Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q05332 (GUNG_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase G

EC=3.2.1.4
Alternative name(s):
Cellulase G
Endo-1,4-beta-glucanase G
Short name=EgG
Gene names
Name:celG
Ordered Locus Names:Cthe_2872
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Domain

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcellulosome

Inferred from direct assay Ref.1. Source: MENGO

   Molecular_functioncellulase activity

Inferred from direct assay Ref.1. Source: MENGO

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030
Chain31 – 566536Endoglucanase G
PRO_0000007853

Regions

Repeat503 – 526241
Repeat536 – 549142
Region503 – 549472 X 24 AA approximate repeats

Sites

Active site2261Proton donor By similarity
Active site3811Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05332 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 2CC9DE1AD87C3178

FASTA56663,199
        10         20         30         40         50         60 
MKKAKAIFSL VVALMVLAIF CFAQNTGSTA TTAAAAVDSN NDDWLHCKGN KIYDMYGNEV 

        70         80         90        100        110        120 
WLTGANWFGF NCSENCFHGA WYDVKTILTS IADRGINLLR IPISTELLYS WMIGKPNPVS 

       130        140        150        160        170        180 
SVTASNNPPY HVVNPDFYDP ETDDVKNSME IFDIIMGYCK ELGIKVMIDI HSPDANNSGH 

       190        200        210        220        230        240 
NYELWYGKET STCGVVTTKM WIDTLVWLAD KYKNDDTIIA FDLKNEPHGK RGYTAEVPKL 

       250        260        270        280        290        300 
LAKWDNSTDE NNWKYAAETC AKAILEVNPK VLIVIEGVEQ YPKTEKGYTY DTPDIWGATG 

       310        320        330        340        350        360 
DASPWYSAWW GGNLRGVKDY PIDLGPLNSQ IVYSPHDYGP SVYAQPWFEK DFTMQTLLDD 

       370        380        390        400        410        420 
YWYDTWAYIH DQGIAPILIG EWGGHMDGGK NQKWMTLLRD YIVQNRIHHT FWCINPNSGD 

       430        440        450        460        470        480 
TGGLLGNDWS TWDEAKYALL KPALWQTKDG KFIGLDHKIP LGSKGISLGE YYGTPQASDP 

       490        500        510        520        530        540 
PATPTATPTK PAASSTPSFI YGDINSDGNV NSTDLGILKR IIVKNPPASA NMDAADVNAD 

       550        560 
GKVNSTDYTV LKRYLLRSID KLPHTT 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the celG gene of Clostridium thermocellum and characterization of its product, endoglucanase CelG."
Lemaire M., Beguin P.
J. Bacteriol. 175:3353-3360(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69390 Genomic DNA. Translation: CAA49187.1.
CP000568 Genomic DNA. Translation: ABN54070.1.
PIRA40589.
RefSeqYP_001039263.1. NC_009012.1.

3D structure databases

ProteinModelPortalQ05332.
SMRQ05332. Positions 498-565.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ05332. 1 interaction.
STRING203119.Cthe_2872.

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN54070; ABN54070; Cthe_2872.
GeneID4809152.
KEGGcth:Cthe_2872.
PATRIC19519842. VBICloThe47081_3073.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2730.
HOGENOMHOG000225207.
OMAKYKNDDT.
OrthoDBEOG6PS5R7.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-2979-MONOMER.
MetaCyc:MONOMER-16420.

Family and domain databases

Gene3D1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNG_CLOTH
AccessionPrimary (citable) accession number: Q05332
Secondary accession number(s): A3DJE1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries