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Q05318 (L_EBOZM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase L

Short name=Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

  1. RNA-directed RNA polymerase
    EC=2.7.7.48
  2. mRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.56
  3. mRNA guanylyltransferase
    EC=2.7.7.-
Gene names
Name:L
OrganismZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) [Reference proteome]
Taxonomic identifier128952 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length2212 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subcellular location

Virion. Host cytoplasm.

Sequence similarities

Contains 1 mononegavirus-type SAM-dependent 2'-O-MTase domain.

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 22122212RNA-directed RNA polymerase L
PRO_0000222167

Regions

Domain625 – 809185RdRp catalytic
Domain1805 – 2003199Mononegavirus-type SAM-dependent 2'-O-MTase

Natural variations

Natural variant8201T → A.
Natural variant9341F → L.
Natural variant15321I → V.

Sequences

Sequence LengthMass (Da)Tools
Q05318 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 5B07EDDC370E2934

FASTA2,212252,724
        10         20         30         40         50         60 
MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRNCKLPKHI YRLKYDVTVT 

        70         80         90        100        110        120 
KFLSDVPVAT LPIDFIVPVL LKALSGNGFC PVEPRCQQFL DEIIKYTMQD ALFLKYYLKN 

       130        140        150        160        170        180 
VGAQEDCVDE HFQEKILSSI QGNEFLHQMF FWYDLAILTR RGRLNRGNSR STWFVHDDLI 

       190        200        210        220        230        240 
DILGYGDYVF WKIPISMLPL NTQGIPHAAM DWYQASVFKE AVQGHTHIVS VSTADVLIMC 

       250        260        270        280        290        300 
KDLITCRFNT TLISKIAEIE DPVCSDYPNF KIVSMLYQSG DYLLSILGSD GYKIIKFLEP 

       310        320        330        340        350        360 
LCLAKIQLCS KYTERKGRFL TQMHLAVNHT LEEITEMRAL KPSQAQKIRE FHRTLIRLEM 

       370        380        390        400        410        420 
TPQQLCELFS IQKHWGHPVL HSETAIQKVK KHATVLKALR PIVIFETYCV FKYSIAKHYF 

       430        440        450        460        470        480 
DSQGSWYSVT SDRNLTPGLN SYIKRNQFPP LPMIKELLWE FYHLDHPPLF STKIISDLSI 

       490        500        510        520        530        540 
FIKDRATAVE RTCWDAVFEP NVLGYNPPHK FSTKRVPEQF LEQENFSIEN VLSYAQKLEY 

       550        560        570        580        590        600 
LLPQYRNFSF SLKEKELNVG RTFGKLPYPT RNVQTLCEAL LADGLAKAFP SNMMVVTERE 

       610        620        630        640        650        660 
QKESLLHQAS WHHTSDDFGE HATVRGSSFV TDLEKYNLAF RYEFTAPFIE YCNRCYGVKN 

       670        680        690        700        710        720 
VFNWMHYTIP QCYMHVSDYY NPPHNLTLEN RDNPPEGPSS YRGHMGGIEG LQQKLWTSIS 

       730        740        750        760        770        780 
CAQISLVEIK TGFKLRSAVM GDNQCITVLS VFPLETDADE QEQSAEDNAA RVAASLAKVT 

       790        800        810        820        830        840 
SACGIFLKPD ETFVHSGFIY FGKKQYLNGV QLPQSLKTAT RMAPLSDAIF DDLQGTLASI 

       850        860        870        880        890        900 
GTAFERSISE TRHIFPCRIT AAFHTFFSVR ILQYHHLGFN KGFDLGQLTL GKPLDFGTIS 

       910        920        930        940        950        960 
LALAVPQVLG GLSFLNPEKC FYRNLGDPVT SGLFQLKTYL RMIEMDDLFL PLIAKNPGNC 

       970        980        990       1000       1010       1020 
TAIDFVLNPS GLNVPGSQDL TSFLRQIVRR TITLSAKNKL INTLFHASAD FEDEMVCKWL 

      1030       1040       1050       1060       1070       1080 
LSSTPVMSRF AADIFSRTPS GKRLQILGYL EGTRTLLASK IINNNTETPV LDRLRKITLQ 

      1090       1100       1110       1120       1130       1140 
RWSLWFSYLD HCDNILAEAL TQITCTVDLA QILREYSWAH ILEGRPLIGA TLPCMIEQFK 

      1150       1160       1170       1180       1190       1200 
VFWLKPYEQC PQCSNAKQPG GKPFVSVAVK KHIVSAWPNA SRISWTIGDG IPYIGSRTED 

