Reviewed, UniProtKB/Swiss-Prot Q05526 (PELW_DICD3)

Names and origin

Protein names

Recommended name:
    Pectate disaccharide-lyase
    EC=4.2.2.9
Alternative name(s):
    Exopolygalacturonate lyase
      Short name=ExoPL

Gene names

Name:	pelW
Synonyms:	kdgC

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of unsaturated digalacturonates from polygalacturonate or short oligogalacturonates.
Catalytic activity	Eliminative cleavage of 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate from the reducing end of pectate, i.e. de-esterified pectin.
Cofactor	Copper. Manganese. Nickel.
Pathway	Glycan metabolism; pectin degradation.
Subcellular location	Cytoplasm.
Induction	By galacturonate and PGA, and inhibited by EDTA.
Sequence similarities	Belongs to the polysaccharide lyase 2 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Copper Manganese Nickel
Molecular function	Lyase
Gene Ontology (GO)
Biological process	pectin catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate disaccharide-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Pectate disaccharide-lyase		PRO_0000212998

Sequences

	Sequence		Length	Mass (Da)
	Q05526-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: CC1FF8782E33F1E8	FASTA	553	64,074
	10 20 30 40 50 60 MSIFTDLNTS RKWQIDQWLS AVNSHIEKIQ QYGHSVVNPT PLLADGFEIK TQSPVVWQFP 70 80 90 100 110 120 DGHDAPISNF ASQQNWLRLL ISMSVITETE KYRHLAFCQS EYFLNRFVDE NSGLFYWGGH 130 140 150 160 170 180 RFINLDTLAS EGPESKSMVH ELKHHLPYYE FLHQVNPEKT RHFIQGFWNA HVEDWSCLDL 190 200 210 220 230 240 GRHGDYARQR DPDVFLHSRH DVVTPANWPE LPLTKGLTFV NAGTDLIYAA FVYARHTGDA 250 260 270 280 290 300 HAAAWGKHLY RQYVLARNPE TGMPVYQFSS PLQRQPVPAD DNQTQSWFGD RAQRQFGPEF 310 320 330 340 350 360 GAIAREANVL FRDMRPLLID NPLAMLDILR HQPDAEILTW VIAGLKNYYQ YAYDVNSNSL 370 380 390 400 410 420 RPMWNNGQDM TDYCFKRDGY YGKAGTVLKP FPLEGDYLLP LVRAWLLSDD DDLHTLIVTM 430 440 450 460 470 480 LSRLEKQGIH QSASPFLLLA ITELAHAKQS AQWAEYAWQM AEILFKRYFH HGLFVRSEHH 490 500 510 520 530 540 RYVRLDDPFP AILLTLIAAC RNKWSEVPAV LTQGGYIHGD YRINGESRVI YDTGIYLPRK 550 INPLILFLQI HHY

References

[1]	"Analysis of an Erwinia chrysanthemi gene cluster involved in pectin degradation." Condemine G., Robert-Baudouy J. Mol. Microbiol. 5:2191-2202(1991) [PubMed: 1766386] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937." Shevchik V.E., Condemine G., Robert-Baudouy J., Hugouvieux-Cotte-Pattat N. J. Bacteriol. 181:3912-3919(1999) [PubMed: 10383957] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION TO C-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62073 Genomic DNA. Translation: CAA43990.1.
PIR	S17712.
3D structure databases
SMR	Q05526. Positions 17-537.
ModBase	Search...
Protein family/group databases
CAZy	PL2. Polysaccharide Lyase Family 2.
Family and domain databases
InterPro	IPR010702. Pectate_lyase_2. [Graphical view]
Pfam	PF06917. Pectate_lyase_2. 1 hit. [Graphical view]
PIRSF	PIRSF001432. Pect_lyase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PELW_DICD3

Accession

Primary (citable) accession number: Q05526

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	May 30, 2000
Last modified:	January 19, 2010
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)