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Q05581

- CAS1_STRC2

UniProt

Q05581 - CAS1_STRC2

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Protein
Clavaminate synthase 1
Gene
cs1
Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2.
Proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O.
Dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O.

Cofactori

Binds 1 Fe2+ ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi144 – 1441Iron
Metal bindingi146 – 1461Iron
Metal bindingi279 – 2791Iron
Binding sitei293 – 29312-oxoglutarate By similarity

GO - Molecular functioni

  1. clavaminate synthase activity Source: UniProtKB-EC
  2. iron ion binding Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
  2. clavulanic acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00112; UER00244.
UPA00112; UER00246.
UPA00112; UER00247.

Names & Taxonomyi

Protein namesi
Recommended name:
Clavaminate synthase 1 (EC:1.14.11.21)
Alternative name(s):
Clavaminic acid synthase 1
Short name:
CAS1
Short name:
CS1
Gene namesi
Name:cs1
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 324323Clavaminate synthase 1
PRO_0000089322Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Helixi7 – 93
Helixi10 – 189
Helixi24 – 274
Helixi28 – 3912
Helixi44 – 5613
Beta strandi61 – 677
Helixi73 – 753
Beta strandi82 – 843
Helixi93 – 10513
Beta strandi107 – 1115
Helixi115 – 1173
Beta strandi119 – 1235
Beta strandi137 – 1393
Beta strandi141 – 1444
Turni146 – 1494
Beta strandi155 – 1639
Beta strandi172 – 1765
Helixi177 – 1804
Helixi181 – 1833
Helixi186 – 1927
Beta strandi197 – 1993
Helixi203 – 2053
Helixi212 – 2143
Beta strandi215 – 2184
Beta strandi220 – 2234
Turni234 – 2363
Helixi242 – 25716
Beta strandi260 – 2623
Beta strandi269 – 2735
Turni274 – 2763
Beta strandi277 – 2815
Beta strandi294 – 3018
Helixi304 – 3063
Beta strandi316 – 3183

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DRTX-ray2.10A1-324[»]
1DRYX-ray1.40A1-324[»]
1DS0X-ray1.63A1-324[»]
1DS1X-ray1.08A1-324[»]
1GVGX-ray1.54A1-324[»]
ProteinModelPortaliQ05581.
SMRiQ05581. Positions 2-324.

Miscellaneous databases

EvolutionaryTraceiQ05581.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR014503. Clavaminate_syn.
IPR003819. Taurine_dOase.
[Graphical view]
PfamiPF02668. TauD. 2 hits.
[Graphical view]
PIRSFiPIRSF019543. Clavaminate_syn. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05581-1 [UniParc]FASTAAdd to Basket

« Hide

MTSVDCTAYG PELRALAARL PRTPRADLYA FLDAAHTAAA SLPGALATAL    50
DTFNAEGSED GHLLLRGLPV EADADLPTTP SSTPAPEDRS LLTMEAMLGL 100
VGRRLGLHTG YRELRSGTVY HDVYPSPGAH HLSSETSETL LEFHTEMAYH 150
RLQPNYVMLA CSRADHERTA ATLVASVRKA LPLLDERTRA RLLDRRMPCC 200
VDVAFRGGVD DPGAIAQVKP LYGDADDPFL GYDRELLAPE DPADKEAVAA 250
LSKALDEVTE AVYLEPGDLL IVDNFRTTHA RTPFSPRWDG KDRWLHRVYI 300
RTDRNGQLSG GERAGDVVAF TPRG 324
Length:324
Mass (Da):35,370
Last modified:January 23, 2007 - v3
Checksum:iF5C251134CA4933E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06213 Genomic DNA. Translation: AAA26722.1.
PIRiA44241.
RefSeqiWP_003961114.1. NZ_CM001015.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06213 Genomic DNA. Translation: AAA26722.1 .
PIRi A44241.
RefSeqi WP_003961114.1. NZ_CM001015.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DRT X-ray 2.10 A 1-324 [» ]
1DRY X-ray 1.40 A 1-324 [» ]
1DS0 X-ray 1.63 A 1-324 [» ]
1DS1 X-ray 1.08 A 1-324 [» ]
1GVG X-ray 1.54 A 1-324 [» ]
ProteinModelPortali Q05581.
SMRi Q05581. Positions 2-324.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00112 ; UER00244 .
UPA00112 ; UER00246 .
UPA00112 ; UER00247 .

Miscellaneous databases

EvolutionaryTracei Q05581.

Family and domain databases

InterProi IPR014503. Clavaminate_syn.
IPR003819. Taurine_dOase.
[Graphical view ]
Pfami PF02668. TauD. 2 hits.
[Graphical view ]
PIRSFi PIRSF019543. Clavaminate_syn. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Two isozymes of clavaminate synthase central to clavulanic acid formation: cloning and sequencing of both genes from Streptomyces clavuligerus."
    Marsh E.N., Chang M.D.-T., Townsend C.A.
    Biochemistry 31:12648-12657(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
  2. "Expression and purification of two isozymes of clavaminate synthase and initial characterization of the iron binding site. General error analysis in polymerase chain reaction amplification."
    Busby R.W., Chang M.D.-T., Busby R.C., Wimp J., Townsend C.A.
    J. Biol. Chem. 270:4262-4269(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
  3. "Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase."
    Zhang Z., Ren J.-S., Stammers D.K., Baldwin J.E., Harlos K., Schofield C.J.
    Nat. Struct. Biol. 7:127-133(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.08 ANGSTROMS).
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.
  4. "Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements."
    Zhang Z., Ren J.-S., Harlos K., McKinnon C.H., Clifton I.J., Schofield C.J.
    FEBS Lett. 517:7-12(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS).
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

Entry informationi

Entry nameiCAS1_STRC2
AccessioniPrimary (citable) accession number: Q05581
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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