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Q05332

- GUNG_CLOTH

UniProt

Q05332 - GUNG_CLOTH

Protein

Endoglucanase G

Gene

celG

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei226 – 2261Proton donorBy similarity
    Active sitei381 – 3811NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: MENGO

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-2979-MONOMER.
    MetaCyc:MONOMER-16420.

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase G (EC:3.2.1.4)
    Alternative name(s):
    Cellulase G
    Endo-1,4-beta-glucanase G
    Short name:
    EgG
    Gene namesi
    Name:celG
    Ordered Locus Names:Cthe_2872
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cellulosome Source: MENGO

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Add
    BLAST
    Chaini31 – 566536Endoglucanase GPRO_0000007853Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    IntActiQ05332. 1 interaction.
    STRINGi203119.Cthe_2872.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05332.
    SMRiQ05332. Positions 498-565.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati503 – 526241Add
    BLAST
    Repeati536 – 549142Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni503 – 549472 X 24 AA approximate repeatsAdd
    BLAST

    Domaini

    A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG2730.
    HOGENOMiHOG000225207.
    OMAiKYKNDDT.
    OrthoDBiEOG6PS5R7.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    PS00018. EF_HAND_1. 2 hits.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05332-1 [UniParc]FASTAAdd to Basket

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    MKKAKAIFSL VVALMVLAIF CFAQNTGSTA TTAAAAVDSN NDDWLHCKGN    50
    KIYDMYGNEV WLTGANWFGF NCSENCFHGA WYDVKTILTS IADRGINLLR 100
    IPISTELLYS WMIGKPNPVS SVTASNNPPY HVVNPDFYDP ETDDVKNSME 150
    IFDIIMGYCK ELGIKVMIDI HSPDANNSGH NYELWYGKET STCGVVTTKM 200
    WIDTLVWLAD KYKNDDTIIA FDLKNEPHGK RGYTAEVPKL LAKWDNSTDE 250
    NNWKYAAETC AKAILEVNPK VLIVIEGVEQ YPKTEKGYTY DTPDIWGATG 300
    DASPWYSAWW GGNLRGVKDY PIDLGPLNSQ IVYSPHDYGP SVYAQPWFEK 350
    DFTMQTLLDD YWYDTWAYIH DQGIAPILIG EWGGHMDGGK NQKWMTLLRD 400
    YIVQNRIHHT FWCINPNSGD TGGLLGNDWS TWDEAKYALL KPALWQTKDG 450
    KFIGLDHKIP LGSKGISLGE YYGTPQASDP PATPTATPTK PAASSTPSFI 500
    YGDINSDGNV NSTDLGILKR IIVKNPPASA NMDAADVNAD GKVNSTDYTV 550
    LKRYLLRSID KLPHTT 566
    Length:566
    Mass (Da):63,199
    Last modified:February 1, 1994 - v1
    Checksum:i2CC9DE1AD87C3178
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69390 Genomic DNA. Translation: CAA49187.1.
    CP000568 Genomic DNA. Translation: ABN54070.1.
    PIRiA40589.
    RefSeqiWP_003514548.1. NC_009012.1.
    YP_001039263.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN54070; ABN54070; Cthe_2872.
    GeneIDi4809152.
    KEGGicth:Cthe_2872.
    PATRICi19519842. VBICloThe47081_3073.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69390 Genomic DNA. Translation: CAA49187.1 .
    CP000568 Genomic DNA. Translation: ABN54070.1 .
    PIRi A40589.
    RefSeqi WP_003514548.1. NC_009012.1.
    YP_001039263.1. NC_009012.1.

    3D structure databases

    ProteinModelPortali Q05332.
    SMRi Q05332. Positions 498-565.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q05332. 1 interaction.
    STRINGi 203119.Cthe_2872.

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN54070 ; ABN54070 ; Cthe_2872 .
    GeneIDi 4809152.
    KEGGi cth:Cthe_2872.
    PATRICi 19519842. VBICloThe47081_3073.

    Phylogenomic databases

    eggNOGi COG2730.
    HOGENOMi HOG000225207.
    OMAi KYKNDDT.
    OrthoDBi EOG6PS5R7.

    Enzyme and pathway databases

    BioCyci CTHE203119:GIW8-2979-MONOMER.
    MetaCyc:MONOMER-16420.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    PS00018. EF_HAND_1. 2 hits.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the celG gene of Clostridium thermocellum and characterization of its product, endoglucanase CelG."
      Lemaire M., Beguin P.
      J. Bacteriol. 175:3353-3360(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

    Entry informationi

    Entry nameiGUNG_CLOTH
    AccessioniPrimary (citable) accession number: Q05332
    Secondary accession number(s): A3DJE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3