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Q053V3

- SYI_LEPBL

UniProt

Q053V3 - SYI_LEPBL

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Leptospira borgpetersenii serovar Hardjo-bovis (strain L550)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei557 – 5571Aminoacyl-adenylateUniRule annotation
    Binding sitei601 – 6011ATPUniRule annotation
    Metal bindingi889 – 8891ZincUniRule annotation
    Metal bindingi892 – 8921ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLBOR355276:GHUQ-833-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LBL_0837
    OrganismiLeptospira borgpetersenii serovar Hardjo-bovis (strain L550)
    Taxonomic identifieri355276 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
    ProteomesiUP000000655: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 914914Isoleucine--tRNA ligasePRO_1000022089Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi355276.LBL_0837.

    Structurei

    3D structure databases

    ProteinModelPortaliQ053V3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionAdd
    BLAST
    Motifi598 – 6025"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q053V3-1 [UniParc]FASTAAdd to Basket

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    MSETQKENPY SSTVLLPKTD FPMKADLAKR EPEQIRSWKQ NRIFRKMREQ    50
    RSGKKEFVLH DGPPYANGNF HLGHALNKIL KDTIIKSKSL AGFYADMIPG 100
    WDCHGLPIEV QVLKNLGKKV RETGPEELRQ LCRKYAEEFV GKQGDDLSRF 150
    LCFWDEGRIY KTMSPDFEAK IVEVFGELFK KGYVYRGKKP VYWSIDLATA 200
    HAEAEIEYYP HISPSIYVKF PIIGEKKRFC LIWTTTPWTL PANLAICFNR 250
    KIEYSIFKTE SSEELILADA LAENVTITTG VALTKLKPIT SEELAALKFQ 300
    HPFVDRISVS LFGDHVTLEA GTGCVHTAPG HGQDDYKVGL AAGLEPFSPV 350
    DDYGRYTDEF PLMQGKKVFD ANPEIIQLLR DKGLLLYHGE LEHSYPHSWR 400
    SKKPLIFRAT PQWFFKIDFQ DLREKSLSAI DGVRWIPSWG ITRIRSMVET 450
    RPDWCLSRQR NWGVPIPAFT CESCGQTHID DASIQFFTKM VREKGIEIWY 500
    SEETKDLLPP KTKCGKCGND SFKKGNDILD VWFDSGVSNF SVLGERKDEP 550
    PADLYLEGSD QHRGWFQSSL WPSMALRGIP PYKAVLTHGY VLDEKGRAMS 600
    KSLGNGIDPT ADIIQVYGAD ILRLWVSSLD FRDDIKVGKE SLKIVSEQYR 650
    KIRNTFRYLL GNLDGHTPEQ NLPFEELEEL DRFYLSKLAG FVEDAVASYE 700
    TYQFHQIYQK LILFCTVTLS QDYFDMIRDR MYCDLRDSKS RRSSSTALQY 750
    ILDSLCILVA PILSFTAEEV WTSNGKKDSV FLQTFPDLKS WKNQSLEDKF 800
    ESALQAREVV QKALEIARQE GKLGKSLEAA LEIVSKSGLS FGELLPKETL 850
    ELLFVVSQIH EENPGMEVLS SHENEKFSVK VLKPLQGECP RCWRHTEDIS 900
    KEGDLCGRCK SVVA 914
    Length:914
    Mass (Da):104,458
    Last modified:November 14, 2006 - v1
    Checksum:i32F8806EE48D446C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000348 Genomic DNA. Translation: ABJ78392.1.
    RefSeqiYP_797325.1. NC_008508.1.

    Genome annotation databases

    EnsemblBacteriaiABJ78392; ABJ78392; LBL_0837.
    GeneIDi4408799.
    KEGGilbl:LBL_0837.
    PATRICi22365188. VBILepBor75619_1058.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000348 Genomic DNA. Translation: ABJ78392.1 .
    RefSeqi YP_797325.1. NC_008508.1.

    3D structure databases

    ProteinModelPortali Q053V3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 355276.LBL_0837.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABJ78392 ; ABJ78392 ; LBL_0837 .
    GeneIDi 4408799.
    KEGGi lbl:LBL_0837.
    PATRICi 22365188. VBILepBor75619_1058.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LBOR355276:GHUQ-833-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: L550.

    Entry informationi

    Entry nameiSYI_LEPBL
    AccessioniPrimary (citable) accession number: Q053V3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3