Q05315 (LPPL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eosinophil lysophospholipase
EC=3.1.1.5

Alternative name(s):
Charcot-Leyden crystal protein
Short name=CLC
Galectin-10
Short name=Gal-10
Lysolecithin acylhydrolase

Gene names

Name:

CLC

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have both lysophospholipase and carbohydrate-binding activities.
Catalytic activity	2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Subcellular location	Cytoplasmic granule. Note: Localized in granules from where it may be secreted or transported to other locations in the cell.
Tissue specificity	Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils.
Miscellaneous	Forms hexagonal bipyramidal crystals, known as Charcot-Leyden crystals, in tissues and secretions from sites of eosinophil-associated inflammation and some myeloid leukemias.
Sequence similarities	Contains 1 galectin domain.

Ontologies

Keywords
Biological process	Lipid degradation
Coding sequence diversity	Polymorphism
Ligand	Lectin
Molecular function	Hydrolase Serine esterase
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Traceable author statement. Source: ProtInc
Molecular function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW lysophospholipase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.6

Chain

2 – 142

141

Eosinophil lysophospholipase

PRO_0000076960

Regions

Domain

6 – 138

133

Galectin

Sites

Site

136

1

Not glycosylated

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.6

Natural variations

Natural variant

28

1

A → V. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5
Corresponds to variant rs17608 [ dbSNP | Ensembl ].

VAR_014765

Secondary structure

1

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142

Helix Strand Turn

Details...

Beta strand

6 – 11

6

Beta strand

19 – 28

10

Helix

30 – 32

3

Beta strand

35 – 45

11

Beta strand

50 – 57

8

Turn

58 – 60

3

Beta strand

61 – 68

8

Beta strand

76 – 78

3

Beta strand

89 – 95

7

Beta strand

97 – 104

8

Beta strand

107 – 113

7

Helix

118 – 120

3

Beta strand

123 – 137

15

Sequences

	Sequence		Length	Mass (Da)
	Q05315 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: 2744F0C082C6446E	FASTA	142	16,453
	10 20 30 40 50 60 MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI VFHFQVCFGR 70 80 90 100 110 120 RVVMNSREYG AWKQQVESKN MPFQDGQEFE LSISVLPDKY QVMVNGQSSY TFDHRIKPEA 130 140 VKMVQVWRDI SLTKFNVSYL KR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase). Similarities to IgE binding proteins and the S-type animal lectin superfamily." Ackerman S.J., Corrette S.E., Rosenberg H.F., Bennett J.C., Mastrianni D.M., Nicholson-Weller A., Weller P.F., Chin D.T., Tenen D.G. J. Immunol. 150:456-468(1993) [PubMed: 8419478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-18 AND 93-110, VARIANT VAL-28.
[2]	"Localization of the human eosinophil Charcot-Leyden crystal protein (lysophospholipase) gene (CLC) to chromosome 19 and the human ribonuclease 2 (eosinophil-derived neurotoxin) and ribonuclease 3 (eosinophil cationic protein) genes (RNS2 and RNS3) to chromosome 14." Mastrianni D.M., Eddy R.L., Rosenberg H.F., Corrette S.E., Shows T.B., Tenen D.G., Ackerman S.J. Genomics 13:240-242(1992) [PubMed: 1577491] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-28.
[3]	"The genomic structure of the human Charcot-Leyden crystal protein gene is analogous to those of the galectin genes." Dyer K.D., Handen J.S., Rosenberg H.F. Genomics 40:217-221(1997) [PubMed: 9119387] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-28.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-28.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-28.
[6]	"Comparative proteomics of nasal fluid in seasonal allergic rhinitis." Ghafouri B., Irander K., Lindbom J., Tagesson C., Lindahl M. J. Proteome Res. 5:330-338(2006) [PubMed: 16457599] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-23; 92-99 AND 135-141, ACETYLATION AT SER-2, LACK OF GLYCOSYLATION AT ASN-136, MASS SPECTROMETRY.
[7]	"Crystal structure of human Charcot-Leyden crystal protein, an eosinophil lysophospholipase, identifies it as a new member of the carbohydrate-binding family of galectins." Leonidas D.D., Elbert B.L., Zhou Z., Leffler H., Ackerman S.J., Acharya K.R. Structure 3:1379-1393(1995) [PubMed: 8747464] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[8]	"Selective recognition of mannose by the human eosinophil Charcot-Leyden crystal protein (galectin-10): a crystallographic study at 1.8 A resolution." Swaminathan G.J., Leonidas D.D., Savage M.P., Ackerman S.J., Acharya K.R. Biochemistry 38:13837-13843(1999) [PubMed: 10529229] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Galectin-10

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L01664 mRNA. Translation: AAA36190.1.
L01665 Genomic DNA. Translation: AAC37530.1.
U68398 , U68393, U68395, U68396 Genomic DNA. Translation: AAC51157.1.
AC005393 Genomic DNA. Translation: AAC28912.1.
AC006133 Genomic DNA. No translation available.
BC119711 mRNA. Translation: AAI19712.1.
BC119712 mRNA. Translation: AAI19713.1.

IPI

IPI00216071.

PIR

A46523.

RefSeq

NP_001819.2. NM_001828.5.

UniGene

Hs.889.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G86	X-ray	1.80	A	1-142	[»]
1HDK	X-ray	1.80	A	2-139	[»]
1LCL	X-ray	1.80	A	1-142	[»]
1QKQ	X-ray	1.80	A	2-141	[»]

ProteinModelPortal

Q05315.

SMR

Q05315. Positions 2-142.

ModBase

Search...

Protein-protein interaction databases

STRING

Q05315.

Polymorphism databases

DMDM

547870.

Proteomic databases

PRIDE

Q05315.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000221804; ENSP00000221804; ENSG00000105205.

GeneID

1178.

KEGG

hsa:1178.

UCSC

uc002omh.1. human.

Organism-specific databases

CTD

1178.

GeneCards

GC19M040221.

H-InvDB

HIX0040154.

HGNC

HGNC:2014. CLC.

HPA

HPA041751.

MIM

153310. gene.

neXtProt

NX_Q05315.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG20158.

GeneTree

ENSGT00530000064497.

HOGENOM

HBG099916.

HOVERGEN

HBG006255.

InParanoid

Q05315.

OMA

QVWRDIS.

OrthoDB

EOG47H5RQ.

Enzyme and pathway databases

BRENDA

3.1.1.5. 2681.

Gene expression databases

ArrayExpress

Q05315.

Bgee

Q05315.

CleanEx

HS_CLC.

Genevestigator

Q05315.

GermOnline

ENSG00000105205. Homo sapiens.

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013320. ConA-like_subgrp.
IPR001079. Galectin_CRD.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

KO

K13334.

Pfam

PF00337. Gal-bind_lectin. 1 hit.
[Graphical view]

SMART

SM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

PROSITE

PS51304. GALECTIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

4868.

SOURCE

Search...

Entry information

Entry name

LPPL_HUMAN

Accession

Primary (citable) accession number: Q05315
Secondary accession number(s): Q0VDE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 11, 2011
Last modified:	January 25, 2012
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.