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Q05354

- HPCD_ECOLX

UniProt

Q05354 - HPCD_ECOLX

Protein

5-carboxymethyl-2-hydroxymuconate Delta-isomerase

Gene

hpcD

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Transforms 5-carboxymethyl-2-hydroxy-muconic acid (CHM) into 5-oxo-pent-3-ene-1,2,5-tricarboxylic acid (OPET).

    Catalytic activityi

    5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Proton acceptor; via imino nitrogen

    GO - Molecular functioni

    1. 5-carboxymethyl-2-hydroxymuconate delta-isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Enzyme and pathway databases

    UniPathwayiUPA00208; UER00419.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-carboxymethyl-2-hydroxymuconate Delta-isomerase (EC:5.3.3.10)
    Alternative name(s):
    5-carboxymethyl-2-hydroxymuconic acid isomerase
    Short name:
    CHM isomerase
    Short name:
    CHMI
    Gene namesi
    Name:hpcD
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 1261255-carboxymethyl-2-hydroxymuconate Delta-isomerasePRO_0000084041Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.

    Structurei

    Secondary structure

    1
    126
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 97
    Helixi10 – 123
    Helixi13 – 164
    Helixi18 – 3013
    Beta strandi33 – 353
    Helixi37 – 393
    Beta strandi41 – 5212
    Beta strandi59 – 6810
    Helixi74 – 9118
    Helixi93 – 964
    Beta strandi99 – 10911
    Beta strandi112 – 1198
    Helixi120 – 1245

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OTGX-ray2.10A/B/C2-126[»]
    ProteinModelPortaliQ05354.
    SMRiQ05354. Positions 2-126.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ05354.

    Family & Domainsi

    Family and domain databases

    InterProiIPR004220. 5-COMe_2-OHmuconate_Isoase.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view]
    PfamiPF02962. CHMI. 1 hit.
    [Graphical view]
    SUPFAMiSSF55331. SSF55331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05354-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPHFIVECSD NIREEADLPG LFAKVNPTLA ATGIFPLAGI RSRVHWVDTW    50
    QMADGQHDYA SVHMTLKIGA GRSLESRQQA GEMLFELIKT HFAALMESRL 100
    LALSFEIEEL HPTLNFKQNN VHALFK 126
    Length:126
    Mass (Da):14,215
    Last modified:January 23, 2007 - v3
    Checksum:i7494E625A76BBCEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53666 Genomic DNA. Translation: CAA37707.1.
    X55200 Genomic DNA. Translation: CAA38986.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53666 Genomic DNA. Translation: CAA37707.1 .
    X55200 Genomic DNA. Translation: CAA38986.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OTG X-ray 2.10 A/B/C 2-126 [» ]
    ProteinModelPortali Q05354.
    SMRi Q05354. Positions 2-126.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00208 ; UER00419 .

    Miscellaneous databases

    EvolutionaryTracei Q05354.

    Family and domain databases

    InterProi IPR004220. 5-COMe_2-OHmuconate_Isoase.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view ]
    Pfami PF02962. CHMI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55331. SSF55331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification, some properties and nucleotide sequence of 5-carboxymethyl-2-hydroxymuconate isomerase of Escherichia coli C."
      Roper D.I., Cooper R.A.
      FEBS Lett. 266:63-66(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C.
    2. "Subcloning and nucleotide sequence of the 3,4-dihydroxyphenylacetate (homoprotocatechuate) 2,3-dioxygenase gene from Escherichia coli C."
      Roper D.I., Cooper R.A.
      FEBS Lett. 275:53-57(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
      Strain: C.
    3. "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases."
      Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J., Wilson K.S., Wigley D.B.
      Biochemistry 35:792-802(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

    Entry informationi

    Entry nameiHPCD_ECOLX
    AccessioniPrimary (citable) accession number: Q05354
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 16, 2013
    This is version 69 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3