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Q05315

- LEG10_HUMAN

UniProt

Q05315 - LEG10_HUMAN

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Protein

Galectin-10

Gene

CLC

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei136 – 1361Not glycosylated

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB

GO - Biological processi

  1. multicellular organismal development Source: ProtInc
  2. regulation of activated T cell proliferation Source: UniProtKB
  3. regulation of T cell anergy Source: UniProtKB
  4. regulation of T cell cytokine production Source: UniProtKB
  5. T cell apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Lectin

Enzyme and pathway databases

BRENDAi3.1.1.5. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-10
Short name:
Gal-10
Alternative name(s):
Charcot-Leyden crystal protein
Short name:
CLC
Eosinophil lysophospholipase
Lysolecithin acylhydrolase
Gene namesi
Name:CLC
Synonyms:LGALS10, LGALS10A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:2014. CLC.

Subcellular locationi

Cytoplasmcytosol 1 Publication. Cytoplasmic granule 1 Publication
Note: Localized in granules from where it may be secreted or transported to other locations in the cell.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26541.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 142141Galectin-10PRO_0000076960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ05315.
PRIDEiQ05315.

PTM databases

PhosphoSiteiQ05315.

Expressioni

Tissue specificityi

Expressed abundantly in the bone marrow. Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. Expressed in CD25-positive regulatory T-cells (Treg) (at protein level). Found in intestinal tissue from patients with Celiac disease, expression is directly related to the histological grade of mucosal damage and to the number of eosinophils found in the duodenal lesion (at protein level). Found in sputum of patients with eosinophilic inflammatory diseases such as asthma (at protein level).4 Publications

Gene expression databases

BgeeiQ05315.
CleanExiHS_CLC.
GenevestigatoriQ05315.

Organism-specific databases

HPAiHPA041751.

Interactioni

Subunit structurei

Interacts with CEL.1 Publication

Protein-protein interaction databases

STRINGi9606.ENSP00000221804.

Structurei

Secondary structure

1
142
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 116Combined sources
Beta strandi19 – 2810Combined sources
Helixi30 – 323Combined sources
Beta strandi35 – 4511Combined sources
Beta strandi50 – 578Combined sources
Turni58 – 603Combined sources
Beta strandi61 – 688Combined sources
Beta strandi76 – 783Combined sources
Beta strandi89 – 957Combined sources
Beta strandi97 – 1048Combined sources
Beta strandi107 – 1137Combined sources
Helixi118 – 1203Combined sources
Beta strandi123 – 13715Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G86X-ray1.80A1-142[»]
1HDKX-ray1.80A2-140[»]
1LCLX-ray1.80A1-142[»]
1QKQX-ray1.80A1-142[»]
ProteinModelPortaliQ05315.
SMRiQ05315. Positions 2-142.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05315.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 138133GalectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 galectin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG307598.
GeneTreeiENSGT00530000064497.
HOGENOMiHOG000015090.
HOVERGENiHBG006255.
InParanoidiQ05315.
KOiK13334.
OMAiFHTEMKE.
OrthoDBiEOG7WQ7V1.
PhylomeDBiQ05315.
TreeFamiTF315551.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
[Graphical view]
PfamiPF00337. Gal-bind_lectin. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05315-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI
60 70 80 90 100
VFHFQVCFGR RVVMNSREYG AWKQQVESKN MPFQDGQEFE LSISVLPDKY
110 120 130 140
QVMVNGQSSY TFDHRIKPEA VKMVQVWRDI SLTKFNVSYL KR
Length:142
Mass (Da):16,453
Last modified:January 11, 2011 - v3
Checksum:i2744F0C082C6446E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti28 – 281A → V.6 Publications
Corresponds to variant rs17608 [ dbSNP | Ensembl ].
VAR_014765

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01664 mRNA. Translation: AAA36190.1.
L01665 Genomic DNA. Translation: AAC37530.1.
U68398
, U68393, U68395, U68396 Genomic DNA. Translation: AAC51157.1.
FJ613334 mRNA. Translation: ACR09636.1.
FJ613335 mRNA. Translation: ACR09637.1.
AC005393 Genomic DNA. Translation: AAC28912.1.
AC006133 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW56914.1.
BC119711 mRNA. Translation: AAI19712.1.
BC119712 mRNA. Translation: AAI19713.1.
CCDSiCCDS33025.1.
PIRiA46523.
RefSeqiNP_001819.2. NM_001828.5.
UniGeneiHs.889.

Genome annotation databases

EnsembliENST00000221804; ENSP00000221804; ENSG00000105205.
GeneIDi1178.
KEGGihsa:1178.
UCSCiuc002omh.3. human.

Polymorphism databases

DMDMi317373429.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Galectin-10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01664 mRNA. Translation: AAA36190.1 .
L01665 Genomic DNA. Translation: AAC37530.1 .
U68398
, U68393 , U68395 , U68396 Genomic DNA. Translation: AAC51157.1 .
FJ613334 mRNA. Translation: ACR09636.1 .
FJ613335 mRNA. Translation: ACR09637.1 .
AC005393 Genomic DNA. Translation: AAC28912.1 .
AC006133 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW56914.1 .
BC119711 mRNA. Translation: AAI19712.1 .
BC119712 mRNA. Translation: AAI19713.1 .
CCDSi CCDS33025.1.
PIRi A46523.
RefSeqi NP_001819.2. NM_001828.5.
UniGenei Hs.889.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G86 X-ray 1.80 A 1-142 [» ]
1HDK X-ray 1.80 A 2-140 [» ]
1LCL X-ray 1.80 A 1-142 [» ]
1QKQ X-ray 1.80 A 1-142 [» ]
ProteinModelPortali Q05315.
SMRi Q05315. Positions 2-142.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000221804.

