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Protein

Cytochrome P450 2E1

Gene

Cyp2e1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.By similarity

Catalytic activityi

4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: GO_Central
  2. enzyme binding Source: MGI
  3. heme binding Source: UniProtKB
  4. iron ion binding Source: InterPro
  5. monooxygenase activity Source: MGI
  6. oxidoreductase activity Source: MGI
  7. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB-EC
  8. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  9. oxygen binding Source: GO_Central
  10. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. drug metabolic process Source: MGI
  2. epoxygenase P450 pathway Source: GO_Central
  3. exogenous drug catabolic process Source: GO_Central
  4. heterocycle metabolic process Source: MGI
  5. monoterpenoid metabolic process Source: MGI
  6. oxidation-reduction process Source: MGI
  7. response to ethanol Source: Ensembl
  8. response to organonitrogen compound Source: Ensembl
  9. response to ozone Source: Ensembl
  10. steroid metabolic process Source: MGI
  11. triglyceride metabolic process Source: Ensembl
  12. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12920.
ReactomeiREACT_262340. CYP2E1 reactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2E1 (EC:1.14.13.-)
Alternative name(s):
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7)
CYPIIE1
Cytochrome P450-ALC
Cytochrome P450-J
Gene namesi
Name:Cyp2e1
Synonyms:Cyp2e, Cyp2e-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:88607. Cyp2e1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. Golgi membrane Source: Ensembl
  3. intrinsic component of endoplasmic reticulum membrane Source: Ensembl
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 493492Cytochrome P450 2E1PRO_0000051755Add
BLAST

Proteomic databases

MaxQBiQ05421.
PaxDbiQ05421.
PRIDEiQ05421.

PTM databases

PhosphoSiteiQ05421.

Expressioni

Tissue specificityi

Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.

Developmental stagei

Detectable in the female liver on day 1 and reaches steady state levels on days 16-20.

Inductioni

By ethanol and acetone in the liver and by testosterone in the kidney and adrenal tissues.

Gene expression databases

BgeeiQ05421.
GenevestigatoriQ05421.

Interactioni

Protein-protein interaction databases

IntActiQ05421. 2 interactions.
MINTiMINT-1862880.

Structurei

3D structure databases

ProteinModelPortaliQ05421.
SMRiQ05421. Positions 32-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni298 – 3036Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiQ05421.
KOiK07415.
OMAiPMYTMEN.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ05421.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01687. EP450ICYP2E.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05421-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVLGITVAL LVWIATLLLV SIWKQIYRSW NLPPGPFPIP FFGNIFQLDL
60 70 80 90 100
KDIPKSLTKL AKRFGPVFTL HLGQRRIVVL HGYKAVKEVL LNHKNEFSGR
110 120 130 140 150
GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE
160 170 180 190 200
AHFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YDDKKCLELM
210 220 230 240 250
SLFNENFYLL STPWIQAYNY FSDYLQYLPG SHRKVMKNVS EIRQYTLGKA
260 270 280 290 300
KEHLKSLDIN CPRDVTDCLL IEMEKEKHSQ EPMYTMENIS VTLADLFFAG
310 320 330 340 350
TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRAPA VRDRMNMPYM
360 370 380 390 400
DAVVHEIQRF INLVPSNLPH EATRDTVFRG YVIPKGTVVI PTLDSLLFDN
410 420 430 440 450
YEFPDPETFK PEHFLNENGK FKYSDYFKAF SAGKRVCVGE GLARMELFLL
460 470 480 490
LSAILQHFNL KSLVDPKDID LSPVTIGFGS IPREFKLCVI PRS
Length:493
Mass (Da):56,805
Last modified:February 1, 1994 - v1
Checksum:i4031AB016DA56A9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11650 mRNA. Translation: AAA39879.1.
X62595 mRNA. Translation: CAA44481.1.
BC013451 mRNA. Translation: AAH13451.1.
M54877 mRNA. Translation: AAA37275.1.
X01026 mRNA. Translation: CAA25510.1. Sequence problems.
CCDSiCCDS21985.1.
PIRiA47350.
S19657. A21231.
RefSeqiNP_067257.1. NM_021282.2.
UniGeneiMm.21758.

Genome annotation databases

EnsembliENSMUST00000026552; ENSMUSP00000026552; ENSMUSG00000025479.
GeneIDi13106.
KEGGimmu:13106.
UCSCiuc009kic.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11650 mRNA. Translation: AAA39879.1.
X62595 mRNA. Translation: CAA44481.1.
BC013451 mRNA. Translation: AAH13451.1.
M54877 mRNA. Translation: AAA37275.1.
X01026 mRNA. Translation: CAA25510.1. Sequence problems.
CCDSiCCDS21985.1.
PIRiA47350.
S19657. A21231.
RefSeqiNP_067257.1. NM_021282.2.
UniGeneiMm.21758.

3D structure databases

ProteinModelPortaliQ05421.
SMRiQ05421. Positions 32-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ05421. 2 interactions.
MINTiMINT-1862880.

PTM databases

PhosphoSiteiQ05421.

Proteomic databases

MaxQBiQ05421.
PaxDbiQ05421.
PRIDEiQ05421.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026552; ENSMUSP00000026552; ENSMUSG00000025479.
GeneIDi13106.
KEGGimmu:13106.
UCSCiuc009kic.1. mouse.

Organism-specific databases

CTDi1571.
MGIiMGI:88607. Cyp2e1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiQ05421.
KOiK07415.
OMAiPMYTMEN.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ05421.
TreeFamiTF352043.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12920.
ReactomeiREACT_262340. CYP2E1 reactions.

Miscellaneous databases

ChiTaRSiCyp2e1. mouse.
NextBioi283102.
PROiQ05421.
SOURCEiSearch...

Gene expression databases

BgeeiQ05421.
GenevestigatoriQ05421.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01687. EP450ICYP2E.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse ethanol-inducible cytochrome P-450 (P450IIE1). Characterization of cDNA clones and testosterone induction in kidney tissue."
    Davis J.F., Felder M.R.
    J. Biol. Chem. 268:16584-16589(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6S.
    Tissue: Liver.
  2. "cDNA sequence, deduced amino acid sequence, predicted gene structure and chemical regulation of mouse Cyp2e1."
    Freeman J.E., Stirling D., Russel A.L., Wolf C.R.
    Biochem. J. 281:689-695(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Primary structure of the repeating element B2 and of the adjoining sequences in cloned mRNA actively transcribing in mouse liver cells."
    Ivanov P.L., Ryskov A.P., Kramerov D.A., Georgiev G.P.
    Dokl. Akad. Nauk SSSR 269:227-230(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
    Tissue: Liver.
  5. "Mouse ubiquitous B2 repeat in polysomal and cytoplasmic poly(A)+RNAs: unidirectional orientation and 3'-end localization."
    Ryskov A.P., Ivanov P.L., Kramerov D.A., Georgiev G.P.
    Nucleic Acids Res. 11:6541-6558(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
  6. "Universal orientation and 3' terminal localization of repetitive sequences of the B2 family in mRNA."
    Ryskov A.P., Ivanov P.L., Kramerov D.A., Georgiev G.P.
    Mol. Biol. (Mosk.) 18:74-83(1984)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.

Entry informationi

Entry nameiCP2E1_MOUSE
AccessioniPrimary (citable) accession number: Q05421
Secondary accession number(s): Q9Z198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 4, 2015
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.