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Protein

FACT complex subunit Ssrp1

Gene

Ssrp

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Binds specifically to single-stranded DNA and RNA with highest affinity for nucleotides G and U. The FACT complex is required for expression of Hox genes.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi555 – 621HMG boxPROSITE-ProRule annotationAdd BLAST67

GO - Molecular functioni

  • nucleosomal DNA binding Source: FlyBase
  • nucleosome binding Source: FlyBase
  • protein self-association Source: FlyBase
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • DNA replication Source: UniProtKB-KW
  • neurogenesis Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • regulation of chromatin assembly or disassembly Source: FlyBase
  • regulation of DNA binding Source: FlyBase
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
FACT complex subunit Ssrp1
Alternative name(s):
Chorion-factor 5
Facilitates chromatin transcription complex subunit Ssrp1
Recombination signal sequence recognition protein
Single-strand recognition protein
dSSRP1
Gene namesi
Name:Ssrp
Synonyms:CF5, SSRP1
ORF Names:CG4817
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010278. Ssrp.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: UniProtKB-SubCell
  • FACT complex Source: FlyBase
  • nucleolus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000486041 – 723FACT complex subunit Ssrp1Add BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei443Phosphoserine2 Publications1
Modified residuei628Phosphoserine1 Publication1
Modified residuei664Phosphoserine1 Publication1
Modified residuei668Phosphoserine1 Publication1
Modified residuei669Phosphothreonine1 Publication1
Modified residuei670Phosphoserine1 Publication1
Modified residuei671Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ05344.
PRIDEiQ05344.

PTM databases

iPTMnetiQ05344.

Expressioni

Tissue specificityi

Expressed at highest levels in nurse cells of the ovary.

Developmental stagei

Abundant throughout oogenesis and embryogenesis, decreases during larval stages and increases again in pupae.

Gene expression databases

BgeeiFBgn0010278.
ExpressionAtlasiQ05344. baseline.
GenevisibleiQ05344. DM.

Interactioni

Subunit structurei

Component of the FACT complex, a stable heterodimer of dre4/spt16 and Ssrp. Interacts with CHD1 and TRL/GAGA.2 Publications

GO - Molecular functioni

  • protein self-association Source: FlyBase

Protein-protein interaction databases

BioGridi63363. 7 interactors.
IntActiQ05344. 1 interactor.
STRINGi7227.FBpp0072151.

Structurei

Secondary structure

1723
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi561 – 576Combined sources16
Helixi582 – 594Combined sources13
Helixi599 – 614Combined sources16
Turni615 – 617Combined sources3
Helixi618 – 620Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXLNMR-A555-624[»]
DisProtiDP00720.
ProteinModelPortaliQ05344.
SMRiQ05344.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05344.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi437 – 516Asp-rich (acidic)Add BLAST80
Compositional biasi519 – 698Lys-rich (basic)Add BLAST180

