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Q05323

- VP30_EBOZM

UniProt

Q05323 - VP30_EBOZM

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Protein

Minor nucleoprotein VP30

Gene
VP30
Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 9019C3H1-type; atypicalAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of viral transcription Source: CACAO
  2. suppression of host defenses Source: CACAO
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene namesi
Name:VP30
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000007209: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. ribonucleoprotein complex Source: CACAO
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi100 – 1001L → A: Complete loss of homo-oligomerization. 1 Publication
Mutagenesisi102 – 1021L → A: Complete loss of homo-oligomerization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Minor nucleoprotein VP30PRO_0000222158Add
BLAST

Post-translational modificationi

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer or homodimer. Interacts with the nucleoprotein By similarity.1 Publication

Structurei

3D structure databases

DisProtiDP00627.
ProteinModelPortaliQ05323.
SMRiQ05323. Positions 142-266.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4444Intrinsically disordered Reviewed predictionAdd
BLAST
Regioni26 – 4015RNA-bindingAdd
BLAST
Regioni94 – 11219Oligomerization By similarityAdd
BLAST
Regioni121 – 14020Intrinsically disordered Reviewed predictionAdd
BLAST
Regioni180 – 19718Interaction with the nucleoprotein By similarityAdd
BLAST
Regioni268 – 28821Intrinsically disordered Reviewed predictionAdd
BLAST

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 9019C3H1-type; atypicalAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05323-1 [UniParc]FASTAAdd to Basket

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MEASYERGRP RAARQHSRDG HDHHVRARSS SRENYRGEYR QSRSASQVRV    50
PTVFHKKRVE PLTVPPAPKD ICPTLKKGFL CDSSFCKKDH QLESLTDREL 100
LLLIARKTCG SVEQQLNITA PKDSRLANPT ADDFQQEEGP KITLLTLIKT 150
AEHWARQDIR TIEDSKLRAL LTLCAVMTRK FSKSQLSLLC ETHLRREGLG 200
QDQAEPVLEV YQRLHSDKGG SFEAALWQQW DRQSLIMFIT AFLNIALQLP 250
CESSAVVVSG LRTLVPQSDN EEASTNPGTC SWSDEGTP 288
Length:288
Mass (Da):32,521
Last modified:May 30, 2000 - v2
Checksum:i1FE40E93AB80454B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti256 – 28833VVVSG…DEGTP → ALFQG in AAB81005. 1 PublicationAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11365 Genomic RNA. Translation: AAB81005.1.
AF086833 Genomic RNA. Translation: AAD14587.1.
AF499101 Genomic RNA. Translation: AAM76036.1.
AY142960 Genomic RNA. Translation: AAN37509.1.
AF272001 Genomic RNA. Translation: AAG40169.1.
RefSeqiNP_066249.1. NC_002549.1.

Genome annotation databases

GeneIDi911826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11365 Genomic RNA. Translation: AAB81005.1 .
AF086833 Genomic RNA. Translation: AAD14587.1 .
AF499101 Genomic RNA. Translation: AAM76036.1 .
AY142960 Genomic RNA. Translation: AAN37509.1 .
AF272001 Genomic RNA. Translation: AAG40169.1 .
RefSeqi NP_066249.1. NC_002549.1.

3D structure databases

DisProti DP00627.
ProteinModelPortali Q05323.
SMRi Q05323. Positions 142-266.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911826.

Family and domain databases

InterProi IPR014459. VP30_FiloV.
[Graphical view ]
Pfami PF11507. Transcript_VP30. 1 hit.
[Graphical view ]
PIRSFi PIRSF011356. VP30_FiloV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
    Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
    Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Molecular characterization of guinea pig-adapted variants of Ebola virus."
    Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
    Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate guinea pig-adapted.
  4. Ichou M.A., Paragas J., Jahrling P.B., Ibrahim M.S., Lofts L., Hevey M., Schmaljohn A.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  5. "Phosphorylation of VP30 impairs ebola virus transcription."
    Modrof J., Muhlberger E., Klenk H.D., Becker S.
    J. Biol. Chem. 277:33099-33104(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  6. "Ebola virus VP30-mediated transcription is regulated by RNA secondary structure formation."
    Weik M., Modrof J., Klenk H.D., Becker S., Muhlberger E.
    J. Virol. 76:8532-8539(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
    Huang Y., Xu L., Sun Y., Nabel G.J.
    Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THE NUCLEOPROTEIN.
  8. "Ebola virus transcription activator VP30 is a zinc-binding protein."
    Modrof J., Becker S., Muhlberger E.
    J. Virol. 77:3334-3338(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ZINC-FINGER.
  9. "Oligomerization of Ebola virus VP30 is essential for viral transcription and can be inhibited by a synthetic peptide."
    Hartlieb B., Modrof J., Muhlberger E., Klenk H.D., Becker S.
    J. Biol. Chem. 278:41830-41836(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LEU-100 AND LEU-102.
  10. Cited for: RNA-BINDING.

Entry informationi

Entry nameiVP30_EBOZM
AccessioniPrimary (citable) accession number: Q05323
Secondary accession number(s): Q9YMG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: March 19, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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