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Protein

Clavaminate synthase 1

Gene

cs1

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2.
Proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O.
Dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O.

Cofactori

Fe2+Note: Binds 1 Fe2+ ion per subunit.

Pathwayi: clavulanate biosynthesis

This protein is involved in step 3, 5 and 6 of the subpathway that synthesizes clavulanate from D-glyceraldehyde 3-phosphate and L-arginine.
Proteins known to be involved in the 8 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Carboxyethyl-arginine beta-lactam-synthase (bls)
  3. Clavaminate synthase 2 (cs2), Clavaminate synthase 1 (cs1)
  4. Proclavaminate amidinohydrolase (pah)
  5. Clavaminate synthase 2 (cs2), Clavaminate synthase 1 (cs1)
  6. Clavaminate synthase 2 (cs2), Clavaminate synthase 1 (cs1)
  7. no protein annotated in this organism
  8. no protein annotated in this organism
This subpathway is part of the pathway clavulanate biosynthesis, which is itself part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes clavulanate from D-glyceraldehyde 3-phosphate and L-arginine, the pathway clavulanate biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144Iron1
Metal bindingi146Iron1
Metal bindingi279Iron1
Binding sitei2932-oxoglutarateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.11.21. 5988.
UniPathwayiUPA00112; UER00244.
UPA00112; UER00246.
UPA00112; UER00247.

Names & Taxonomyi

Protein namesi
Recommended name:
Clavaminate synthase 1 (EC:1.14.11.21)
Alternative name(s):
Clavaminic acid synthase 1
Short name:
CAS1
Short name:
CS1
Gene namesi
Name:cs1
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000893222 – 324Clavaminate synthase 1Add BLAST323

Interactioni

Protein-protein interaction databases

STRINGi443255.SclaA2_010100013974.

Structurei

Secondary structure

1324
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Helixi7 – 9Combined sources3
Helixi10 – 18Combined sources9
Helixi24 – 27Combined sources4
Helixi28 – 39Combined sources12
Helixi44 – 56Combined sources13
Beta strandi61 – 67Combined sources7
Helixi73 – 75Combined sources3
Beta strandi82 – 84Combined sources3
Helixi93 – 105Combined sources13
Beta strandi107 – 111Combined sources5
Helixi115 – 117Combined sources3
Beta strandi119 – 123Combined sources5
Beta strandi137 – 139Combined sources3
Beta strandi141 – 144Combined sources4
Turni146 – 149Combined sources4
Beta strandi155 – 163Combined sources9
Beta strandi172 – 176Combined sources5
Helixi177 – 180Combined sources4
Helixi181 – 183Combined sources3
Helixi186 – 192Combined sources7
Beta strandi197 – 199Combined sources3
Helixi203 – 205Combined sources3
Helixi212 – 214Combined sources3
Beta strandi215 – 218Combined sources4
Beta strandi220 – 223Combined sources4
Turni234 – 236Combined sources3
Helixi242 – 257Combined sources16
Beta strandi260 – 262Combined sources3
Beta strandi269 – 273Combined sources5
Turni274 – 276Combined sources3
Beta strandi277 – 281Combined sources5
Beta strandi294 – 301Combined sources8
Helixi304 – 306Combined sources3
Beta strandi316 – 318Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DRTX-ray2.10A1-324[»]
1DRYX-ray1.40A1-324[»]
1DS0X-ray1.63A1-324[»]
1DS1X-ray1.08A1-324[»]
1GVGX-ray1.54A1-324[»]
ProteinModelPortaliQ05581.
SMRiQ05581.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05581.

Family & Domainsi

Sequence similaritiesi

Belongs to the clavaminate synthase family.Curated

Family and domain databases

InterProiIPR014503. Clavaminate_syn.
IPR003819. TauD/TfdA-like.
[Graphical view]
PfamiPF02668. TauD. 2 hits.
[Graphical view]
PIRSFiPIRSF019543. Clavaminate_syn. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSVDCTAYG PELRALAARL PRTPRADLYA FLDAAHTAAA SLPGALATAL
60 70 80 90 100
DTFNAEGSED GHLLLRGLPV EADADLPTTP SSTPAPEDRS LLTMEAMLGL
110 120 130 140 150
VGRRLGLHTG YRELRSGTVY HDVYPSPGAH HLSSETSETL LEFHTEMAYH
160 170 180 190 200
RLQPNYVMLA CSRADHERTA ATLVASVRKA LPLLDERTRA RLLDRRMPCC
210 220 230 240 250
VDVAFRGGVD DPGAIAQVKP LYGDADDPFL GYDRELLAPE DPADKEAVAA
260 270 280 290 300
LSKALDEVTE AVYLEPGDLL IVDNFRTTHA RTPFSPRWDG KDRWLHRVYI
310 320
RTDRNGQLSG GERAGDVVAF TPRG
Length:324
Mass (Da):35,370
Last modified:January 23, 2007 - v3
Checksum:iF5C251134CA4933E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06213 Genomic DNA. Translation: AAA26722.1.
PIRiA44241.
RefSeqiWP_003961114.1. NZ_CM001015.1.

Genome annotation databases

KEGGiag:AAA26722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06213 Genomic DNA. Translation: AAA26722.1.
PIRiA44241.
RefSeqiWP_003961114.1. NZ_CM001015.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DRTX-ray2.10A1-324[»]
1DRYX-ray1.40A1-324[»]
1DS0X-ray1.63A1-324[»]
1DS1X-ray1.08A1-324[»]
1GVGX-ray1.54A1-324[»]
ProteinModelPortaliQ05581.
SMRiQ05581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi443255.SclaA2_010100013974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA26722.

Enzyme and pathway databases

UniPathwayiUPA00112; UER00244.
UPA00112; UER00246.
UPA00112; UER00247.
BRENDAi1.14.11.21. 5988.

Miscellaneous databases

EvolutionaryTraceiQ05581.

Family and domain databases

InterProiIPR014503. Clavaminate_syn.
IPR003819. TauD/TfdA-like.
[Graphical view]
PfamiPF02668. TauD. 2 hits.
[Graphical view]
PIRSFiPIRSF019543. Clavaminate_syn. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAS1_STRC2
AccessioniPrimary (citable) accession number: Q05581
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.