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Protein

L-ascorbate peroxidase 1, cytosolic

Gene

APX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in hydrogen peroxide removal. Constitutes a central component of the reactive oxygen gene network.1 Publication

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei38Transition state stabilizerPROSITE-ProRule annotation1
Active sitei42Proton acceptorPROSITE-ProRule annotation1
Metal bindingi163Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi164Potassium or calciumBy similarity1
Metal bindingi180Potassium or calciumBy similarity1
Metal bindingi182Potassium or calciumBy similarity1
Metal bindingi185Potassium or calcium; via carbonyl oxygenBy similarity1
Metal bindingi187Potassium or calciumBy similarity1

GO - Molecular functioni

  • cytochrome-c peroxidase activity Source: GO_Central
  • heme binding Source: InterPro
  • L-ascorbate peroxidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to oxidative stress Source: GO_Central
  • embryo development ending in seed dormancy Source: TAIR
  • hydrogen peroxide catabolic process Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cytokinin Source: TAIR
  • response to heat Source: TAIR
  • response to reactive oxygen species Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:GQT-2090-MONOMER.
BRENDAi1.11.1.11. 399.

Protein family/group databases

PeroxiBasei1890. AtAPx01.

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase 1, cytosolic (EC:1.11.1.11)
Short name:
AP
Short name:
AtAPx01
Gene namesi
Name:APX1
Ordered Locus Names:At1g07890
ORF Names:F24B9.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G07890.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: TAIR
  • Golgi apparatus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000555922 – 250L-ascorbate peroxidase 1, cytosolicAdd BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei196PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ05431.
PRIDEiQ05431.

2D gel databases

SWISS-2DPAGEQ05431.

PTM databases

iPTMnetiQ05431.

Expressioni

Tissue specificityi

Predominantly expressed in flowers.1 Publication

Inductioni

By ethylene, ozone, sulfur dioxide, Fe exposure, oxidative and heat-shock stresses, and by excess light treatment.4 Publications

Gene expression databases

ExpressionAtlasiQ05431. baseline and differential.
GenevisibleiQ05431. AT.

Interactioni

Protein-protein interaction databases

BioGridi22545. 4 interactors.
IntActiQ05431. 4 interactors.
STRINGi3702.AT1G07890.1.

Structurei

3D structure databases

ProteinModelPortaliQ05431.
SMRiQ05431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IF5T. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ05431.
KOiK00434.
OMAiFFADYAK.
OrthoDBiEOG09360KYY.
PhylomeDBiQ05431.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q05431-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKNYPTVSE DYKKAVEKCR RKLRGLIAEK NCAPIMVRLA WHSAGTFDCQ
60 70 80 90 100
SRTGGPFGTM RFDAEQAHGA NSGIHIALRL LDPIREQFPT ISFADFHQLA
110 120 130 140 150
GVVAVEVTGG PDIPFHPGRE DKPQPPPEGR LPDATKGCDH LRDVFAKQMG
160 170 180 190 200
LSDKDIVALS GAHTLGRCHK DRSGFEGAWT SNPLIFDNSY FKELLSGEKE
210 220 230 240 250
GLLQLVSDKA LLDDPVFRPL VEKYAADEDA FFADYAEAHM KLSELGFADA
Length:250
Mass (Da):27,561
Last modified:January 23, 2007 - v2
Checksum:i33A536D85B2CAA6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59600 mRNA. Translation: CAA42168.1.
D14442 Genomic DNA. Translation: BAA03334.1.
U63815 Genomic DNA. Translation: AAB07880.1.
AC007583 Genomic DNA. Translation: AAF75066.1.
CP002684 Genomic DNA. Translation: AEE28200.1.
CP002684 Genomic DNA. Translation: AEE28201.1.
CP002684 Genomic DNA. Translation: AEE28202.1.
CP002684 Genomic DNA. Translation: AEE28203.1.
CP002684 Genomic DNA. Translation: AEE28204.1.
CP002684 Genomic DNA. Translation: AEE28206.1.
CP002684 Genomic DNA. Translation: AEE28207.1.
AY039879 mRNA. Translation: AAK63983.1.
AY056395 mRNA. Translation: AAL08251.1.
AY094002 mRNA. Translation: AAM16263.1.
AK230096 mRNA. Translation: BAF01915.1.
AY086425 mRNA. Translation: AAM63427.1.
PIRiD86214.
S20866.
RefSeqiNP_001030991.2. NM_001035914.2. [Q05431-1]
NP_001077482.1. NM_001084013.1. [Q05431-1]
NP_001117244.1. NM_001123772.2. [Q05431-1]
NP_172267.1. NM_100663.4. [Q05431-1]
NP_849607.1. NM_179276.2. [Q05431-1]
NP_973786.1. NM_202057.2. [Q05431-1]
UniGeneiAt.47584.
At.67008.

Genome annotation databases

EnsemblPlantsiAT1G07890.1; AT1G07890.1; AT1G07890. [Q05431-1]
AT1G07890.2; AT1G07890.2; AT1G07890. [Q05431-1]
AT1G07890.3; AT1G07890.3; AT1G07890. [Q05431-1]
AT1G07890.4; AT1G07890.4; AT1G07890. [Q05431-1]
AT1G07890.5; AT1G07890.5; AT1G07890. [Q05431-1]
AT1G07890.7; AT1G07890.7; AT1G07890. [Q05431-1]
AT1G07890.8; AT1G07890.8; AT1G07890. [Q05431-1]
GeneIDi837304.
GrameneiAT1G07890.1; AT1G07890.1; AT1G07890.
AT1G07890.2; AT1G07890.2; AT1G07890.
AT1G07890.3; AT1G07890.3; AT1G07890.
AT1G07890.4; AT1G07890.4; AT1G07890.
AT1G07890.5; AT1G07890.5; AT1G07890.
AT1G07890.7; AT1G07890.7; AT1G07890.
AT1G07890.8; AT1G07890.8; AT1G07890.
KEGGiath:AT1G07890.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59600 mRNA. Translation: CAA42168.1.
D14442 Genomic DNA. Translation: BAA03334.1.
U63815 Genomic DNA. Translation: AAB07880.1.
AC007583 Genomic DNA. Translation: AAF75066.1.
CP002684 Genomic DNA. Translation: AEE28200.1.
CP002684 Genomic DNA. Translation: AEE28201.1.
CP002684 Genomic DNA. Translation: AEE28202.1.
CP002684 Genomic DNA. Translation: AEE28203.1.
CP002684 Genomic DNA. Translation: AEE28204.1.
CP002684 Genomic DNA. Translation: AEE28206.1.
CP002684 Genomic DNA. Translation: AEE28207.1.
AY039879 mRNA. Translation: AAK63983.1.
AY056395 mRNA. Translation: AAL08251.1.
AY094002 mRNA. Translation: AAM16263.1.
AK230096 mRNA. Translation: BAF01915.1.
AY086425 mRNA. Translation: AAM63427.1.
PIRiD86214.
S20866.
RefSeqiNP_001030991.2. NM_001035914.2. [Q05431-1]
NP_001077482.1. NM_001084013.1. [Q05431-1]
NP_001117244.1. NM_001123772.2. [Q05431-1]
NP_172267.1. NM_100663.4. [Q05431-1]
NP_849607.1. NM_179276.2. [Q05431-1]
NP_973786.1. NM_202057.2. [Q05431-1]
UniGeneiAt.47584.
At.67008.

3D structure databases

ProteinModelPortaliQ05431.
SMRiQ05431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22545. 4 interactors.
IntActiQ05431. 4 interactors.
STRINGi3702.AT1G07890.1.

Protein family/group databases

PeroxiBasei1890. AtAPx01.

PTM databases

iPTMnetiQ05431.

2D gel databases

SWISS-2DPAGEQ05431.

Proteomic databases

PaxDbiQ05431.
PRIDEiQ05431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G07890.1; AT1G07890.1; AT1G07890. [Q05431-1]
AT1G07890.2; AT1G07890.2; AT1G07890. [Q05431-1]
AT1G07890.3; AT1G07890.3; AT1G07890. [Q05431-1]
AT1G07890.4; AT1G07890.4; AT1G07890. [Q05431-1]
AT1G07890.5; AT1G07890.5; AT1G07890. [Q05431-1]
AT1G07890.7; AT1G07890.7; AT1G07890. [Q05431-1]
AT1G07890.8; AT1G07890.8; AT1G07890. [Q05431-1]
GeneIDi837304.
GrameneiAT1G07890.1; AT1G07890.1; AT1G07890.
AT1G07890.2; AT1G07890.2; AT1G07890.
AT1G07890.3; AT1G07890.3; AT1G07890.
AT1G07890.4; AT1G07890.4; AT1G07890.
AT1G07890.5; AT1G07890.5; AT1G07890.
AT1G07890.7; AT1G07890.7; AT1G07890.
AT1G07890.8; AT1G07890.8; AT1G07890.
KEGGiath:AT1G07890.

Organism-specific databases

TAIRiAT1G07890.

Phylogenomic databases

eggNOGiENOG410IF5T. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ05431.
KOiK00434.
OMAiFFADYAK.
OrthoDBiEOG09360KYY.
PhylomeDBiQ05431.

Enzyme and pathway databases

BioCyciARA:GQT-2090-MONOMER.
BRENDAi1.11.1.11. 399.

Miscellaneous databases

PROiQ05431.

Gene expression databases

ExpressionAtlasiQ05431. baseline and differential.
GenevisibleiQ05431. AT.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPX1_ARATH
AccessioniPrimary (citable) accession number: Q05431
Secondary accession number(s): Q0WLU2, Q2V4P8, Q2V4P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.