Reviewed, UniProtKB/Swiss-Prot Q05540 (CHIB_SOLLC)

Names and origin

Protein names

Recommended name:
    Acidic 27 kDa endochitinase
    EC=3.2.1.14

Gene names

Name:

CHI17

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space.
Induction	By fungal infection.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Pathogenesis-related protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW response to biotic stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	By similarity
	Chain	17 – 247	231	Acidic 27 kDa endochitinase		PRO_0000005300
Amino acid modifications
	Disulfide bond	206 ↔ 238		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05540-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: C1163ED1D272A5E5	FASTA	247	26,584
	10 20 30 40 50 60 MVLCCVFLLF LTGSFAQDVG TIVTSDLFNE MLKNRNDDRC PAKGFYTYDA FIAAANSFPG 70 80 90 100 110 120 FGTTGDDTAR KKEIAAFFGQ TSHETTGGSL SADGPFAGGY CFVREGNQMG SGFYGRGPIQ 130 140 150 160 170 180 LTGQSNYDLA GQAIGQDLVN NPDLVATDAT VSFKTAIWFW MTAQGNKPSC HDVITGQWTP 190 200 210 220 230 240 SAADASANRQ PGYGVITNII NGGIECGKGQ NPQVEDRIGF YRRYCTILNV APGDNLDCYD QRNFAEA

References

[1]

"Molecular characterization of four chitinase cDNAs obtained from Cladosporium fulvum-infected tomato." Danhash N., Wagemakers C.A.M., van Kan J.A.L., de Wit P.J.G.M. Plant Mol. Biol. 22:1017-1029(1993) [PubMed: 8400122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 112-115; 182-188 AND 218-221. Strain: cv. Moneymaker.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z15139 mRNA. Translation: CAA78844.1.
PIR	S37342.
UniGene	Les.3779
3D structure databases
SMR	Q05540. Positions 22-245.
ModBase	Search...
Protein family/group databases
CAZy	GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 281054.
Family and domain databases
InterPro	IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PROSITE	PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIB_SOLLC

Accession

Primary (citable) accession number: Q05540

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 19, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)