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Q053V3

- SYI_LEPBL

UniProt

Q053V3 - SYI_LEPBL

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain L550)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei557 – 5571Aminoacyl-adenylateUniRule annotation
Binding sitei601 – 6011ATPUniRule annotation
Metal bindingi889 – 8891ZincUniRule annotation
Metal bindingi892 – 8921ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLBOR355276:GHUQ-833-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LBL_0837
OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain L550)
Taxonomic identifieri355276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000000655: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Isoleucine--tRNA ligasePRO_1000022089Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi355276.LBL_0837.

Structurei

3D structure databases

ProteinModelPortaliQ053V3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionAdd
BLAST
Motifi598 – 6025"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q053V3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSETQKENPY SSTVLLPKTD FPMKADLAKR EPEQIRSWKQ NRIFRKMREQ
60 70 80 90 100
RSGKKEFVLH DGPPYANGNF HLGHALNKIL KDTIIKSKSL AGFYADMIPG
110 120 130 140 150
WDCHGLPIEV QVLKNLGKKV RETGPEELRQ LCRKYAEEFV GKQGDDLSRF
160 170 180 190 200
LCFWDEGRIY KTMSPDFEAK IVEVFGELFK KGYVYRGKKP VYWSIDLATA
210 220 230 240 250
HAEAEIEYYP HISPSIYVKF PIIGEKKRFC LIWTTTPWTL PANLAICFNR
260 270 280 290 300
KIEYSIFKTE SSEELILADA LAENVTITTG VALTKLKPIT SEELAALKFQ
310 320 330 340 350
HPFVDRISVS LFGDHVTLEA GTGCVHTAPG HGQDDYKVGL AAGLEPFSPV
360 370 380 390 400
DDYGRYTDEF PLMQGKKVFD ANPEIIQLLR DKGLLLYHGE LEHSYPHSWR
410 420 430 440 450
SKKPLIFRAT PQWFFKIDFQ DLREKSLSAI DGVRWIPSWG ITRIRSMVET
460 470 480 490 500
RPDWCLSRQR NWGVPIPAFT CESCGQTHID DASIQFFTKM VREKGIEIWY
510 520 530 540 550
SEETKDLLPP KTKCGKCGND SFKKGNDILD VWFDSGVSNF SVLGERKDEP
560 570 580 590 600
PADLYLEGSD QHRGWFQSSL WPSMALRGIP PYKAVLTHGY VLDEKGRAMS
610 620 630 640 650
KSLGNGIDPT ADIIQVYGAD ILRLWVSSLD FRDDIKVGKE SLKIVSEQYR
660 670 680 690 700
KIRNTFRYLL GNLDGHTPEQ NLPFEELEEL DRFYLSKLAG FVEDAVASYE
710 720 730 740 750
TYQFHQIYQK LILFCTVTLS QDYFDMIRDR MYCDLRDSKS RRSSSTALQY
760 770 780 790 800
ILDSLCILVA PILSFTAEEV WTSNGKKDSV FLQTFPDLKS WKNQSLEDKF
810 820 830 840 850
ESALQAREVV QKALEIARQE GKLGKSLEAA LEIVSKSGLS FGELLPKETL
860 870 880 890 900
ELLFVVSQIH EENPGMEVLS SHENEKFSVK VLKPLQGECP RCWRHTEDIS
910
KEGDLCGRCK SVVA
Length:914
Mass (Da):104,458
Last modified:November 14, 2006 - v1
Checksum:i32F8806EE48D446C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000348 Genomic DNA. Translation: ABJ78392.1.
RefSeqiYP_797325.1. NC_008508.1.

Genome annotation databases

EnsemblBacteriaiABJ78392; ABJ78392; LBL_0837.
GeneIDi4408799.
KEGGilbl:LBL_0837.
PATRICi22365188. VBILepBor75619_1058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000348 Genomic DNA. Translation: ABJ78392.1 .
RefSeqi YP_797325.1. NC_008508.1.

3D structure databases

ProteinModelPortali Q053V3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 355276.LBL_0837.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ78392 ; ABJ78392 ; LBL_0837 .
GeneIDi 4408799.
KEGGi lbl:LBL_0837.
PATRICi 22365188. VBILepBor75619_1058.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LBOR355276:GHUQ-833-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L550.

Entry informationi

Entry nameiSYI_LEPBL
AccessioniPrimary (citable) accession number: Q053V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3