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Q054T5 (SYL_LEPBL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LBL_0571
OrganismLeptospira borgpetersenii serovar Hardjo-bovis (strain L550) [Complete proteome] [HAMAP]
Taxonomic identifier355276 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira

Protein attributes

Sequence length864 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 864864Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009365

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif636 – 6405"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6391ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q054T5 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 15A6F0DFD8404D54

FASTA86499,488
        10         20         30         40         50         60 
MQYPFQEVES FWQKFWEENK SFQTNIRSSK PKFYCLDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRFKRMKGF EVLHPMGWDA FGLPAERYAM QTGIHPAITT KNNIDNFRRQ IQMIGLSYDW 

       130        140        150        160        170        180 
SRELSTTDPD YYKFTQWIFI QLYQSWFNPE LKKAESIETL IQRFSNKGSA DLDYKPFSAQ 

       190        200        210        220        230        240 
EWKQFSLVEK EKILSDFRLV YQAEIPVNWC EALGTVLANE EVEEWIDKGY EVVRKPMRQY 

       250        260        270        280        290        300 
MMRITAYADR LLEDLKLVQW PSSTLEMQKN WIGKSEGLEI TFPFKKPLKS GSDGIRIFTT 

       310        320        330        340        350        360 
RPDTIFGVTY MVVAPEHPIV SEITTPEQKQ KVEEYQKVSS LKSDLDRMEL TKEKTGVFTG 

       370        380        390        400        410        420 
SFVLNPANPS KEIPIWISDY VLYGYGTGAI MAVPAHDQRD YEFAKTFGLE ILPVIEGEIT 

       430        440        450        460        470        480 
DVAFDSKTSI CIHSSSSEIS IDGLDYSSAS SKIISWAESK RIGKKKTQFK LRDWLFARQR 

       490        500        510        520        530        540 
YWGEPIPLVH YPSGITKPIP ESELPLELPP NLEEFKPSGT GESPLALAKE WLQYKDPETG 

       550        560        570        580        590        600 
EIGTRETNTM PQWAGSCWYY LRYIDPKNGK LFCDPDLEKK WMPVDLYVGG AEHAVLHLLY 

       610        620        630        640        650        660 
SRFWHKFLYD IGVVSTQEPF AKLIHQGLIL GEDKRKMSKS LGNVINPDDV IRKYGADSLR 

       670        680        690        700        710        720 
LFEMFMGPLE MVKPWSTRGV EGVFRFLNRV WRLFHTGEGE SFRLDEIEPT TEELKILHKT 

       730        740        750        760        770        780 
IQKVGEDIPN FSFNTAISQL MIFVNEFTPS DRRPKEVLET FILLLAPFAP HIAEELWKRS 

       790        800        810        820        830        840 
GNNKSLSTEK FPEADPQYLV ESEILIVVQV NGKLRDEFKA PKDVSEADAI SIAKNLDKIK 

       850        860 
GILDGKTIRK EIYVPGKLIN LVIG 

« Hide

References

[1]"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential."
Bulach D.M., Zuerner R.L., Wilson P., Seemann T., McGrath A., Cullen P.A., Davis J., Johnson M., Kuczek E., Alt D.P., Peterson-Burch B., Coppel R.L., Rood J.I., Davies J.K., Adler B.
Proc. Natl. Acad. Sci. U.S.A. 103:14560-14565(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: L550.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000348 Genomic DNA. Translation: ABJ78160.1.
RefSeqYP_797093.1. NC_008508.1.

3D structure databases

ProteinModelPortalQ054T5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING355276.LBL_0571.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ78160; ABJ78160; LBL_0571.
GeneID4408952.
KEGGlbl:LBL_0571.
PATRIC22364442. VBILepBor75619_0694.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycLBOR355276:GHUQ-568-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LEPBL
AccessionPrimary (citable) accession number: Q054T5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries