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Q05354 (HPCD_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-carboxymethyl-2-hydroxymuconate Delta-isomerase

EC=5.3.3.10
Alternative name(s):
5-carboxymethyl-2-hydroxymuconic acid isomerase
Short name=CHM isomerase
Short name=CHMI
Gene names
Name:hpcD
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length126 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transforms 5-carboxymethyl-2-hydroxy-muconic acid (CHM) into 5-oxo-pent-3-ene-1,2,5-tricarboxylic acid (OPET).

Catalytic activity

5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate.

Pathway

Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 4/7.

Subunit structure

Homotrimer.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   Molecular functionIsomerase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_function5-carboxymethyl-2-hydroxymuconate delta-isomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 1261255-carboxymethyl-2-hydroxymuconate Delta-isomerase
PRO_0000084041

Sites

Active site21Proton acceptor; via imino nitrogen

Secondary structure

........................ 126
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q05354 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 7494E625A76BBCEC

FASTA12614,215
        10         20         30         40         50         60 
MPHFIVECSD NIREEADLPG LFAKVNPTLA ATGIFPLAGI RSRVHWVDTW QMADGQHDYA 

        70         80         90        100        110        120 
SVHMTLKIGA GRSLESRQQA GEMLFELIKT HFAALMESRL LALSFEIEEL HPTLNFKQNN 


VHALFK 

« Hide

References

[1]"Purification, some properties and nucleotide sequence of 5-carboxymethyl-2-hydroxymuconate isomerase of Escherichia coli C."
Roper D.I., Cooper R.A.
FEBS Lett. 266:63-66(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C.
[2]"Subcloning and nucleotide sequence of the 3,4-dihydroxyphenylacetate (homoprotocatechuate) 2,3-dioxygenase gene from Escherichia coli C."
Roper D.I., Cooper R.A.
FEBS Lett. 275:53-57(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
Strain: C.
[3]"Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases."
Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J., Wilson K.S., Wigley D.B.
Biochemistry 35:792-802(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53666 Genomic DNA. Translation: CAA37707.1.
X55200 Genomic DNA. Translation: CAA38986.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OTGX-ray2.10A/B/C2-126[»]
ProteinModelPortalQ05354.
SMRQ05354. Positions 2-126.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00208; UER00419.

Family and domain databases

InterProIPR004220. 5-COMe_2-OHmuconate_Isoase.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PfamPF02962. CHMI. 1 hit.
[Graphical view]
SUPFAMSSF55331. SSF55331. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ05354.

Entry information

Entry nameHPCD_ECOLX
AccessionPrimary (citable) accession number: Q05354
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 69 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways