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Q05315 (LEG10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galectin-10

Short name=Gal-10
Alternative name(s):
Charcot-Leyden crystal protein
Short name=CLC
Eosinophil lysophospholipase
Lysolecithin acylhydrolase
Gene names
Name:CLC
Synonyms:LGALS10, LGALS10A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length142 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg). Ref.9

Subunit structure

Interacts with CEL. Ref.14

Subcellular location

Cytoplasmcytosol. Cytoplasmic granule. Note: Localized in granules from where it may be secreted or transported to other locations in the cell. Ref.9

Tissue specificity

Expressed abundantly in the bone marrow. Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. Expressed in CD25-positive regulatory T-cells (Treg) (at protein level). Found in intestinal tissue from patients with Celiac disease, expression is directly related to the histological grade of mucosal damage and to the number of eosinophils found in the duodenal lesion (at protein level). Found in sputum of patients with eosinophilic inflammatory diseases such as asthma (at protein level). Ref.4 Ref.9 Ref.10 Ref.11

Miscellaneous

Forms hexagonal bipyramidal crystals, known as Charcot-Leyden crystals, in tissues and secretions from sites of eosinophil-associated inflammation and some myeloid leukemias.

Sequence similarities

Contains 1 galectin domain.

Caution

Was originally thought to possess lysophospholipase activity but the absence of this activity has been shown by Ref.14.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 142141Galectin-10
PRO_0000076960

Regions

Domain6 – 138133Galectin

Sites

Site1361Not glycosylated

Amino acid modifications

Modified residue21N-acetylserine Ref.8

Natural variations

Natural variant281A → V. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.7
Corresponds to variant rs17608 [ dbSNP | Ensembl ].
VAR_014765

Secondary structure

......................... 142
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q05315 [UniParc].

Last modified January 11, 2011. Version 3.
Checksum: 2744F0C082C6446E

FASTA14216,453
        10         20         30         40         50         60 
MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI VFHFQVCFGR 

        70         80         90        100        110        120 
RVVMNSREYG AWKQQVESKN MPFQDGQEFE LSISVLPDKY QVMVNGQSSY TFDHRIKPEA 

