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Protein

4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase

Gene

kduI

Organism
Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of 5-dehydro-4-deoxy-D-glucuronate to 3-deoxy-D-glycero-2,5-hexodiulosonate.1 Publication

Catalytic activityi

5-dehydro-4-deoxy-D-glucuronate = 3-deoxy-D-glycero-2,5-hexodiulosonate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: pectin degradation

This protein is involved in step 4 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase A (pemA), Pectinesterase B (pemB)
  2. Pectate lyase L (pelL), Pectate lyase E (pelE), Pectate lyase A (pelA)
  3. Oligogalacturonate lyase (ogl)
  4. 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI)
  5. 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (kduD)
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi196ZincBy similarity1
Metal bindingi198ZincBy similarity1
Metal bindingi203ZincBy similarity1
Metal bindingi245ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciDDAD198628:G1GMV-2374-MONOMER.
MetaCyc:MONOMER-17020.
UniPathwayiUPA00545; UER00826.

Names & Taxonomyi

Protein namesi
Recommended name:
4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC:5.3.1.17)
Alternative name(s):
5-keto-4-deoxyuronate isomerase
DKI isomerase
Gene namesi
Name:kduI
Ordered Locus Names:Dda3937_03727
OrganismiDickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))
Taxonomic identifieri198628 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaeDickeya
Proteomesi
  • UP000006859 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are unable to grow on polygalacturonate (PGA) or digalacturonate, but can grow on galacturonate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002154911 – 2784-deoxy-L-threo-5-hexosulose-uronate ketol-isomeraseAdd BLAST278

Expressioni

Inductioni

Induced by galacturonate and at a higher level by polygalacturonate. Is expressed under the control of KdgR, but is not controlled by PecS. Is repressed by glucose.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi198628.Dda3937_03727.

Structurei

3D structure databases

SMRiQ05529.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KduI family.Curated

Phylogenomic databases

eggNOGiENOG4105D50. Bacteria.
COG3717. LUCA.
HOGENOMiHOG000124379.
KOiK01815.
OMAiCQLQMGM.
OrthoDBiPOG091H066L.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
2.60.120.520. 1 hit.
HAMAPiMF_00687. KduI. 1 hit.
InterProiView protein in InterPro
IPR007045. KduI.
IPR021120. KduI/IolB_isomerase.
IPR027449. KduI_N.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin_sf.
PANTHERiPTHR38461. PTHR38461. 1 hit.
PfamiView protein in Pfam
PF04962. KduI. 1 hit.
PIRSFiPIRSF006625. KduI. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVRQSIHSD HARQLDTAGL RREFLIEHIF DADACTMTYS HIDRIIVGGV
60 70 80 90 100
MPVHQAVTVG EDVGKQLGVS YFLERRELGA INIGGAGVVS VDGERYAIGH
110 120 130 140 150
EEAIYIGKGA RDIRFTSVDP AKPARFYYNS APAHTTFPTR KITAAEASPQ
160 170 180 190 200
TIGDDATSNR RTINKYIVPD VLPTCQLTMG LTKLAEGNLW NTMPCHTHER
210 220 230 240 250
RMEVYFYFDM DEETAVFHMM GQPQETRHIL VHNEQAVISP SWSIHSGVGT
260 270
KRYTFIWGMV GENQVFSDMD HVKVSELR
Length:278
Mass (Da):31,240
Last modified:November 30, 2010 - v2
Checksum:i9D052B0C9B8B4A5C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63V → D in CAA43988 (PubMed:1766386).Curated1
Sequence conflicti70S → T in CAA43988 (PubMed:1766386).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62073 Genomic DNA. Translation: CAA43988.1.
CP002038 Genomic DNA. Translation: ADM98615.1.
PIRiS17710.
RefSeqiWP_013318065.1. NC_014500.1.

Genome annotation databases

EnsemblBacteriaiADM98615; ADM98615; Dda3937_03727.
GeneIDi9733850.
KEGGiddd:Dda3937_03727.
PATRICifig|198628.6.peg.2392.

Similar proteinsi

Entry informationi

Entry nameiKDUI_DICD3
AccessioniPrimary (citable) accession number: Q05529
Secondary accession number(s): E0SDC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 30, 2010
Last modified: February 28, 2018
This is version 80 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome