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Protein

Helicase SWR1

Gene

SWR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling.4 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi721 – 7288ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • helicase activity Source: UniProtKB-KW
  • structural molecule activity Source: SGD

GO - Biological processi

  • chromatin remodeling Source: SGD
  • histone exchange Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29890-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Helicase SWR1 (EC:3.6.4.12)
Alternative name(s):
Swi2/Snf2-related 1
Gene namesi
Name:SWR1
Ordered Locus Names:YDR334W
ORF Names:D9651.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR334W.
SGDiS000002742. SWR1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
  • Swr1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15141514Helicase SWR1PRO_0000074375Add
BLAST

Proteomic databases

MaxQBiQ05471.
TopDownProteomicsiQ05471.

PTM databases

iPTMnetiQ05471.

Interactioni

Subunit structurei

Component of the SWR1 chromatin-remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACT1P6001012EBI-22102,EBI-2169
BDF1P358173EBI-22102,EBI-3493
HTB1P022932EBI-22102,EBI-8088
HTZ1Q126928EBI-22102,EBI-8080
SWC4P532016EBI-22102,EBI-23061

Protein-protein interaction databases

BioGridi32391. 407 interactions.
DIPiDIP-2845N.
IntActiQ05471. 40 interactions.
MINTiMINT-1165514.

Structurei

Secondary structure

1
1514
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi602 – 6054Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M6BX-ray1.78C/F590-639[»]
ProteinModelPortaliQ05471.
SMRiQ05471. Positions 646-1000, 1148-1413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini339 – 41173HSAPROSITE-ProRule annotationAdd
BLAST
Domaini708 – 873166Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini1247 – 1400154Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi824 – 8274DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HSA domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063427.
HOGENOMiHOG000186095.
InParanoidiQ05471.
KOiK11681.
OMAiKIKGREH.
OrthoDBiEOG77T1D0.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00573. HSA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSRKSHAK DKKAGGEQDL ADLKFRYDLL TNELFHLREF VSLVDYDPTH
60 70 80 90 100
FNDSESFQKF LRETHLSLEE RGEKFTDDVA KKGTNGDLTR RRRNLRTSTV
110 120 130 140 150
VSSETTNEKK GDIELKLESI APLVRNKCEE LKYKLSDHSN RKSIVPQKRP
160 170 180 190 200
IQHLKKREAA KSLKFKSERK ENPLPLHEHI AEERYDHIAK VEEPSEAFTI
210 220 230 240 250
KCPSDDSSFE NTSEHYSDNF YFTTSSEEED IKKKRGRKKK KPRIKLVVHP
260 270 280 290 300
PKQTITNPLH VVKPGYESLH EYIASFKSLE DDLTLEEYNK YIDEQRRLLS
310 320 330 340 350
RLKKGIENGA LKYDKETDSL QPITSKEIKT IITYKPDPIS YFYKQQDLQI
360 370 380 390 400
HTDHLINQGI HMSKLFRSST KARIARAKKV SQMIEQHFKH VAGAEERKAK
410 420 430 440 450
EEERHKKSLA RFAVQAVKKR WNMAEKAYRI LRKDEEEQLK RIEGKQHLSK
460 470 480 490 500
MLEKSTQLLE AQLNQVNDDG RSSTPSSDSN DVLSESDDDM DDELSTSSDE
510 520 530 540 550
DEEVDADVGL ENSPASTEAT PTDESLNLIQ LKEKYGHFNG SSTVYDSRNK
560 570 580 590 600
DEKFPTLDKH ESSSSESSVM TGEESSIYSS SENESQNEND RESDDKTPSV
610 620 630 640 650
GLSALFGKGE ESDGDLDLDD SEDFTVNSSS VEGEELEKDQ VDNSAATFER
660 670 680 690 700
AGDFVHTQNE NRDDIKDVEE DAETKVQEEQ LSVVDVPVPS LLRGNLRTYQ
710 720 730 740 750
KQGLNWLASL YNNHTNGILA DEMGLGKTIQ TISLLAYLAC EKENWGPHLI
760 770 780 790 800
VVPTSVLLNW EMEFKRFAPG FKVLTYYGSP QQRKEKRKGW NKPDAFHVCI
810 820 830 840 850
VSYQLVVQDQ HSFKRKRWQY MVLDEAHNIK NFRSTRWQAL LNFNTQRRLL
860 870 880 890 900
LTGTPLQNNL AELWSLLYFL MPQTVIDGKK VSGFADLDAF QQWFGRPVDK
910 920 930 940 950
IIETGQNFGQ DKETKKTVAK LHQVLRPYLL RRLKADVEKQ MPAKYEHIVY
960 970 980 990 1000
CKLSKRQRFL YDDFMSRAQT KATLASGNFM SIVNCLMQLR KVCNHPNLFE
1010 1020 1030 1040 1050
VRPILTSFVL EHCVASDYKD VERTLLKLFK KNNQVNRVDL DFLNLVFTLN
1060 1070 1080 1090 1100
DKDLTSYHAE EISKLTCVKN FVEEVNKLRE TNKQLQEEFG EASFLNFQDA
1110 1120 1130 1140 1150
NQYFKYSNKQ KLEGTVDMLN FLKMVNKLRC DRRPIFGKNL IDLLTKDRRV
1160 1170 1180 1190 1200
KYDKSSIIDN ELIKPLQTRV LDNRKIIDTF AVLTPSAVSL DMRKLALGLN
1210 1220 1230 1240 1250
DDSSVGENTR LKVMQNCFEV SNPLHQLQTK LTIAFPDKSL LQYDCGKLQK
1260 1270 1280 1290 1300
LAILLQQLKD NGHRALIFTQ MTKVLDVLEQ FLNYHGYLYM RLDGATKIED
1310 1320 1330 1340 1350
RQILTERFNT DSRITVFILS SRSGGLGINL TGADTVIFYD SDWNPAMDKQ
1360 1370 1380 1390 1400
CQDRCHRIGQ TRDVHIYRFV SEHTIESNIL KKANQKRQLD NVVIQEGDFT
1410 1420 1430 1440 1450
TDYFSKLSVR DLLGSELPEN ASGGDKPLIA DADVAAKDPR QLERLLAQAE
1460 1470 1480 1490 1500
DEDDVKAANL AMREVEIDND DFDESTEKKA ANEEEENHAE LDEYEGTAHV
1510
DEYMIRFIAN GYYY
Length:1,514
Mass (Da):174,530
Last modified:November 1, 1996 - v1
Checksum:i156E3BB5978905C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51032 Genomic DNA. Translation: AAB64770.1.
BK006938 Genomic DNA. Translation: DAA12176.1.
PIRiS70099.
RefSeqiNP_010621.1. NM_001180642.1.

