Q05354 (HPCD_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-carboxymethyl-2-hydroxymuconate Delta-isomerase
EC=5.3.3.10

Alternative name(s):
5-carboxymethyl-2-hydroxymuconic acid isomerase
Short name=CHM isomerase
Short name=CHMI

Gene names

Name:

hpcD

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	126 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transforms 5-carboxymethyl-2-hydroxy-muconic acid (CHM) into 5-oxo-pent-3-ene-1,2,5-tricarboxylic acid (OPET).
Catalytic activity	5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate.
Pathway	Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 4/7.
Subunit structure	Homotrimer.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Isomerase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	5-carboxymethyl-2-hydroxymuconate delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 126

125

5-carboxymethyl-2-hydroxymuconate Delta-isomerase

PRO_0000084041

Sites

Active site

2

1

Proton acceptor; via imino nitrogen

Secondary structure

1

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126

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Helix

10 – 12

3

Helix

13 – 16

4

Helix

18 – 30

13

Beta strand

33 – 35

3

Helix

37 – 39

3

Beta strand

41 – 52

12

Beta strand

59 – 68

10

Helix

74 – 91

18

Helix

93 – 96

4

Beta strand

99 – 109

11

Beta strand

112 – 119

8

Helix

120 – 124

5

Sequences

	Sequence		Length	Mass (Da)
	Q05354 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7494E625A76BBCEC	FASTA	126	14,215
	10 20 30 40 50 60 MPHFIVECSD NIREEADLPG LFAKVNPTLA ATGIFPLAGI RSRVHWVDTW QMADGQHDYA 70 80 90 100 110 120 SVHMTLKIGA GRSLESRQQA GEMLFELIKT HFAALMESRL LALSFEIEEL HPTLNFKQNN VHALFK

References

[1]	"Purification, some properties and nucleotide sequence of 5-carboxymethyl-2-hydroxymuconate isomerase of Escherichia coli C." Roper D.I., Cooper R.A. FEBS Lett. 266:63-66(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C.
[2]	"Subcloning and nucleotide sequence of the 3,4-dihydroxyphenylacetate (homoprotocatechuate) 2,3-dioxygenase gene from Escherichia coli C." Roper D.I., Cooper R.A. FEBS Lett. 275:53-57(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6. Strain: C.
[3]	"Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases." Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J., Wilson K.S., Wigley D.B. Biochemistry 35:792-802(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X53666 Genomic DNA. Translation: CAA37707.1.
X55200 Genomic DNA. Translation: CAA38986.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OTG	X-ray	2.10	A/B/C	2-126	[»]

ProteinModelPortal

Q05354.

SMR

Q05354. Positions 2-126.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

UniPathway

UPA00208; UER00419.

Family and domain databases

InterPro

IPR004220. 5-COMe_2-OHmuconate_Isoase.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]

Pfam

PF02962. CHMI. 1 hit.
[Graphical view]

SUPFAM

SSF55331. SSF55331. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q05354.

Entry information

Entry name

HPCD_ECOLX

Accession

Primary (citable) accession number: Q05354

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program