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Q05421

- CP2E1_MOUSE

UniProt

Q05421 - CP2E1_MOUSE

Protein

Cytochrome P450 2E1

Gene

Cyp2e1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.By similarity

    Catalytic activityi

    4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: UniProtKB
    2. iron ion binding Source: InterPro
    3. monooxygenase activity Source: MGI
    4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB-EC
    5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: Ensembl

    GO - Biological processi

    1. drug metabolic process Source: Ensembl
    2. heterocycle metabolic process Source: Ensembl
    3. monoterpenoid metabolic process Source: Ensembl
    4. response to drug Source: Ensembl
    5. response to ethanol Source: Ensembl
    6. response to organonitrogen compound Source: Ensembl
    7. response to ozone Source: Ensembl
    8. steroid metabolic process Source: Ensembl
    9. triglyceride metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12920.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2E1 (EC:1.14.13.-)
    Alternative name(s):
    4-nitrophenol 2-hydroxylase (EC:1.14.13.n7)
    CYPIIE1
    Cytochrome P450-ALC
    Cytochrome P450-J
    Gene namesi
    Name:Cyp2e1
    Synonyms:Cyp2e, Cyp2e-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:88607. Cyp2e1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI
    2. Golgi membrane Source: Ensembl
    3. intrinsic component of endoplasmic reticulum membrane Source: Ensembl
    4. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 493492Cytochrome P450 2E1PRO_0000051755Add
    BLAST

    Proteomic databases

    MaxQBiQ05421.
    PaxDbiQ05421.
    PRIDEiQ05421.

    PTM databases

    PhosphoSiteiQ05421.

    Expressioni

    Tissue specificityi

    Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.

    Developmental stagei

    Detectable in the female liver on day 1 and reaches steady state levels on days 16-20.

    Inductioni

    By ethanol and acetone in the liver and by testosterone in the kidney and adrenal tissues.

    Gene expression databases

    BgeeiQ05421.
    GenevestigatoriQ05421.

    Interactioni

    Protein-protein interaction databases

    IntActiQ05421. 2 interactions.
    MINTiMINT-1862880.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05421.
    SMRiQ05421. Positions 32-493.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni298 – 3036Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00680000099783.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiQ05421.
    KOiK07415.
    OMAiPMYTMEN.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiQ05421.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01687. EP450ICYP2E.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05421-1 [UniParc]FASTAAdd to Basket

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    MAVLGITVAL LVWIATLLLV SIWKQIYRSW NLPPGPFPIP FFGNIFQLDL    50
    KDIPKSLTKL AKRFGPVFTL HLGQRRIVVL HGYKAVKEVL LNHKNEFSGR 100
    GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE 150
    AHFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YDDKKCLELM 200
    SLFNENFYLL STPWIQAYNY FSDYLQYLPG SHRKVMKNVS EIRQYTLGKA 250
    KEHLKSLDIN CPRDVTDCLL IEMEKEKHSQ EPMYTMENIS VTLADLFFAG 300
    TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRAPA VRDRMNMPYM 350
    DAVVHEIQRF INLVPSNLPH EATRDTVFRG YVIPKGTVVI PTLDSLLFDN 400
    YEFPDPETFK PEHFLNENGK FKYSDYFKAF SAGKRVCVGE GLARMELFLL 450
    LSAILQHFNL KSLVDPKDID LSPVTIGFGS IPREFKLCVI PRS 493
    Length:493
    Mass (Da):56,805
    Last modified:February 1, 1994 - v1
    Checksum:i4031AB016DA56A9C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11650 mRNA. Translation: AAA39879.1.
    X62595 mRNA. Translation: CAA44481.1.
    BC013451 mRNA. Translation: AAH13451.1.
    M54877 mRNA. Translation: AAA37275.1.
    X01026 mRNA. Translation: CAA25510.1. Sequence problems.
    CCDSiCCDS21985.1.
    PIRiA47350.
    S19657. A21231.
    RefSeqiNP_067257.1. NM_021282.2.
    UniGeneiMm.21758.

    Genome annotation databases

    EnsembliENSMUST00000026552; ENSMUSP00000026552; ENSMUSG00000025479.
    GeneIDi13106.
    KEGGimmu:13106.
    UCSCiuc009kic.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11650 mRNA. Translation: AAA39879.1 .
    X62595 mRNA. Translation: CAA44481.1 .
    BC013451 mRNA. Translation: AAH13451.1 .
    M54877 mRNA. Translation: AAA37275.1 .
    X01026 mRNA. Translation: CAA25510.1 . Sequence problems.
    CCDSi CCDS21985.1.
    PIRi A47350.
    S19657. A21231.
    RefSeqi NP_067257.1. NM_021282.2.
    UniGenei Mm.21758.

    3D structure databases

    ProteinModelPortali Q05421.
    SMRi Q05421. Positions 32-493.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q05421. 2 interactions.
    MINTi MINT-1862880.

    PTM databases

    PhosphoSitei Q05421.

    Proteomic databases

    MaxQBi Q05421.
    PaxDbi Q05421.
    PRIDEi Q05421.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026552 ; ENSMUSP00000026552 ; ENSMUSG00000025479 .
    GeneIDi 13106.
    KEGGi mmu:13106.
    UCSCi uc009kic.1. mouse.

    Organism-specific databases

    CTDi 1571.
    MGIi MGI:88607. Cyp2e1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00680000099783.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi Q05421.
    KOi K07415.
    OMAi PMYTMEN.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi Q05421.
    TreeFami TF352043.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-12920.

    Miscellaneous databases

    ChiTaRSi CYP2E1. mouse.
    NextBioi 283102.
    PROi Q05421.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q05421.
    Genevestigatori Q05421.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01687. EP450ICYP2E.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse ethanol-inducible cytochrome P-450 (P450IIE1). Characterization of cDNA clones and testosterone induction in kidney tissue."
      Davis J.F., Felder M.R.
      J. Biol. Chem. 268:16584-16589(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6S.
      Tissue: Liver.
    2. "cDNA sequence, deduced amino acid sequence, predicted gene structure and chemical regulation of mouse Cyp2e1."
      Freeman J.E., Stirling D., Russel A.L., Wolf C.R.
      Biochem. J. 281:689-695(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    4. "Primary structure of the repeating element B2 and of the adjoining sequences in cloned mRNA actively transcribing in mouse liver cells."
      Ivanov P.L., Ryskov A.P., Kramerov D.A., Georgiev G.P.
      Dokl. Akad. Nauk SSSR 269:227-230(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
      Tissue: Liver.
    5. "Mouse ubiquitous B2 repeat in polysomal and cytoplasmic poly(A)+RNAs: unidirectional orientation and 3'-end localization."
      Ryskov A.P., Ivanov P.L., Kramerov D.A., Georgiev G.P.
      Nucleic Acids Res. 11:6541-6558(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
    6. "Universal orientation and 3' terminal localization of repetitive sequences of the B2 family in mRNA."
      Ryskov A.P., Ivanov P.L., Kramerov D.A., Georgiev G.P.
      Mol. Biol. (Mosk.) 18:74-83(1984)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.

    Entry informationi

    Entry nameiCP2E1_MOUSE
    AccessioniPrimary (citable) accession number: Q05421
    Secondary accession number(s): Q9Z198
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3