      1210       1220       1230       1240       1250       1260 
KIGQPAIKPK CPSAALREAI ELASRLTWVT QGSSNSDLLI KPFLEARVNL SVQEILQMTP 

      1270       1280       1290       1300       1310       1320 
SHYSGNIVHR YNDQYSPHSF MANRMSNSAT RLIVSTNTLG EFSGGGQSAR DSNIIFQNVI 

      1330       1340       1350       1360       1370       1380 
NYAVALFDIK FRNTEATDIQ YNRAHLHLTK CCTREVPAQY LTYTSTLDLD LTRYRENELI 

      1390       1400       1410       1420       1430       1440 
YDSNPLKGGL NCNISFDNPF FQGKRLNIIE DDLIRLPHLS GWELAKTIMQ SIISDSNNSS 

      1450       1460       1470       1480       1490       1500 
TDPISSGETR SFTTHFLTYP KIGLLYSFGA FVSYYLGNTI LRTKKLTLDN FLYYLTTQIH 

      1510       1520       1530       1540       1550       1560 
NLPHRSLRIL KPTFKHASVM SRLMSIDPHF SIYIGGAAGD RGLSDAARLF LRTSISSFLT 

      1570       1580       1590       1600       1610       1620 
FVKEWIINRG TIVPLWIVYP LEGQNPTPVN NFLYQIVELL VHDSSRQQAF KTTISDHVHP 

      1630       1640       1650       1660       1670       1680 
HDNLVYTCKS TASNFFHASL AYWRSRHRNS NRKYLARDSS TGSSTNNSDG HIERSQEQTT 

      1690       1700       1710       1720       1730       1740 
RDPHDGTERN LVLQMSHEIK RTTIPQENTH QGPSFQSFLS DSACGTANPK LNFDRSRHNV 

      1750       1760       1770       1780       1790       1800 
KFQDHNSASK REGHQIISHR LVLPFFTLSQ GTRQLTSSNE SQTQDEISKY LRQLRSVIDT 

      1810       1820       1830       1840       1850       1860 
TVYCRFTGIV SSMHYKLDEV LWEIESFKSA VTLAEGEGAG ALLLIQKYQV KTLFFNTLAT 

      1870       1880       1890       1900       1910       1920 
ESSIESEIVS GMTTPRMLLP VMSKFHNDQI EIILNNSASQ ITDITNPTWF KDQRARLPKQ 

      1930       1940       1950       1960       1970       1980 
VEVITMDAET TENINRSKLY EAVYKLILHH IDPSVLKAVV LKVFLSDTEG MLWLNDNLAP 

      1990       2000       2010       2020       2030       2040 
FFATGYLIKP ITSSARSSEW YLCLTNFLST TRKMPHQNHL SCKQVILTAL QLQIQRSPYW 

      2050       2060       2070       2080       2090       2100 
LSHLTQYADC ELHLSYIRLG FPSLEKVLYH RYNLVDSKRG PLVSITQHLA HLRAEIRELT 

      2110       2120       2130       2140       2150       2160 
NDYNQQRQSR TQTYHFIRTA KGRITKLVND YLKFFLIVQA LKHNGTWQAE FKKLPELISV 

      2170       2180       2190       2200       2210 
CNRFYHIRDC NCEERFLVQT LYLHRMQDSE VKLIERLTGL LSLFPDGLYR FD 

« Hide

References

[1]"Characterization of the L gene and 5' trailer region of Ebola virus."
Volchkov V.E., Volchkova V.A., Chepurnov A.A., Blinov V.M., Netesov S.V., Feldmann H.
J. Gen. Virol. 80:355-362(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-54.
[3]Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67110 Genomic RNA. Translation: CAA47483.1.
AF272001 Genomic RNA. Translation: AAG40171.1.
AF086833 Genomic RNA. Translation: AAD14589.1.
L11365 Genomic RNA. Translation: AAB81007.1.
AY142960 Genomic RNA. Translation: AAN37511.1.
AF499101 Genomic RNA. Translation: AAM76038.1.
RefSeqNP_066251.1. NC_002549.1.

3D structure databases

ProteinModelPortalQ05318.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID911824.

Family and domain databases

InterProIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsTIGR04198. paramyx_RNAcap. 2 hits.
PROSITEPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameL_EBOZM
AccessionPrimary (citable) accession number: Q05318
Secondary accession number(s): O39794 expand/collapse secondary AC list , Q773N0, Q8JS59, Q9DQD1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families