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Protein

Serine protease hepsin

Gene

Hpn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine protease that cleaves extracellular substrates, and contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL. Plays a role in cell growth and maintenance of cell morphology. Plays a role in the proteolytic processing of ACE2. Mediates the proteolytic cleavage of urinary UMOD that is required for UMOD polymerization.By similarity

Catalytic activityi

Cleavage after basic amino-acid residues, with Arg strongly preferred to Lys.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei202 – 2021Charge relay systemBy similarity
Active sitei256 – 2561Charge relay systemBy similarity
Active sitei352 – 3521Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.224.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease hepsin (EC:3.4.21.106By similarity)
Cleaved into the following 2 chains:
Gene namesi
Name:Hpn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61982. Hpn.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type II membrane protein By similarity
  • Apical cell membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence analysisAdd
BLAST
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini40 – 416377ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Serine protease hepsin non-catalytic chainSequence analysisPRO_0000027845Add
BLAST
Chaini162 – 416255Serine protease hepsin catalytic chainSequence analysisPRO_0000027846Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi76 ↔ 139PROSITE-ProRule annotation
Disulfide bondi89 ↔ 149PROSITE-ProRule annotation
Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence analysis
Disulfide bondi118 ↔ 137PROSITE-ProRule annotation
Disulfide bondi152 ↔ 276Interchain (between non-catalytic and catalytic chains)PROSITE-ProRule annotation
Disulfide bondi187 ↔ 203PROSITE-ProRule annotation
Disulfide bondi290 ↔ 358PROSITE-ProRule annotation
Disulfide bondi321 ↔ 337PROSITE-ProRule annotation
Disulfide bondi348 ↔ 380PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ05511.
PRIDEiQ05511.

Expressioni

Tissue specificityi

Widely expressed. Present in brain, heart, kidney, liver, stomach, muscle, lung, testis, skin and eye. Not expressed in ovary and thynus. In inner ear tissues, expressed in stria vascularis, modiolus, organ of Corti and spiral ganglion.1 Publication

Developmental stagei

Expressed during development in embryonic stages E8.5, E9.5, E12.5 and E19.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028644.

Structurei

3D structure databases

ProteinModelPortaliQ05511.
SMRiQ05511. Positions 48-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 15098SRCRAdd
BLAST
Domaini162 – 404243Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SRCR domain.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251822.
HOVERGENiHBG013304.
InParanoidiQ05511.
KOiK08665.
PhylomeDBiQ05511.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 1 hit.
InterProiIPR015352. Hepsin-SRCR_dom.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF09272. Hepsin-SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
ProDomiPD021735. Hepsin-SRCR. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEGGRTAP CCSRPKVAAL TVGTLLFLTG IGAASWAIVT ILLRSDQEPL
60 70 80 90 100
YQVQLSPGDS RLLVLDKTEG TWRLLCSSRS NARVAGLGCE EMGFLRALAH
110 120 130 140 150
SELDVRTAGA NGTSGFFCVD EGGLPLAQRL LDVISVCDCP RGRFLTATCQ
160 170 180 190 200
DCGRRKLPVD RIVGGQDSSL GRWPWQVSLR YDGTHLCGGS LLSGDWVLTA
210 220 230 240 250
AHCFPERNRV LSRWRVFAGA VARTSPHAVQ LGVQAVIYHG GYLPFRDPTI
260 270 280 290 300
DENSNDIALV HLSSSLPLTE YIQPVCLPAA GQALVDGKVC TVTGWGNTQF
310 320 330 340 350
YGQQAVVLQE ARVPIISNEV CNSPDFYGNQ IKPKMFCAGY PEGGIDACQG
360 370 380 390 400
DSGGHFVCED RISGTSRWRL CGIVSWGTGC ALARKPGVYT KVIDFREWIF
410
QAIKTHSEAT GMVTQP
Length:416
Mass (Da):44,926
Last modified:February 1, 1994 - v1
Checksum:iE5A9F8FA9550E180
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70900 mRNA. Translation: CAA50256.1.
PIRiS33777.
RefSeqiNP_058808.1. NM_017112.1.
UniGeneiRn.11139.

Genome annotation databases

GeneIDi29135.
KEGGirno:29135.
UCSCiRGD:61982. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70900 mRNA. Translation: CAA50256.1.
PIRiS33777.
RefSeqiNP_058808.1. NM_017112.1.
UniGeneiRn.11139.

3D structure databases

ProteinModelPortaliQ05511.
SMRiQ05511. Positions 48-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028644.

Protein family/group databases

MEROPSiS01.224.

Proteomic databases

PaxDbiQ05511.
PRIDEiQ05511.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29135.
KEGGirno:29135.
UCSCiRGD:61982. rat.

Organism-specific databases

CTDi3249.
RGDi61982. Hpn.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251822.
HOVERGENiHBG013304.
InParanoidiQ05511.
KOiK08665.
PhylomeDBiQ05511.

Miscellaneous databases

PROiQ05511.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 1 hit.
InterProiIPR015352. Hepsin-SRCR_dom.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF09272. Hepsin-SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
ProDomiPD021735. Hepsin-SRCR. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEPS_RAT
AccessioniPrimary (citable) accession number: Q05511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.