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Q05431

- APX1_ARATH

UniProt

Q05431 - APX1_ARATH

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Protein

L-ascorbate peroxidase 1, cytosolic

Gene

APX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a key role in hydrogen peroxide removal. Constitutes a central component of the reactive oxygen gene network.1 Publication

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei38 – 381Transition state stabilizerPROSITE-ProRule annotation
Active sitei42 – 421Proton acceptorPROSITE-ProRule annotation
Metal bindingi163 – 1631Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi164 – 1641Potassium or calciumBy similarity
Metal bindingi180 – 1801Potassium or calciumBy similarity
Metal bindingi182 – 1821Potassium or calciumBy similarity
Metal bindingi185 – 1851Potassium or calcium; via carbonyl oxygenBy similarity
Metal bindingi187 – 1871Potassium or calciumBy similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. L-ascorbate peroxidase activity Source: TAIR
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. embryo development ending in seed dormancy Source: TAIR
  2. hydrogen peroxide catabolic process Source: TAIR
  3. response to cadmium ion Source: TAIR
  4. response to cytokinin Source: TAIR
  5. response to heat Source: TAIR
  6. response to reactive oxygen species Source: TAIR
  7. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:GQT-1075-MONOMER.
ARA:GQT-1077-MONOMER.
ARA:GQT-2090-MONOMER.
ARA:GQT-2091-MONOMER.
ARA:GQT-2092-MONOMER.
ARA:GQT-2093-MONOMER.
ARA:GQT-2094-MONOMER.

Protein family/group databases

PeroxiBasei1890. AtAPx01.

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase 1, cytosolic (EC:1.11.1.11)
Short name:
AP
Short name:
AtAPx01
Gene namesi
Name:APX1
Ordered Locus Names:At1g07890
ORF Names:F24B9.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G07890.

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. chloroplast Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. cytosol Source: TAIR
  5. Golgi apparatus Source: TAIR
  6. plasma membrane Source: TAIR
  7. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 250249L-ascorbate peroxidase 1, cytosolicPRO_0000055592Add
BLAST

Proteomic databases

PaxDbiQ05431.
PRIDEiQ05431.

2D gel databases

SWISS-2DPAGEQ05431.

Expressioni

Tissue specificityi

Predominantly expressed in flowers.1 Publication

Inductioni

By ethylene, ozone, sulfur dioxide, Fe exposure, oxidative and heat-shock stresses, and by excess light treatment.4 Publications

Gene expression databases

ExpressionAtlasiQ05431. baseline and differential.
GenevestigatoriQ05431.

Interactioni

Protein-protein interaction databases

BioGridi22545. 3 interactions.
IntActiQ05431. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ05431.
SMRiQ05431. Positions 3-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0376.
HOGENOMiHOG000189824.
InParanoidiQ05431.
KOiK00434.
OMAiPTVSEDY.
PhylomeDBiQ05431.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q05431-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKNYPTVSE DYKKAVEKCR RKLRGLIAEK NCAPIMVRLA WHSAGTFDCQ
60 70 80 90 100
SRTGGPFGTM RFDAEQAHGA NSGIHIALRL LDPIREQFPT ISFADFHQLA
110 120 130 140 150
GVVAVEVTGG PDIPFHPGRE DKPQPPPEGR LPDATKGCDH LRDVFAKQMG
160 170 180 190 200
LSDKDIVALS GAHTLGRCHK DRSGFEGAWT SNPLIFDNSY FKELLSGEKE
210 220 230 240 250
GLLQLVSDKA LLDDPVFRPL VEKYAADEDA FFADYAEAHM KLSELGFADA
Length:250
Mass (Da):27,561
Last modified:January 23, 2007 - v2
Checksum:i33A536D85B2CAA6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59600 mRNA. Translation: CAA42168.1.
D14442 Genomic DNA. Translation: BAA03334.1.
U63815 Genomic DNA. Translation: AAB07880.1.
AC007583 Genomic DNA. Translation: AAF75066.1.
CP002684 Genomic DNA. Translation: AEE28200.1.
CP002684 Genomic DNA. Translation: AEE28201.1.
CP002684 Genomic DNA. Translation: AEE28202.1.
CP002684 Genomic DNA. Translation: AEE28203.1.
CP002684 Genomic DNA. Translation: AEE28204.1.
CP002684 Genomic DNA. Translation: AEE28206.1.
CP002684 Genomic DNA. Translation: AEE28207.1.
AY039879 mRNA. Translation: AAK63983.1.
AY056395 mRNA. Translation: AAL08251.1.
AY094002 mRNA. Translation: AAM16263.1.
AK230096 mRNA. Translation: BAF01915.1.
AY086425 mRNA. Translation: AAM63427.1.
PIRiD86214.
S20866.
RefSeqiNP_001030991.2. NM_001035914.2. [Q05431-1]
NP_001030992.2. NM_001035915.2. [Q05431-1]
NP_001077482.1. NM_001084013.1. [Q05431-1]
NP_001117244.1. NM_001123772.1. [Q05431-1]
NP_172267.1. NM_100663.3. [Q05431-1]
NP_849607.1. NM_179276.2. [Q05431-1]
NP_973786.1. NM_202057.2. [Q05431-1]
UniGeneiAt.47584.
At.67008.

