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Q05581

- CAS1_STRC2

UniProt

Q05581 - CAS1_STRC2

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Protein

Clavaminate synthase 1

Gene

cs1

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2.
Proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O.
Dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O.

Cofactori

Fe2+Note: Binds 1 Fe(2+) ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi144 – 1441Iron
Metal bindingi146 – 1461Iron
Metal bindingi279 – 2791Iron
Binding sitei293 – 29312-oxoglutarateBy similarity

GO - Molecular functioni

  1. clavaminate synthase activity Source: UniProtKB-EC
  2. iron ion binding Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
  2. clavulanic acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00112; UER00244.
UPA00112; UER00246.
UPA00112; UER00247.

Names & Taxonomyi

Protein namesi
Recommended name:
Clavaminate synthase 1 (EC:1.14.11.21)
Alternative name(s):
Clavaminic acid synthase 1
Short name:
CAS1
Short name:
CS1
Gene namesi
Name:cs1
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 324323Clavaminate synthase 1PRO_0000089322Add
BLAST

Structurei

Secondary structure

1
324
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Helixi7 – 93Combined sources
Helixi10 – 189Combined sources
Helixi24 – 274Combined sources
Helixi28 – 3912Combined sources
Helixi44 – 5613Combined sources
Beta strandi61 – 677Combined sources
Helixi73 – 753Combined sources
Beta strandi82 – 843Combined sources
Helixi93 – 10513Combined sources
Beta strandi107 – 1115Combined sources
Helixi115 – 1173Combined sources
Beta strandi119 – 1235Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi141 – 1444Combined sources
Turni146 – 1494Combined sources
Beta strandi155 – 1639Combined sources
Beta strandi172 – 1765Combined sources
Helixi177 – 1804Combined sources
Helixi181 – 1833Combined sources
Helixi186 – 1927Combined sources
Beta strandi197 – 1993Combined sources
Helixi203 – 2053Combined sources
Helixi212 – 2143Combined sources
Beta strandi215 – 2184Combined sources
Beta strandi220 – 2234Combined sources
Turni234 – 2363Combined sources
Helixi242 – 25716Combined sources
Beta strandi260 – 2623Combined sources
Beta strandi269 – 2735Combined sources
Turni274 – 2763Combined sources
Beta strandi277 – 2815Combined sources
Beta strandi294 – 3018Combined sources
Helixi304 – 3063Combined sources
Beta strandi316 – 3183Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DRTX-ray2.10A1-324[»]
1DRYX-ray1.40A1-324[»]
1DS0X-ray1.63A1-324[»]
1DS1X-ray1.08A1-324[»]
1GVGX-ray1.54A1-324[»]
ProteinModelPortaliQ05581.
SMRiQ05581. Positions 2-324.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05581.

Family & Domainsi

Sequence similaritiesi

Belongs to the clavaminate synthase family.Curated

Family and domain databases

InterProiIPR014503. Clavaminate_syn.
IPR003819. Taurine_dOase.
[Graphical view]
PfamiPF02668. TauD. 2 hits.
[Graphical view]
PIRSFiPIRSF019543. Clavaminate_syn. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05581-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSVDCTAYG PELRALAARL PRTPRADLYA FLDAAHTAAA SLPGALATAL
60 70 80 90 100
DTFNAEGSED GHLLLRGLPV EADADLPTTP SSTPAPEDRS LLTMEAMLGL
110 120 130 140 150
VGRRLGLHTG YRELRSGTVY HDVYPSPGAH HLSSETSETL LEFHTEMAYH
160 170 180 190 200
RLQPNYVMLA CSRADHERTA ATLVASVRKA LPLLDERTRA RLLDRRMPCC
210 220 230 240 250
VDVAFRGGVD DPGAIAQVKP LYGDADDPFL GYDRELLAPE DPADKEAVAA
260 270 280 290 300
LSKALDEVTE AVYLEPGDLL IVDNFRTTHA RTPFSPRWDG KDRWLHRVYI
310 320
RTDRNGQLSG GERAGDVVAF TPRG
Length:324
Mass (Da):35,370
Last modified:January 23, 2007 - v3
Checksum:iF5C251134CA4933E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06213 Genomic DNA. Translation: AAA26722.1.
PIRiA44241.
RefSeqiWP_003961114.1. NZ_CM001015.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06213 Genomic DNA. Translation: AAA26722.1 .
PIRi A44241.
RefSeqi WP_003961114.1. NZ_CM001015.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DRT X-ray 2.10 A 1-324 [» ]
1DRY X-ray 1.40 A 1-324 [» ]
1DS0 X-ray 1.63 A 1-324 [» ]
1DS1 X-ray 1.08 A 1-324 [» ]
1GVG X-ray 1.54 A 1-324 [» ]
ProteinModelPortali Q05581.
SMRi Q05581. Positions 2-324.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00112 ; UER00244 .
UPA00112 ; UER00246 .
UPA00112 ; UER00247 .

Miscellaneous databases

EvolutionaryTracei Q05581.

Family and domain databases

InterProi IPR014503. Clavaminate_syn.
IPR003819. Taurine_dOase.
[Graphical view ]
Pfami PF02668. TauD. 2 hits.
[Graphical view ]
PIRSFi PIRSF019543. Clavaminate_syn. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Two isozymes of clavaminate synthase central to clavulanic acid formation: cloning and sequencing of both genes from Streptomyces clavuligerus."
    Marsh E.N., Chang M.D.-T., Townsend C.A.
    Biochemistry 31:12648-12657(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
  2. "Expression and purification of two isozymes of clavaminate synthase and initial characterization of the iron binding site. General error analysis in polymerase chain reaction amplification."
    Busby R.W., Chang M.D.-T., Busby R.C., Wimp J., Townsend C.A.
    J. Biol. Chem. 270:4262-4269(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
  3. "Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase."
    Zhang Z., Ren J.-S., Stammers D.K., Baldwin J.E., Harlos K., Schofield C.J.
    Nat. Struct. Biol. 7:127-133(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.08 ANGSTROMS).
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
  4. "Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements."
    Zhang Z., Ren J.-S., Harlos K., McKinnon C.H., Clifton I.J., Schofield C.J.
    FEBS Lett. 517:7-12(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS).
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

Entry informationi

Entry nameiCAS1_STRC2
AccessioniPrimary (citable) accession number: Q05581
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3