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Protein

Sphingosine-1-phosphate lyase

Gene

DPL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine.2 Publications

Catalytic activityi

Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • calcium-mediated signaling Source: SGD
  • carboxylic acid metabolic process Source: InterPro
  • cellular response to starvation Source: SGD
  • sphingolipid metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YDR294C-MONOMER.
YEAST:YDR294C-MONOMER.
BRENDAi4.1.2.27. 984.
ReactomeiR-SCE-1660661. Sphingolipid de novo biosynthesis.
UniPathwayiUPA00222.

Chemistry databases

SwissLipidsiSLP:000000071.
SLP:000000196.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine-1-phosphate lyase (EC:4.1.2.27)
Short name:
S1PL
Short name:
SP-lyase
Short name:
ySPL
Alternative name(s):
Bestowed of sphingosine tolerance 1
Sphingosine-1-phosphate aldolase
Gene namesi
Name:DPL1
Synonyms:BST1
Ordered Locus Names:YDR294C
ORF Names:D9819.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR294C.
SGDiS000002702. DPL1.

Subcellular locationi

GO - Cellular componenti

  • cortical endoplasmic reticulum Source: SGD
  • perinuclear endoplasmic reticulum Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi172A → P: Loss of enzyme activity. 1 Publication1
Mutagenesisi174Y → F: Mildly decreased enzyme activity. 1 Publication1
Mutagenesisi198H → A: Decreased enzyme activity. 1 Publication1
Mutagenesisi380K → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi386K → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi554Y → F: Decreased enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001470181 – 589Sphingosine-1-phosphate lyaseAdd BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei380N6-(pyridoxal phosphate)lysine1

Proteomic databases

MaxQBiQ05567.
PRIDEiQ05567.

PTM databases

iPTMnetiQ05567.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi32346. 47 interactors.
DIPiDIP-49371N.
IntActiQ05567. 2 interactors.
MINTiMINT-4481843.

Structurei

Secondary structure

1589
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi145 – 157Combined sources13
Helixi164 – 166Combined sources3
Beta strandi169 – 172Combined sources4
Helixi178 – 190Combined sources13
Turni199 – 201Combined sources3
Helixi203 – 219Combined sources17
Turni224 – 226Combined sources3
Beta strandi229 – 234Combined sources6
Helixi235 – 253Combined sources19
Beta strandi260 – 264Combined sources5
Helixi269 – 277Combined sources9
Beta strandi281 – 285Combined sources5
Turni289 – 291Combined sources3
Turni296 – 299Combined sources4
Helixi300 – 302Combined sources3
Beta strandi305 – 313Combined sources9
Turni317 – 319Combined sources3
Turni326 – 330Combined sources5
Helixi331 – 334Combined sources4
Beta strandi339 – 342Combined sources4
Turni343 – 346Combined sources4
Helixi347 – 350Combined sources4
Helixi351 – 353Combined sources3
Turni354 – 357Combined sources4
Beta strandi373 – 377Combined sources5
Turni378 – 382Combined sources5
Beta strandi389 – 392Combined sources4
Helixi396 – 399Combined sources4
Turni400 – 402Combined sources3
Beta strandi417 – 419Combined sources3
Helixi425 – 463Combined sources39
Beta strandi476 – 482Combined sources7
Turni484 – 487Combined sources4
Helixi488 – 496Combined sources9
Turni497 – 499Combined sources3
Beta strandi511 – 514Combined sources4
Turni517 – 520Combined sources4
Helixi523 – 537Combined sources15
Helixi567 – 575Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MC6X-ray3.15A/C103-589[»]
ProteinModelPortaliQ05567.
SMRiQ05567.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05567.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000000046.
HOGENOMiHOG000190693.
InParanoidiQ05567.
KOiK01634.
OMAiACTLFHG.
OrthoDBiEOG092C1H4E.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGVSNKTVS INGWYGMPIH LLREEGDFAQ FMILTINELK IAIHGYLRNT
60 70 80 90 100
PWYNMLKDYL FVIFCYKLIS NFFYLLKVYG PVRLAVRTYE HSSRRLFRWL
110 120 130 140 150
LDSPFLRGTV EKEVTKVKQS IEDELIRSDS QLMNFPQLPS NGIPQDDVIE
160 170 180 190 200
ELNKLNDLIP HTQWKEGKVS GAVYHGGDDL IHLQTIAYEK YCVANQLHPD
210 220 230 240 250
VFPAVRKMES EVVSMVLRMF NAPSDTGCGT TTSGGTESLL LACLSAKMYA
260 270 280 290 300
LHHRGITEPE IIAPVTAHAG FDKAAYYFGM KLRHVELDPT TYQVDLGKVK
310 320 330 340 350
KFINKNTILL VGSAPNFPHG IADDIEGLGK IAQKYKLPLH VDSCLGSFIV
360 370 380 390 400
SFMEKAGYKN LPLLDFRVPG VTSISCDTHK YGFAPKGSSV IMYRNSDLRM
410 420 430 440 450
HQYYVNPAWT GGLYGSPTLA GSRPGAIVVG CWATMVNMGE NGYIESCQEI
460 470 480 490 500
VGAAMKFKKY IQENIPDLNI MGNPRYSVIS FSSKTLNIHE LSDRLSKKGW
510 520 530 540 550
HFNALQKPVA LHMAFTRLSA HVVDEICDIL RTTVQELKSE SNSKPSPDGT
560 570 580
SALYGVAGSV KTAGVADKLI VGFLDALYKL GPGEDTATK
Length:589
Mass (Da):65,566
Last modified:November 1, 1996 - v1
Checksum:i75FAF8182AF72266
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51031 Genomic DNA. Translation: AAB64470.1.
BK006938 Genomic DNA. Translation: DAA12133.1.
PIRiS70123.
RefSeqiNP_010580.1. NM_001180602.1.

Genome annotation databases

EnsemblFungiiYDR294C; YDR294C; YDR294C.
GeneIDi851888.
KEGGisce:YDR294C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51031 Genomic DNA. Translation: AAB64470.1.
BK006938 Genomic DNA. Translation: DAA12133.1.
PIRiS70123.
RefSeqiNP_010580.1. NM_001180602.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MC6X-ray3.15A/C103-589[»]
ProteinModelPortaliQ05567.
SMRiQ05567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32346. 47 interactors.
DIPiDIP-49371N.
IntActiQ05567. 2 interactors.
MINTiMINT-4481843.

Chemistry databases

SwissLipidsiSLP:000000071.
SLP:000000196.

PTM databases

iPTMnetiQ05567.

Proteomic databases

MaxQBiQ05567.
PRIDEiQ05567.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR294C; YDR294C; YDR294C.
GeneIDi851888.
KEGGisce:YDR294C.

Organism-specific databases

EuPathDBiFungiDB:YDR294C.
SGDiS000002702. DPL1.

Phylogenomic databases

GeneTreeiENSGT00390000000046.
HOGENOMiHOG000190693.
InParanoidiQ05567.
KOiK01634.
OMAiACTLFHG.
OrthoDBiEOG092C1H4E.

Enzyme and pathway databases

UniPathwayiUPA00222.
BioCyciMetaCyc:YDR294C-MONOMER.
YEAST:YDR294C-MONOMER.
BRENDAi4.1.2.27. 984.
ReactomeiR-SCE-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

EvolutionaryTraceiQ05567.
PROiQ05567.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSGPL_YEAST
AccessioniPrimary (citable) accession number: Q05567
Secondary accession number(s): D6VSS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.