PTM databases

PhosphoSitei Q05315.

Polymorphism databases

DMDMi 317373429.

Proteomic databases

PaxDbi Q05315.
PRIDEi Q05315.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000221804 ; ENSP00000221804 ; ENSG00000105205 .
GeneIDi 1178.
KEGGi hsa:1178.
UCSCi uc002omh.3. human.

Organism-specific databases

CTDi 1178.
GeneCardsi GC19M040221.
H-InvDB HIX0040154.
HGNCi HGNC:2014. CLC.
HPAi HPA041751.
MIMi 153310. gene.
neXtProti NX_Q05315.
PharmGKBi PA26541.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG307598.
GeneTreei ENSGT00530000064497.
HOGENOMi HOG000015090.
HOVERGENi HBG006255.
InParanoidi Q05315.
KOi K13334.
OMAi FHTEMKE.
OrthoDBi EOG7WQ7V1.
PhylomeDBi Q05315.
TreeFami TF315551.

Enzyme and pathway databases

BRENDAi 3.1.1.5. 2681.

Miscellaneous databases

EvolutionaryTracei Q05315.
GeneWikii CLC_(gene).
GenomeRNAii 1178.
NextBioi 35482744.
PROi Q05315.
SOURCEi Search...

Gene expression databases

Bgeei Q05315.
CleanExi HS_CLC.
Genevestigatori Q05315.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
[Graphical view ]
Pfami PF00337. Gal-bind_lectin. 1 hit.
[Graphical view ]
SMARTi SM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS51304. GALECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase). Similarities to IgE binding proteins and the S-type animal lectin superfamily."
    Ackerman S.J., Corrette S.E., Rosenberg H.F., Bennett J.C., Mastrianni D.M., Nicholson-Weller A., Weller P.F., Chin D.T., Tenen D.G.
    J. Immunol. 150:456-468(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-18 AND 93-110, VARIANT VAL-28.
  2. "Localization of the human eosinophil Charcot-Leyden crystal protein (lysophospholipase) gene (CLC) to chromosome 19 and the human ribonuclease 2 (eosinophil-derived neurotoxin) and ribonuclease 3 (eosinophil cationic protein) genes (RNS2 and RNS3) to chromosome 14."
    Mastrianni D.M., Eddy R.L., Rosenberg H.F., Corrette S.E., Shows T.B., Tenen D.G., Ackerman S.J.
    Genomics 13:240-242(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-28.
  3. "The genomic structure of the human Charcot-Leyden crystal protein gene is analogous to those of the galectin genes."
    Dyer K.D., Handen J.S., Rosenberg H.F.
    Genomics 40:217-221(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-28.
  4. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT VAL-28.
    Tissue: Placenta.
  5. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-28.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-28.
  8. "Comparative proteomics of nasal fluid in seasonal allergic rhinitis."
    Ghafouri B., Irander K., Lindbom J., Tagesson C., Lindahl M.
    J. Proteome Res. 5:330-338(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-23; 92-99 AND 135-141, ACETYLATION AT SER-2, LACK OF GLYCOSYLATION AT ASN-136, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function."
    Kubach J., Lutter P., Bopp T., Stoll S., Becker C., Huter E., Richter C., Weingarten P., Warger T., Knop J., Muellner S., Wijdenes J., Schild H., Schmitt E., Jonuleit H.
    Blood 110:1550-1558(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  10. Cited for: TISSUE SPECIFICITY.
  11. "Galectin-10, a potential biomarker of eosinophilic airway inflammation."
    Chua J.C., Douglass J.A., Gillman A., O'Hehir R.E., Meeusen E.N.
    PLoS ONE 7:E42549-E42549(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  12. "Crystal structure of human Charcot-Leyden crystal protein, an eosinophil lysophospholipase, identifies it as a new member of the carbohydrate-binding family of galectins."
    Leonidas D.D., Elbert B.L., Zhou Z., Leffler H., Ackerman S.J., Acharya K.R.
    Structure 3:1379-1393(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  13. "Selective recognition of mannose by the human eosinophil Charcot-Leyden crystal protein (galectin-10): a crystallographic study at 1.8 A resolution."
    Swaminathan G.J., Leonidas D.D., Savage M.P., Ackerman S.J., Acharya K.R.
    Biochemistry 38:13837-13843(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  14. "Charcot-Leyden crystal protein (galectin-10) is not a dual function galectin with lysophospholipase activity but binds a lysophospholipase inhibitor in a novel structural fashion."
    Ackerman S.J., Liu L., Kwatia M.A., Savage M.P., Leonidas D.D., Swaminathan G.J., Acharya K.R.
    J. Biol. Chem. 277:14859-14868(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 2-139, ABSENCE OF LYSOPHOSPHOLIPASE ACTIVITY, INTERACTION WITH CEL.

Entry informationi

Entry nameiLEG10_HUMAN
AccessioniPrimary (citable) accession number: Q05315
Secondary accession number(s): C5HZ13, C5HZ14, Q0VDE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2011
Last modified: November 26, 2014
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Forms hexagonal bipyramidal crystals, known as Charcot-Leyden crystals, in tissues and secretions from sites of eosinophil-associated inflammation and some myeloid leukemias.

Caution

Was originally thought to possess lysophospholipase activity but the absence of this activity has been shown by PubMed:11834744.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3