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Q05581

- CAS1_STRC2

UniProt

Q05581 - CAS1_STRC2

Protein

Clavaminate synthase 1

Gene

cs1

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2.
    Proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O.
    Dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O.

    Cofactori

    Binds 1 Fe2+ ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi144 – 1441Iron
    Metal bindingi146 – 1461Iron
    Metal bindingi279 – 2791Iron
    Binding sitei293 – 29312-oxoglutarateBy similarity

    GO - Molecular functioni

    1. clavaminate synthase activity Source: UniProtKB-EC
    2. iron ion binding Source: InterPro

    GO - Biological processi

    1. antibiotic biosynthetic process Source: UniProtKB-KW
    2. clavulanic acid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00112; UER00244.
    UPA00112; UER00246.
    UPA00112; UER00247.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Clavaminate synthase 1 (EC:1.14.11.21)
    Alternative name(s):
    Clavaminic acid synthase 1
    Short name:
    CAS1
    Short name:
    CS1
    Gene namesi
    Name:cs1
    OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
    Taxonomic identifieri443255 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 324323Clavaminate synthase 1PRO_0000089322Add
    BLAST

    Structurei

    Secondary structure

    1
    324
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 53
    Helixi7 – 93
    Helixi10 – 189
    Helixi24 – 274
    Helixi28 – 3912
    Helixi44 – 5613
    Beta strandi61 – 677
    Helixi73 – 753
    Beta strandi82 – 843
    Helixi93 – 10513
    Beta strandi107 – 1115
    Helixi115 – 1173
    Beta strandi119 – 1235
    Beta strandi137 – 1393
    Beta strandi141 – 1444
    Turni146 – 1494
    Beta strandi155 – 1639
    Beta strandi172 – 1765
    Helixi177 – 1804
    Helixi181 – 1833
    Helixi186 – 1927
    Beta strandi197 – 1993
    Helixi203 – 2053
    Helixi212 – 2143
    Beta strandi215 – 2184
    Beta strandi220 – 2234
    Turni234 – 2363
    Helixi242 – 25716
    Beta strandi260 – 2623
    Beta strandi269 – 2735
    Turni274 – 2763
    Beta strandi277 – 2815
    Beta strandi294 – 3018
    Helixi304 – 3063
    Beta strandi316 – 3183

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DRTX-ray2.10A1-324[»]
    1DRYX-ray1.40A1-324[»]
    1DS0X-ray1.63A1-324[»]
    1DS1X-ray1.08A1-324[»]
    1GVGX-ray1.54A1-324[»]
    ProteinModelPortaliQ05581.
    SMRiQ05581. Positions 2-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ05581.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the clavaminate synthase family.Curated

    Family and domain databases

    InterProiIPR014503. Clavaminate_syn.
    IPR003819. Taurine_dOase.
    [Graphical view]
    PfamiPF02668. TauD. 2 hits.
    [Graphical view]
    PIRSFiPIRSF019543. Clavaminate_syn. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05581-1 [UniParc]FASTAAdd to Basket

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    MTSVDCTAYG PELRALAARL PRTPRADLYA FLDAAHTAAA SLPGALATAL    50
    DTFNAEGSED GHLLLRGLPV EADADLPTTP SSTPAPEDRS LLTMEAMLGL 100
    VGRRLGLHTG YRELRSGTVY HDVYPSPGAH HLSSETSETL LEFHTEMAYH 150
    RLQPNYVMLA CSRADHERTA ATLVASVRKA LPLLDERTRA RLLDRRMPCC 200
    VDVAFRGGVD DPGAIAQVKP LYGDADDPFL GYDRELLAPE DPADKEAVAA 250
    LSKALDEVTE AVYLEPGDLL IVDNFRTTHA RTPFSPRWDG KDRWLHRVYI 300
    RTDRNGQLSG GERAGDVVAF TPRG 324
    Length:324
    Mass (Da):35,370
    Last modified:January 23, 2007 - v3
    Checksum:iF5C251134CA4933E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06213 Genomic DNA. Translation: AAA26722.1.
    PIRiA44241.
    RefSeqiWP_003961114.1. NZ_CM001015.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06213 Genomic DNA. Translation: AAA26722.1 .
    PIRi A44241.
    RefSeqi WP_003961114.1. NZ_CM001015.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DRT X-ray 2.10 A 1-324 [» ]
    1DRY X-ray 1.40 A 1-324 [» ]
    1DS0 X-ray 1.63 A 1-324 [» ]
    1DS1 X-ray 1.08 A 1-324 [» ]
    1GVG X-ray 1.54 A 1-324 [» ]
    ProteinModelPortali Q05581.
    SMRi Q05581. Positions 2-324.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00112 ; UER00244 .
    UPA00112 ; UER00246 .
    UPA00112 ; UER00247 .

    Miscellaneous databases

    EvolutionaryTracei Q05581.

    Family and domain databases

    InterProi IPR014503. Clavaminate_syn.
    IPR003819. Taurine_dOase.
    [Graphical view ]
    Pfami PF02668. TauD. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF019543. Clavaminate_syn. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Two isozymes of clavaminate synthase central to clavulanic acid formation: cloning and sequencing of both genes from Streptomyces clavuligerus."
      Marsh E.N., Chang M.D.-T., Townsend C.A.
      Biochemistry 31:12648-12657(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
      Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
    2. "Expression and purification of two isozymes of clavaminate synthase and initial characterization of the iron binding site. General error analysis in polymerase chain reaction amplification."
      Busby R.W., Chang M.D.-T., Busby R.C., Wimp J., Townsend C.A.
      J. Biol. Chem. 270:4262-4269(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
    3. "Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase."
      Zhang Z., Ren J.-S., Stammers D.K., Baldwin J.E., Harlos K., Schofield C.J.
      Nat. Struct. Biol. 7:127-133(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.08 ANGSTROMS).
      Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
    4. "Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements."
      Zhang Z., Ren J.-S., Harlos K., McKinnon C.H., Clifton I.J., Schofield C.J.
      FEBS Lett. 517:7-12(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS).
      Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

    Entry informationi

    Entry nameiCAS1_STRC2
    AccessioniPrimary (citable) accession number: Q05581
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 74 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3