Q05556 (CP2AB_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2A11
EC=1.14.14.1

Alternative name(s):
CYPIIA11
Cytochrome P450-IIA11

Gene names

Name:

CYP2A11

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxygenation of a variety of substrates, including ethanol and procarcinogens such as N-nitrosodiethylamine and phenacetin. Has no or little activity as a coumarin 7-hydroxylase and in the formation of androstenedione from testosterone.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Expressed in liver and lung as well as in nasal tissues.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Cytochrome P450 2A11		PRO_0000051674
Sites
	Metal binding	439	1	Iron (heme axial ligand) By similarity
Experimental info
	Mutagenesis	62 – 63	2	RD → SE: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with L-104; V-117 and S-120.
	Mutagenesis	104	1	Q → L: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; V-117 and S-120. Ref.2
	Mutagenesis	117	1	A → V: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; L-104 and S-120. Ref.2
	Mutagenesis	120	1	T → S: No effect. Almost as active as CYP2A10 in coumarin hydroxylation and approximately half as active as CYP2A10 in androstenedione formation; when associated with 62-SE-63; L-104 and V-117. Ref.2
	Mutagenesis	372	1	R → H: Significant increase in the rate of hydroxylation of testosterone, but not of coumarin. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q05556 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 28D2E5C4E5D0861A	FASTA	494	57,277
	10 20 30 40 50 60 MLASGLLLAA LLACLTVMIL LSVWRQRKLW GKLPPGPTPL PFIGNYLQLN TEQMYDSLMK 70 80 90 100 110 120 IRDRYGPVFT IHLGPRRIVV LCGQEAVKEA LVDQAEDFSG RGEQATFDWL FKGYGVAFST 130 140 150 160 170 180 WERARPLRRF AISTLRDFGV GKRGIEERIQ EEAGFLIEAF RDTRGAFIDP TFFLSRTVSN 190 200 210 220 230 240 VISSIVFGDR FDYEDKEFLS LLRMMLGSFQ FTATPTGQLY EMFYSVMKHL PGPQQQAFKE 250 260 270 280 290 300 LEGLRDFIAK KVERNQRTLD PNSPRDFIDS FLIRMQEEKK DPKSEFHMKN LVLTTLNLFF 310 320 330 340 350 360 AGTETVSTTM RYGFLLLMKH PDVEAKVHEE IDRVIGRNRQ PKFEDRAKMP YTEAVIHEIQ 370 380 390 400 410 420 RFTDMIPMGL ARRVTRDTKF RDFLLPKGTE VFPMLGSVLK DPKFFSKPRE FYPQHFLDEK 430 440 450 460 470 480 GQFKKSDAFM PFSVGKRYCL GEGLARMELF LFFTTIMQNF RFRSQQAPQD IDVSPKHVGF 490 ATIPRTYTMS FVPR

References

[1]	"Isolation and heterologous expression of cloned cDNAs for two rabbit nasal microsomal proteins, CYP2A10 and CYP2A11, that are related to nasal microsomal cytochrome P450 form a." Peng H.-M., Ding X., Coon M.J. J. Biol. Chem. 268:17253-17260(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Nasal mucosa.
[2]	"Structure-function analysis of CYP2A10 and CYP2A11, P450 cytochromes that differ in only eight amino acids but have strikingly different activities toward testosterone and coumarin." Ding X., Peng H.-M., Coon M.J. Biochem. Biophys. Res. Commun. 203:373-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF 62-ARG-ASP-63; GLN-104; ALA-117; THR-120 AND ARG-372.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10237 mRNA. Translation: AAA31372.1.
PIR	B47494.
RefSeq	NP_001164519.1. NM_001171048.1.
UniGene	Ocu.1915.
3D structure databases
ProteinModelPortal	Q05556.
SMR	Q05556. Positions 31-494.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100328596.
Organism-specific databases
CTD	100328596.
Phylogenomic databases
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2AB_RABIT

Accession

Primary (citable) accession number: Q05556

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program