Reviewed, UniProtKB/Swiss-Prot Q05582 (CAS2_STRCL)

Names and origin

Protein names

Recommended name:
    Clavaminate synthase 2
    EC=1.14.11.21
Alternative name(s):
    Clavaminic acid synthase 2
      Short name=CAS2
      Short name=CS2

Gene names

Name:

cs2

Organism

Streptomyces clavuligerus

Taxonomic identifier

1901 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2. Proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O. Dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Pathway	Antibiotic biosynthesis; clavulanate biosynthesis; clavulanate from D-glyceraldehyde 3-phosphate and L-arginine: step 3/8. Antibiotic biosynthesis; clavulanate biosynthesis; clavulanate from D-glyceraldehyde 3-phosphate and L-arginine: step 5/8. Antibiotic biosynthesis; clavulanate biosynthesis; clavulanate from D-glyceraldehyde 3-phosphate and L-arginine: step 6/8.
Sequence similarities	Belongs to the clavaminate synthase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	clavaminate synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 325	324	Clavaminate synthase 2		PRO_0000089323
Sites
	Metal binding	145	1	Iron By similarity
	Metal binding	147	1	Iron By similarity
	Metal binding	280	1	Iron By similarity
	Binding site	294	1	2-oxoglutarate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05582-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 98C03A901B371B0E	FASTA	325	35,840
	10 20 30 40 50 60 MASPIVDCTP YRDELLALAS ELPEVPRADL HGFLDEAKTL AARLPEGLAA ALDTFNAVGS 70 80 90 100 110 120 EDGYLLLRGL PVDDSELPET PTSTPAPLDR KRLVMEAMRA LAGRRLGLHT GYQELRSGTV 130 140 150 160 170 180 YHDVYPSPGA HYLSSETSET LLEFHTEMAY HILQPNYVML ACSRADHENR AETLVGSVRK 190 200 210 220 230 240 ALPLLDEKTR ARLFDRKVPC CVDVAFRGGV DDPGAIANVK PLYGDANDPF LGYDRELLAP 250 260 270 280 290 300 EDPADKEAVA HLSQALDDVT VGVKLVPGDV LIIDNFRTTH ARTPFSPRWD GKDRWLHRVY 310 320 IRTDRNGELS GGERAGDTIS FSPRR

References

[1]

"Two isozymes of clavaminate synthase central to clavulanic acid formation: cloning and sequencing of both genes from Streptomyces clavuligerus." Marsh E.N., Chang M.D.-T., Townsend C.A. Biochemistry 31:12648-12657(1992) [PubMed: 1472501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-24. Strain: ATCC 27064 / DSM 738 / IFO 13307 / JCM 4710 / NRRL 3585.

[2]

"Expression and purification of two isozymes of clavaminate synthase and initial characterization of the iron binding site. General error analysis in polymerase chain reaction amplification." Busby R.W., Chang M.D.-T., Busby R.C., Wimp J., Townsend C.A. J. Biol. Chem. 270:4262-4269(1995) [PubMed: 7876185] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 27064 / DSM 738 / IFO 13307 / JCM 4710 / NRRL 3585.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06214 Genomic DNA. Translation: AAA26723.1.
3D structure databases
SMR	Q05582. Positions 6-324.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13487.
BRENDA	1.14.11.21. 229786.
Family and domain databases
InterPro	IPR014503. Clavaminate_syn. IPR003819. Taurine_dOase. [Graphical view]
Pfam	PF02668. TauD. 2 hits. [Graphical view]
PIRSF	PIRSF019543. Clavaminate_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAS2_STRCL

Accession

Primary (citable) accession number: Q05582

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)