Sequence similaritiesi

Belongs to the SSRP1 family.Curated
Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0526. Eukaryota.
COG5165. LUCA.
GeneTreeiENSGT00560000076898.
InParanoidiQ05344.
KOiK09272.
OMAiHEVFTTV.
OrthoDBiEOG091G04JP.
PhylomeDBiQ05344.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR013719. DUF1747.
IPR009071. HMG_box_dom.
IPR011993. PH_dom-like.
IPR000969. SSrcognition.
IPR024954. SSRP1_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF08512. Rtt106. 1 hit.
PF03531. SSrecog. 1 hit.
[Graphical view]
PRINTSiPR00887. SSRCOGNITION.
SMARTiSM00398. HMG. 1 hit.
SM01287. Rtt106. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSLEYNDI NAEVRGVLCS GRLKMTEQNI IFKNTKTGKV EQISAEDIDL
60 70 80 90 100
INSQKFVGTW GLRVFTKGGV LHRFTGFRDS EHEKLGKFIK AAYSQEMVEK
110 120 130 140 150
EMCVKGWNWG TARFMGSVLS FDKESKTIFE VPLSHVSQCV TGKNEVTLEF
160 170 180 190 200
HQNDDAPVGL LEMRFHIPAV ESAEEDPVDK FHQNVMSKAS VISASGESIA
210 220 230 240 250
IFREIQILTP RGRYDIKIFS TFFQLHGKTF DYKIPMDSVL RLFMLPHKDS
260 270 280 290 300
RQMFFVLSLD PPIKQGQTRY HYLVLLFAPD EETTIELPFS EAELRDKYEG
310 320 330 340 350
KLEKEISGPV YEVMGKVMKV LIGRKITGPG NFIGHSGTAA VGCSFKAAAG
360 370 380 390 400
YLYPLERGFI YIHKPPLHIR FEEISSVNFA RSGGSTRSFD FEVTLKNGTV
410 420 430 440 450
HIFSSIEKEE YAKLFDYITQ KKLHVSNMGK DKSGYKDVDF GDSDNENEPD
460 470 480 490 500
AYLARLKAEA REKEEDDDDG DSDEESTDED FKPNENESDV AEEYDSNVES
510 520 530 540 550
DSDDDSDASG GGGDSDGAKK KKEKKSEKKE KKEKKHKEKE RTKKPSKKKK
560 570 580 590 600
DSGKPKRATT AFMLWLNDTR ESIKRENPGI KVTEIAKKGG EMWKELKDKS
610 620 630 640 650
KWEDAAAKDK QRYHDEMRNY KPEAGGDSDN EKGGKSSKKR KTEPSPSKKA
660 670 680 690 700
NTSGSGFKSK EYISDDDSTS SDDEKDNEPA KKKSKPPSDG DAKKKKAKSE
710 720
SEPEESEEDS NASDEDEEDE ASD
Length:723
Mass (Da):81,533
Last modified:December 1, 2000 - v2
Checksum:iDE8017F75C6CA207
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13E → Q in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti33K → E in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti212G → R in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti428M → T in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti498V → E in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti504D → E in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti573I → Y in AAA28914 (PubMed:7688122).Curated1
Sequence conflicti681K → T in AAO45187 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08825 mRNA. Translation: AAA28914.1.
X68408 mRNA. Translation: CAA48471.1.
AE013599 Genomic DNA. Translation: AAF47064.1.
BT004831 mRNA. Translation: AAO45187.1.
PIRiA48217.
S33688.
RefSeqiNP_523830.2. NM_079106.3.
UniGeneiDm.3383.

Genome annotation databases

EnsemblMetazoaiFBtr0072242; FBpp0072151; FBgn0010278.
GeneIDi37767.
KEGGidme:Dmel_CG4817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08825 mRNA. Translation: AAA28914.1.
X68408 mRNA. Translation: CAA48471.1.
AE013599 Genomic DNA. Translation: AAF47064.1.
BT004831 mRNA. Translation: AAO45187.1.
PIRiA48217.
S33688.
RefSeqiNP_523830.2. NM_079106.3.
UniGeneiDm.3383.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXLNMR-A555-624[»]
DisProtiDP00720.
ProteinModelPortaliQ05344.
SMRiQ05344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63363. 7 interactors.
IntActiQ05344. 1 interactor.
STRINGi7227.FBpp0072151.

PTM databases

iPTMnetiQ05344.

Proteomic databases

PaxDbiQ05344.
PRIDEiQ05344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072242; FBpp0072151; FBgn0010278.
GeneIDi37767.
KEGGidme:Dmel_CG4817.

Organism-specific databases

CTDi37767.
FlyBaseiFBgn0010278. Ssrp.

Phylogenomic databases

eggNOGiKOG0526. Eukaryota.
COG5165. LUCA.
GeneTreeiENSGT00560000076898.
InParanoidiQ05344.
KOiK09272.
OMAiHEVFTTV.
OrthoDBiEOG091G04JP.
PhylomeDBiQ05344.

Miscellaneous databases

EvolutionaryTraceiQ05344.
GenomeRNAii37767.
PROiQ05344.

Gene expression databases

BgeeiFBgn0010278.
ExpressionAtlasiQ05344. baseline.
GenevisibleiQ05344. DM.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR013719. DUF1747.
IPR009071. HMG_box_dom.
IPR011993. PH_dom-like.
IPR000969. SSrcognition.
IPR024954. SSRP1_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF08512. Rtt106. 1 hit.
PF03531. SSrecog. 1 hit.
[Graphical view]
PRINTSiPR00887. SSRCOGNITION.
SMARTiSM00398. HMG. 1 hit.
SM01287. Rtt106. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSRP1_DROME
AccessioniPrimary (citable) accession number: Q05344
Secondary accession number(s): Q86NM5, Q9W1J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.