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Q05556 (CP2AB_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2A11

EC=1.14.14.1
Alternative name(s):
CYPIIA11
Cytochrome P450-IIA11
Gene names
Name:CYP2A11
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxygenation of a variety of substrates, including ethanol and procarcinogens such as N-nitrosodiethylamine and phenacetin. Has no or little activity as a coumarin 7-hydroxylase and in the formation of androstenedione from testosterone.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Expressed in liver and lung as well as in nasal tissues.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Cytochrome P450 2A11
PRO_0000051674

Sites

Metal binding4391Iron (heme axial ligand) By similarity

Experimental info

Mutagenesis62 – 632RD → SE: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with L-104; V-117 and S-120.
Mutagenesis1041Q → L: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; V-117 and S-120. Ref.2
Mutagenesis1171A → V: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; L-104 and S-120. Ref.2
Mutagenesis1201T → S: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; L-104 and V-117. Ref.2
Mutagenesis3721R → H: Significant increase in the rate of hydroxylation of testosterone, but not of coumarin. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q05556 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 28D2E5C4E5D0861A

FASTA49457,277
        10         20         30         40         50         60 
MLASGLLLAA LLACLTVMIL LSVWRQRKLW GKLPPGPTPL PFIGNYLQLN TEQMYDSLMK 

        70         80         90        100        110        120 
IRDRYGPVFT IHLGPRRIVV LCGQEAVKEA LVDQAEDFSG RGEQATFDWL FKGYGVAFST 

       130        140        150        160        170        180 
WERARPLRRF AISTLRDFGV GKRGIEERIQ EEAGFLIEAF RDTRGAFIDP TFFLSRTVSN 

       190        200        210        220        230        240 
VISSIVFGDR FDYEDKEFLS LLRMMLGSFQ FTATPTGQLY EMFYSVMKHL PGPQQQAFKE 

       250        260        270        280        290        300 
LEGLRDFIAK KVERNQRTLD PNSPRDFIDS FLIRMQEEKK DPKSEFHMKN LVLTTLNLFF 

       310        320        330        340        350        360 
AGTETVSTTM RYGFLLLMKH PDVEAKVHEE IDRVIGRNRQ PKFEDRAKMP YTEAVIHEIQ 

       370        380        390        400        410        420 
RFTDMIPMGL ARRVTRDTKF RDFLLPKGTE VFPMLGSVLK DPKFFSKPRE FYPQHFLDEK 

       430        440        450        460        470        480 
GQFKKSDAFM PFSVGKRYCL GEGLARMELF LFFTTIMQNF RFRSQQAPQD IDVSPKHVGF 

       490 
ATIPRTYTMS FVPR 

« Hide

References

[1]"Isolation and heterologous expression of cloned cDNAs for two rabbit nasal microsomal proteins, CYP2A10 and CYP2A11, that are related to nasal microsomal cytochrome P450 form a."
Peng H.-M., Ding X., Coon M.J.
J. Biol. Chem. 268:17253-17260(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: New Zealand white.
Tissue: Nasal mucosa.
[2]"Structure-function analysis of CYP2A10 and CYP2A11, P450 cytochromes that differ in only eight amino acids but have strikingly different activities toward testosterone and coumarin."
Ding X., Peng H.-M., Coon M.J.
Biochem. Biophys. Res. Commun. 203:373-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS OF 62-ARG-ASP-63; GLN-104; ALA-117; THR-120 AND ARG-372.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10237 mRNA. Translation: AAA31372.1.
PIRB47494.
RefSeqNP_001164519.1. NM_001171048.1.
UniGeneOcu.1915.

3D structure databases

ProteinModelPortalQ05556.
SMRQ05556. Positions 31-494.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100328596.

Organism-specific databases

CTD100328596.

Phylogenomic databases

HOVERGENHBG015789.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP2AB_RABIT
AccessionPrimary (citable) accession number: Q05556
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: December 11, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families