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Protein

Minor nucleoprotein VP30

Gene

VP30

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 90C3H1-type; atypicalAdd BLAST19

GO - Molecular functioni

GO - Biological processi

  • positive regulation of viral transcription Source: CACAO
  • suppression of host defenses Source: CACAO
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene namesi
Name:VP30
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Proteomesi
  • UP000007209 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi100L → A: Complete loss of homo-oligomerization. 1 Publication1
Mutagenesisi102L → A: Complete loss of homo-oligomerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002221581 – 288Minor nucleoprotein VP30Add BLAST288

Post-translational modificationi

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer or homodimer. Interacts with the nucleoprotein (By similarity).By similarity

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi144 – 155Combined sources12
Helixi164 – 178Combined sources15
Helixi183 – 185Combined sources3
Helixi186 – 196Combined sources11
Helixi201 – 203Combined sources3
Helixi204 – 215Combined sources12
Helixi221 – 229Combined sources9
Helixi232 – 246Combined sources15
Helixi254 – 257Combined sources4
Turni258 – 260Combined sources3
Helixi261 – 264Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DVWX-ray1.75A/B/C/D142-272[»]
5T3TX-ray2.20A/B/C/D/E/F/G/H/I/J139-288[»]
DisProtiDP00627.
ProteinModelPortaliQ05323.
SMRiQ05323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 44Intrinsically disorderedSequence analysisAdd BLAST44
Regioni26 – 40RNA-bindingAdd BLAST15
Regioni94 – 112OligomerizationBy similarityAdd BLAST19
Regioni121 – 140Intrinsically disorderedSequence analysisAdd BLAST20
Regioni180 – 197Interaction with the nucleoproteinBy similarityAdd BLAST18
Regioni268 – 288Intrinsically disorderedSequence analysisAdd BLAST21

Sequence similaritiesi

Contains 1 C3H1-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 90C3H1-type; atypicalAdd BLAST19

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05323-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASYERGRP RAARQHSRDG HDHHVRARSS SRENYRGEYR QSRSASQVRV
60 70 80 90 100
PTVFHKKRVE PLTVPPAPKD ICPTLKKGFL CDSSFCKKDH QLESLTDREL
110 120 130 140 150
LLLIARKTCG SVEQQLNITA PKDSRLANPT ADDFQQEEGP KITLLTLIKT
160 170 180 190 200
AEHWARQDIR TIEDSKLRAL LTLCAVMTRK FSKSQLSLLC ETHLRREGLG
210 220 230 240 250
QDQAEPVLEV YQRLHSDKGG SFEAALWQQW DRQSLIMFIT AFLNIALQLP
260 270 280
CESSAVVVSG LRTLVPQSDN EEASTNPGTC SWSDEGTP
Length:288
Mass (Da):32,521
Last modified:May 30, 2000 - v2
Checksum:i1FE40E93AB80454B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256 – 288VVVSG…DEGTP → ALFQG in AAB81005 (PubMed:8237108).CuratedAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11365 Genomic RNA. Translation: AAB81005.1.
AF086833 Genomic RNA. Translation: AAD14587.1.
AF499101 Genomic RNA. Translation: AAM76036.1.
AY142960 Genomic RNA. Translation: AAN37509.1.
AF272001 Genomic RNA. Translation: AAG40169.1.
RefSeqiNP_066249.1. NC_002549.1.

Genome annotation databases

GeneIDi911826.
KEGGivg:911826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11365 Genomic RNA. Translation: AAB81005.1.
AF086833 Genomic RNA. Translation: AAD14587.1.
AF499101 Genomic RNA. Translation: AAM76036.1.
AY142960 Genomic RNA. Translation: AAN37509.1.
AF272001 Genomic RNA. Translation: AAG40169.1.
RefSeqiNP_066249.1. NC_002549.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DVWX-ray1.75A/B/C/D142-272[»]
5T3TX-ray2.20A/B/C/D/E/F/G/H/I/J139-288[»]
DisProtiDP00627.
ProteinModelPortaliQ05323.
SMRiQ05323.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi911826.
KEGGivg:911826.

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVP30_EBOZM
AccessioniPrimary (citable) accession number: Q05323
Secondary accession number(s): Q9YMG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: November 30, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.