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Q05318

- L_EBOZM

UniProt

Q05318 - L_EBOZM

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Protein

RNA-directed RNA polymerase L

Gene
L
Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000007209: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22122212RNA-directed RNA polymerase LPRO_0000222167Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ05318.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini625 – 809185RdRp catalyticAdd
BLAST
Domaini1805 – 2003199Mononegavirus-type SAM-dependent 2'-O-MTaseAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsiTIGR04198. paramyx_RNAcap. 2 hits.
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05318-1 [UniParc]FASTAAdd to Basket

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MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRNCKLPKHI     50
YRLKYDVTVT KFLSDVPVAT LPIDFIVPVL LKALSGNGFC PVEPRCQQFL 100
DEIIKYTMQD ALFLKYYLKN VGAQEDCVDE HFQEKILSSI QGNEFLHQMF 150
FWYDLAILTR RGRLNRGNSR STWFVHDDLI DILGYGDYVF WKIPISMLPL 200
NTQGIPHAAM DWYQASVFKE AVQGHTHIVS VSTADVLIMC KDLITCRFNT 250
TLISKIAEIE DPVCSDYPNF KIVSMLYQSG DYLLSILGSD GYKIIKFLEP 300
LCLAKIQLCS KYTERKGRFL TQMHLAVNHT LEEITEMRAL KPSQAQKIRE 350
FHRTLIRLEM TPQQLCELFS IQKHWGHPVL HSETAIQKVK KHATVLKALR 400
PIVIFETYCV FKYSIAKHYF DSQGSWYSVT SDRNLTPGLN SYIKRNQFPP 450
LPMIKELLWE FYHLDHPPLF STKIISDLSI FIKDRATAVE RTCWDAVFEP 500
NVLGYNPPHK FSTKRVPEQF LEQENFSIEN VLSYAQKLEY LLPQYRNFSF 550
SLKEKELNVG RTFGKLPYPT RNVQTLCEAL LADGLAKAFP SNMMVVTERE 600
QKESLLHQAS WHHTSDDFGE HATVRGSSFV TDLEKYNLAF RYEFTAPFIE 650
YCNRCYGVKN VFNWMHYTIP QCYMHVSDYY NPPHNLTLEN RDNPPEGPSS 700
YRGHMGGIEG LQQKLWTSIS CAQISLVEIK TGFKLRSAVM GDNQCITVLS 750
VFPLETDADE QEQSAEDNAA RVAASLAKVT SACGIFLKPD ETFVHSGFIY 800
FGKKQYLNGV QLPQSLKTAT RMAPLSDAIF DDLQGTLASI GTAFERSISE 850
TRHIFPCRIT AAFHTFFSVR ILQYHHLGFN KGFDLGQLTL GKPLDFGTIS 900
LALAVPQVLG GLSFLNPEKC FYRNLGDPVT SGLFQLKTYL RMIEMDDLFL 950
PLIAKNPGNC TAIDFVLNPS GLNVPGSQDL TSFLRQIVRR TITLSAKNKL 1000
INTLFHASAD FEDEMVCKWL LSSTPVMSRF AADIFSRTPS GKRLQILGYL 1050
EGTRTLLASK IINNNTETPV LDRLRKITLQ RWSLWFSYLD HCDNILAEAL 1100
TQITCTVDLA QILREYSWAH ILEGRPLIGA TLPCMIEQFK VFWLKPYEQC 1150
PQCSNAKQPG GKPFVSVAVK KHIVSAWPNA SRISWTIGDG IPYIGSRTED 1200
KIGQPAIKPK CPSAALREAI ELASRLTWVT QGSSNSDLLI KPFLEARVNL 1250
SVQEILQMTP SHYSGNIVHR YNDQYSPHSF MANRMSNSAT RLIVSTNTLG 1300
EFSGGGQSAR DSNIIFQNVI NYAVALFDIK FRNTEATDIQ YNRAHLHLTK 1350
CCTREVPAQY LTYTSTLDLD LTRYRENELI YDSNPLKGGL NCNISFDNPF 1400
FQGKRLNIIE DDLIRLPHLS GWELAKTIMQ SIISDSNNSS TDPISSGETR 1450
SFTTHFLTYP KIGLLYSFGA FVSYYLGNTI LRTKKLTLDN FLYYLTTQIH 1500
NLPHRSLRIL KPTFKHASVM SRLMSIDPHF SIYIGGAAGD RGLSDAARLF 1550
