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Protein

ATP-dependent helicase HRQ1

Gene

HRQ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Helicase with 3'-5' helicase activity involved in genome stability (PubMed:23456718, PubMed:24440721, PubMed:24700328). Functions in the RAD4-dependent nucleotide excision repair (NER) pathway and plays a critical role in DNA interstrand cross-link repair (PubMed:24440721, PubMed:24682993). Unwinds relatively long duplex DNA up to 120-bp and requires a long 3'-tail of at least 70 nucleotides for efficient unwinding of duplex DNA (PubMed:23026052). Activity is significantly stimulated by a preexisting fork structure (PubMed:24682993). Shows both processive helicase and DNA strand annealing activities (PubMed:24682993). Affects telomere length by a non-catalytic mechanism, probably through inhibiting telomerase by competing with it for ssDNA binding (PubMed:24440721).5 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.2 Publications

Cofactori

Mg2+1 Publication

pH dependencei

Optimum pH is 6.0-8.0.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi312 – 319ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: SGD
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA duplex unwinding Source: SGD
  • DNA strand renaturation Source: SGD
  • nucleotide-excision repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Stress response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29854-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase HRQ1Curated (EC:3.6.4.122 Publications)
Alternative name(s):
Homologous to recQ protein 11 Publication
Gene namesi
Name:HRQ11 Publication
Ordered Locus Names:YDR291WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR291W.
SGDiS000002699. HRQ1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to increased mitotic recombination and spontaneous mutation, as well as to sensitivity to 4-nitroquinoline 1-oxide and cisplatin (PubMed:23456718). Causes also hypersensitivity to DNA interstrand cross-links (ICLs) and telomere addition to DNA breaks (PubMed:24440721).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi318K → A: Impairs helicase and ATPase activities. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002538131 – 1077ATP-dependent helicase HRQ1Add BLAST1077

Proteomic databases

MaxQBiQ05549.
PRIDEiQ05549.

PTM databases

iPTMnetiQ05549.

Expressioni

Inductioni

By heat shock.1 Publication

Interactioni

Subunit structurei

Forms heptamer rings (PubMed:24440721). Interacts with RAD4 (PubMed:24682993).2 Publications

Protein-protein interaction databases

BioGridi32343. 135 interactors.
IntActiQ05549. 1 interactor.
MINTiMINT-2493111.

Structurei

3D structure databases

ProteinModelPortaliQ05549.
SMRiQ05549.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini299 – 483Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST185
Domaini521 – 678Helicase C-terminalPROSITE-ProRule annotationAdd BLAST158