Genome annotation databases

EnsemblFungiiYDR334W; YDR334W; YDR334W.
GeneIDi851934.
KEGGisce:YDR334W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51032 Genomic DNA. Translation: AAB64770.1.
BK006938 Genomic DNA. Translation: DAA12176.1.
PIRiS70099.
RefSeqiNP_010621.1. NM_001180642.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M6BX-ray1.78C/F590-639[»]
ProteinModelPortaliQ05471.
SMRiQ05471. Positions 646-1000, 1148-1413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32391. 407 interactions.
DIPiDIP-2845N.
IntActiQ05471. 40 interactions.
MINTiMINT-1165514.

PTM databases

iPTMnetiQ05471.

Proteomic databases

MaxQBiQ05471.
TopDownProteomicsiQ05471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR334W; YDR334W; YDR334W.
GeneIDi851934.
KEGGisce:YDR334W.

Organism-specific databases

EuPathDBiFungiDB:YDR334W.
SGDiS000002742. SWR1.

Phylogenomic databases

GeneTreeiENSGT00530000063427.
HOGENOMiHOG000186095.
InParanoidiQ05471.
KOiK11681.
OMAiKIKGREH.
OrthoDBiEOG77T1D0.

Enzyme and pathway databases

BioCyciYEAST:G3O-29890-MONOMER.

Miscellaneous databases

PROiQ05471.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00573. HSA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: FUNCTION OF THE SWR1 COMPLEX, IDENTIFICATION IN THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin."
    Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J.
    PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, IDENTIFICATION BY IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4."
    Krogan N.J., Baetz K., Keogh M.-C., Datta N., Sawa C., Kwok T.C.Y., Thompson N.J., Davey M.G., Pootoolal J., Hughes T.R., Emili A., Buratowski S., Hieter P., Greenblatt J.F.
    Proc. Natl. Acad. Sci. U.S.A. 101:13513-13518(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex."
    Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C.
    Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSWR1_YEAST
AccessioniPrimary (citable) accession number: Q05471
Secondary accession number(s): D6VSW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.