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Q05318

- L_EBOZM

UniProt

Q05318 - L_EBOZM

Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Including the following 3 domains:
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    mRNA guanylyltransferase (EC:2.7.7.-)
    Gene namesi
    Name:L
    OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
    Taxonomic identifieri128952 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    ProteomesiUP000007209: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22122212RNA-directed RNA polymerase LPRO_0000222167Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ05318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini625 – 809185RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini1805 – 2003199Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 mononegavirus-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR017235. RNA-dir_pol_L_filovirus.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view]
    PfamiPF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
    TIGRFAMsiTIGR04198. paramyx_RNAcap. 2 hits.
    PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    PS51590. SAM_MT_MNV_L. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q05318-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRNCKLPKHI     50
    YRLKYDVTVT KFLSDVPVAT LPIDFIVPVL LKALSGNGFC PVEPRCQQFL 100
    DEIIKYTMQD ALFLKYYLKN VGAQEDCVDE HFQEKILSSI QGNEFLHQMF 150
    FWYDLAILTR RGRLNRGNSR STWFVHDDLI DILGYGDYVF WKIPISMLPL 200
    NTQGIPHAAM DWYQASVFKE AVQGHTHIVS VSTADVLIMC KDLITCRFNT 250
    TLISKIAEIE DPVCSDYPNF KIVSMLYQSG DYLLSILGSD GYKIIKFLEP 300
    LCLAKIQLCS KYTERKGRFL TQMHLAVNHT LEEITEMRAL KPSQAQKIRE 350
    FHRTLIRLEM TPQQLCELFS IQKHWGHPVL HSETAIQKVK KHATVLKALR 400
    PIVIFETYCV FKYSIAKHYF DSQGSWYSVT SDRNLTPGLN SYIKRNQFPP 450
    LPMIKELLWE FYHLDHPPLF STKIISDLSI FIKDRATAVE RTCWDAVFEP 500
    NVLGYNPPHK FSTKRVPEQF LEQENFSIEN VLSYAQKLEY LLPQYRNFSF 550
    SLKEKELNVG RTFGKLPYPT RNVQTLCEAL LADGLAKAFP SNMMVVTERE 600
    QKESLLHQAS WHHTSDDFGE HATVRGSSFV TDLEKYNLAF RYEFTAPFIE 650
    YCNRCYGVKN VFNWMHYTIP QCYMHVSDYY NPPHNLTLEN RDNPPEGPSS 700
    YRGHMGGIEG LQQKLWTSIS CAQISLVEIK TGFKLRSAVM GDNQCITVLS 750
    VFPLETDADE QEQSAEDNAA RVAASLAKVT SACGIFLKPD ETFVHSGFIY 800
    FGKKQYLNGV QLPQSLKTAT RMAPLSDAIF DDLQGTLASI GTAFERSISE 850
    TRHIFPCRIT AAFHTFFSVR ILQYHHLGFN KGFDLGQLTL GKPLDFGTIS 900
    LALAVPQVLG GLSFLNPEKC FYRNLGDPVT SGLFQLKTYL RMIEMDDLFL 950
    PLIAKNPGNC TAIDFVLNPS GLNVPGSQDL TSFLRQIVRR TITLSAKNKL 1000
    INTLFHASAD FEDEMVCKWL LSSTPVMSRF AADIFSRTPS GKRLQILGYL 1050
    EGTRTLLASK IINNNTETPV LDRLRKITLQ RWSLWFSYLD HCDNILAEAL 1100
    TQITCTVDLA QILREYSWAH ILEGRPLIGA TLPCMIEQFK VFWLKPYEQC 1150
    PQCSNAKQPG GKPFVSVAVK KHIVSAWPNA SRISWTIGDG IPYIGSRTED 1200
    KIGQPAIKPK CPSAALREAI ELASRLTWVT QGSSNSDLLI KPFLEARVNL 1250
    SVQEILQMTP SHYSGNIVHR YNDQYSPHSF MANRMSNSAT RLIVSTNTLG 1300
    EFSGGGQSAR DSNIIFQNVI NYAVALFDIK FRNTEATDIQ YNRAHLHLTK 1350
    CCTREVPAQY LTYTSTLDLD LTRYRENELI YDSNPLKGGL NCNISFDNPF 1400
    FQGKRLNIIE DDLIRLPHLS GWELAKTIMQ SIISDSNNSS TDPISSGETR 1450
    SFTTHFLTYP KIGLLYSFGA FVSYYLGNTI LRTKKLTLDN FLYYLTTQIH 1500
    NLPHRSLRIL KPTFKHASVM SRLMSIDPHF SIYIGGAAGD RGLSDAARLF 1550
    LRTSISSFLT FVKEWIINRG TIVPLWIVYP LEGQNPTPVN NFLYQIVELL 1600
    VHDSSRQQAF KTTISDHVHP HDNLVYTCKS TASNFFHASL AYWRSRHRNS 1650
    NRKYLARDSS TGSSTNNSDG HIERSQEQTT RDPHDGTERN LVLQMSHEIK 1700
    RTTIPQENTH QGPSFQSFLS DSACGTANPK LNFDRSRHNV KFQDHNSASK 1750
    REGHQIISHR LVLPFFTLSQ GTRQLTSSNE SQTQDEISKY LRQLRSVIDT 1800
    TVYCRFTGIV SSMHYKLDEV LWEIESFKSA VTLAEGEGAG ALLLIQKYQV 1850
    KTLFFNTLAT ESSIESEIVS GMTTPRMLLP VMSKFHNDQI EIILNNSASQ 1900
    ITDITNPTWF KDQRARLPKQ VEVITMDAET TENINRSKLY EAVYKLILHH 1950
    IDPSVLKAVV LKVFLSDTEG MLWLNDNLAP FFATGYLIKP ITSSARSSEW 2000
    YLCLTNFLST TRKMPHQNHL SCKQVILTAL QLQIQRSPYW LSHLTQYADC 2050
    ELHLSYIRLG FPSLEKVLYH RYNLVDSKRG PLVSITQHLA HLRAEIRELT 2100
    NDYNQQRQSR TQTYHFIRTA KGRITKLVND YLKFFLIVQA LKHNGTWQAE 2150
    FKKLPELISV CNRFYHIRDC NCEERFLVQT LYLHRMQDSE VKLIERLTGL 2200
    LSLFPDGLYR FD 2212
    Length:2,212
    Mass (Da):252,724
    Last modified:May 30, 2000 - v2
    Checksum:i5B07EDDC370E2934
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti820 – 8201T → A.
    Natural varianti934 – 9341F → L.
    Natural varianti1532 – 15321I → V.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67110 Genomic RNA. Translation: CAA47483.1.
    AF272001 Genomic RNA. Translation: AAG40171.1.
    AF086833 Genomic RNA. Translation: AAD14589.1.
    L11365 Genomic RNA. Translation: AAB81007.1.
    AY142960 Genomic RNA. Translation: AAN37511.1.
    AF499101 Genomic RNA. Translation: AAM76038.1.
    RefSeqiNP_066251.1. NC_002549.1.

