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Q05318

- L_EBOZM

UniProt

Q05318 - L_EBOZM

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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000007209: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22122212RNA-directed RNA polymerase LPRO_0000222167Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ05318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini625 – 809185RdRp catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini1805 – 2003199Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 mononegavirus-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsiTIGR04198. paramyx_RNAcap. 2 hits.
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05318-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRNCKLPKHI
60 70 80 90 100
YRLKYDVTVT KFLSDVPVAT LPIDFIVPVL LKALSGNGFC PVEPRCQQFL
110 120 130 140 150
DEIIKYTMQD ALFLKYYLKN VGAQEDCVDE HFQEKILSSI QGNEFLHQMF
160 170 180 190 200
FWYDLAILTR RGRLNRGNSR STWFVHDDLI DILGYGDYVF WKIPISMLPL
210 220 230 240 250
NTQGIPHAAM DWYQASVFKE AVQGHTHIVS VSTADVLIMC KDLITCRFNT
260 270 280 290 300
TLISKIAEIE DPVCSDYPNF KIVSMLYQSG DYLLSILGSD GYKIIKFLEP
310 320 330 340 350
LCLAKIQLCS KYTERKGRFL TQMHLAVNHT LEEITEMRAL KPSQAQKIRE
360 370 380 390 400
FHRTLIRLEM TPQQLCELFS IQKHWGHPVL HSETAIQKVK KHATVLKALR
410 420 430 440 450
PIVIFETYCV FKYSIAKHYF DSQGSWYSVT SDRNLTPGLN SYIKRNQFPP
460 470 480 490 500
LPMIKELLWE FYHLDHPPLF STKIISDLSI FIKDRATAVE RTCWDAVFEP
510 520 530 540 550
NVLGYNPPHK FSTKRVPEQF LEQENFSIEN VLSYAQKLEY LLPQYRNFSF
560 570 580 590 600
SLKEKELNVG RTFGKLPYPT RNVQTLCEAL LADGLAKAFP SNMMVVTERE
610 620 630 640 650
QKESLLHQAS WHHTSDDFGE HATVRGSSFV TDLEKYNLAF RYEFTAPFIE
660 670 680 690 700
YCNRCYGVKN VFNWMHYTIP QCYMHVSDYY NPPHNLTLEN RDNPPEGPSS
710 720 730 740 750
YRGHMGGIEG LQQKLWTSIS CAQISLVEIK TGFKLRSAVM GDNQCITVLS
760 770 780 790 800
VFPLETDADE QEQSAEDNAA RVAASLAKVT SACGIFLKPD ETFVHSGFIY
810 820 830 840 850
FGKKQYLNGV QLPQSLKTAT RMAPLSDAIF DDLQGTLASI GTAFERSISE
860 870 880 890 900
TRHIFPCRIT AAFHTFFSVR ILQYHHLGFN KGFDLGQLTL GKPLDFGTIS
910 920 930 940 950
LALAVPQVLG GLSFLNPEKC FYRNLGDPVT SGLFQLKTYL RMIEMDDLFL
960 970 980 990 1000
PLIAKNPGNC TAIDFVLNPS GLNVPGSQDL TSFLRQIVRR TITLSAKNKL
1010 1020 1030 1040 1050
INTLFHASAD FEDEMVCKWL LSSTPVMSRF AADIFSRTPS GKRLQILGYL
1060 1070 1080 1090 1100
EGTRTLLASK IINNNTETPV LDRLRKITLQ RWSLWFSYLD HCDNILAEAL
1110 1120 1130 1140 1150
TQITCTVDLA QILREYSWAH ILEGRPLIGA TLPCMIEQFK VFWLKPYEQC
1160 1170 1180 1190 1200
PQCSNAKQPG GKPFVSVAVK KHIVSAWPNA SRISWTIGDG IPYIGSRTED
1210 1220 1230 1240 1250
KIGQPAIKPK CPSAALREAI ELASRLTWVT QGSSNSDLLI KPFLEARVNL
1260 1270 1280 1290 1300
SVQEILQMTP SHYSGNIVHR YNDQYSPHSF MANRMSNSAT RLIVSTNTLG
1310 1320 1330 1340 1350
EFSGGGQSAR DSNIIFQNVI NYAVALFDIK FRNTEATDIQ YNRAHLHLTK
1360 1370 1380 1390 1400
CCTREVPAQY LTYTSTLDLD LTRYRENELI YDSNPLKGGL NCNISFDNPF
1410 1420 1430 1440 1450
FQGKRLNIIE DDLIRLPHLS GWELAKTIMQ SIISDSNNSS TDPISSGETR
1460 1470 1480 1490 1500
SFTTHFLTYP KIGLLYSFGA FVSYYLGNTI LRTKKLTLDN FLYYLTTQIH
