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Q05322

- VP24_EBOZM

UniProt

Q05322 - VP24_EBOZM

Protein

Membrane-associated protein VP24

Gene

VP24

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. Blocks the IFN-induced nuclear accumulation of host phosphorylated STAT1, by interacting with the STAT1-binding region of host importin alpha-1/KPNA1 protein, thereby inhibiting the latter. Without the activity of this protein, activated STAT1 would not enter the nucleus and be unable to activate IFN-induced genes. Plays a role in assembly of viral nucleocapsid and virion budding. May act as a minor matrix protein that plays a role in assembly of viral nucleocapsid and virion budding.4 Publications

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. suppression by virus of host intracellular interferon activity Source: CACAO
    2. viral budding from plasma membrane Source: CACAO

    Keywords - Biological processi

    Host-virus interaction, Interferon antiviral system evasion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane-associated protein VP24
    Gene namesi
    Name:VP24
    OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
    Taxonomic identifieri128952 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    ProteomesiUP000007209: Genome

    Subcellular locationi

    Virion membrane 1 Publication; Peripheral membrane protein 1 Publication. Host cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Host endomembrane system 1 Publication; Peripheral membrane protein 1 Publication
    Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and to the plasma membrane where budding takes place By similarity.By similarity

    GO - Cellular componenti

    1. host cell endomembrane system Source: UniProtKB-SubCell
    2. host cell plasma membrane Source: CACAO
    3. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Membrane-associated protein VP24PRO_0000222154Add
    BLAST

    Interactioni

    Subunit structurei

    Binds to host importin alpha-1/KPNA1.

    Protein-protein interaction databases

    IntActiQ05322. 36 interactions.

    Structurei

    Secondary structure

    1
    251
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi16 – 2611
    Beta strandi30 – 334
    Beta strandi35 – 428
    Beta strandi45 – 506
    Helixi54 – 607
    Beta strandi62 – 654
    Turni67 – 693
    Helixi70 – 745
    Helixi77 – 804
    Beta strandi85 – 873
    Helixi90 – 10516
    Beta strandi108 – 1103
    Turni113 – 1153
    Helixi116 – 12813
    Helixi139 – 1424
    Helixi147 – 16519
    Beta strandi172 – 1743
    Beta strandi178 – 1825
    Beta strandi187 – 1937
    Beta strandi196 – 2027
    Helixi207 – 2093
    Beta strandi211 – 2133
    Beta strandi217 – 2226
    Helixi224 – 2285

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4M0QX-ray1.92A/B11-237[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR009433. Filo_VP24.
    [Graphical view]
    PfamiPF06389. Filo_VP24. 1 hit.
    [Graphical view]
    PIRSFiPIRSF011355. VP24. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q05322-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKATGRYNL ISPKKDLEKG VVLSDLCNFL VSQTIQGWKV YWAGIEFDVT    50
    HKGMALLHRL KTNDFAPAWS MTRNLFPHLF QNPNSTIESP LWALRVILAA 100
    GIQDQLIDQS LIEPLAGALG LISDWLLTTN TNHFNMRTQR VKEQLSLKML 150
    SLIRSNILKF INKLDALHVV NYNGLLSSIE IGTQNHTIII TRTNMGFLVE 200
    LQEPDKSAMN RMKPGPAKFS LLHESTLKAF TQGSSTRMQS LILEFNSSLA 250
    I 251
    Length:251
    Mass (Da):28,219
    Last modified:July 15, 1998 - v2
    Checksum:i5A8F356AF2CC5A5C
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti50 – 501T → I in strain: Isolate mouse-adapted.
    Natural varianti71 – 711M → I in strain: Isolate guinea pig-adapted.
    Natural varianti147 – 1471L → P in strain: Isolate guinea pig-adapted.
    Natural varianti187 – 1871T → I in strain: Isolate guinea pig-adapted.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11365 Genomic RNA. Translation: AAB81006.1.
    AF086833 Genomic RNA. Translation: AAD14588.1.
    AF272001 Genomic RNA. Translation: AAG40170.1.
    AY142960 Genomic RNA. Translation: AAN37510.1.
    AF499101 Genomic RNA. Translation: AAM76037.1.
    RefSeqiNP_066250.1. NC_002549.1.

    Genome annotation databases

    GeneIDi911828.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11365 Genomic RNA. Translation: AAB81006.1 .
    AF086833 Genomic RNA. Translation: AAD14588.1 .
    AF272001 Genomic RNA. Translation: AAG40170.1 .
    AY142960 Genomic RNA. Translation: AAN37510.1 .
    AF499101 Genomic RNA. Translation: AAM76037.1 .
    RefSeqi NP_066250.1. NC_002549.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4M0Q X-ray 1.92 A/B 11-237 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q05322. 36 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 911828.

    Family and domain databases

    InterProi IPR009433. Filo_VP24.
    [Graphical view ]
    Pfami PF06389. Filo_VP24. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF011355. VP24. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
      Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
      Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Characterization of the L gene and 5' trailer region of Ebola virus."
      Volchkov V.E., Volchkova V.A., Chepurnov A.A., Blinov V.M., Netesov S.V., Feldmann H.
      J. Gen. Virol. 80:355-362(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Molecular characterization of guinea pig-adapted variants of Ebola virus."
      Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
      Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate guinea pig-adapted.
    4. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate mouse-adapted.
    5. "The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
      Huang Y., Xu L., Sun Y., Nabel G.J.
      Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Biochemical and functional characterization of the Ebola virus VP24 protein: implications for a role in virus assembly and budding."
      Han Z., Boshra H., Sunyer J.O., Zwiers S.H., Paragas J., Harty R.N.
      J. Virol. 77:1793-1800(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles."
      Licata J.M., Johnson R.F., Han Z., Harty R.N.
      J. Virol. 78:7344-7351(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Ebola virus VP24 binds karyopherin alpha-1 and blocks STAT1 nuclear accumulation."
      Reid S.P., Leung L.W., Hartman A.L., Martinez O., Shaw M.L., Carbonnelle C., Volchkov V.E., Nichol S.T., Basler C.F.
      J. Virol. 80:5156-5167(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST KPNA1.
    9. "Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1."
      Reid S.P., Valmas C., Martinez O., Sanchez F.M., Basler C.F.
      J. Virol. 81:13469-13477(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOST KPNA1.

    Entry informationi

    Entry nameiVP24_EBOZM
    AccessioniPrimary (citable) accession number: Q05322
    Secondary accession number(s): Q773N1, Q8JS60, Q9DQD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3