    Genome annotation databases

    GeneIDi911824.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67110 Genomic RNA. Translation: CAA47483.1 .
    AF272001 Genomic RNA. Translation: AAG40171.1 .
    AF086833 Genomic RNA. Translation: AAD14589.1 .
    L11365 Genomic RNA. Translation: AAB81007.1 .
    AY142960 Genomic RNA. Translation: AAN37511.1 .
    AF499101 Genomic RNA. Translation: AAM76038.1 .
    RefSeqi NP_066251.1. NC_002549.1.

    3D structure databases

    ProteinModelPortali Q05318.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 911824.

    Family and domain databases

    InterProi IPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR017235. RNA-dir_pol_L_filovirus.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view ]
    Pfami PF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
    TIGRFAMsi TIGR04198. paramyx_RNAcap. 2 hits.
    PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    PS51590. SAM_MT_MNV_L. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the L gene and 5' trailer region of Ebola virus."
      Volchkov V.E., Volchkova V.A., Chepurnov A.A., Blinov V.M., Netesov S.V., Feldmann H.
      J. Gen. Virol. 80:355-362(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
      Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
      Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-54.
    3. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiL_EBOZM
    AccessioniPrimary (citable) accession number: Q05318
    Secondary accession number(s): O39794
    , Q773N0, Q8JS59, Q9DQD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3