Q05519 (SRS11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/arginine-rich splicing factor 11

Alternative name(s):
Arginine-rich 54 kDa nuclear protein
Short name=p54
Splicing factor, arginine/serine-rich 11

Gene names

Name:	SRSF11
Synonyms:	SFRS11

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function in pre-mRNA splicing.
Subunit structure	Interacts with PUF60. Ref.5
Subcellular location	Nucleus. Note: Colocalizes with spliceosome components.
Sequence similarities	Belongs to the splicing factor SR family. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA 3'-end processing Traceable author statement. Source: Reactome mRNA export from nucleus Traceable author statement. Source: Reactome mRNA splicing, via spliceosome Traceable author statement. Source: Reactome termination of RNA polymerase II transcription Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ETS1	P14921	2	EBI-1051785,EBI-913209

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q05519-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q05519-2)

The sequence of this isoform differs from the canonical sequence as follows:
     420-420: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Serine/arginine-rich splicing factor 11		PRO_0000081939
Regions
	Domain	33 – 113	81	RRM
	Repeat	247 – 255	9	1
	Repeat	258 – 265	8	2
	Repeat	267 – 274	8	3
	Repeat	275 – 282	8	4
	Repeat	285 – 292	8	5
	Repeat	293 – 300	8	6
	Repeat	302 – 309	8	7
	Repeat	321 – 328	8	8
	Repeat	334 – 341	8	9
	Repeat	346 – 353	8	10
	Region	247 – 353	107	10 X 8 AA approximate repeats of R-R-S-R-S-R-S-R
	Compositional bias	17 – 31	15	Poly-Gly
	Compositional bias	252 – 259	8	Poly-Arg
	Compositional bias	291 – 294	4	Poly-Arg
	Compositional bias	341 – 349	9	Poly-Arg
	Compositional bias	397 – 404	8	Poly-Lys
Amino acid modifications
	Modified residue	207	1	Phosphoserine Ref.8 Ref.9 Ref.10 Ref.12
	Modified residue	414	1	Phosphoserine Ref.10
	Modified residue	434	1	Phosphoserine Ref.8 Ref.10 Ref.12
	Modified residue	447	1	Phosphothreonine Ref.10 Ref.12
	Modified residue	449	1	Phosphoserine Ref.10 Ref.12
	Modified residue	464	1	Phosphoserine Ref.10
	Modified residue	483	1	Phosphoserine Ref.6 Ref.9 Ref.10 Ref.12
Natural variations
	Alternative sequence	420	1	Missing in isoform 2.		VSP_043376

