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Q05315

- LEG10_HUMAN

UniProt

Q05315 - LEG10_HUMAN

Protein

Galectin-10

Gene

CLC

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei136 – 1361Not glycosylated

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. multicellular organismal development Source: ProtInc
    2. regulation of activated T cell proliferation Source: UniProtKB
    3. regulation of T cell anergy Source: UniProtKB
    4. regulation of T cell cytokine production Source: UniProtKB
    5. T cell apoptotic process Source: UniProtKB

    Keywords - Ligandi

    Lectin

    Enzyme and pathway databases

    BRENDAi3.1.1.5. 2681.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galectin-10
    Short name:
    Gal-10
    Alternative name(s):
    Charcot-Leyden crystal protein
    Short name:
    CLC
    Eosinophil lysophospholipase
    Lysolecithin acylhydrolase
    Gene namesi
    Name:CLC
    Synonyms:LGALS10, LGALS10A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:2014. CLC.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Cytoplasmic granule 1 Publication
    Note: Localized in granules from where it may be secreted or transported to other locations in the cell.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26541.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 142141Galectin-10PRO_0000076960Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ05315.
    PRIDEiQ05315.

    PTM databases

    PhosphoSiteiQ05315.

    Expressioni

    Tissue specificityi

    Expressed abundantly in the bone marrow. Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. Expressed in CD25-positive regulatory T-cells (Treg) (at protein level). Found in intestinal tissue from patients with Celiac disease, expression is directly related to the histological grade of mucosal damage and to the number of eosinophils found in the duodenal lesion (at protein level). Found in sputum of patients with eosinophilic inflammatory diseases such as asthma (at protein level).4 Publications

    Gene expression databases

    ArrayExpressiQ05315.
    BgeeiQ05315.
    CleanExiHS_CLC.
    GenevestigatoriQ05315.

    Organism-specific databases

    HPAiHPA041751.

    Interactioni

    Subunit structurei

    Interacts with CEL.1 Publication

    Protein-protein interaction databases

    STRINGi9606.ENSP00000221804.

    Structurei

    Secondary structure

    1
    142
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 116
    Beta strandi19 – 2810
    Helixi30 – 323
    Beta strandi35 – 4511
    Beta strandi50 – 578
    Turni58 – 603
    Beta strandi61 – 688
    Beta strandi76 – 783
    Beta strandi89 – 957
    Beta strandi97 – 1048
    Beta strandi107 – 1137
    Helixi118 – 1203
    Beta strandi123 – 13715

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G86X-ray1.80A1-142[»]
    1HDKX-ray1.80A2-140[»]
    1LCLX-ray1.80A1-142[»]
    1QKQX-ray1.80A1-142[»]
    ProteinModelPortaliQ05315.
    SMRiQ05315. Positions 2-142.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ05315.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 138133GalectinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 galectin domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG307598.
    HOGENOMiHOG000015090.
    HOVERGENiHBG006255.
    InParanoidiQ05315.
    KOiK13334.
    OMAiFHTEMKE.
    OrthoDBiEOG7WQ7V1.
    PhylomeDBiQ05315.
    TreeFamiTF315551.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR001079. Galectin_CRD.
    [Graphical view]
    PfamiPF00337. Gal-bind_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00908. Gal-bind_lectin. 1 hit.
    SM00276. GLECT. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS51304. GALECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05315-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI    50
    VFHFQVCFGR RVVMNSREYG AWKQQVESKN MPFQDGQEFE LSISVLPDKY 100
    QVMVNGQSSY TFDHRIKPEA VKMVQVWRDI SLTKFNVSYL KR 142
    Length:142
    Mass (Da):16,453
    Last modified:January 11, 2011 - v3
    Checksum:i2744F0C082C6446E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti28 – 281A → V.6 Publications
    Corresponds to variant rs17608 [ dbSNP | Ensembl ].
    VAR_014765

