Arginyl-tRNA synthetase, cytoplasmic - Saccharomyces cerevisiae (Baker's yeast)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Ordered Locus Names:	YDR341C
ORF Names:	D9651.10

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 20600 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Hide | Top

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from direct assay. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 607

607

Arginyl-tRNA synthetase, cytoplasmic

PRO_0000151664

Regions

Motif

151 – 162

12

"HIGH" region

Amino acid modifications

Modified residue

15

1

Phosphoserine Ref.3 Ref.4 Ref.5

Secondary structure

1

.

607

Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q05506-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8349ABC0E30E50F1
Show »

FASTA

607

69,525

        10         20         30         40         50         60 
MASTANMISQ LKKLSIAEPA VAKDSHPDVN IVDLMRNYIS QELSKISGVD SSLIFPALEW 

        70         80         90        100        110        120 
TNTMERGDLL IPIPRLRIKG ANPKDLAVQW AEKFPCGDFL EKVEANGPFI QFFFNPQFLA 

       130        140        150        160        170        180 
KLVIPDILTR KEDYGSCKLV ENKKVIIEFS SPNIAKPFHA GHLRSTIIGG FLANLYEKLG 

       190        200        210        220        230        240 
WEVIRMNYLG DWGKQFGLLA VGFERYGNEE ALVKDPIHHL FDVYVRINKD IEEEGDSIPL 

       250        260        270        280        290        300 
EQSTNGKARE YFKRMEDGDE EALKIWKRFR EFSIEKYIDT YARLNIKYDV YSGESQVSKE 

       310        320        330        340        350        360 
SMLKAIDLFK EKGLTHEDKG AVLIDLTKFN KKLGKAIVQK SDGTTLYLTR DVGAAMDRYE 

       370        380        390        400        410        420 
KYHFDKMIYV IASQQDLHAA QFFEILKQMG FEWAKDLQHV NFGMVQGMST RKGTVVFLDN 

       430        440        450        460        470        480 
ILEETKEKMH EVMKKNENKY AQIEHPEEVA DLVGISAVMI QDMQGKRINN YEFKWERMLS 

       490        500        510        520        530        540 
FEGDTGPYLQ YAHSRLRSVE RNASGITQEK WINADFSLLK EPAAKLLIRL LGQYPDVLRN 

       550        560        570        580        590        600 
AIKTHEPTTV VTYLFKLTHQ VSSCYDVLWV AGQTEELATA RLALYGAARQ VLYNGMRLLG 


LTPVERM

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[3]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY.
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY.
[6]	"L-arginine recognition by yeast arginyl-tRNA synthetase." Cavarelli J., Delagoutte B., Eriani G., Gangloff J., Moras D. EMBO J. 17:5438-5448(1998) [PubMed: 9736621] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS).
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

U51032 Genomic DNA. Translation: AAB64777.1.

PIR

S70106.

RefSeq

NP_010628.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BS2	X-ray	2.75	A	1-607	[»]
1F7U	X-ray	2.20	A	1-607	[»]
1F7V	X-ray	2.90	A	1-607	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:5046N.

IntAct

Q05506. 7 interactions.

Proteomic databases

PeptideAtlas

Q05506.

PRIDE

Q05506.

Genome annotation databases

Ensembl

YDR341C. Saccharomyces cerevisiae. [Contig view]

GeneID

851942.

GenomeReviews

Gene locus YDR341C in contig Z71256_GR.

KEGG

sce:YDR341C.

NMPDR

fig|4932.3.peg.1394.

Organism-specific databases

CYGD

YDR341c.

SGD

S000002749. YDR341C.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

Q05506.

OMA

Q05506. TKEKMHE.

Enzyme and pathway databases

BRENDA

6.1.1.19. 250.

Gene expression databases

ArrayExpress

Q05506.

GermOnline

YDR341C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-synth_Ic.
IPR015945. Arg-tRNA-synth_Ic_core.
IPR005148. Arg-tRNA-synth_Ic_N.
IPR008909. DALR_anticod_bd.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

Gene3D

G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.

PANTHER

PTHR11956. Arg_tRNA-synt_1c. 1 hit.

Pfam

PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]

PRINTS

PR01038. TRNASYNTHARG.

TIGRFAMs

TIGR00456. argS. 1 hit.

PROSITE

PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

970021.

Hide | Top

Entry information

Entry name

SYRC_YEAST

Accession

Primary (citable) accession number: Q05506

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Hide | Top