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1211E96DDC0A3182	FASTA	484	53,542
	10 20 30 40 50 60 MSNTTVVPST AGPGPSGGPG GGGGGGGGGG GTEVIQVTNV SPSASSEQMR TLFGFLGKID 70 80 90 100 110 120 ELRLFPPDDS PLPVSSRVCF VKFHDPDSAV VAQHLTNTVF VDRALIVVPY AEGVIPDEAK 130 140 150 160 170 180 ALSLLAPANA VAGLLPGGGL LPTPNPLTQI GAVPLAALGA PTLDPALAAL GLPGANLNSQ 190 200 210 220 230 240 SLAADQLLKL MSTVDPKLNH VAAGLVSPSL KSDTSSKEIE EAMKRVREAQ SLISAAIEPD 250 260 270 280 290 300 KKEEKRRHSR SRSRSRRRRT PSSSRHRRSR SRSRRRSHSK SRSRRRSKSP RRRRSHSRER 310 320 330 340 350 360 GRRSRSTSKT RDKKKEDKEK KRSKTPPKSY STARRSRSAS RERRRRRSRS GTRSPKKPRS 370 380 390 400 410 420 PKRKLSRSPS PRRHKKEKKK DKDKERSRDE RERSTSKKKK SKDKEKDRER KSESDKDVKQ 430 440 450 460 470 480 VTRDYDEEEQ GYDSEKEKKE EKKPIETGSP KTKECSVEKG TGDSLRESKV NGDDHHEEDM DMSD
	Isoform 2 [UniParc]. Checksum: 5A40AFD8B7599976	FASTA	483	53,414
	10 20 30 40 50 60 MSNTTVVPST AGPGPSGGPG GGGGGGGGGG GTEVIQVTNV SPSASSEQMR TLFGFLGKID 70 80 90 100 110 120 ELRLFPPDDS PLPVSSRVCF VKFHDPDSAV VAQHLTNTVF VDRALIVVPY AEGVIPDEAK 130 140 150 160 170 180 ALSLLAPANA VAGLLPGGGL LPTPNPLTQI GAVPLAALGA PTLDPALAAL GLPGANLNSQ 190 200 210 220 230 240 SLAADQLLKL MSTVDPKLNH VAAGLVSPSL KSDTSSKEIE EAMKRVREAQ SLISAAIEPD 250 260 270 280 290 300 KKEEKRRHSR SRSRSRRRRT PSSSRHRRSR SRSRRRSHSK SRSRRRSKSP RRRRSHSRER 310 320 330 340 350 360 GRRSRSTSKT RDKKKEDKEK KRSKTPPKSY STARRSRSAS RERRRRRSRS GTRSPKKPRS 370 380 390 400 410 420 PKRKLSRSPS PRRHKKEKKK DKDKERSRDE RERSTSKKKK SKDKEKDRER KSESDKDVKV 430 440 450 460 470 480 TRDYDEEEQG YDSEKEKKEE KKPIETGSPK TKECSVEKGT GDSLRESKVN GDDHHEEDMD MSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of a human arginine-rich nuclear protein that colocalizes with spliceosome components." Chaudhary N., McMahon C., Blobel G. Proc. Natl. Acad. Sci. U.S.A. 88:8189-8193(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hepatoma.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.
[5]	"PUF60: a novel U2AF65-related splicing activity." Page-McCaw P.S., Amonlirdviman K., Sharp P.A. RNA 5:1548-1560(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PUF60.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-483, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207 AND SER-434, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207 AND SER-483, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207; SER-414; SER-434; THR-447; SER-449; SER-464 AND SER-483, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207; SER-434; THR-447; SER-449 AND SER-483, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74002 mRNA. Translation: AAA35554.1. AL353771 Genomic DNA. Translation: CAI22334.1. AL353771 Genomic DNA. Translation: CAI22335.1. CH471059 Genomic DNA. Translation: EAX06459.1. BC040436 mRNA. Translation: AAH40436.1.
IPI	IPI00464952. IPI00641287.
PIR	A40988.
RefSeq	NP_001177916.1. NM_001190987.1. NP_004759.1. NM_004768.3.
UniGene	Hs.479693.
3D structure databases
ProteinModelPortal	Q05519.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q05519. 8 interactions.
MINT	MINT-4655237.
STRING	9606.ENSP00000359988.
PTM databases
PhosphoSite	Q05519.
Polymorphism databases
DMDM	8134672.
Proteomic databases
PaxDb	Q05519.
PRIDE	Q05519.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370950; ENSP00000359988; ENSG00000116754. ENST00000370951; ENSP00000359989; ENSG00000116754. ENST00000405432; ENSP00000384357; ENSG00000116754.
GeneID	9295.
KEGG	hsa:9295.
UCSC	uc001des.3. human. uc001det.3. human.
Organism-specific databases
CTD	9295.
GeneCards	GC01P070672.
HGNC	HGNC:10782. SRSF11.
HPA	HPA008762.
MIM	602010. gene.
neXtProt	NX_Q05519.
PharmGKB	PA35698.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG277302.
HOGENOM	HOG000121772.
HOVERGEN	HBG058422.
InParanoid	Q05519.
KO	K12899.
OrthoDB	EOG4J9N10.
PhylomeDB	Q05519.
Enzyme and pathway databases
Reactome	REACT_1675. mRNA Processing. REACT_1788. Transcription. REACT_71. Gene Expression. REACT_78. Post-Elongation Processing of the Transcript.
Gene expression databases
ArrayExpress	Q05519.
Bgee	Q05519.
CleanEx	HS_SFRS11.
Genevestigator	Q05519.
GermOnline	ENSG00000116754. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SRSF11. human.
GenomeRNAi	9295.
NextBio	34827.
SOURCE	Search...

Entry information

Entry name

SRS11_HUMAN

Accession

Primary (citable) accession number: Q05519
Secondary accession number(s): Q5T758, Q8IWE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.