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Q05323

- VP30_EBOZM

UniProt

Q05323 - VP30_EBOZM

Protein

Minor nucleoprotein VP30

Gene

VP30

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri72 – 9019C3H1-type; atypicalAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. positive regulation of viral transcription Source: CACAO
    2. suppression of host defenses Source: CACAO
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Minor nucleoprotein VP30
    Alternative name(s):
    Transcription activator VP30
    Gene namesi
    Name:VP30
    OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
    Taxonomic identifieri128952 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    ProteomesiUP000007209: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. ribonucleoprotein complex Source: CACAO
    3. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi100 – 1001L → A: Complete loss of homo-oligomerization. 1 Publication
    Mutagenesisi102 – 1021L → A: Complete loss of homo-oligomerization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Minor nucleoprotein VP30PRO_0000222158Add
    BLAST

    Post-translational modificationi

    Phosphorylated by host. Phosphorylation negatively regulates the transcription activation.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homohexamer or homodimer. Interacts with the nucleoprotein By similarity.By similarity

    Structurei

    3D structure databases

    DisProtiDP00627.
    ProteinModelPortaliQ05323.
    SMRiQ05323. Positions 142-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4444Intrinsically disorderedSequence AnalysisAdd
    BLAST
    Regioni26 – 4015RNA-bindingAdd
    BLAST
    Regioni94 – 11219OligomerizationBy similarityAdd
    BLAST
    Regioni121 – 14020Intrinsically disorderedSequence AnalysisAdd
    BLAST
    Regioni180 – 19718Interaction with the nucleoproteinBy similarityAdd
    BLAST
    Regioni268 – 28821Intrinsically disorderedSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C3H1-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri72 – 9019C3H1-type; atypicalAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR014459. VP30_FiloV.
    [Graphical view]
    PfamiPF11507. Transcript_VP30. 1 hit.
    [Graphical view]
    PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q05323-1 [UniParc]FASTAAdd to Basket

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    MEASYERGRP RAARQHSRDG HDHHVRARSS SRENYRGEYR QSRSASQVRV    50
    PTVFHKKRVE PLTVPPAPKD ICPTLKKGFL CDSSFCKKDH QLESLTDREL 100
    LLLIARKTCG SVEQQLNITA PKDSRLANPT ADDFQQEEGP KITLLTLIKT 150
    AEHWARQDIR TIEDSKLRAL LTLCAVMTRK FSKSQLSLLC ETHLRREGLG 200
    QDQAEPVLEV YQRLHSDKGG SFEAALWQQW DRQSLIMFIT AFLNIALQLP 250
    CESSAVVVSG LRTLVPQSDN EEASTNPGTC SWSDEGTP 288
    Length:288
    Mass (Da):32,521
    Last modified:May 30, 2000 - v2
    Checksum:i1FE40E93AB80454B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti256 – 28833VVVSG…DEGTP → ALFQG in AAB81005. (PubMed:8237108)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11365 Genomic RNA. Translation: AAB81005.1.
    AF086833 Genomic RNA. Translation: AAD14587.1.
    AF499101 Genomic RNA. Translation: AAM76036.1.
    AY142960 Genomic RNA. Translation: AAN37509.1.
    AF272001 Genomic RNA. Translation: AAG40169.1.
    RefSeqiNP_066249.1. NC_002549.1.

    Genome annotation databases

    GeneIDi911826.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11365 Genomic RNA. Translation: AAB81005.1 .
    AF086833 Genomic RNA. Translation: AAD14587.1 .
    AF499101 Genomic RNA. Translation: AAM76036.1 .
    AY142960 Genomic RNA. Translation: AAN37509.1 .
    AF272001 Genomic RNA. Translation: AAG40169.1 .
    RefSeqi NP_066249.1. NC_002549.1.

    3D structure databases

    DisProti DP00627.
    ProteinModelPortali Q05323.
    SMRi Q05323. Positions 142-266.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 911826.

    Family and domain databases

    InterProi IPR014459. VP30_FiloV.
    [Graphical view ]
    Pfami PF11507. Transcript_VP30. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF011356. VP30_FiloV. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
      Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
      Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Molecular characterization of guinea pig-adapted variants of Ebola virus."
      Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
      Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate guinea pig-adapted.
    4. Ichou M.A., Paragas J., Jahrling P.B., Ibrahim M.S., Lofts L., Hevey M., Schmaljohn A.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    5. "Phosphorylation of VP30 impairs ebola virus transcription."
      Modrof J., Muhlberger E., Klenk H.D., Becker S.
      J. Biol. Chem. 277:33099-33104(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.
    6. "Ebola virus VP30-mediated transcription is regulated by RNA secondary structure formation."
      Weik M., Modrof J., Klenk H.D., Becker S., Muhlberger E.
      J. Virol. 76:8532-8539(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
      Huang Y., Xu L., Sun Y., Nabel G.J.
      Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE NUCLEOPROTEIN.
    8. "Ebola virus transcription activator VP30 is a zinc-binding protein."
      Modrof J., Becker S., Muhlberger E.
      J. Virol. 77:3334-3338(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ZINC-FINGER.
    9. "Oligomerization of Ebola virus VP30 is essential for viral transcription and can be inhibited by a synthetic peptide."
      Hartlieb B., Modrof J., Muhlberger E., Klenk H.D., Becker S.
      J. Biol. Chem. 278:41830-41836(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LEU-100 AND LEU-102.
    10. Cited for: RNA-BINDING.

    Entry informationi

    Entry nameiVP30_EBOZM
    AccessioniPrimary (citable) accession number: Q05323
    Secondary accession number(s): Q9YMG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3