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Q05323 (VP30_EBOZM) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene names
Name:VP30
OrganismZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) [Reference proteome]
Taxonomic identifier128952 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA. Ref.6

Subunit structure

Homohexamer or homodimer. Interacts with the nucleoprotein By similarity. Ref.7

Subcellular location

Virion. Host cytoplasm.

Post-translational modification

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation. Ref.5

Sequence similarities

Belongs to the filoviridae minor nucleoprotein VP30 family.

Contains 1 C3H1-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Minor nucleoprotein VP30
PRO_0000222158

Regions

Zinc finger72 – 9019C3H1-type; atypical
Region1 – 4444Intrinsically disordered Potential
Region26 – 4015RNA-binding
Region94 – 11219Oligomerization By similarity
Region121 – 14020Intrinsically disordered Potential
Region180 – 19718Interaction with the nucleoprotein By similarity
Region268 – 28821Intrinsically disordered Potential

Experimental info

Mutagenesis1001L → A: Complete loss of homo-oligomerization. Ref.9
Mutagenesis1021L → A: Complete loss of homo-oligomerization. Ref.9
Sequence conflict256 – 28833VVVSG…DEGTP → ALFQG in AAB81005. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q05323 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 1FE40E93AB80454B

FASTA28832,521
        10         20         30         40         50         60 
MEASYERGRP RAARQHSRDG HDHHVRARSS SRENYRGEYR QSRSASQVRV PTVFHKKRVE 

        70         80         90        100        110        120 
PLTVPPAPKD ICPTLKKGFL CDSSFCKKDH QLESLTDREL LLLIARKTCG SVEQQLNITA 

       130        140        150        160        170        180 
PKDSRLANPT ADDFQQEEGP KITLLTLIKT AEHWARQDIR TIEDSKLRAL LTLCAVMTRK 

       190        200        210        220        230        240 
FSKSQLSLLC ETHLRREGLG QDQAEPVLEV YQRLHSDKGG SFEAALWQQW DRQSLIMFIT 

       250        260        270        280 
AFLNIALQLP CESSAVVVSG LRTLVPQSDN EEASTNPGTC SWSDEGTP 

« Hide

References

[1]"Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Molecular characterization of guinea pig-adapted variants of Ebola virus."
Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate guinea pig-adapted.
[4]Ichou M.A., Paragas J., Jahrling P.B., Ibrahim M.S., Lofts L., Hevey M., Schmaljohn A.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[5]"Phosphorylation of VP30 impairs ebola virus transcription."
Modrof J., Muhlberger E., Klenk H.D., Becker S.
J. Biol. Chem. 277:33099-33104(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[6]"Ebola virus VP30-mediated transcription is regulated by RNA secondary structure formation."
Weik M., Modrof J., Klenk H.D., Becker S., Muhlberger E.
J. Virol. 76:8532-8539(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
Huang Y., Xu L., Sun Y., Nabel G.J.
Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH THE NUCLEOPROTEIN.
[8]"Ebola virus transcription activator VP30 is a zinc-binding protein."
Modrof J., Becker S., Muhlberger E.
J. Virol. 77:3334-3338(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ZINC-FINGER.
[9]"Oligomerization of Ebola virus VP30 is essential for viral transcription and can be inhibited by a synthetic peptide."
Hartlieb B., Modrof J., Muhlberger E., Klenk H.D., Becker S.
J. Biol. Chem. 278:41830-41836(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF LEU-100 AND LEU-102.
[10]"Ebola virus VP30 is an RNA binding protein."
John S.P., Wang T., Steffen S., Longhi S., Schmaljohn C.S., Jonsson C.B.
J. Virol. 81:8967-8976(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA-BINDING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11365 Genomic RNA. Translation: AAB81005.1.
AF086833 Genomic RNA. Translation: AAD14587.1.
AF499101 Genomic RNA. Translation: AAM76036.1.
AY142960 Genomic RNA. Translation: AAN37509.1.
AF272001 Genomic RNA. Translation: AAG40169.1.
RefSeqNP_066249.1. NC_002549.1.

3D structure databases

DisProtDP00627.
ProteinModelPortalQ05323.
SMRQ05323. Positions 142-266.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID911826.

Family and domain databases

InterProIPR014459. VP30_FiloV.
[Graphical view]
PfamPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFPIRSF011356. VP30_FiloV. 1 hit.
ProtoNetSearch...

Entry information

Entry nameVP30_EBOZM
AccessionPrimary (citable) accession number: Q05323
Secondary accession number(s): Q9YMG1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: March 19, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families