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi423 – 426DEAH box4

Sequence similaritiesi

Belongs to the helicase family. HRQ1 subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000163591.
InParanoidiQ05549.
KOiK06877.
OMAiCELLMKE.
OrthoDBiEOG092C0E4A.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR018973. DEAD/DEAH-box_helicase.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF09369. DUF1998. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05549-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEGPIKKKL KSAGQGSGKT DAFRNFEQFF FRLNTLYTFL ICRKHVVPTF
60 70 80 90 100
KTLCGPIETA LKRTVTKEDL AMVMALMPRE CVFKYIDENQ IYTETKIFDF
110 120 130 140 150
NNGGFQQKEN DIFELKDVDD QNQTQKSTQL LIFEFIDGTM QRSWSASDRF
160 170 180 190 200
SQIKIPTYTT EEMKKMISKR EALFKSRLRE FILEKEKANL DPFSELTNLA
210 220 230 240 250
QKYIPRERDY EDPIEAMMKA KQESNEMSIP NYSNNSVITT IPQMIEKLKS
260 270 280 290 300
TEFYASQIKH CFTIPSRTAK YKGLCFELAP EVYQGMEHEN FYSHQADAIN
310 320 330 340 350
SLHQGENVII TTSTSSGKSL IYQLAAIDLL LKDPESTFMY IFPTKALAQD
360 370 380 390 400
QKRAFKVILS KIPELKNAVV DTYDGDTEPE ERAYIRKNAR VIFTNPDMIH
410 420 430 440 450
TSILPNHANW RHFLYHLKLV VVDELHIYKG LFGSHVALVM RRLLRLCHCF
460 470 480 490 500
YENSGLQFIS CSATLKSPVQ HMKDMFGINE VTLIHEDGSP TGAKHLVVWN
510 520 530 540 550
PPILPQHERK RENFIRESAK ILVQLILNNV RTIAFCYVRR VCELLMKEVR
560 570 580 590 600
NIFIETGRED LVTEVMSYRG GYSASDRRKI EREMFHGNLK AVISTNALEL
610 620 630 640 650
GIDIGGLDAV LMCGFPLSMA NFHQQSGRAG RRNNDSLTLV VASDSPVDQH
660 670 680 690 700
YVAHPESLLE VNNFESYQDL VLDFNNILIL EGHIQCAAFE LPINFERDKQ
710 720 730 740 750
YFTESHLRKI CVERLHHNQD GYHASNRFLP WPSKCVSLRG GEEDQFAVVD
760 770 780 790 800
ITNGRNIIIE EIEASRTSFT LYDGGIFIHQ GYPYLVKEFN PDERYAKVQR
810 820 830 840 850
VDVDWVTNQR DFTDVDPQEI ELIRSLRNSD VPVYFGKIKT TIIVFGFFKV
860 870 880 890 900
DKYKRIIDAI ETHNPPVIIN SKGLWIDMPK YALEICQKKQ LNVAGAIHGA
910 920 930 940 950
QHAIMGMLPR FIVAGVDEIQ TECKAPEKEF AERQTKRKRP ARLIFYDSKG
960 970 980 990 1000
GKYGSGLCVK AFEHIDDIIE SSLRRIEECP CSDGCPDCVA ASFCKENSLV
1010 1020 1030 1040 1050
LSKPGAQVVL HCILGHSEDS FIDLIKDGPE PNMPEIKVET VIPVSEHVNF
1060 1070
SDDFKIIDVR RATKDDTHTN EIIKKEI
Length:1,077
Mass (Da):123,550
Last modified:November 1, 1996 - v1
Checksum:i948F024154FBE9A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51031 Genomic DNA. Translation: AAB64466.1.
BK006938 Genomic DNA. Translation: DAA12130.1.
PIRiS70120.
RefSeqiNP_010577.3. NM_001180599.3.

Genome annotation databases

EnsemblFungiiYDR291W; YDR291W; YDR291W.
GeneIDi851885.
KEGGisce:YDR291W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51031 Genomic DNA. Translation: AAB64466.1.
BK006938 Genomic DNA. Translation: DAA12130.1.
PIRiS70120.
RefSeqiNP_010577.3. NM_001180599.3.

3D structure databases

ProteinModelPortaliQ05549.
SMRiQ05549.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32343. 135 interactors.
IntActiQ05549. 1 interactor.
MINTiMINT-2493111.

PTM databases

iPTMnetiQ05549.

Proteomic databases

MaxQBiQ05549.
PRIDEiQ05549.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR291W; YDR291W; YDR291W.
GeneIDi851885.
KEGGisce:YDR291W.

Organism-specific databases

EuPathDBiFungiDB:YDR291W.
SGDiS000002699. HRQ1.

Phylogenomic databases

HOGENOMiHOG000163591.
InParanoidiQ05549.
KOiK06877.
OMAiCELLMKE.
OrthoDBiEOG092C0E4A.

Enzyme and pathway databases

BioCyciYEAST:G3O-29854-MONOMER.

Miscellaneous databases

PROiQ05549.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR018973. DEAD/DEAH-box_helicase.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF09369. DUF1998. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHRQ1_YEAST
AccessioniPrimary (citable) accession number: Q05549
Secondary accession number(s): D6VSS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.