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Q05322 (VP24_EBOZM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. Blocks the IFN-induced nuclear accumulation of host phosphorylated STAT1, by interacting with the STAT1-binding region of host importin alpha-1/KPNA1 protein, thereby inhibiting the latter. Without the activity of this protein, activated STAT1 would not enter the nucleus and be unable to activate IFN-induced genes. Plays a role in assembly of viral nucleocapsid and virion budding. May act as a minor matrix protein that plays a role in assembly of viral nucleocapsid and virion budding. Ref.5 Ref.6 Ref.7 Ref.9

Subunit structure

Binds to host importin alpha-1/KPNA1. Ref.8 Ref.9

Subcellular location

Virion membrane; Peripheral membrane protein. Host cell membrane; Peripheral membrane protein; Cytoplasmic side. Host endomembrane system; Peripheral membrane protein. Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and to the plasma membrane where budding takes place By similarity. Ref.6

Sequence similarities

Belongs to the filoviridae membrane-associated protein VP24 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Membrane-associated protein VP24
PRO_0000222154

Natural variations

Natural variant501T → I in strain: Isolate mouse-adapted.
Natural variant711M → I in strain: Isolate guinea pig-adapted.
Natural variant1471L → P in strain: Isolate guinea pig-adapted.
Natural variant1871T → I in strain: Isolate guinea pig-adapted.

Secondary structure

............................................... 251
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q05322 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 5A8F356AF2CC5A5C

FASTA25128,219
        10         20         30         40         50         60 
MAKATGRYNL ISPKKDLEKG VVLSDLCNFL VSQTIQGWKV YWAGIEFDVT HKGMALLHRL 

        70         80         90        100        110        120 
KTNDFAPAWS MTRNLFPHLF QNPNSTIESP LWALRVILAA GIQDQLIDQS LIEPLAGALG 

       130        140        150        160        170        180 
LISDWLLTTN TNHFNMRTQR VKEQLSLKML SLIRSNILKF INKLDALHVV NYNGLLSSIE 

       190        200        210        220        230        240 
IGTQNHTIII TRTNMGFLVE LQEPDKSAMN RMKPGPAKFS LLHESTLKAF TQGSSTRMQS 

       250 
LILEFNSSLA I 

« Hide

References

[1]"Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Characterization of the L gene and 5' trailer region of Ebola virus."
Volchkov V.E., Volchkova V.A., Chepurnov A.A., Blinov V.M., Netesov S.V., Feldmann H.
J. Gen. Virol. 80:355-362(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Molecular characterization of guinea pig-adapted variants of Ebola virus."
Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate guinea pig-adapted.
[4]Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate mouse-adapted.
[5]"The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
Huang Y., Xu L., Sun Y., Nabel G.J.
Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Biochemical and functional characterization of the Ebola virus VP24 protein: implications for a role in virus assembly and budding."
Han Z., Boshra H., Sunyer J.O., Zwiers S.H., Paragas J., Harty R.N.
J. Virol. 77:1793-1800(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles."
Licata J.M., Johnson R.F., Han Z., Harty R.N.
J. Virol. 78:7344-7351(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Ebola virus VP24 binds karyopherin alpha-1 and blocks STAT1 nuclear accumulation."
Reid S.P., Leung L.W., Hartman A.L., Martinez O., Shaw M.L., Carbonnelle C., Volchkov V.E., Nichol S.T., Basler C.F.
J. Virol. 80:5156-5167(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HOST KPNA1.
[9]"Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1."
Reid S.P., Valmas C., Martinez O., Sanchez F.M., Basler C.F.
J. Virol. 81:13469-13477(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HOST KPNA1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11365 Genomic RNA. Translation: AAB81006.1.
AF086833 Genomic RNA. Translation: AAD14588.1.
AF272001 Genomic RNA. Translation: AAG40170.1.
AY142960 Genomic RNA. Translation: AAN37510.1.
AF499101 Genomic RNA. Translation: AAM76037.1.
RefSeqNP_066250.1. NC_002549.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4M0QX-ray1.92A/B11-237[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ05322. 36 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID911828.

Family and domain databases

InterProIPR009433. Filo_VP24.
[Graphical view]
PfamPF06389. Filo_VP24. 1 hit.
[Graphical view]
PIRSFPIRSF011355. VP24. 1 hit.
ProtoNetSearch...

Entry information

Entry nameVP24_EBOZM
AccessionPrimary (citable) accession number: Q05322
Secondary accession number(s): Q773N1, Q8JS60, Q9DQD2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 15, 1998
Last modified: June 11, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references