Q05534 (PHOG_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein pacG

Alternative name(s):
Nonrepressible acid phosphatase regulator pacG

Gene names

Name:	pacG
ORF Names:	AN1414

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella › Emericella nidulans › mitosporic Emericella nidulans

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transcription factor that acts as a positive regulator of nonrepressible acid phosphatase activity. Is a major regulator of responses to nitrogen and carbon starvation and is essential for the expression of genes involved in vegetative incompatibility (like pin-c, het-6, and tol). Vegetative incompatibility is a non-self-recognition system ubiquitous in filamentous fungi which results in programmed cell death By similarity.
Subcellular location	Nucleus. Cytoplasm By similarity.
Sequence similarities	Contains 1 NDT80 DNA-binding domain.
Caution	Was originally thought (Ref.3) to be a phosphatase.
Sequence caution	The sequence CBF84810.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAA64544.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Protein pacG		PRO_0000023977
Regions
	DNA binding	79 – 326	248	NDT80
Experimental info
	Sequence conflict	376	1	G → R in AAA33289. Ref.3
	Sequence conflict	423	1	R → L in AAA33289. Ref.3
	Sequence conflict	447	1	G → C in AAA33289. Ref.3
	Sequence conflict	451	1	P → S in AAA33289. Ref.3
	Sequence conflict	460 – 461	2	AS → VV in AAA33289. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q05534 [UniParc]. Last modified November 16, 2011. Version 3. Checksum: 9BC5AB22BCDC665E	FASTA	566	61,871
	10 20 30 40 50 60 MEGFDTMALP YLASPLSLGN MQGTDYLNAL QGMDLPDQRS NFDSETFVSG DDLTFAHLSP 70 80 90 100 110 120 QTLKRFSSGY EDSFPEMVTS FDPPPPAEPP ADSSIDHNNK LLSFSMPVYP WTLLDYSFRR 130 140 150 160 170 180 ASISISAQLH GMFFLAESPW TTSPTENAPP QQGAELTCYR RNLFQITGSV TLPRALRYII 190 200 210 220 230 240 TDTGDRIPIV AHELTVSATE SVEGNSVKII SVPWKTPAAN DAGKDTGNSS NTAAKVEKEP 250 260 270 280 290 300 PAIPLDMLTG QDLDADYATF PIAWKRLQFR VATANNGRRK ELQQHFVVRL RVVATLSTGM 310 320 330 340 350 360 KTPICEVHSG PVIVRGRSPR NFQSRKDLPL SGSAAASRKN AQAAAASNNL TRTSPSLTDK 370 380 390 400 410 420 AKTVVKSSSP ETSSNGVPQQ SPPNWALATN STLPPPTTTT LPHSSVYSQS SPEFSRPVEA 430 440 450 460 470 480 HRRTTSAIAA PINLSLLDDD SLNLSNGDSR PHTSFSNDLA SKSLSVDSGR PVKMRKVSHS 490 500 510 520 530 540 MPQAQSRSTS ATFLNTANFQ QMLPVPFTSE SADVLYEYFP LGLEDWQGPV DAVYRPHVVH 550 560 HTNMPQMKYI TARGQSKRYF AAEDVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[2]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"Characterization of an Aspergillus nidulans genomic DNA fragment conferring phosphate-non-repressible acid-phosphatase activity." Macrae W.D., Buxton F.P., Sibley S., Garven S., Gwynne D.I., Arst H.N. Jr., Davies R.W. Gene 130:247-251(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 132-461. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACD01000022 Genomic DNA. Translation: EAA64544.1. Sequence problems. BN001307 Genomic DNA. Translation: CBF84810.1. Sequence problems. M96993 Genomic DNA. Translation: AAA33289.1.
PIR	JN0783.
RefSeq	XP_659018.1. XM_653926.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2875501.
KEGG	ani:AN1414.2.
Phylogenomic databases
eggNOG	NOG79465.
HOGENOM	HOG000157088.
OrthoDB	EOG4JQ75S.
Family and domain databases
Gene3D	2.60.40.1390. 1 hit.
InterPro	IPR024061. NDT80_DNA-bd_dom. IPR008967. p53-like_TF_DNA-bd. [Graphical view]
Pfam	PF05224. NDT80_PhoG. 1 hit. [Graphical view]
SUPFAM	SSF49417. P53_like_DNA_bnd. 1 hit.
PROSITE	PS51517. NDT80. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHOG_EMENI

Accession

Primary (citable) accession number: Q05534
Secondary accession number(s): C8VM27, Q5BDG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 16, 2011
Last modified:	April 3, 2013
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program