Reviewed, UniProtKB/Swiss-Prot Q05534 (PHOG_EMENI)

Names and origin

Protein names

Recommended name:
    Protein pacG
Alternative name(s):
    Nonrepressible acid phosphatase regulator pacG

Gene names

Name:	pacG
ORF Names:	AN1414

Organism

Emericella nidulans (Aspergillus nidulans)

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transcription factor that acts as a positive regulator of nonrepressible acid phosphatase activity. Is a major regulator of responses to nitrogen and carbon starvation and is essential for the expression of genes involved in vegetative incompatibility (like pin-c, het-6, and tol). Vegetative incompatibility is a non-self-recognition system ubiquitous in filamentous fungi which results in programmed cell death By similarity.
Subcellular location	Nucleus. Cytoplasm By similarity.
Sequence similarities	Contains 1 NDT80 domain.
Caution	Was originally thought (Ref.2) to be a phosphatase.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Protein pacG		PRO_0000023977
Regions
	Domain	24 – 194	171	NDT80
	DNA binding	1 – 193	193	By similarity
Experimental info
	Sequence conflict	245	1	G → R in AAA33289. Ref.2
	Sequence conflict	292	1	R → L in AAA33289. Ref.2
	Sequence conflict	316	1	G → C in AAA33289. Ref.2
	Sequence conflict	320	1	P → S in AAA33289. Ref.2
	Sequence conflict	329 – 330	2	AH → VV in AAA33289. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q05534-1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: DB00F492A2771937	FASTA	351	38,056
	10 20 30 40 50 60 MFFLAESPWT TSPTENAPPQ QGAELTCYRR NLFQITGSVT LPRALRYIIT DTGDRIPIVA 70 80 90 100 110 120 HELTVSATES VEGNSVKIIS VPWKTPAAND AGKDTGNSSN TAAKVEKEPP AIPLDMLTGQ 130 140 150 160 170 180 DLDADYATFP IAWKRLQFRV ATANNGRRKE LQQHFVVRLR VVATLSTGMK TPICEVHSGP 190 200 210 220 230 240 VIVRGRSPRN FQSRKDLPLS GSAAASRKNA QAAAASNNLT RTSPSLTDKA KTVVKSSSPE 250 260 270 280 290 300 TSSNGVPQQS PPNWALATNS TLPPPTTTTL PHSSVYSQSS PEFSRPVEAH RRTTSAIAAP 310 320 330 340 350 INLSLLDDDS LNLSNGDSRP HTSFSNDLAH ILLLRPYVHL DGHTRELFHV Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.
[2]	"Characterization of an Aspergillus nidulans genomic DNA fragment conferring phosphate-non-repressible acid-phosphatase activity." Macrae W.D., Buxton F.P., Sibley S., Garven S., Gwynne D.I., Arst H.N. Jr., Davies R.W. Gene 130:247-251(1993) [PubMed: 7916713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-330. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACD01000022 Genomic DNA. Translation: EAA64544.1. M96993 Genomic DNA. Translation: AAA33289.1.
PIR	JN0783.
RefSeq	XP_659018.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2875501.
KEGG	ani:AN1414.2.
Enzyme and pathway databases
BRENDA	3.1.3.2. 3859.
Family and domain databases
InterPro	IPR007888. NDT80_DNA_bd. [Graphical view]
Pfam	PF05224. NDT80_PhoG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHOG_EMENI

Accession

Primary (citable) accession number: Q05534
Secondary accession number(s): Q5BDG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)