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01664 mRNA. Translation: AAA36190.1.
    L01665 Genomic DNA. Translation: AAC37530.1.
    U68398
    , U68393, U68395, U68396 Genomic DNA. Translation: AAC51157.1.
    FJ613334 mRNA. Translation: ACR09636.1.
    FJ613335 mRNA. Translation: ACR09637.1.
    AC005393 Genomic DNA. Translation: AAC28912.1.
    AC006133 Genomic DNA. No translation available.
    CH471126 Genomic DNA. Translation: EAW56914.1.
    BC119711 mRNA. Translation: AAI19712.1.
    BC119712 mRNA. Translation: AAI19713.1.
    CCDSiCCDS33025.1.
    PIRiA46523.
    RefSeqiNP_001819.2. NM_001828.5.
    UniGeneiHs.889.

    Genome annotation databases

    EnsembliENST00000221804; ENSP00000221804; ENSG00000105205.
    GeneIDi1178.
    KEGGihsa:1178.
    UCSCiuc002omh.3. human.

    Polymorphism databases

    DMDMi317373429.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    Galectin-10

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01664 mRNA. Translation: AAA36190.1 .
    L01665 Genomic DNA. Translation: AAC37530.1 .
    U68398
    , U68393 , U68395 , U68396 Genomic DNA. Translation: AAC51157.1 .
    FJ613334 mRNA. Translation: ACR09636.1 .
    FJ613335 mRNA. Translation: ACR09637.1 .
    AC005393 Genomic DNA. Translation: AAC28912.1 .
    AC006133 Genomic DNA. No translation available.
    CH471126 Genomic DNA. Translation: EAW56914.1 .
    BC119711 mRNA. Translation: AAI19712.1 .
    BC119712 mRNA. Translation: AAI19713.1 .
    CCDSi CCDS33025.1.
    PIRi A46523.
    RefSeqi NP_001819.2. NM_001828.5.
    UniGenei Hs.889.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G86 X-ray 1.80 A 1-142 [» ]
    1HDK X-ray 1.80 A 2-140 [» ]
    1LCL X-ray 1.80 A 1-142 [» ]
    1QKQ X-ray 1.80 A 1-142 [» ]
    ProteinModelPortali Q05315.
    SMRi Q05315. Positions 2-142.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000221804.

    PTM databases

    PhosphoSitei Q05315.

    Polymorphism databases

    DMDMi 317373429.

    Proteomic databases

    PaxDbi Q05315.
    PRIDEi Q05315.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000221804 ; ENSP00000221804 ; ENSG00000105205 .
    GeneIDi 1178.
    KEGGi hsa:1178.
    UCSCi uc002omh.3. human.

    Organism-specific databases

    CTDi 1178.
    GeneCardsi GC19M040221.
    H-InvDB HIX0040154.
    HGNCi HGNC:2014. CLC.
    HPAi HPA041751.
    MIMi 153310. gene.
    neXtProti NX_Q05315.
    PharmGKBi PA26541.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG307598.
    HOGENOMi HOG000015090.
    HOVERGENi HBG006255.
    InParanoidi Q05315.
    KOi K13334.
    OMAi FHTEMKE.
    OrthoDBi EOG7WQ7V1.
    PhylomeDBi Q05315.
    TreeFami TF315551.

    Enzyme and pathway databases

    BRENDAi 3.1.1.5. 2681.

    Miscellaneous databases

    EvolutionaryTracei Q05315.
    GeneWikii CLC_(gene).
    GenomeRNAii 1178.
    NextBioi 35482744.
    PROi Q05315.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q05315.
    Bgeei Q05315.
    CleanExi HS_CLC.
    Genevestigatori Q05315.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR001079. Galectin_CRD.
    [Graphical view ]
    Pfami PF00337. Gal-bind_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00908. Gal-bind_lectin. 1 hit.
    SM00276. GLECT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS51304. GALECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase). Similarities to IgE binding proteins and the S-type animal lectin superfamily."
      Ackerman S.J., Corrette S.E., Rosenberg H.F., Bennett J.C., Mastrianni D.M., Nicholson-Weller A., Weller P.F., Chin D.T., Tenen D.G.
      J. Immunol. 150:456-468(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-18 AND 93-110, VARIANT VAL-28.
    2. "Localization of the human eosinophil Charcot-Leyden crystal protein (lysophospholipase) gene (CLC) to chromosome 19 and the human ribonuclease 2 (eosinophil-derived neurotoxin) and ribonuclease 3 (eosinophil cationic protein) genes (RNS2 and RNS3) to chromosome 14."
      Mastrianni D.M., Eddy R.L., Rosenberg H.F., Corrette S.E., Shows T.B., Tenen D.G., Ackerman S.J.
      Genomics 13:240-242(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-28.
    3. "The genomic structure of the human Charcot-Leyden crystal protein gene is analogous to those of the galectin genes."
      Dyer K.D., Handen J.S., Rosenberg H.F.
      Genomics 40:217-221(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-28.
    4. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT VAL-28.
      Tissue: Placenta.
    5. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-28.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-28.
    8. "Comparative proteomics of nasal fluid in seasonal allergic rhinitis."
      Ghafouri B., Irander K., Lindbom J., Tagesson C., Lindahl M.
      J. Proteome Res. 5:330-338(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-23; 92-99 AND 135-141, ACETYLATION AT SER-2, LACK OF GLYCOSYLATION AT ASN-136, IDENTIFICATION BY MASS SPECTROMETRY.
    9. "Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function."
      Kubach J., Lutter P., Bopp T., Stoll S., Becker C., Huter E., Richter C., Weingarten P., Warger T., Knop J., Muellner S., Wijdenes J., Schild H., Schmitt E., Jonuleit H.
      Blood 110:1550-1558(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    10. Cited for: TISSUE SPECIFICITY.
    11. "Galectin-10, a potential biomarker of eosinophilic airway inflammation."
      Chua J.C., Douglass J.A., Gillman A., O'Hehir R.E., Meeusen E.N.
      PLoS ONE 7:E42549-E42549(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    12. "Crystal structure of human Charcot-Leyden crystal protein, an eosinophil lysophospholipase, identifies it as a new member of the carbohydrate-binding family of galectins."
      Leonidas D.D., Elbert B.L., Zhou Z., Leffler H., Ackerman S.J., Acharya K.R.
      Structure 3:1379-1393(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
    13. "Selective recognition of mannose by the human eosinophil Charcot-Leyden crystal protein (galectin-10): a crystallographic study at 1.8 A resolution."
      Swaminathan G.J., Leonidas D.D., Savage M.P., Ackerman S.J., Acharya K.R.
      Biochemistry 38:13837-13843(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
    14. "Charcot-Leyden crystal protein (galectin-10) is not a dual function galectin with lysophospholipase activity but binds a lysophospholipase inhibitor in a novel structural fashion."
      Ackerman S.J., Liu L., Kwatia M.A., Savage M.P., Leonidas D.D., Swaminathan G.J., Acharya K.R.
      J. Biol. Chem. 277:14859-14868(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 2-139, ABSENCE OF LYSOPHOSPHOLIPASE ACTIVITY, INTERACTION WITH CEL.

    Entry informationi

    Entry nameiLEG10_HUMAN
    AccessioniPrimary (citable) accession number: Q05315
    Secondary accession number(s): C5HZ13, C5HZ14, Q0VDE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 143 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Forms hexagonal bipyramidal crystals, known as Charcot-Leyden crystals, in tissues and secretions from sites of eosinophil-associated inflammation and some myeloid leukemias.

    Caution

    Was originally thought to possess lysophospholipase activity but the absence of this activity has been shown by PubMed:11834744.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3