ID   MAAL_CLOTT              Reviewed;         413 AA.
AC   Q05514;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   20-JAN-2009, entry version 55.
DE   RecName: Full=Methylaspartate ammonia-lyase;
DE            EC=4.3.1.2;
DE   AltName: Full=Beta-methylaspartase;
OS   Clostridium tetanomorphum.
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1553;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-26.
RC   STRAIN=ATCC 15920 / DSM 528 / H1;
RX   MEDLINE=93041773; PubMed=1420191; DOI=10.1021/bi00159a015;
RA   Goda S.K., Minton N.P., Botting N.P., Gani D.;
RT   "Cloning, sequencing, and expression in Escherichia coli of the
RT   Clostridium tetanomorphum gene encoding beta-methylaspartase and
RT   characterization of the recombinant protein.";
RL   Biochemistry 31:10747-10756(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24, AND PROTEIN SEQUENCE OF
RP   1-24.
RC   STRAIN=ATCC 15920 / DSM 528 / H1;
RX   MEDLINE=93202282; PubMed=8454064; DOI=10.1016/0014-5793(93)80042-S;
RA   Brecht M., Kellermann J., Plueckthun A.;
RT   "Cloning and sequencing of glutamate mutase component E from
RT   Clostridium tetanomorphum.";
RL   FEBS Lett. 319:84-89(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-71.
RC   STRAIN=NCIMB 11547;
RX   MEDLINE=93154518; PubMed=8428631; DOI=10.1016/0014-5793(93)81488-L;
RA   Holloway D.E., Marsh E.N.G.;
RT   "Cloning and sequencing of glutamate mutase component E from
RT   Clostridium tetanomorphum. Organization of the mut genes.";
RL   FEBS Lett. 317:44-48(1993).
RN   [4]
RP   PROTEIN SEQUENCE OF 1-15.
RC   STRAIN=NCIMB 11547;
RX   MEDLINE=93011908; PubMed=1397267; DOI=10.1016/0014-5793(92)81321-C;
RA   Marsh E.N.G., Holloway D.E.;
RT   "Cloning and sequencing of glutamate mutase component S from
RT   Clostridium tetanomorphum. Homologies with other cobalamin-dependent
RT   enzymes.";
RL   FEBS Lett. 310:167-170(1992).
CC   -!- CATALYTIC ACTIVITY: L-threo-3-methylaspartate = mesaconate +
CC       NH(3).
CC   -!- COFACTOR: Cobalamin.
CC   -!- PATHWAY: Amino-acid degradation; L-glutamate degradation via
CC       mesaconate pathway; acetate and pyruvate from L-glutamate: step
CC       2/4.
CC   -!- SUBUNIT: Homodimer.
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DR   EMBL; S48141; AAB24070.1; -; Genomic_DNA.
DR   EMBL; X70499; CAA49911.1; -; Genomic_DNA.
DR   EMBL; X70695; CAA50027.1; -; Genomic_DNA.
DR   PIR; B44285; B44285.
DR   PDB; 1KCZ; X-ray; 1.90 A; A/B=1-413.
DR   PDB; 1KD0; X-ray; 1.90 A; A/B=1-413.
DR   PDBsum; 1KCZ; -.
DR   PDBsum; 1KD0; -.
DR   BioCyc; MetaCyc:MON-1103; -.
DR   BRENDA; 4.3.1.2; 2080.
DR   GO; GO:0031419; F:cobalamin binding; IEA:UniProtKB-KW.
DR   GO; GO:0050897; F:cobalt ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0050096; F:methylaspartate ammonia-lyase activity; IEA:EC.
DR   InterPro; IPR006395; Met_Asp_am_lyase.
DR   Pfam; PF07476; MAAL_C; 1.
DR   Pfam; PF05034; MAAL_N; 1.
DR   PIRSF; PIRSF017107; MAL; 1.
DR   TIGRFAMs; TIGR01502; B_methylAsp_ase; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cobalamin; Cobalt; Direct protein sequencing; Lyase.
FT   CHAIN         1    413       Methylaspartate ammonia-lyase.
FT                                /FTId=PRO_0000084547.
FT   STRAND        2     11
FT   STRAND       14     18
FT   HELIX        20     24
FT   STRAND       28     30
FT   STRAND       33     36
FT   STRAND       44     49
FT   STRAND       52     59
FT   STRAND       64     69
FT   TURN         73     76
FT   HELIX        86     96
FT   HELIX        98    101
FT   HELIX       109    118
FT   HELIX       128    146
FT   HELIX       150    158
FT   HELIX       179    186
FT   STRAND      190    194
FT   HELIX       200    204
FT   HELIX       209    225
FT   STRAND      234    238
FT   HELIX       242    246
FT   TURN        247    249
FT   HELIX       251    265
FT   STRAND      270    273
FT   HELIX       281    298
FT   STRAND      302    306
FT   HELIX       313    321
FT   STRAND      325    330
FT   HELIX       333    335
FT   HELIX       339    350
FT   STRAND      354    357
FT   HELIX       365    378
FT   STRAND      381    384
FT   STRAND      387    391
FT   HELIX       392    410
SQ   SEQUENCE   413 AA;  45534 MW;  4451923DB035EF13 CRC64;
     MKIVDVLCTP GLTGFYFDDQ RAIKKGAGHD GFTYTGSTVT EGFTQVRQKG ESISVLLVLE
     DGQVAHGDCA AVQYSGAGGR DPLFLAKDFI PVIEKEIAPK LIGREITNFK PMAEEFDKMT
     VNGNRLHTAI RYGITQAILD AVAKTRKVTM AEVIRDEYNP GAEINAVPVF AQSGDDRYDN
     VDKMIIKEAD VLPHALINNV EEKLGLKGEK LLEYVKWLRD RIIKLRVRED YAPIFHIDVY
     GTIGAAFDVD IKAMADYIQT LAEAAKPFHL RIEGPMDVED RQKQMEAMRD LRAELDGRGV
     DAELVADEWC NTVEDVKFFT DNKAGHMVQI KTPDLGGVNN IADAIMYCKA NGMGAYCGGT
     CNETNRSAEV TTNIGMACGA RQVLAKPGMG VDEGMMIVKN EMNRVLALVG RRK
//