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Protein

Membrane-associated protein VP24

Gene

VP24

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. Blocks the IFN-induced nuclear accumulation of host phosphorylated STAT1, by interacting with the STAT1-binding region of host importin alpha-1/KPNA1 protein, thereby inhibiting the latter. Without the activity of this protein, activated STAT1 would not enter the nucleus and be unable to activate IFN-induced genes. Plays a role in assembly of viral nucleocapsid and virion budding. May act as a minor matrix protein that plays a role in assembly of viral nucleocapsid and virion budding.4 Publications

GO - Molecular functioni

GO - Biological processi

  • suppression by virus of host intracellular interferon activity Source: CACAO
  • viral budding from plasma membrane Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Interferon antiviral system evasion

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated protein VP24
Gene namesi
Name:VP24
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Proteomesi
  • UP000007209 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell endomembrane system Source: UniProtKB-SubCell
  • host cell plasma membrane Source: CACAO
  • virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Membrane-associated protein VP24PRO_0000222154Add
BLAST

Interactioni

Subunit structurei

Binds to host importin alpha-1/KPNA1.

Protein-protein interaction databases

IntActiQ05322. 36 interactions.

Structurei

Secondary structure

1
251
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 2611Combined sources
Beta strandi30 – 334Combined sources
Beta strandi35 – 428Combined sources
Beta strandi45 – 506Combined sources
Helixi54 – 607Combined sources
Beta strandi62 – 654Combined sources
Turni67 – 693Combined sources
Helixi70 – 745Combined sources
Helixi77 – 804Combined sources
Beta strandi85 – 873Combined sources
Helixi90 – 10516Combined sources
Beta strandi108 – 1103Combined sources
Turni113 – 1153Combined sources
Helixi116 – 12813Combined sources
Helixi139 – 1424Combined sources
Helixi147 – 16519Combined sources
Beta strandi172 – 1743Combined sources
Beta strandi178 – 1825Combined sources
Beta strandi187 – 1937Combined sources
Beta strandi196 – 2027Combined sources
Helixi207 – 2093Combined sources
Beta strandi211 – 2133Combined sources
Beta strandi217 – 2226Combined sources
Helixi224 – 2285Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M0QX-ray1.92A/B11-237[»]
4U2XX-ray3.15A/B/C16-231[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR009433. Filo_VP24.
[Graphical view]
PfamiPF06389. Filo_VP24. 1 hit.
[Graphical view]
PIRSFiPIRSF011355. VP24. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKATGRYNL ISPKKDLEKG VVLSDLCNFL VSQTIQGWKV YWAGIEFDVT
60 70 80 90 100
HKGMALLHRL KTNDFAPAWS MTRNLFPHLF QNPNSTIESP LWALRVILAA
110 120 130 140 150
GIQDQLIDQS LIEPLAGALG LISDWLLTTN TNHFNMRTQR VKEQLSLKML
160 170 180 190 200
SLIRSNILKF INKLDALHVV NYNGLLSSIE IGTQNHTIII TRTNMGFLVE
210 220 230 240 250
LQEPDKSAMN RMKPGPAKFS LLHESTLKAF TQGSSTRMQS LILEFNSSLA

I
Length:251
Mass (Da):28,219
Last modified:July 15, 1998 - v2
Checksum:i5A8F356AF2CC5A5C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501T → I in strain: Isolate mouse-adapted.
Natural varianti71 – 711M → I in strain: Isolate guinea pig-adapted.
Natural varianti147 – 1471L → P in strain: Isolate guinea pig-adapted.
Natural varianti187 – 1871T → I in strain: Isolate guinea pig-adapted.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11365 Genomic RNA. Translation: AAB81006.1.
AF086833 Genomic RNA. Translation: AAD14588.1.
AF272001 Genomic RNA. Translation: AAG40170.1.
AY142960 Genomic RNA. Translation: AAN37510.1.
AF499101 Genomic RNA. Translation: AAM76037.1.
RefSeqiNP_066250.1. NC_002549.1.

Genome annotation databases

GeneIDi911828.
KEGGivg:911828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11365 Genomic RNA. Translation: AAB81006.1.
AF086833 Genomic RNA. Translation: AAD14588.1.
AF272001 Genomic RNA. Translation: AAG40170.1.
AY142960 Genomic RNA. Translation: AAN37510.1.
AF499101 Genomic RNA. Translation: AAM76037.1.
RefSeqiNP_066250.1. NC_002549.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M0QX-ray1.92A/B11-237[»]
4U2XX-ray3.15A/B/C16-231[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ05322. 36 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi911828.
KEGGivg:911828.

Family and domain databases

InterProiIPR009433. Filo_VP24.
[Graphical view]
PfamiPF06389. Filo_VP24. 1 hit.
[Graphical view]
PIRSFiPIRSF011355. VP24. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVP24_EBOZM
AccessioniPrimary (citable) accession number: Q05322
Secondary accession number(s): Q773N1, Q8JS60, Q9DQD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 15, 1998
Last modified: June 24, 2015
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.