Q05547 (PP13_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase alpha-3 isoform
EC=3.1.3.16

Gene names

Name:	Pp1-13C
ORF Names:	CG9156

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neurogenesis Inferred from mutant phenotype PubMed 21549331. Source: FlyBase protein dephosphorylation Inferred from direct assay Ref.1. Source: FlyBase sperm competition Inferred from mutant phenotype PubMed 21716718. Source: FlyBase
Cellular_component	protein phosphatase type 1 complex Inferred from sequence or structural similarity Ref.1. Source: FlyBase
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay Ref.1. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ppi20	Q0E9G3	2	EBI-157144,EBI-150380

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Serine/threonine-protein phosphatase alpha-3 isoform		PRO_0000058793
Sites
	Active site	123	1	Proton donor By similarity
	Metal binding	62	1	Iron By similarity
	Metal binding	64	1	Iron By similarity
	Metal binding	90	1	Iron By similarity
	Metal binding	90	1	Manganese By similarity
	Metal binding	122	1	Manganese By similarity
	Metal binding	171	1	Manganese By similarity
	Metal binding	246	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05547 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: B01ACC651F5DAE14	FASTA	302	34,558
	10 20 30 40 50 60 MAEVLNLESI ISRLLEVRGA RPGKNVQLSE GEIRGLCLKS REILLAQPIL LELEAPLKIC 70 80 90 100 110 120 GDIHGQYYDL LRLFEYGGYP PEANYLFLGD YVDRGKQSLE TICLLLAYKI KYSENFFLLR 130 140 150 160 170 180 GNHECASINR IYGFYDECKR RYTIKLWKTF TDCFNCLPVV AIVDEKIFCC HGGLSPDLTS 190 200 210 220 230 240 MEQIRRIMRP TDVPDQGLLC DLLWSDPDKD TIGWGENDRG VSFTFGAEVV VKFLQKHDLD 250 260 270 280 290 300 LICRAHQVVE DGYEFFAKRQ LVTLFSAPNY CGEFDNAGAM MSVDNTLMCS FQILKPVEKR KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the fourth functional gene for protein phosphatase 1 in Drosophila melanogaster from its chromosomal location." Dombradi V., Mann D.J., Saunders R.D., Cohen P.T.W. Eur. J. Biochem. 212:177-183(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69974 Genomic DNA. Translation: CAA49594.1. AE014298 Genomic DNA. Translation: AAF48448.1. AY060272 mRNA. Translation: AAL25311.1.
PIR	S29396.
RefSeq	NP_524921.1. NM_080182.3.
UniGene	Dm.1527.
3D structure databases
ProteinModelPortal	Q05547.
SMR	Q05547. Positions 5-296.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-23332N.
IntAct	Q05547. 25 interactions.
MINT	MINT-873916.
STRING	7227.FBpp0073834.
Proteomic databases
PaxDb	Q05547.
PRIDE	Q05547.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074017; FBpp0073834; FBgn0003132.
GeneID	48531.
KEGG	dme:Dmel_CG9156.
Organism-specific databases
CTD	48531.
FlyBase	FBgn0003132. Pp1-13C.
Phylogenomic databases
eggNOG	COG0639.
GeneTree	ENSGT00530000062911.
InParanoid	Q05547.
KO	K06269.
OMA	ADKKLYP.
OrthoDB	EOG4V41QC.
PhylomeDB	Q05547.
Gene expression databases
Bgee	Q05547.
GermOnline	CG9156. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	48531.
NextBio	839441.

Entry information

Entry name

PP13_DROME

Accession

Primary (citable) accession number: Q05547
Secondary accession number(s): Q9VXW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project