       130        140 
VKMVQVWRDI SLTKFNVSYL KR 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase). Similarities to IgE binding proteins and the S-type animal lectin superfamily."
Ackerman S.J., Corrette S.E., Rosenberg H.F., Bennett J.C., Mastrianni D.M., Nicholson-Weller A., Weller P.F., Chin D.T., Tenen D.G.
J. Immunol. 150:456-468(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-18 AND 93-110, VARIANT VAL-28.
[2]"Localization of the human eosinophil Charcot-Leyden crystal protein (lysophospholipase) gene (CLC) to chromosome 19 and the human ribonuclease 2 (eosinophil-derived neurotoxin) and ribonuclease 3 (eosinophil cationic protein) genes (RNS2 and RNS3) to chromosome 14."
Mastrianni D.M., Eddy R.L., Rosenberg H.F., Corrette S.E., Shows T.B., Tenen D.G., Ackerman S.J.
Genomics 13:240-242(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-28.
[3]"The genomic structure of the human Charcot-Leyden crystal protein gene is analogous to those of the galectin genes."
Dyer K.D., Handen J.S., Rosenberg H.F.
Genomics 40:217-221(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-28.
[4]"A primate subfamily of galectins expressed at the maternal-fetal interface that promote immune cell death."
Than N.G., Romero R., Goodman M., Weckle A., Xing J., Dong Z., Xu Y., Tarquini F., Szilagyi A., Gal P., Hou Z., Tarca A.L., Kim C.J., Kim J.S., Haidarian S., Uddin M., Bohn H., Benirschke K. expand/collapse author list , Santolaya-Forgas J., Grossman L.I., Erez O., Hassan S.S., Zavodszky P., Papp Z., Wildman D.E.
Proc. Natl. Acad. Sci. U.S.A. 106:9731-9736(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT VAL-28.
Tissue: Placenta.
[5]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-28.
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-28.
[8]"Comparative proteomics of nasal fluid in seasonal allergic rhinitis."
Ghafouri B., Irander K., Lindbom J., Tagesson C., Lindahl M.
J. Proteome Res. 5:330-338(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-23; 92-99 AND 135-141, ACETYLATION AT SER-2, LACK OF GLYCOSYLATION AT ASN-136, IDENTIFICATION BY MASS SPECTROMETRY.
[9]"Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function."
Kubach J., Lutter P., Bopp T., Stoll S., Becker C., Huter E., Richter C., Weingarten P., Warger T., Knop J., Muellner S., Wijdenes J., Schild H., Schmitt E., Jonuleit H.
Blood 110:1550-1558(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]"Galectin-10, eosinophils, and celiac disease."
De Re V., Simula M.P., Cannizzaro R., Pavan A., De Zorzi M.A., Toffoli G., Canzonieri V.
Ann. N. Y. Acad. Sci. 1173:357-364(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[11]"Galectin-10, a potential biomarker of eosinophilic airway inflammation."
Chua J.C., Douglass J.A., Gillman A., O'Hehir R.E., Meeusen E.N.
PLoS ONE 7:E42549-E42549(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[12]"Crystal structure of human Charcot-Leyden crystal protein, an eosinophil lysophospholipase, identifies it as a new member of the carbohydrate-binding family of galectins."
Leonidas D.D., Elbert B.L., Zhou Z., Leffler H., Ackerman S.J., Acharya K.R.
Structure 3:1379-1393(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[13]"Selective recognition of mannose by the human eosinophil Charcot-Leyden crystal protein (galectin-10): a crystallographic study at 1.8 A resolution."
Swaminathan G.J., Leonidas D.D., Savage M.P., Ackerman S.J., Acharya K.R.
Biochemistry 38:13837-13843(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[14]"Charcot-Leyden crystal protein (galectin-10) is not a dual function galectin with lysophospholipase activity but binds a lysophospholipase inhibitor in a novel structural fashion."
Ackerman S.J., Liu L., Kwatia M.A., Savage M.P., Leonidas D.D., Swaminathan G.J., Acharya K.R.
J. Biol. Chem. 277:14859-14868(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 2-139, ABSENCE OF LYSOPHOSPHOLIPASE ACTIVITY, INTERACTION WITH CEL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L01664 mRNA. Translation: AAA36190.1.
L01665 Genomic DNA. Translation: AAC37530.1.
U68398 expand/collapse EMBL AC list , U68393, U68395, U68396 Genomic DNA. Translation: AAC51157.1.
FJ613334 mRNA. Translation: ACR09636.1.
FJ613335 mRNA. Translation: ACR09637.1.
AC005393 Genomic DNA. Translation: AAC28912.1.
AC006133 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW56914.1.
BC119711 mRNA. Translation: AAI19712.1.
BC119712 mRNA. Translation: AAI19713.1.
PIRA46523.
RefSeqNP_001819.2. NM_001828.5.
UniGeneHs.889.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G86X-ray1.80A1-142[»]
1HDKX-ray1.80A2-139[»]
1LCLX-ray1.80A1-142[»]
1QKQX-ray1.80A1-142[»]
ProteinModelPortalQ05315.
SMRQ05315. Positions 2-142.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000221804.

PTM databases

PhosphoSiteQ05315.

Polymorphism databases

DMDM317373429.

Proteomic databases

PaxDbQ05315.
PRIDEQ05315.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000221804; ENSP00000221804; ENSG00000105205.
GeneID1178.
KEGGhsa:1178.
UCSCuc002omh.3. human.

Organism-specific databases

CTD1178.
GeneCardsGC19M040221.
H-InvDBHIX0040154.
HGNCHGNC:2014. CLC.
HPAHPA041751.
MIM153310. gene.
neXtProtNX_Q05315.
PharmGKBPA26541.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307598.
HOGENOMHOG000015090.
HOVERGENHBG006255.
InParanoidQ05315.
KOK13334.
OMAFHTEMKE.
OrthoDBEOG7WQ7V1.
PhylomeDBQ05315.
TreeFamTF315551.

Enzyme and pathway databases

BRENDA3.1.1.5. 2681.

Gene expression databases

ArrayExpressQ05315.
BgeeQ05315.
CleanExHS_CLC.
GenevestigatorQ05315.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR001079. Galectin_CRD.
[Graphical view]
PfamPF00337. Gal-bind_lectin. 1 hit.
[Graphical view]
SMARTSM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS51304. GALECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ05315.
GeneWikiCLC_(gene).
GenomeRNAi1178.
NextBio35482744.
PROQ05315.
SOURCESearch...

Entry information

Entry nameLEG10_HUMAN
AccessionPrimary (citable) accession number: Q05315
Secondary accession number(s): C5HZ13, C5HZ14, Q0VDE3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM