Reviewed,
UniProtKB/Swiss-Prot Q05533 (INM2_YEAST)
Last modified
February 9, 2010.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Inositol monophosphatase 2 Short name=IMPase 2 Short name=IMP 2 EC=3.1.3.25 Alternative name(s): Inositol-1(or 4)-monophosphatase 2 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 292 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and involved in the inositol cycle of calcium signaling. Ref.3 Ref.4 |
| Catalytic activity | Myo-inositol phosphate + H2O = myo-inositol + phosphate. |
| Cofactor | Magnesium. |
| Enzyme regulation | Inhibited by Li+ and Na+. Ref.3 |
| Pathway | Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2. |
| Sequence similarities | Belongs to the inositol monophosphatase family. |
| Biophysicochemical properties | Kinetic parameters: KM=0.12 mM for inositol 1-phosphate Vmax=16 µmol/min/mg enzyme for inositol 1-phosphate |
Ontologies
| Keywords | |
|---|---|
| Ligand | Lithium Magnesium Metal-binding |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | inositol phosphate dephosphorylation Ref.3 Inferred from direct assay. Source: SGD |
| Molecular function | inositol-1(or 4)-monophosphatase activity Ref.3 Inferred from direct assay. Source: SGD lithium ion bindingInferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 292 | 292 | Inositol monophosphatase 2 | PRO_0000245568 | |||||
Regions | |||||||||
| Region | 96 – 99 | 4 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Metal binding | 75 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 94 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 94 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 96 | 1 | Magnesium 1; via carbonyl oxygen By similarity | ||||||
| Metal binding | 97 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 231 | 1 | Magnesium 2 By similarity | ||||||
| Binding site | 75 | 1 | Substrate By similarity | ||||||
| Binding site | 231 | 1 | Substrate By similarity | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U51031 Genomic DNA. Translation: AAB64472.1. AY557746 Genomic DNA. Translation: AAS56072.1. |
| PIR | S70117. |
| RefSeq | NP_010573.1. |
3D structure databases | |
| SMR | Q05533. Positions 22-259. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-5626N. |
| IntAct | Q05533. 1 interaction. |
| STRING | Q05533. |
Genome annotation databases | |
| Ensembl | YDR287W; YDR287W; YDR287W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 851881. |
| KEGG | sce:YDR287W. |
| NMPDR | fig|4932.3.peg.1335. |
Organism-specific databases | |
| CYGD | YDR287w. |
| SGD | S000002695. INM2. |
Phylogenomic databases | |
| eggNOG | fuNOG06264. |
| HOGENOM | HBG730251. |
| OMA | LDAYWEG. |
| OrthoDB | EOG9DJMCX. |
| PhylomeDB | Q05533. |
Enzyme and pathway databases | |
| BRENDA | 3.1.3.25. 250. |
Gene expression databases | |
| ArrayExpress | Q05533. |
| Genevestigator | Q05533. |
| GermOnline | YDR287W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view] |
| PANTHER | PTHR20854. Inositol_P. 1 hit. |
| Pfam | PF00459. Inositol_P. 1 hit. [Graphical view] |
| PRINTS | PR00377. IMPHPHTASES. |
| PROSITE | PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 969850. |
Entry information
| Entry name | INM2_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q05533 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


