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Protein

FACT complex subunit Ssrp1

Gene

Ssrp

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Binds specifically to single-stranded DNA and RNA with highest affinity for nucleotides G and U. The FACT complex is required for expression of Hox genes.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi555 – 621HMG boxPROSITE-ProRule annotationAdd BLAST67

GO - Molecular functioni

  • chromo shadow domain binding Source: CAFA
  • nucleosomal DNA binding Source: FlyBase
  • nucleosome binding Source: FlyBase
  • protein heterodimerization activity Source: CAFA
  • protein self-association Source: FlyBase
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • DNA replication Source: UniProtKB-KW
  • negative regulation of protein phosphorylation Source: UniProtKB
  • ovarian follicle cell development Source: FlyBase
  • positive regulation of actin filament polymerization Source: UniProtKB
  • regulation of chromatin assembly or disassembly Source: FlyBase
  • regulation of DNA binding Source: FlyBase
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, RNA-binding
Biological processDNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-112382 Formation of RNA Pol II elongation complex
R-DME-674695 RNA Polymerase II Pre-transcription Events
R-DME-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-DME-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
FACT complex subunit Ssrp1
Alternative name(s):
Chorion-factor 5
Facilitates chromatin transcription complex subunit Ssrp1
Recombination signal sequence recognition protein
Single-strand recognition protein
dSSRP1
Gene namesi
Name:Ssrp
Synonyms:CF5, SSRP1
ORF Names:CG4817
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010278 Ssrp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000486041 – 723FACT complex subunit Ssrp1Add BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei443Phosphoserine2 Publications1
Modified residuei628Phosphoserine1 Publication1
Modified residuei664Phosphoserine1 Publication1
Modified residuei668Phosphoserine1 Publication1
Modified residuei669Phosphothreonine1 Publication1
Modified residuei670Phosphoserine1 Publication1
Modified residuei671Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ05344
PRIDEiQ05344

PTM databases

iPTMnetiQ05344

Expressioni

Tissue specificityi

Expressed at highest levels in nurse cells of the ovary.

Developmental stagei

Abundant throughout oogenesis and embryogenesis, decreases during larval stages and increases again in pupae.

Gene expression databases

BgeeiFBgn0010278
ExpressionAtlasiQ05344 differential
GenevisibleiQ05344 DM

Interactioni

Subunit structurei

Component of the FACT complex, a stable heterodimer of dre4/spt16 and Ssrp. Interacts with CHD1 and TRL/GAGA.2 Publications

GO - Molecular functioni

  • chromo shadow domain binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • protein self-association Source: FlyBase

Protein-protein interaction databases

BioGridi63363, 10 interactors
IntActiQ05344, 2 interactors
STRINGi7227.FBpp0072151

Structurei

Secondary structure

1723
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi561 – 576Combined sources16
Helixi582 – 594Combined sources13
Helixi599 – 614Combined sources16
Turni615 – 617Combined sources3
Helixi618 – 620Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXLNMR-A555-624[»]
DisProtiDP00720
ProteinModelPortaliQ05344
SMRiQ05344
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05344

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi437 – 516Asp-rich (acidic)Add BLAST80
Compositional biasi519 – 698Lys-rich (basic)Add BLAST180

Sequence similaritiesi

Belongs to the SSRP1 family.Curated

Phylogenomic databases

eggNOGiKOG0526 Eukaryota
COG5165 LUCA
GeneTreeiENSGT00560000076898
InParanoidiQ05344
KOiK09272
OMAiTGDAICI
OrthoDBiEOG091G04JP
PhylomeDBiQ05344

Family and domain databases

Gene3Di1.10.30.10, 1 hit
2.30.29.220, 1 hit
2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR013719 DUF1747
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR011993 PH-like_dom_sf
IPR035417 POB3_N
IPR000969 SSrcognition
IPR024954 SSRP1_dom
IPR038167 SSRP1_sf
PfamiView protein in Pfam
PF00505 HMG_box, 1 hit
PF17292 POB3_N, 1 hit
PF08512 Rtt106, 1 hit
PF03531 SSrecog, 1 hit
PRINTSiPR00887 SSRCOGNITION
SMARTiView protein in SMART
SM00398 HMG, 1 hit
SM01287 Rtt106, 1 hit
SUPFAMiSSF47095 SSF47095, 1 hit
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q05344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSLEYNDI NAEVRGVLCS GRLKMTEQNI IFKNTKTGKV EQISAEDIDL
60 70 80 90 100
INSQKFVGTW GLRVFTKGGV LHRFTGFRDS EHEKLGKFIK AAYSQEMVEK
110 120 130 140 150
EMCVKGWNWG TARFMGSVLS FDKESKTIFE VPLSHVSQCV TGKNEVTLEF
160 170 180 190 200
HQNDDAPVGL LEMRFHIPAV ESAEEDPVDK FHQNVMSKAS VISASGESIA
210 220 230 240 250
IFREIQILTP RGRYDIKIFS TFFQLHGKTF DYKIPMDSVL RLFMLPHKDS
260 270 280 290 300
RQMFFVLSLD PPIKQGQTRY HYLVLLFAPD EETTIELPFS EAELRDKYEG
310 320 330 340 350
KLEKEISGPV YEVMGKVMKV LIGRKITGPG NFIGHSGTAA VGCSFKAAAG
360 370 380 390 400
YLYPLERGFI YIHKPPLHIR FEEISSVNFA RSGGSTRSFD FEVTLKNGTV
410 420 430 440 450
HIFSSIEKEE YAKLFDYITQ KKLHVSNMGK DKSGYKDVDF GDSDNENEPD
460 470 480 490 500
AYLARLKAEA REKEEDDDDG DSDEESTDED FKPNENESDV AEEYDSNVES
510 520 530 540 550
DSDDDSDASG GGGDSDGAKK KKEKKSEKKE KKEKKHKEKE RTKKPSKKKK
560 570 580 590 600
DSGKPKRATT AFMLWLNDTR ESIKRENPGI KVTEIAKKGG EMWKELKDKS
610 620 630 640 650
KWEDAAAKDK QRYHDEMRNY KPEAGGDSDN EKGGKSSKKR KTEPSPSKKA
660 670 680 690 700
NTSGSGFKSK EYISDDDSTS SDDEKDNEPA KKKSKPPSDG DAKKKKAKSE
710 720
SEPEESEEDS NASDEDEEDE ASD
Length:723
Mass (Da):81,533
Last modified:December 1, 2000 - v2
Checksum:iDE8017F75C6CA207
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13E → Q in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti33K → E in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti212G → R in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti428M → T in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti498V → E in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti504D → E in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti573I → Y in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti681K → T in AAO45187 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08825 mRNA Translation: AAA28914.1
X68408 mRNA Translation: CAA48471.1
AE013599 Genomic DNA Translation: AAF47064.1
BT004831 mRNA Translation: AAO45187.1
PIRiA48217
S33688
RefSeqiNP_523830.2, NM_079106.3
UniGeneiDm.3383

Genome annotation databases

EnsemblMetazoaiFBtr0072242; FBpp0072151; FBgn0010278
GeneIDi37767
KEGGidme:Dmel_CG4817

Similar proteinsi

Entry informationi

Entry nameiSSRP1_DROME
AccessioniPrimary (citable) accession number: Q05344
Secondary accession number(s): Q86NM5, Q9W1J4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health