Genome annotation databases

EnsemblPlantsiAT1G07890.1; AT1G07890.1; AT1G07890. [Q05431-1]
AT1G07890.2; AT1G07890.2; AT1G07890. [Q05431-1]
AT1G07890.3; AT1G07890.3; AT1G07890. [Q05431-1]
AT1G07890.4; AT1G07890.4; AT1G07890. [Q05431-1]
AT1G07890.5; AT1G07890.5; AT1G07890. [Q05431-1]
AT1G07890.7; AT1G07890.7; AT1G07890. [Q05431-1]
AT1G07890.8; AT1G07890.8; AT1G07890. [Q05431-1]
GeneIDi837304.
KEGGiath:AT1G07890.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59600 mRNA. Translation: CAA42168.1 .
D14442 Genomic DNA. Translation: BAA03334.1 .
U63815 Genomic DNA. Translation: AAB07880.1 .
AC007583 Genomic DNA. Translation: AAF75066.1 .
CP002684 Genomic DNA. Translation: AEE28200.1 .
CP002684 Genomic DNA. Translation: AEE28201.1 .
CP002684 Genomic DNA. Translation: AEE28202.1 .
CP002684 Genomic DNA. Translation: AEE28203.1 .
CP002684 Genomic DNA. Translation: AEE28204.1 .
CP002684 Genomic DNA. Translation: AEE28206.1 .
CP002684 Genomic DNA. Translation: AEE28207.1 .
AY039879 mRNA. Translation: AAK63983.1 .
AY056395 mRNA. Translation: AAL08251.1 .
AY094002 mRNA. Translation: AAM16263.1 .
AK230096 mRNA. Translation: BAF01915.1 .
AY086425 mRNA. Translation: AAM63427.1 .
PIRi D86214.
S20866.
RefSeqi NP_001030991.2. NM_001035914.2. [Q05431-1 ]
NP_001030992.2. NM_001035915.2. [Q05431-1 ]
NP_001077482.1. NM_001084013.1. [Q05431-1 ]
NP_001117244.1. NM_001123772.1. [Q05431-1 ]
NP_172267.1. NM_100663.3. [Q05431-1 ]
NP_849607.1. NM_179276.2. [Q05431-1 ]
NP_973786.1. NM_202057.2. [Q05431-1 ]
UniGenei At.47584.
At.67008.

3D structure databases

ProteinModelPortali Q05431.
SMRi Q05431. Positions 3-250.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 22545. 3 interactions.
IntActi Q05431. 4 interactions.

Protein family/group databases

PeroxiBasei 1890. AtAPx01.

2D gel databases

SWISS-2DPAGE Q05431.

Proteomic databases

PaxDbi Q05431.
PRIDEi Q05431.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G07890.1 ; AT1G07890.1 ; AT1G07890 . [Q05431-1 ]
AT1G07890.2 ; AT1G07890.2 ; AT1G07890 . [Q05431-1 ]
AT1G07890.3 ; AT1G07890.3 ; AT1G07890 . [Q05431-1 ]
AT1G07890.4 ; AT1G07890.4 ; AT1G07890 . [Q05431-1 ]
AT1G07890.5 ; AT1G07890.5 ; AT1G07890 . [Q05431-1 ]
AT1G07890.7 ; AT1G07890.7 ; AT1G07890 . [Q05431-1 ]
AT1G07890.8 ; AT1G07890.8 ; AT1G07890 . [Q05431-1 ]
GeneIDi 837304.
KEGGi ath:AT1G07890.

Organism-specific databases

GeneFarmi 1942. 146.
TAIRi AT1G07890.

Phylogenomic databases

eggNOGi COG0376.
HOGENOMi HOG000189824.
InParanoidi Q05431.
KOi K00434.
OMAi PTVSEDY.
PhylomeDBi Q05431.

Enzyme and pathway databases

BioCyci ARA:GQT-1075-MONOMER.
ARA:GQT-1077-MONOMER.
ARA:GQT-2090-MONOMER.
ARA:GQT-2091-MONOMER.
ARA:GQT-2092-MONOMER.
ARA:GQT-2093-MONOMER.
ARA:GQT-2094-MONOMER.

Gene expression databases

ExpressionAtlasi Q05431. baseline and differential.
Genevestigatori Q05431.

Family and domain databases

InterProi IPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view ]
Pfami PF00141. peroxidase. 1 hit.
[Graphical view ]
PRINTSi PR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMi SSF48113. SSF48113. 1 hit.
PROSITEi PS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a cDNA encoding ascorbate peroxidase from Arabidopsis thaliana."
    Kubo A., Saji H., Tanaka K., Tanaka K., Kondo N.
    Plant Mol. Biol. 18:691-701(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-17.
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Genomic DNA structure of a gene encoding cytosolic ascorbate peroxidase from Arabidopsis thaliana."
    Kubo A., Saji H., Tanaka K., Kondo N.
    FEBS Lett. 315:313-317(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  3. Tremousaygue D., Bardet C., Dabos P., Regad F., Pelese F., Lescure B.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  4. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  9. "Expression of Arabidopsis cytosolic ascorbate peroxidase gene in response to ozone or sulfur dioxide."
    Kubo A., Saji H., Tanaka K., Kondo N.
    Plant Mol. Biol. 29:479-489(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress."
    Karpinski S., Escobar C., Karpinski B., Creissen G.P., Mullineaux P.M.
    Plant Cell 9:627-640(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  11. "The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter."
    Storozhenko S., De Pauw P., Van Montagu M., Inze D., Kushnir S.
    Plant Physiol. 118:1005-1014(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  12. "Iron-regulated expression of a cytosolic ascorbate peroxidase encoded by the APX1 gene in Arabidopsis seedlings."
    Fourcroy P., Vansuyt G., Kushnir S., Inze D., Briat J.-F.
    Plant Physiol. 134:605-613(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  13. "Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis."
    Davletova S., Rizhsky L., Liang H., Shengqiang Z., Oliver D.J., Coutu J., Shulaev V., Schlauch K., Mittler R.
    Plant Cell 17:268-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiAPX1_ARATH
AccessioniPrimary (citable) accession number: Q05431
Secondary accession number(s): Q0WLU2, Q2V4P8, Q2V4P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3