVA HLSQALDDVT VGVKLVPGDV LIIDNFRTTH ARTPFSPRWD GKDRWLHRVY 

       310        320 
IRTDRNGELS GGERAGDTIS FSPRR 

« Hide

References

[1]"Two isozymes of clavaminate synthase central to clavulanic acid formation: cloning and sequencing of both genes from Streptomyces clavuligerus."
Marsh E.N., Chang M.D.-T., Townsend C.A.
Biochemistry 31:12648-12657(1992) [PubMed: 1472501] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-24.
Strain: ATCC 27064 / DSM 738 / IFO 13307 / JCM 4710 / NRRL 3585.
[2]"Expression and purification of two isozymes of clavaminate synthase and initial characterization of the iron binding site. General error analysis in polymerase chain reaction amplification."
Busby R.W., Chang M.D.-T., Busby R.C., Wimp J., Townsend C.A.
J. Biol. Chem. 270:4262-4269(1995) [PubMed: 7876185] [Abstract]
Cited for: CHARACTERIZATION.
Strain: ATCC 27064 / DSM 738 / IFO 13307 / JCM 4710 / NRRL 3585.

Cross-references

Sequence databases

L06214 Genomic DNA. Translation: AAA26723.1.

3D structure databases

HSSPHSSP built from PDB template 1DRY based on UniProtKB Q05581.
SMRQ05582. Positions 6-324.
ModBaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MON-13487.

Family and domain databases

InterProIPR014503. Clavaminate_syn.
IPR003819. Taurine_dOase.
[Graphical view]
PfamPF02668. TauD. 1 hit.
[Graphical view]
PIRSFPIRSF019543. Clavaminate_syn. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCAS2_STRCL
AccessionPrimary (citable) accession number: Q05582
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 23, 2007
Last modified: November 25, 2008
This is version 48 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents