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Q05322

- VP24_EBOZM

UniProt

Q05322 - VP24_EBOZM

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Protein

Membrane-associated protein VP24

Gene

VP24

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. Blocks the IFN-induced nuclear accumulation of host phosphorylated STAT1, by interacting with the STAT1-binding region of host importin alpha-1/KPNA1 protein, thereby inhibiting the latter. Without the activity of this protein, activated STAT1 would not enter the nucleus and be unable to activate IFN-induced genes. Plays a role in assembly of viral nucleocapsid and virion budding. May act as a minor matrix protein that plays a role in assembly of viral nucleocapsid and virion budding.4 Publications

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. suppression by virus of host intracellular interferon activity Source: CACAO
  2. viral budding from plasma membrane Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Interferon antiviral system evasion

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated protein VP24
Gene namesi
Name:VP24
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000007209: Genome

Subcellular locationi

Virion membrane 1 Publication; Peripheral membrane protein 1 Publication. Host cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Host endomembrane system 1 Publication; Peripheral membrane protein 1 Publication
Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and to the plasma membrane where budding takes place (By similarity).By similarity

GO - Cellular componenti

  1. host cell plasma membrane Source: CACAO
  2. membrane Source: UniProtKB-KW
  3. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Membrane-associated protein VP24PRO_0000222154Add
BLAST

Interactioni

Subunit structurei

Binds to host importin alpha-1/KPNA1.

Protein-protein interaction databases

IntActiQ05322. 36 interactions.

Structurei

Secondary structure

1
251
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 2611
Beta strandi30 – 334
Beta strandi35 – 428
Beta strandi45 – 506
Helixi54 – 607
Beta strandi62 – 654
Turni67 – 693
Helixi70 – 745
Helixi77 – 804
Beta strandi85 – 873
Helixi90 – 10516
Beta strandi108 – 1103
Turni113 – 1153
Helixi116 – 12813
Helixi139 – 1424
Helixi147 – 16519
Beta strandi172 – 1743
Beta strandi178 – 1825
Beta strandi187 – 1937
Beta strandi196 – 2027
Helixi207 – 2093
Beta strandi211 – 2133
Beta strandi217 – 2226
Helixi224 – 2285

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M0QX-ray1.92A/B11-237[»]
4U2XX-ray3.15A/B/C16-231[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR009433. Filo_VP24.
[Graphical view]
PfamiPF06389. Filo_VP24. 1 hit.
[Graphical view]
PIRSFiPIRSF011355. VP24. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05322-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKATGRYNL ISPKKDLEKG VVLSDLCNFL VSQTIQGWKV YWAGIEFDVT
60 70 80 90 100
HKGMALLHRL KTNDFAPAWS MTRNLFPHLF QNPNSTIESP LWALRVILAA
110 120 130 140 150
GIQDQLIDQS LIEPLAGALG LISDWLLTTN TNHFNMRTQR VKEQLSLKML
160 170 180 190 200
SLIRSNILKF INKLDALHVV NYNGLLSSIE IGTQNHTIII TRTNMGFLVE
210 220 230 240 250
LQEPDKSAMN RMKPGPAKFS LLHESTLKAF TQGSSTRMQS LILEFNSSLA

I
Length:251
Mass (Da):28,219
Last modified:July 15, 1998 - v2
Checksum:i5A8F356AF2CC5A5C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501T → I in strain: Isolate mouse-adapted.
Natural varianti71 – 711M → I in strain: Isolate guinea pig-adapted.
Natural varianti147 – 1471L → P in strain: Isolate guinea pig-adapted.
Natural varianti187 – 1871T → I in strain: Isolate guinea pig-adapted.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11365 Genomic RNA. Translation: AAB81006.1.
AF086833 Genomic RNA. Translation: AAD14588.1.
AF272001 Genomic RNA. Translation: AAG40170.1.
AY142960 Genomic RNA. Translation: AAN37510.1.
AF499101 Genomic RNA. Translation: AAM76037.1.
RefSeqiNP_066250.1. NC_002549.1.

Genome annotation databases

GeneIDi911828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11365 Genomic RNA. Translation: AAB81006.1 .
AF086833 Genomic RNA. Translation: AAD14588.1 .
AF272001 Genomic RNA. Translation: AAG40170.1 .
AY142960 Genomic RNA. Translation: AAN37510.1 .
AF499101 Genomic RNA. Translation: AAM76037.1 .
RefSeqi NP_066250.1. NC_002549.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4M0Q X-ray 1.92 A/B 11-237 [» ]
4U2X X-ray 3.15 A/B/C 16-231 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q05322. 36 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911828.

Family and domain databases

InterProi IPR009433. Filo_VP24.
[Graphical view ]
Pfami PF06389. Filo_VP24. 1 hit.
[Graphical view ]
PIRSFi PIRSF011355. VP24. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
    Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
    Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Characterization of the L gene and 5' trailer region of Ebola virus."
    Volchkov V.E., Volchkova V.A., Chepurnov A.A., Blinov V.M., Netesov S.V., Feldmann H.
    J. Gen. Virol. 80:355-362(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Molecular characterization of guinea pig-adapted variants of Ebola virus."
    Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
    Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate guinea pig-adapted.
  4. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate mouse-adapted.
  5. "The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
    Huang Y., Xu L., Sun Y., Nabel G.J.
    Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Biochemical and functional characterization of the Ebola virus VP24 protein: implications for a role in virus assembly and budding."
    Han Z., Boshra H., Sunyer J.O., Zwiers S.H., Paragas J., Harty R.N.
    J. Virol. 77:1793-1800(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles."
    Licata J.M., Johnson R.F., Han Z., Harty R.N.
    J. Virol. 78:7344-7351(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Ebola virus VP24 binds karyopherin alpha-1 and blocks STAT1 nuclear accumulation."
    Reid S.P., Leung L.W., Hartman A.L., Martinez O., Shaw M.L., Carbonnelle C., Volchkov V.E., Nichol S.T., Basler C.F.
    J. Virol. 80:5156-5167(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HOST KPNA1.
  9. "Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1."
    Reid S.P., Valmas C., Martinez O., Sanchez F.M., Basler C.F.
    J. Virol. 81:13469-13477(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HOST KPNA1.

Entry informationi

Entry nameiVP24_EBOZM
AccessioniPrimary (citable) accession number: Q05322
Secondary accession number(s): Q773N1, Q8JS60, Q9DQD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 15, 1998
Last modified: October 29, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3