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Q05533 (INM2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inositol monophosphatase 2
Short name=IMP 2
Short name=IMPase 2
EC=3.1.3.25
Alternative name(s):
Inositol-1(or 4)-monophosphatase 2
Gene names
Name:INM2
Synonyms:IMP2
Ordered Locus Names:YDR287W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and involved in the inositol cycle of calcium signaling. Ref.4 Ref.5

Catalytic activity

Myo-inositol phosphate + H2O = myo-inositol + phosphate.

Cofactor

Magnesium.

Enzyme regulation

Inhibited by Li+ and Na+. Ref.4

Pathway

Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.

Sequence similarities

Belongs to the inositol monophosphatase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.12 mM for inositol 1-phosphate Ref.4

Vmax=16 µmol/min/mg enzyme for inositol 1-phosphate

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292Inositol monophosphatase 2
PRO_0000245568

Regions

Region96 – 994Substrate binding By similarity

Sites

Metal binding751Magnesium 1 By similarity
Metal binding941Magnesium 1 By similarity
Metal binding941Magnesium 2 By similarity
Metal binding961Magnesium 1; via carbonyl oxygen By similarity
Metal binding971Magnesium 2 By similarity
Metal binding2311Magnesium 2 By similarity
Binding site751Substrate By similarity
Binding site2311Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05533 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: CEA9D943F69E2082

FASTA29232,093
        10         20         30         40         50         60 
MVLTRQVLEE VENTFIELLR SKIGPLVKSH AGTNFCSYDD KANGVDLVTA LDKQIESIIK 

        70         80         90        100        110        120 
ENLTAKYPSF KFIGEETYVK GVTKITNGPT FIVDPIDGTT NFIHGYPYSC TSLGLAEMGK 

       130        140        150        160        170        180 
PVVGVVFNPH LNQLFHASKG NGAFLNDQEI KVSKRPLILQ KSLIALEGGS ERTEGSQGNF 

       190        200        210        220        230        240 
DKKMNTYKNL LSESGAFVHG FRSAGSAAMN ICYVASGMLD AYWEGGCWAW DVCAGWCILE 

       250        260        270        280        290 
EAGGIMVGGN CGEWNIPLDR RCYLAIRGGC ESMEQKRFAE SFWPHVAGEL EY

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The yeast inositol monophosphatase is a lithium- and sodium-sensitive enzyme encoded by a non-essential gene pair."
Lopez F., Leube M., Gil-Mascarell R., Navarro-Avino J.P., Serrano R.
Mol. Microbiol. 31:1255-1264(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
[5]"Yeast inositol mono- and trisphosphate levels are modulated by inositol monophosphatase activity and nutrients."
Navarro-Avino J.P., Belles J.M., Serrano R.
Biochem. Biophys. Res. Commun. 302:41-45(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U51031 Genomic DNA. Translation: AAB64472.1.
AY557746 Genomic DNA. Translation: AAS56072.1.
BK006938 Genomic DNA. Translation: DAA12127.1.
PIRS70117.
RefSeqNP_010573.3. NM_001180595.3.

3D structure databases

ProteinModelPortalQ05533.
SMRQ05533. Positions 41-265.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5626N.
MINTMINT-508245.
STRING4932.YDR287W.

Proteomic databases

PaxDbQ05533.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR287W; YDR287W; YDR287W.
GeneID851881.
KEGGsce:YDR287W.

Organism-specific databases

CYGDYDR287w.
SGDS000002695. INM2.

Phylogenomic databases

eggNOGCOG0483.
GeneTreeENSGT00390000014699.
HOGENOMHOG000282238.
KOK01092.
OMANFIHGYP.
OrthoDBEOG40312B.

Enzyme and pathway databases

BioCycYEAST:G3O-29851-MONOMER.
YEAST:MONOMER3O-11.
UniPathwayUPA00823; UER00788.

Gene expression databases

GenevestigatorQ05533.
GermOnlineYDR287W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERPTHR20854. PTHR20854. 1 hit.
PfamPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSPR00377. IMPHPHTASES.
PROSITEPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969850.

Entry information

Entry nameINM2_YEAST
AccessionPrimary (citable) accession number: Q05533
Secondary accession number(s): D6VSR7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: May 29, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents