Reviewed, UniProtKB/Swiss-Prot Q05539 (CHIA_SOLLC)

Names and origin

Protein names

Recommended name:
    Acidic 26 kDa endochitinase
    EC=3.2.1.14

Gene names

Name:

CHI3

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space.
Induction	By fungal infection.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Pathogenesis-related protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to biotic stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	By similarity
	Chain	25 – 253	229	Acidic 26 kDa endochitinase		PRO_0000005299
Amino acid modifications
	Disulfide bond	212 ↔ 244		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05539-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 6C78CA3750DE6D7A	FASTA	253	27,632
	10 20 30 40 50 60 MKFNIVSPVA LSCLFFLFLT GTLAQNAGSI VTRELFEQML SFRNNDACPA KGFYTYDAFI 70 80 90 100 110 120 AAANSFPGFG TAGDDTARKK EIAAFFGQTS HETNGGSAGT FTGGYCFVKQ IEQSDRYYGR 130 140 150 160 170 180 GPIQLTHQSN YERAGQGIGV GQELVNNPDL VATDPIISFK TAIWFWMTEQ DNKPSCHNVI 190 200 210 220 230 240 IGQWTPSPKD TAANRVPGYG VITNIINGQF ECGMGPNTAA ESRIGFYRRY CGMLNVPTGE 250 NLDCNNQKNF AQG

References

[1]

"Molecular characterization of four chitinase cDNAs obtained from Cladosporium fulvum-infected tomato." Danhash N., Wagemakers C.A.M., van Kan J.A.L., de Wit P.J.G.M. Plant Mol. Biol. 22:1017-1029(1993) [PubMed: 8400122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 134-152 AND 225-228. Strain: cv. Moneymaker.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z15141 mRNA. Translation: CAA78846.1.
PIR	S37343.
UniGene	Les.122
3D structure databases
HSSP	HSSP built from PDB template 1CNS based on UniProtKB P23951.
ModBase	Search...
Protein family/group databases
CAZy	GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 281054.
Family and domain databases
InterPro	IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
ProDom	PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_SOLLC

Accession

Primary (citable) accession number: Q05539

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)