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Protein

Cytochrome P450 2A11

Gene

CYP2A11

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxygenation of a variety of substrates, including ethanol and procarcinogens such as N-nitrosodiethylamine and phenacetin. Has no or little activity as a coumarin 7-hydroxylase and in the formation of androstenedione from testosterone.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A11 (EC:1.14.14.1)
Alternative name(s):
CYPIIA11
Cytochrome P450-IIA11
Gene namesi
Name:CYP2A11
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi62 – 632RD → SE: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with L-104; V-117 and S-120. 1 Publication
Mutagenesisi104 – 1041Q → L: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; V-117 and S-120. 1 Publication
Mutagenesisi117 – 1171A → V: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; L-104 and S-120. 1 Publication
Mutagenesisi120 – 1201T → S: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; L-104 and V-117. 1 Publication
Mutagenesisi372 – 3721R → H: Significant increase in the rate of hydroxylation of testosterone, but not of coumarin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Cytochrome P450 2A11PRO_0000051674Add
BLAST

Expressioni

Tissue specificityi

Expressed in liver and lung as well as in nasal tissues.

Structurei

3D structure databases

ProteinModelPortaliQ05556.
SMRiQ05556. Positions 31-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG015789.
InParanoidiQ05556.
KOiK17685.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASGLLLAA LLACLTVMIL LSVWRQRKLW GKLPPGPTPL PFIGNYLQLN
60 70 80 90 100
TEQMYDSLMK IRDRYGPVFT IHLGPRRIVV LCGQEAVKEA LVDQAEDFSG
110 120 130 140 150
RGEQATFDWL FKGYGVAFST WERARPLRRF AISTLRDFGV GKRGIEERIQ
160 170 180 190 200
EEAGFLIEAF RDTRGAFIDP TFFLSRTVSN VISSIVFGDR FDYEDKEFLS
210 220 230 240 250
LLRMMLGSFQ FTATPTGQLY EMFYSVMKHL PGPQQQAFKE LEGLRDFIAK
260 270 280 290 300
KVERNQRTLD PNSPRDFIDS FLIRMQEEKK DPKSEFHMKN LVLTTLNLFF
310 320 330 340 350
AGTETVSTTM RYGFLLLMKH PDVEAKVHEE IDRVIGRNRQ PKFEDRAKMP
360 370 380 390 400
YTEAVIHEIQ RFTDMIPMGL ARRVTRDTKF RDFLLPKGTE VFPMLGSVLK
410 420 430 440 450
DPKFFSKPRE FYPQHFLDEK GQFKKSDAFM PFSVGKRYCL GEGLARMELF
460 470 480 490
LFFTTIMQNF RFRSQQAPQD IDVSPKHVGF ATIPRTYTMS FVPR
Length:494
Mass (Da):57,277
Last modified:June 1, 1994 - v1
Checksum:i28D2E5C4E5D0861A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10237 mRNA. Translation: AAA31372.1.
PIRiB47494.
RefSeqiNP_001164519.1. NM_001171048.1.
UniGeneiOcu.1915.

Genome annotation databases

GeneIDi100328596.
KEGGiocu:100328596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10237 mRNA. Translation: AAA31372.1.
PIRiB47494.
RefSeqiNP_001164519.1. NM_001171048.1.
UniGeneiOcu.1915.

3D structure databases

ProteinModelPortaliQ05556.
SMRiQ05556. Positions 31-494.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100328596.
KEGGiocu:100328596.

Organism-specific databases

CTDi100328596.

Phylogenomic databases

HOVERGENiHBG015789.
InParanoidiQ05556.
KOiK17685.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and heterologous expression of cloned cDNAs for two rabbit nasal microsomal proteins, CYP2A10 and CYP2A11, that are related to nasal microsomal cytochrome P450 form a."
    Peng H.-M., Ding X., Coon M.J.
    J. Biol. Chem. 268:17253-17260(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: New Zealand white.
    Tissue: Nasal mucosa.
  2. "Structure-function analysis of CYP2A10 and CYP2A11, P450 cytochromes that differ in only eight amino acids but have strikingly different activities toward testosterone and coumarin."
    Ding X., Peng H.-M., Coon M.J.
    Biochem. Biophys. Res. Commun. 203:373-378(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF 62-ARG-ASP-63; GLN-104; ALA-117; THR-120 AND ARG-372.

Entry informationi

Entry nameiCP2AB_RABIT
AccessioniPrimary (citable) accession number: Q05556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 4, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.