1510 1520 1530 1540 1550
NLPHRSLRIL KPTFKHASVM SRLMSIDPHF SIYIGGAAGD RGLSDAARLF
1560 1570 1580 1590 1600
LRTSISSFLT FVKEWIINRG TIVPLWIVYP LEGQNPTPVN NFLYQIVELL
1610 1620 1630 1640 1650
VHDSSRQQAF KTTISDHVHP HDNLVYTCKS TASNFFHASL AYWRSRHRNS
1660 1670 1680 1690 1700
NRKYLARDSS TGSSTNNSDG HIERSQEQTT RDPHDGTERN LVLQMSHEIK
1710 1720 1730 1740 1750
RTTIPQENTH QGPSFQSFLS DSACGTANPK LNFDRSRHNV KFQDHNSASK
1760 1770 1780 1790 1800
REGHQIISHR LVLPFFTLSQ GTRQLTSSNE SQTQDEISKY LRQLRSVIDT
1810 1820 1830 1840 1850
TVYCRFTGIV SSMHYKLDEV LWEIESFKSA VTLAEGEGAG ALLLIQKYQV
1860 1870 1880 1890 1900
KTLFFNTLAT ESSIESEIVS GMTTPRMLLP VMSKFHNDQI EIILNNSASQ
1910 1920 1930 1940 1950
ITDITNPTWF KDQRARLPKQ VEVITMDAET TENINRSKLY EAVYKLILHH
1960 1970 1980 1990 2000
IDPSVLKAVV LKVFLSDTEG MLWLNDNLAP FFATGYLIKP ITSSARSSEW
2010 2020 2030 2040 2050
YLCLTNFLST TRKMPHQNHL SCKQVILTAL QLQIQRSPYW LSHLTQYADC
2060 2070 2080 2090 2100
ELHLSYIRLG FPSLEKVLYH RYNLVDSKRG PLVSITQHLA HLRAEIRELT
2110 2120 2130 2140 2150
NDYNQQRQSR TQTYHFIRTA KGRITKLVND YLKFFLIVQA LKHNGTWQAE
2160 2170 2180 2190 2200
FKKLPELISV CNRFYHIRDC NCEERFLVQT LYLHRMQDSE VKLIERLTGL
2210
LSLFPDGLYR FD
Length:2,212
Mass (Da):252,724
Last modified:May 30, 2000 - v2
Checksum:i5B07EDDC370E2934
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti820 – 8201T → A.
Natural varianti934 – 9341F → L.
Natural varianti1532 – 15321I → V.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67110 Genomic RNA. Translation: CAA47483.1.
AF272001 Genomic RNA. Translation: AAG40171.1.
AF086833 Genomic RNA. Translation: AAD14589.1.
L11365 Genomic RNA. Translation: AAB81007.1.
AY142960 Genomic RNA. Translation: AAN37511.1.
AF499101 Genomic RNA. Translation: AAM76038.1.
RefSeqiNP_066251.1. NC_002549.1.

Genome annotation databases

GeneIDi911824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67110 Genomic RNA. Translation: CAA47483.1 .
AF272001 Genomic RNA. Translation: AAG40171.1 .
AF086833 Genomic RNA. Translation: AAD14589.1 .
L11365 Genomic RNA. Translation: AAB81007.1 .
AY142960 Genomic RNA. Translation: AAN37511.1 .
AF499101 Genomic RNA. Translation: AAM76038.1 .
RefSeqi NP_066251.1. NC_002549.1.

3D structure databases

ProteinModelPortali Q05318.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911824.

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsi TIGR04198. paramyx_RNAcap. 2 hits.
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the L gene and 5' trailer region of Ebola virus."
    Volchkov V.E., Volchkova V.A., Chepurnov A.A., Blinov V.M., Netesov S.V., Feldmann H.
    J. Gen. Virol. 80:355-362(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
    Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
    Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-54.
  3. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiL_EBOZM
AccessioniPrimary (citable) accession number: Q05318
Secondary accession number(s): O39794
, Q773N0, Q8JS59, Q9DQD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3