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Protein

Cytochrome P450 2E1

Gene

Cyp2e1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.By similarity

Catalytic activityi

4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi437Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12920.
ReactomeiR-MMU-211999. CYP2E1 reactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2E1 (EC:1.14.13.-)
Alternative name(s):
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7)
CYPIIE1
Cytochrome P450-ALC
Cytochrome P450-J
Gene namesi
Name:Cyp2e1
Synonyms:Cyp2e, Cyp2e-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:88607. Cyp2e1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000517551 – 493Cytochrome P450 2E1Add BLAST493

Proteomic databases

MaxQBiQ05421.
PaxDbiQ05421.
PeptideAtlasiQ05421.
PRIDEiQ05421.

PTM databases

iPTMnetiQ05421.
PhosphoSitePlusiQ05421.
SwissPalmiQ05421.

Expressioni

Tissue specificityi

Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.

Developmental stagei

Detectable in the female liver on day 1 and reaches steady state levels on days 16-20.

Inductioni

By ethanol and acetone in the liver and by testosterone in the kidney and adrenal tissues.

Gene expression databases

BgeeiENSMUSG00000025479.
ExpressionAtlasiQ05421. baseline and differential.
GenevisibleiQ05421. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ05421. 2 interactors.
MINTiMINT-1862880.
STRINGi10090.ENSMUSP00000026552.

Structurei

3D structure databases

ProteinModelPortaliQ05421.
SMRiQ05421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni298 – 303Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00880000137861.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiQ05421.
KOiK07415.
OMAiHEATQDT.
OrthoDBiEOG091G0BT8.
PhylomeDBiQ05421.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
IPR036396. Cyt_P450_sf.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR01687. EP450ICYP2E.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05421-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVLGITVAL LVWIATLLLV SIWKQIYRSW NLPPGPFPIP FFGNIFQLDL
60 70 80 90 100
KDIPKSLTKL AKRFGPVFTL HLGQRRIVVL HGYKAVKEVL LNHKNEFSGR
110 120 130 140 150
GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE
160 170 180 190 200
AHFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YDDKKCLELM
210 220 230 240 250
SLFNENFYLL STPWIQAYNY FSDYLQYLPG SHRKVMKNVS EIRQYTLGKA
260 270 280 290 300
KEHLKSLDIN CPRDVTDCLL IEMEKEKHSQ EPMYTMENIS VTLADLFFAG
310 320 330 340 350
TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRAPA VRDRMNMPYM
360 370 380 390 400
DAVVHEIQRF INLVPSNLPH EATRDTVFRG YVIPKGTVVI PTLDSLLFDN
410 420 430 440 450
YEFPDPETFK PEHFLNENGK FKYSDYFKAF SAGKRVCVGE GLARMELFLL
460 470 480 490
LSAILQHFNL KSLVDPKDID LSPVTIGFGS IPREFKLCVI PRS
Length:493
Mass (Da):56,805
Last modified:February 1, 1994 - v1
Checksum:i4031AB016DA56A9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11650 mRNA. Translation: AAA39879.1.
X62595 mRNA. Translation: CAA44481.1.
BC013451 mRNA. Translation: AAH13451.1.
M54877 mRNA. Translation: AAA37275.1.
X01026 mRNA. Translation: CAA25510.1. Sequence problems.
CCDSiCCDS21985.1.
PIRiA47350.
S19657. A21231.
RefSeqiNP_067257.1. NM_021282.2.
UniGeneiMm.21758.

Genome annotation databases

EnsembliENSMUST00000026552; ENSMUSP00000026552; ENSMUSG00000025479.
GeneIDi13106.
KEGGimmu:13106.
UCSCiuc009kic.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCP2E1_MOUSE
AccessioniPrimary (citable) accession number: Q05421
Secondary accession number(s): Q9Z198
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 25, 2017
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families