Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q05547

- PP13_DROME

UniProt

Q05547 - PP13_DROME

Protein

Serine/threonine-protein phosphatase alpha-3 isoform

Gene

Pp1-13C

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Manganese 1By similarity
    Metal bindingi64 – 641Manganese 1By similarity
    Metal bindingi90 – 901Manganese 1By similarity
    Metal bindingi90 – 901Manganese 2By similarity
    Metal bindingi122 – 1221Manganese 2By similarity
    Active sitei123 – 1231Proton donorBy similarity
    Metal bindingi171 – 1711Manganese 2By similarity
    Metal bindingi246 – 2461Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: FlyBase

    GO - Biological processi

    1. neurogenesis Source: FlyBase
    2. protein dephosphorylation Source: FlyBase
    3. sperm competition Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    SignaLinkiQ05547.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase alpha-3 isoform (EC:3.1.3.16)
    Gene namesi
    Name:Pp1-13C
    ORF Names:CG9156
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0003132. Pp1-13C.

    Subcellular locationi

    GO - Cellular componenti

    1. protein phosphatase type 1 complex Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 302302Serine/threonine-protein phosphatase alpha-3 isoformPRO_0000058793Add
    BLAST

    Proteomic databases

    PaxDbiQ05547.
    PRIDEiQ05547.

    Expressioni

    Gene expression databases

    BgeeiQ05547.

    Interactioni

    Subunit structurei

    Interacts with Nop17l.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Nop17lQ0E9G32EBI-157144,EBI-150380

    Protein-protein interaction databases

    BioGridi71473. 7 interactions.
    DIPiDIP-23332N.
    IntActiQ05547. 25 interactions.
    MINTiMINT-873916.
    STRINGi7227.FBpp0073834.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05547.
    SMRiQ05547. Positions 5-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000062911.
    InParanoidiQ05547.
    KOiK06269.
    OMAiPRSMITK.
    OrthoDBiEOG7TJ3K3.
    PhylomeDBiQ05547.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q05547-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEVLNLESI ISRLLEVRGA RPGKNVQLSE GEIRGLCLKS REILLAQPIL    50
    LELEAPLKIC GDIHGQYYDL LRLFEYGGYP PEANYLFLGD YVDRGKQSLE 100
    TICLLLAYKI KYSENFFLLR GNHECASINR IYGFYDECKR RYTIKLWKTF 150
    TDCFNCLPVV AIVDEKIFCC HGGLSPDLTS MEQIRRIMRP TDVPDQGLLC 200
    DLLWSDPDKD TIGWGENDRG VSFTFGAEVV VKFLQKHDLD LICRAHQVVE 250
    DGYEFFAKRQ LVTLFSAPNY CGEFDNAGAM MSVDNTLMCS FQILKPVEKR 300
    KK 302
    Length:302
    Mass (Da):34,558
    Last modified:February 1, 1996 - v1
    Checksum:iB01ACC651F5DAE14
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69974 Genomic DNA. Translation: CAA49594.1.
    AE014298 Genomic DNA. Translation: AAF48448.1.
    AY060272 mRNA. Translation: AAL25311.1.
    PIRiS29396.
    RefSeqiNP_524921.1. NM_080182.3.
    UniGeneiDm.1527.

    Genome annotation databases

    EnsemblMetazoaiFBtr0074017; FBpp0073834; FBgn0003132.
    GeneIDi48531.
    KEGGidme:Dmel_CG9156.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69974 Genomic DNA. Translation: CAA49594.1 .
    AE014298 Genomic DNA. Translation: AAF48448.1 .
    AY060272 mRNA. Translation: AAL25311.1 .
    PIRi S29396.
    RefSeqi NP_524921.1. NM_080182.3.
    UniGenei Dm.1527.

    3D structure databases

    ProteinModelPortali Q05547.
    SMRi Q05547. Positions 5-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 71473. 7 interactions.
    DIPi DIP-23332N.
    IntActi Q05547. 25 interactions.
    MINTi MINT-873916.
    STRINGi 7227.FBpp0073834.

    Proteomic databases

    PaxDbi Q05547.
    PRIDEi Q05547.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0074017 ; FBpp0073834 ; FBgn0003132 .
    GeneIDi 48531.
    KEGGi dme:Dmel_CG9156.

    Organism-specific databases

    CTDi 48531.
    FlyBasei FBgn0003132. Pp1-13C.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000062911.
    InParanoidi Q05547.
    KOi K06269.
    OMAi PRSMITK.
    OrthoDBi EOG7TJ3K3.
    PhylomeDBi Q05547.

    Enzyme and pathway databases

    SignaLinki Q05547.

    Miscellaneous databases

    GenomeRNAii 48531.
    NextBioi 839441.

    Gene expression databases

    Bgeei Q05547.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the fourth functional gene for protein phosphatase 1 in Drosophila melanogaster from its chromosomal location."
      Dombradi V., Mann D.J., Saunders R.D., Cohen P.T.W.
      Eur. J. Biochem. 212:177-183(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Oregon-R.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Head.
    5. "Towards a comprehensive analysis of the protein phosphatase 1 interactome in Drosophila."
      Bennett D., Lyulcheva E., Alphey L.
      J. Mol. Biol. 364:196-212(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NOP17L.

    Entry informationi

    Entry nameiPP13_DROME
    AccessioniPrimary (citable) accession number: Q05547
    Secondary accession number(s): Q9VXW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3