Q05528 (KDUD_DICD3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
EC=1.1.1.127

Alternative name(s):
2-keto-3-deoxygluconate 5-dehydrogenase
2-keto-3-deoxygluconate oxidoreductase
Short name=KDG oxidoreductase

Gene names

Name:	kduD
Ordered Locus Names:	Dda3937_04595

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) [Complete proteome] [HAMAP]

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reduction of 2,5-diketo-3-deoxygluconate (DKII or 4,6-dihydroxy-2,5-dioxohexanoate) into 2-keto-3-deoxygluconate (KDG or 2-dehydro-3-deoxygluconate) with a concomitant oxidation of NADH. Ref.1
Catalytic activity	2-dehydro-3-deoxy-D-gluconate + NAD+ = (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH. Ref.1
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 5/5.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase activity Inferred from electronic annotation. Source: EC 2-deoxy-D-gluconate 3-dehydrogenase activity Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase		PRO_0000054716
Regions
	Nucleotide binding	14 – 38	25	NAD By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	145	1	Substrate By similarity
Experimental info
	Sequence conflict	154	1	R → P in CAA43989. Ref.1
	Sequence conflict	163 – 164	2	SA → KR in CAA43989. Ref.1
	Sequence conflict	171 – 172	2	LL → IV in CAA43989. Ref.1
	Sequence conflict	198 – 199	2	QL → HV in CAA43989. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q05528 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 53D15DE7E1FAD8DA	FASTA	253	27,227
	10 20 30 40 50 60 MILNTFNLQG KVALITGCDT GLGQGMAVGL AEAGCDIVGV NIVEPKETIE KVTAVGRRFL 70 80 90 100 110 120 SLTADMSDIS GHAALVEKAV AEFGKVDILV NNAGIIRRED AIEFSEKNWD DVMNLNIKSV 130 140 150 160 170 180 FFMSQTVARQ FIKQGHGGKI INIASMLSFQ GGIRVPSYTA SKSAVMGITR LLANEWAKHN 190 200 210 220 230 240 INVNAIAPGY MATNNTQQLR ADQDRSKEIL DRIPAGRWGL PQDLQGPAVF LASSASDYVN 250 GYTIAVDGGW LAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of an Erwinia chrysanthemi gene cluster involved in pectin degradation." Condemine G., Robert-Baudouy J. Mol. Microbiol. 5:2191-2202(1991) [PubMed: 1766386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, FUNCTION IN PECTIN DEGRADATION. Strain: 3937.
[2]	"Complete genome sequence of Dickeya dadantii 3937." International Erwinia Consortium Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3937.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62073 Genomic DNA. Translation: CAA43989.1. CP002038 Genomic DNA. Translation: ADM98616.1.
PIR	S17711.
RefSeq	YP_003883173.1. NC_014500.1.
3D structure databases
ProteinModelPortal	Q05528.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9733851.
GenomeReviews	Gene locus Dda3937_04595 in contig CP002038_GR.
KEGG	ddd:Dda3937_04595.
PATRIC	42317142. VBIDicDad25310_2393.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15643.
Family and domain databases
InterPro	IPR011286. 2-deoxy-D-gluc_3_DH. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00065.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01832. KduD. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDUD_DICD3

Accession

Primary (citable) accession number: Q05528
Secondary accession number(s): E0SDC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 30, 2010
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program