LRTSISSFLT FVKEWIINRG TIVPLWIVYP LEGQNPTPVN NFLYQIVELL 1600
VHDSSRQQAF KTTISDHVHP HDNLVYTCKS TASNFFHASL AYWRSRHRNS 1650
NRKYLARDSS TGSSTNNSDG HIERSQEQTT RDPHDGTERN LVLQMSHEIK 1700
RTTIPQENTH QGPSFQSFLS DSACGTANPK LNFDRSRHNV KFQDHNSASK 1750
REGHQIISHR LVLPFFTLSQ GTRQLTSSNE SQTQDEISKY LRQLRSVIDT 1800
TVYCRFTGIV SSMHYKLDEV LWEIESFKSA VTLAEGEGAG ALLLIQKYQV 1850
KTLFFNTLAT ESSIESEIVS GMTTPRMLLP VMSKFHNDQI EIILNNSASQ 1900
ITDITNPTWF KDQRARLPKQ VEVITMDAET TENINRSKLY EAVYKLILHH 1950
IDPSVLKAVV LKVFLSDTEG MLWLNDNLAP FFATGYLIKP ITSSARSSEW 2000
YLCLTNFLST TRKMPHQNHL SCKQVILTAL QLQIQRSPYW LSHLTQYADC 2050
ELHLSYIRLG FPSLEKVLYH RYNLVDSKRG PLVSITQHLA HLRAEIRELT 2100
NDYNQQRQSR TQTYHFIRTA KGRITKLVND YLKFFLIVQA LKHNGTWQAE 2150
FKKLPELISV CNRFYHIRDC NCEERFLVQT LYLHRMQDSE VKLIERLTGL 2200
LSLFPDGLYR FD 2212
Length:2,212
Mass (Da):252,724
Last modified:May 30, 2000 - v2
Checksum:i5B07EDDC370E2934
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti820 – 8201T → A.
Natural varianti934 – 9341F → L.
Natural varianti1532 – 15321I → V.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67110 Genomic RNA. Translation: CAA47483.1.
AF272001 Genomic RNA. Translation: AAG40171.1.
AF086833 Genomic RNA. Translation: AAD14589.1.
L11365 Genomic RNA. Translation: AAB81007.1.
AY142960 Genomic RNA. Translation: AAN37511.1.
AF499101 Genomic RNA. Translation: AAM76038.1.
RefSeqiNP_066251.1. NC_002549.1.

Genome annotation databases

GeneIDi911824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67110 Genomic RNA. Translation: CAA47483.1 .
AF272001 Genomic RNA. Translation: AAG40171.1 .
AF086833 Genomic RNA. Translation: AAD14589.1 .
L11365 Genomic RNA. Translation: AAB81007.1 .
AY142960 Genomic RNA. Translation: AAN37511.1 .
AF499101 Genomic RNA. Translation: AAM76038.1 .
RefSeqi NP_066251.1. NC_002549.1.

3D structure databases

ProteinModelPortali Q05318.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911824.

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsi TIGR04198. paramyx_RNAcap. 2 hits.
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the L gene and 5' trailer region of Ebola virus."
    Volchkov V.E., Volchkova V.A., Chepurnov A.A., Blinov V.M., Netesov S.V., Feldmann H.
    J. Gen. Virol. 80:355-362(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
    Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
    Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-54.
  3. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiL_EBOZM
AccessioniPrimary (citable) accession number: Q05318
Secondary accession number(s): O39794
, Q773N0, Q8JS59, Q9DQD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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