Q05332 (GUNG_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase G
EC=3.2.1.4

Alternative name(s):
Cellulase G
Endo-1,4-beta-glucanase G
Short name=EgG

Gene names

Name:	celG
Ordered Locus Names:	Cthe_2872

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Domain	A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30
	Chain	31 – 566	536	Endoglucanase G		PRO_0000007853
Regions
	Repeat	503 – 526	24	1
	Repeat	536 – 549	14	2
	Region	503 – 549	47	2 X 24 AA approximate repeats
Sites
	Active site	226	1	Proton donor By similarity
	Active site	381	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05332 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 2CC9DE1AD87C3178	FASTA	566	63,199
	10 20 30 40 50 60 MKKAKAIFSL VVALMVLAIF CFAQNTGSTA TTAAAAVDSN NDDWLHCKGN KIYDMYGNEV 70 80 90 100 110 120 WLTGANWFGF NCSENCFHGA WYDVKTILTS IADRGINLLR IPISTELLYS WMIGKPNPVS 130 140 150 160 170 180 SVTASNNPPY HVVNPDFYDP ETDDVKNSME IFDIIMGYCK ELGIKVMIDI HSPDANNSGH 190 200 210 220 230 240 NYELWYGKET STCGVVTTKM WIDTLVWLAD KYKNDDTIIA FDLKNEPHGK RGYTAEVPKL 250 260 270 280 290 300 LAKWDNSTDE NNWKYAAETC AKAILEVNPK VLIVIEGVEQ YPKTEKGYTY DTPDIWGATG 310 320 330 340 350 360 DASPWYSAWW GGNLRGVKDY PIDLGPLNSQ IVYSPHDYGP SVYAQPWFEK DFTMQTLLDD 370 380 390 400 410 420 YWYDTWAYIH DQGIAPILIG EWGGHMDGGK NQKWMTLLRD YIVQNRIHHT FWCINPNSGD 430 440 450 460 470 480 TGGLLGNDWS TWDEAKYALL KPALWQTKDG KFIGLDHKIP LGSKGISLGE YYGTPQASDP 490 500 510 520 530 540 PATPTATPTK PAASSTPSFI YGDINSDGNV NSTDLGILKR IIVKNPPASA NMDAADVNAD 550 560 GKVNSTDYTV LKRYLLRSID KLPHTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the celG gene of Clostridium thermocellum and characterization of its product, endoglucanase CelG." Lemaire M., Beguin P. J. Bacteriol. 175:3353-3360(1993) [PubMed: 8501039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27405 / DSM 1237.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69390 Genomic DNA. Translation: CAA49187.1. CP000568 Genomic DNA. Translation: ABN54070.1.
PIR	A40589.
RefSeq	YP_001039263.1. NC_009012.1.
3D structure databases
ProteinModelPortal	Q05332.
SMR	Q05332. Positions 498-565.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q05332. 1 interaction.
STRING	Q05332.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4809152.
GenomeReviews	Gene locus Cthe_2872 in contig CP000568_GR.
KEGG	cth:Cthe_2872.
NMPDR	fig|203119.1.peg.1532.
PATRIC	19519842. VBICloThe47081_3073.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2730.
HOGENOM	HBG424651.
OMA	PDANNSG.
ProtClustDB	CLSK882628.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16420.
Family and domain databases
InterPro	IPR016134. Cellulos_enz_dockerin_1. IPR002105. Cellulos_enz_dockerin_1_Ca-bd. IPR018242. Dockerin_1. IPR018247. EF_Hand_1_Ca_BS. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:1.10.1330.10. Cellulos_enz_dockerin_1. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. PF00404. Dockerin_1. 2 hits. [Graphical view]
SUPFAM	SSF63446. Cellulos_enz_dockerin_1. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00448. CLOS_CELLULOSOME_RPT. 2 hits. PS00018. EF_HAND_1. 2 hits. Uncertain. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNG_CLOTH

Accession

Primary (citable) accession number: Q05332
Secondary accession number(s): A3DJE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program