Q05421 (CP2E1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2E1
EC=1.14.13.-

Alternative name(s):
4-nitrophenol 2-hydroxylase
EC=1.14.13.n7
CYPIIE1
Cytochrome P450-ALC
Cytochrome P450-J

Gene names

Name:	Cyp2e1
Synonyms:	Cyp2e, Cyp2e-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites By similarity.
Catalytic activity	4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.
Developmental stage	Detectable in the female liver on day 1 and reaches steady state levels on days 16-20.
Induction	By ethanol and acetone in the liver and by testosterone in the kidney and adrenal tissues.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	drug metabolic process Inferred from electronic annotation. Source: Compara heterocycle metabolic process Inferred from electronic annotation. Source: Compara monoterpenoid metabolic process Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to organic nitrogen Inferred from electronic annotation. Source: Compara response to ozone Inferred from electronic annotation. Source: Compara steroid metabolic process Inferred from electronic annotation. Source: Compara triglyceride metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: Compara endoplasmic reticulum Inferred from direct assay PubMed 16223484. Source: MGI intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: Compara mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from sequence or structural similarity. Source: UniProtKB iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from direct assay PubMed 14642533. Source: MGI oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 493	492	Cytochrome P450 2E1		PRO_0000051755
Regions
	Region	298 – 303	6	Substrate binding By similarity
Sites
	Metal binding	437	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	129	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q05421 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 4031AB016DA56A9C	FASTA	493	56,805
	10 20 30 40 50 60 MAVLGITVAL LVWIATLLLV SIWKQIYRSW NLPPGPFPIP FFGNIFQLDL KDIPKSLTKL 70 80 90 100 110 120 AKRFGPVFTL HLGQRRIVVL HGYKAVKEVL LNHKNEFSGR GDIPVFQEYK NKGIIFNNGP 130 140 150 160 170 180 TWKDVRRFSL SILRDWGMGK QGNEARIQRE AHFLVEELKK TKGQPFDPTF LIGCAPCNVI 190 200 210 220 230 240 ADILFNKRFD YDDKKCLELM SLFNENFYLL STPWIQAYNY FSDYLQYLPG SHRKVMKNVS 250 260 270 280 290 300 EIRQYTLGKA KEHLKSLDIN CPRDVTDCLL IEMEKEKHSQ EPMYTMENIS VTLADLFFAG 310 320 330 340 350 360 TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRAPA VRDRMNMPYM DAVVHEIQRF 370 380 390 400 410 420 INLVPSNLPH EATRDTVFRG YVIPKGTVVI PTLDSLLFDN YEFPDPETFK PEHFLNENGK 430 440 450 460 470 480 FKYSDYFKAF SAGKRVCVGE GLARMELFLL LSAILQHFNL KSLVDPKDID LSPVTIGFGS 490 IPREFKLCVI PRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse ethanol-inducible cytochrome P-450 (P450IIE1). Characterization of cDNA clones and testosterone induction in kidney tissue." Davis J.F., Felder M.R. J. Biol. Chem. 268:16584-16589(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6S. Tissue: Liver.
[2]	"cDNA sequence, deduced amino acid sequence, predicted gene structure and chemical regulation of mouse Cyp2e1." Freeman J.E., Stirling D., Russel A.L., Wolf C.R. Biochem. J. 281:689-695(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[4]	"Primary structure of the repeating element B2 and of the adjoining sequences in cloned mRNA actively transcribing in mouse liver cells." Ivanov P.L., Ryskov A.P., Kramerov D.A., Georgiev G.P. Dokl. Akad. Nauk SSSR 269:227-230(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493. Tissue: Liver.
[5]	"Mouse ubiquitous B2 repeat in polysomal and cytoplasmic poly(A)+RNAs: unidirectional orientation and 3'-end localization." Ryskov A.P., Ivanov P.L., Kramerov D.A., Georgiev G.P. Nucleic Acids Res. 11:6541-6558(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
[6]	"Universal orientation and 3' terminal localization of repetitive sequences of the B2 family in mRNA." Ryskov A.P., Ivanov P.L., Kramerov D.A., Georgiev G.P. Mol. Biol. (Mosk.) 18:74-83(1984) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
[7]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11650 mRNA. Translation: AAA39879.1. X62595 mRNA. Translation: CAA44481.1. BC013451 mRNA. Translation: AAH13451.1. M54877 mRNA. Translation: AAA37275.1. X01026 mRNA. Translation: CAA25510.1. Sequence problems.
IPI	IPI00110556.
PIR	A47350. A21231. S19657.
RefSeq	NP_067257.1. NM_021282.2.
UniGene	Mm.21758. Mm.490249.
3D structure databases
ProteinModelPortal	Q05421.
SMR	Q05421. Positions 32-493.
ModBase	Search...
PTM databases
PhosphoSite	Q05421.
Proteomic databases
PaxDb	Q05421.
PRIDE	Q05421.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026552; ENSMUSP00000026552; ENSMUSG00000025479.
GeneID	13106.
KEGG	mmu:13106.
UCSC	uc009kic.1. mouse.
Organism-specific databases
CTD	1571.
MGI	MGI:88607. Cyp2e1.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00680000099783.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
InParanoid	Q05421.
KO	K07415.
OMA	PMYTMEN.
OrthoDB	EOG48WC22.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12920.
Gene expression databases
Bgee	Q05421.
Genevestigator	Q05421.
GermOnline	ENSMUSG00000025479. Mus musculus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008070. Cyt_P450_E_grp-I_CYP2E-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01687. EP450ICYP2E. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CYP2E1. mouse.
NextBio	283102.
SOURCE	Search...

Entry information

Entry name

CP2E1_MOUSE

Accession

Primary (citable) accession number: Q05421
Secondary accession number(s): Q9Z198

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program