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Q05421 (CP2E1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2E1

EC=1.14.13.-
Alternative name(s):
4-nitrophenol 2-hydroxylase
EC=1.14.13.n7
CYPIIE1
Cytochrome P450-ALC
Cytochrome P450-J
Gene names
Name:Cyp2e1
Synonyms:Cyp2e, Cyp2e-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites By similarity.

Catalytic activity

4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.

Developmental stage

Detectable in the female liver on day 1 and reaches steady state levels on days 16-20.

Induction

By ethanol and acetone in the liver and by testosterone in the kidney and adrenal tissues.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdrug metabolic process

Inferred from electronic annotation. Source: Ensembl

heterocycle metabolic process

Inferred from electronic annotation. Source: Ensembl

monoterpenoid metabolic process

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to organonitrogen compound

Inferred from electronic annotation. Source: Ensembl

response to ozone

Inferred from electronic annotation. Source: Ensembl

steroid metabolic process

Inferred from electronic annotation. Source: Ensembl

triglyceride metabolic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum

Inferred from direct assay PubMed 16223484. Source: MGI

intrinsic component of endoplasmic reticulum membrane

Inferred from electronic annotation. Source: Ensembl

mitochondrion

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionheme binding

Inferred from sequence or structural similarity. Source: UniProtKB

iron ion binding

Inferred from electronic annotation. Source: InterPro

monooxygenase activity

Inferred from direct assay PubMed 14642533. Source: MGI

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Inferred from electronic annotation. Source: UniProtKB-EC

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 493492Cytochrome P450 2E1
PRO_0000051755

Regions

Region298 – 3036Substrate binding By similarity

Sites

Metal binding4371Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05421 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 4031AB016DA56A9C

FASTA49356,805
        10         20         30         40         50         60 
MAVLGITVAL LVWIATLLLV SIWKQIYRSW NLPPGPFPIP FFGNIFQLDL KDIPKSLTKL 

        70         80         90        100        110        120 
AKRFGPVFTL HLGQRRIVVL HGYKAVKEVL LNHKNEFSGR GDIPVFQEYK NKGIIFNNGP 

       130        140        150        160        170        180 
TWKDVRRFSL SILRDWGMGK QGNEARIQRE AHFLVEELKK TKGQPFDPTF LIGCAPCNVI 

       190        200        210        220        230        240 
ADILFNKRFD YDDKKCLELM SLFNENFYLL STPWIQAYNY FSDYLQYLPG SHRKVMKNVS 

       250        260        270        280        290        300 
EIRQYTLGKA KEHLKSLDIN CPRDVTDCLL IEMEKEKHSQ EPMYTMENIS VTLADLFFAG 

       310        320        330        340        350        360 
TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRAPA VRDRMNMPYM DAVVHEIQRF 

       370        380        390        400        410        420 
INLVPSNLPH EATRDTVFRG YVIPKGTVVI PTLDSLLFDN YEFPDPETFK PEHFLNENGK 

       430        440        450        460        470        480 
FKYSDYFKAF SAGKRVCVGE GLARMELFLL LSAILQHFNL KSLVDPKDID LSPVTIGFGS 

       490 
IPREFKLCVI PRS 

« Hide

References

« Hide 'large scale' references
[1]"Mouse ethanol-inducible cytochrome P-450 (P450IIE1). Characterization of cDNA clones and testosterone induction in kidney tissue."
Davis J.F., Felder M.R.
J. Biol. Chem. 268:16584-16589(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6S.
Tissue: Liver.
[2]"cDNA sequence, deduced amino acid sequence, predicted gene structure and chemical regulation of mouse Cyp2e1."
Freeman J.E., Stirling D., Russel A.L., Wolf C.R.
Biochem. J. 281:689-695(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[4]"Primary structure of the repeating element B2 and of the adjoining sequences in cloned mRNA actively transcribing in mouse liver cells."
Ivanov P.L., Ryskov A.P., Kramerov D.A., Georgiev G.P.
Dokl. Akad. Nauk SSSR 269:227-230(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
Tissue: Liver.
[5]"Mouse ubiquitous B2 repeat in polysomal and cytoplasmic poly(A)+RNAs: unidirectional orientation and 3'-end localization."
Ryskov A.P., Ivanov P.L., Kramerov D.A., Georgiev G.P.
Nucleic Acids Res. 11:6541-6558(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
[6]"Universal orientation and 3' terminal localization of repetitive sequences of the B2 family in mRNA."
Ryskov A.P., Ivanov P.L., Kramerov D.A., Georgiev G.P.
Mol. Biol. (Mosk.) 18:74-83(1984)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 455-493.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11650 mRNA. Translation: AAA39879.1.
X62595 mRNA. Translation: CAA44481.1.
BC013451 mRNA. Translation: AAH13451.1.
M54877 mRNA. Translation: AAA37275.1.
X01026 mRNA. Translation: CAA25510.1. Sequence problems.
PIRA47350.
A21231. S19657.
RefSeqNP_067257.1. NM_021282.2.
UniGeneMm.21758.

3D structure databases

ProteinModelPortalQ05421.
SMRQ05421. Positions 32-493.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ05421. 2 interactions.
MINTMINT-1862880.

PTM databases

PhosphoSiteQ05421.

Proteomic databases

PaxDbQ05421.
PRIDEQ05421.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026552; ENSMUSP00000026552; ENSMUSG00000025479.
GeneID13106.
KEGGmmu:13106.
UCSCuc009kic.1. mouse.

Organism-specific databases

CTD1571.
MGIMGI:88607. Cyp2e1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00680000099783.
HOGENOMHOG000036992.
HOVERGENHBG015789.
InParanoidQ05421.
KOK07415.
OMAPMYTMEN.
OrthoDBEOG7RBZ85.
PhylomeDBQ05421.
TreeFamTF352043.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12920.

Gene expression databases

BgeeQ05421.
GenevestigatorQ05421.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01687. EP450ICYP2E.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCYP2E1. mouse.
NextBio283102.
PROQ05421.
SOURCESearch...

Entry information

Entry nameCP2E1_MOUSE
AccessionPrimary (citable) accession number: Q05421
Secondary accession number(s): Q9Z198
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot